ObjectiveTo investigate the imaging features of calcium salt deposition and serological markers in patients with alveolar echinococcosis through a retrospective analysis， as well as independent risk factors for the degree of calcium salt deposition in lesions， and to provide a basis for assessing disease process. MethodsA retrospective analysis was performed for the imaging and clinical data of 107 patients with alveolar echinococcosis who were admitted to The First Affiliated Hospital of Shihezi University from December 2023 to June 2025， and according to the volume of calcium salt deposition， they were divided into non-deposition group with 16 patients， mild deposition group with 52 patients， moderate deposition group with 16 patients， and severe deposition group with 23 patients. A one-way analysis of variance or the Kruskal-Wallis H test was used for comparison of continuous data between groups， and the χ² test or Fisher’s exact test was used for comparison of categorical data between groups. The four groups were further combined into the low deposition group （no/mild deposition） and the high deposition group （moderate/severe deposition）. A binary logistic regression analysis was used to investigate the independent influencing factors for calcium salt deposition， and a predictive model was established. The receiver operating characteristic （ROC） curve was used to assess the predictive performance of the model， and the Bootstrap method was used for internal validation. ResultsThere were significant differences between the four groups in sex distribution， involvement of other sites， white blood cell count， lymphocyte percentage， fibrinogen， uric acid， sodium ion， chloride ion， and calcium ion （all P<0.05）. The univariate analysis showed that there were significant differences between the four groups in sex， involvement of other sites， white blood cell count， lymphocyte percentage， fibrinogen， alanine aminotransferase， albumin， creatinine， uric acid， sodium ion， chloride ion， and calcium ion （all P<0.1）. The multi-collinearity diagnosis showed that the VIF values for all continuous variables ranged from 1.104 to 1.760， suggesting that collinearity did not affect modeling. An ordinal logistic regression model was established based on sex， involvement of other sites， calcium ion， lymphocyte percentage， and uric acid. The multivariate analysis showed that lymphocyte percentage （odds ratio ［OR］=1.106， 95% confidence interval ［CI］： 1.041 — 1.174， P=0.001） and blood calcium level （OR=0.005， 95%CI： 0.000 —0.230， P=0.007） were independent influencing factors for the degree of calcium salt deposition. The regression equation was established as Logit（P）=8.231 + 0.100 × lymphocyte percentage -5.344 × calcium ion. The ROC curve analysis showed that the model had an area under the ROC curve of 0.716， with a Youden index of 0.353， a sensitivity of 1.000， and a specificity of 0.353. The Hosmer-Lemeshow test showed that the model had poor calibration （χ²=20.688， P=0.008）. The Bootstrap method with 1000 repeated samples showed that the estimated values of lymphocyte percentage （OR=1.106， 95%CI： 1.049 — 1.186， P=0.002） and calcium ion （OR=0.005， 95%CI： 0.000 — 0.214， P=0.010） were consistent with the original model， and the confidence intervals did not include 1， which further supported the reliability of the model. ConclusionBoth lymphocyte percentage and blood calcium level are independent influencing factors for calcium salt deposition in alveolar echinococcosis， and the degree of calcium salt deposition in alveolar echinococcosis lesions increases with the reduction in blood calcium level and the increase in lymphocyte percentage.

Objective To construct a recombinant poxvirus vector vaccine, rVTTδTK-RBD, and to evaluate its safety and immunogenicity. Methods The receptor-binding domain (RBD) gene was synthesized with reference to the gene sequence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and was inserted into the polyclonal site of the self-constructed recombinant plasmid pSTKE, to construct the recombinant poxvirus shuttle vector pSTKE-RBD. This was then transfected into BHK-21 cells pre-infected with the vaccinia virus Tiantan strain (VTT). The recombinant poxvirus rVTTδTK-RBD was successfully obtained after several rounds of fluorescence phage screening. The effect of rVTTδTK-RBD on the body mass of BALB/c mice was detected after immunizing mice by intra-nasal vaccination. The levels of specific and neutralizing antibodies produced by rVTTδTK-RBD on BALB/c mice were analyzed after immunizing mice intramuscularly. The effect of rVTTδTK-RBD on T cell subsets in BALB/c mice was detected by flow cytometry. Results Through homologous recombination, enhanced green fluorescent protein (EGFP) screening marker, and multiple rounds of fluorescent phosphorescence phage screening, a recombinant poxvirus rVTTδTK-RBD, expressing RBD with deletions in the thymidine kinase (TK) gene, was successfully obtained, which was validated by PCR. The in vivo experiments on BALB/c mice showed that rVTTδTK-RBD was highly immunogenic against SARS-CoV-2 and significantly reduced toxicity to the body compared to the parental strain VTT. Conclusion The recombinant poxvirus vaccine rVTTδTK-RBD against SARS-CoV-2 is successfully constructed and obtained, with its safety and immunogenicity confirmed through various experiments.
Animals ; Mice ; SARS-CoV-2/genetics* ; COVID-19 ; Vaccines, Synthetic/genetics* ; Genes, Reporter ; Bacteriophages ; Mice, Inbred BALB C

Objective: To analysis the epidemiological characteristics of overweight and obesity among middle school students in Taizhou from 2013 to 2020, to provide support for prevention and control efforts. Methods: Through stratified sampling, one primary school, one junior middle school and one senior high school were randomly selected from nine counties (cities and districts) in Taizhou, and continuous monitoring was conducted in all participants Height, weight and other variables were assessed and body mass index was calculated. The epidemic characteristics were analyzed according to the detection rate, fixed base ratio, sequential growth ratio and average growth rate. Results: From 2013 to 2020, the overall overweight rate among primary and middle school students in Taizhou was 14.5%(36 592/252 583), and the obesity rate was 11.2%(28 256/252 583). The rates of overweight and obesity increased, with average annual growth rates of 1.9% and 5.5%, respectively; thus, the rate of obesity increased more rapidly. The obesity rate was higher among boys (13.2%) than girls (9.0%)( χ 2=1 119.57, P <0.01), and the average annual growth rate was higher among girls than boys (6.1%, 5.2%, respectively). The rate of overweight among boys (17.6%) was higher than that among girls (10.9%)( χ 2=2 307.35, P <0.01). The average annual growth rate of overweight in girls was 2.3% and 1.7%, respectively. The obesity rate among primary school students (17.5%) was higher than that among middle school (9.7%) and high school (4.9%) students( χ 2=7 291.33, P <0.01). The average annual growth rate in students in middle school was fastest, followed by those in high and primary schools (6.5%, 3.9% and 2.6%, respectively). The rate of overweight in primary school students ( 15.8 %) was higher than that in middle school students (15.3%), and both were higher than that in high school students(12.2%)( χ 2=521.06, P <0.01). The average annual growth rate was also fastest in students in middle school, followed by high and primary schools (2.4%, 2.2% and 0.6%, respectively). Conclusion The detection rate of overweight and obesity among primary and middle school students in Taizhou is high and increasing rapidly, indicating high pressure on prevention and control. Boys and primary school students are the key target groups for prevention and control. Comprehensive prevention and control strategies should be adopted specifically.

Objective : To investigate the correlation between obstructive sleep apnea hypopnea syndrome (OSAHS ) and hypertension and its risk factors ,so as to provide basis for prevention and treatment of OSAHS accompanied by hypertension . Methods : A total of 108 OSAHS patients were enrolled and the general conditions were recorded . The blood pressure at 22 ∶ 00 before sleep and that at 8 ∶ 00 . next morning was determined routinely ,and the blood lipid level was detected . According to apnea hypoapnea index (AHI) ,the patients were divided into three groups ,the mild group (5/h ≤ AHI < 15/h) with 31 cases ,the moderate group (15/h ≤ AHI < 40/h) with 36 cases ,and the severe group (AHI ≥ 40 /h) with 41 cases . The levels of blood pressure were compared among the groups . According to whether OSAHS was accompanied by hypertension ,the patients were divided into simple OSAHS group and OSAHS accompanied by hypertension group . The relevant factors associated with OSAHS accompanied by hypertension were screened out by single factor analysis ,and then the multiple Logistic regression analysis was conducted .Results : (1)Among 108 patients with OSAHS ,60 cases(55 .6% ) were accompanied by hypertension ,including 15 cases of mild group(48 .4% ,15/31) ,19 cases of moderate group (52 .8% ,19/36) ,and 26 cases of severe group (63 .4% ,26/41 ) . There was no significant difference regarding the prevalence rate of hypertension among the three groups (P > 0 .05) .There were significant differences regarding systolic blood pressure(SBP) and diastolic blood pressure (DBP) at 22 ∶ 00 and 8 ∶ 00 among the three groups (P < 0 .05) ;SBP and DBP at 22 ∶ 00 and 8 ∶ 00 in severe group were significantly higher than those in mild group (P< 0 .05) .SBP at 22 ∶ 00 and 8 ∶ 00 in severe group was significantly higher than that in moderate group (P< 0 .05) .(2) Single factor analysis showed that age ,body mass index (BMI) ,smoking rate ,abdominal circumference ,AHI ,total cholesterol(TC) ,triglyceride(TG ) and low density lipoprotein cholesterol (LDL-C) in OSAHS accompanied by hypertension group were significantly higher than those in simple OSAHS group ,while HDL-C was significantly lower than that in simple OSAHS group ( P < 0 .05 ) . (3 ) Multivariate Logistic regression analysis showed that BMI ,AHI ,TC were the risk factors for OSAHS accompanied by hypertension .Conclusions :The level of blood pressure in patients with OSAHS was accordance with the disease severity ,while BMI ,AHI ,TC were the risk factors for OSAHS accompanied by hypertension .

The regulatory effects of niacinamide (Nia) on intervertebral disc (IVD) aggrecan in vitro was investigated. Chiba's 10 ng/mL interleukin-1 (IL-1)-induced rabbit IVD degeneration model in vitro was established. 0.5, 0.25 and 0.05 mg/mL Nia was added to normal and degenerated IVDs for intervention. On the first and second week after intervention, safranin O-fast green staining intensity and glycosaminoglycan (GS) content were measured. The expression of aggrecan core protein was detected by RT-PCR. The results showed: (1) After treatment with 0.5 mg/mL Nia for one week, the GS content in nucleus pulposus (NP) was increased by 44.8 % as compared with control group (P＜0.01); The GS content in IL-1 induction groups was increased with the increase of Nia concentrations: After treatment with 0.5 mg/mL for one week, the GS content in NP was increased by 68.3 % as compared with control group (P＜0.01). After two weeks, GS content in NP and fibrous rings was still higher than in control group at the same period (P＜0.01)and untreated group (P＜0.01). (2) Safranin O-fast green staining revealed that with the increase of Nia concentrations, staining density in NP and fibrous rings was increased and histological structure damage to IVDs by IL-1β was alleviated. (3) RT-PCR showed that the expression of core protein gene in IL-1β-induced degenerated IVDS was increased with the increase of Nia concentrations.It was concluded that under conditions in vitro, Nia could up-regulate the expression of aggrecan in IVDs and protect IVDs from IL-1β-induced degeneration at least partially, which offers a potential choice for IVD degeneration clinical therapy.