Objective To investigate the effects of long non-coding RNA(lncRNA)THOR on glucose metabolism reprogram-ming,migration and invasion of colorectal cancer(CRC)cells,and its targeted regulatory relationship with fructose-1,6-bisphosphate aldolase C(ALDOC).Methods Real-time fluorescence quantitative polymerase chain reaction(RT-qPCR)was used to detect the expression of THOR in CRC cell lines.THOR specific siRNA was transfected into HCT116 and SW480 cells to silence the expression of THOR,and the effects of THOR on the invasion and migration ability of CRC cells was detected by cell scratch assay and Transwell assay.The effect of THOR on glucose metabolic reprogramming in CRC cells was investigated by measuring the glucose intake and pyruvate pro-duction in CRC cells,and the expression levels of ALDOC mRNA and protein were detected by RT-qPCR and western blot.Results The results of RT-qPCR showed that the expression levels of THOR in HCT116 and SW480 cells were significantly higher than those in normal human intestinal epithelial cells NCM460.After the silencing of THOR,the cell scratch assay and Transwell assay showed that the migration and invasion ability of HCT116 and SW480 cells were inhibited,and the glucose uptake and pyruvate yield were decreased.The results of RT-qPCR and western blot showed that the expression levels of ALDOC mRNA and protein were significantly down-regulated,the difference was statistically significant(P＜0.05).Conclusion lncRNA THOR is highly expressed in CRC cells,which may affect the glucose metabolism reprogramming by targeting ALDOC to promote the migration and invasion of CRC cells.

Osteoarthritis (OA) is one of the most common chronic diseases in the world. However, current treatment modalities mainly relieve pain and inhibit cartilage degradation, but do not promote cartilage regeneration. In this study, we show that G protein-coupled receptor class C group 5 member B (GPRC5B), an orphan G-protein-couple receptor, not only inhibits cartilage degradation, but also increases cartilage regeneration and thereby is protective against OA. We observed that Gprc5b deficient chondrocytes had an upregulation of cartilage catabolic gene expression, along with downregulation of anabolic genes in vitro. Furthermore, mice deficient in Gprc5b displayed a more severe OA phenotype in the destabilization of the medial meniscus (DMM) induced OA mouse model, with upregulation of cartilage catabolic factors and downregulation of anabolic factors, consistent with our in vitro findings. Overexpression of Gprc5b by lentiviral vectors alleviated the cartilage degeneration in DMM-induced OA mouse model by inhibiting cartilage degradation and promoting regeneration. We also assessed the molecular mechanisms downstream of Gprc5b that may mediate these observed effects and identify the role of protein kinase B (AKT)-mammalian target of rapamycin (mTOR)-autophagy signaling pathway. Thus, we demonstrate an integral role of GPRC5B in OA pathogenesis, and activation of GPRC5B has the potential in preventing the progression of OA.

This study compared the effects on bone metabolism and morphology of pathological obesity induced by excessive fat intake in a non-hibernator (mice) versus healthy obesity due to pre-hibernation fattening in a hibernator (ground squirrels). Kunming mice were fed a high-fat diet to provide a model of pathological obesity (OB group). Daurian ground squirrels fattened naturally in their pre-hibernation season (PRE group) were used as a healthy obesity model. Micro-computed tomography (micro-CT) and three-point bending tests were used to determine the microstructure and mechanical properties of bone. Western blots were used to analyze protein expression levels related to bone metabolism (Runt-related transcription factor 2 (RunX2), osteocalcin (OCN), alkaline phosphatase (ALP), osteoprotegerin (OPG), receptor activator of nuclear factor-‍κB ligand (RANKL), cathepsin K, matrix metallopeptidase 9 (MMP9), patched protein homolog 1 (Ptch1), phosphorylated β‍-‍catenin (P‍-‍β‍-‍catenin), and glycogen synthase kinase-3β (GSK-3β)). Compared with controls, there was no obvious bone loss in the OB mice, and the stiffness of the femur was increased significantly. Compared with summer active squirrels, bone formation was enhanced but the mechanical properties did not change in the PRE group squirrels. In OB mice, western blots showed significantly increased expression levels of all proteins except RunX2, OPG, and Ptch1. PRE ground squirrels showed significantly increased expression of most proteins except OCN and Ptch1, which decreased significantly, and P‍-‍β‍-‍catenin and OPG, which did not change. In conclusion, for non-hibernating mice, moderate obesity had a certain protective effect on bones, demonstrating two-way regulation, increasing both bone loss and bone formation. For pre-hibernating ground squirrels, the healthy obesity acquired before hibernation had a positive effect on the microstructure of bones, and also enhanced the expression levels of proteins related to bone formation, bone resorption, and Wnt signaling.
Mice ; Animals ; Hibernation ; Core Binding Factor Alpha 1 Subunit/metabolism* ; Glycogen Synthase Kinase 3 beta/metabolism* ; Diet, High-Fat ; X-Ray Microtomography ; Sciuridae/metabolism* ; Obesity

Objective:To investigate the status quo and influence factors of job burnout among nursing assistants in geriatric ward.Methods:From January to October 2021, a total of 200 nursing assistants were selected in geriatric ward of Beijing Friendship Hospital Affiliated to Capital Medical University by the convenient sampling method. Questionnaires were used to collect the general information of nursing assistants, such as gender, age, education level, working years, and time of taking care current elderly patients as well as the general information of the elderly patients, including gender, age, body mass index (BMI), activity of daily living, comorbidity index and so on. The Chinese version of the Maslach Occupational Questionnaire (MBI-GS) was used to investigate the status quo of job burnout among nursing assistants in geriatric ward. Univariate and multivariate Logistic regression were used to analyze the influencing factors of job burnout among nursing assistants in geriatric ward.Results:Among 200 nursing assistants, the incidence of job burnout was 49.00% (98/200), including 64.29% (63/98) mild burnout, 23.47% (23/98) moderate burnout, 12.24% (12/98) severe burnout. There were significant differences in working years, the time of taking care current elderly patients, average monthly income, working hours in one year, degree of respect from family members, job satisfaction and whether they had experienced provocation behaviors among nursing assistants with different job burnout levels ( P<0.05). There were significant differences in elderly patients' age, vision status, pressure sore score, Instrumental Activity of Daily Living (IADL) score, Activity of Daily Living (ADL) score among nursing assistants with different job burnout levels ( P<0.05). Logistic regression analysis showed that working years, average monthly income, annual working hours, degree of respect from family members, experienced aggressive behavior, and visual status, pressure sore score, IADL score, ADL score of the elderly patients were the influencing factors of job burnout among nursing assistants in geriatric ward ( P<0.05) . Conclusions:Nursing assistants in geriatric ward generally have job burnout, mainly mild burnout, and working years, average monthly income, annual working hours, degree of respect from family members, experienced aggressive behavior, and visual status, pressure ulcer score, IADL score and ADL score of the elderly patients were the influencing factors of job burnout.