Objective:To explore the effects of Ziyu Ointment on granulation tissue,TGF-β and downstream Smad protein in the process of wound healing in rats by constructing a rat model of back trauma.Methods:A rat model of back trauma was constructed,and 100 SD male rats were separated into healthy group,back trauma group,Ziyu Ointment treatment group,petrolatum treatment group,Ziyu Ointment+TGF-β1 activation group,with 20 per group.Wound formation surgery was performed,and wound healing of rats in each group was recorded at 5th,10th,and 15th day after operation;the levels of IL-6,IL-1β and TNF-α in the serum of rats in each group were detected;the granulation tissue formed by wound healing at 15th day was selected for HE and Masson staining,the proportion of blood vessel area and collagen area of granulation tissue of rats in each group were analyzed and calculated;the mRNA expression levels of TGF-β1 and Smad3 in granulation tissues of rats in each group were detected by fluorescence quantitative PCR;the expression levels of TGF-β1,Smad3 and p-Smad3 in granulation tissues of rats in each group were detected by Western blot.Results:The healthy group rats had complete epidermal tissue;the levels of serum IL-6,IL-1β,TNF-α,the expression of TGF-β1 mRNA and protein in the wound granulation tissue,and the phosphorylation of Smad3 in the back trauma group were obviously higher than the healthy group(P<0.05);compared with back trauma group,the wound healing rate,proportion of collagen area,and propor-tion of blood vessel area in Ziyu Ointment treatment group and petrolatum treatment group were obviously increased,the serum IL-6,IL-1β,TNF-α contents,TGF-β1 mRNA and protein expression,and Smad3 phosphorylation were obviously decreased(P<0.05);compared with Ziyu Ointment treatment group,the petrolatum treatment group,Ziyu Ointment+TGF-β1 activation group had obviously lower wound healing rate,collagen area percentage,and blood vessel area percentage,and obviously lower serum IL-6,IL-1β,TNF-α contents,TGF-β1 mRNA and protein expression and Smad3 phosphorylation(P<0.05).Conclusion:Ziyu Ointment can inhibit the expression of related genes and proteins by regulating the TGF-β/Smad signal pathway,thereby alleviating inflammation and promoting wound healing.

Objective:To explore the effects of Ziyu Ointment on granulation tissue,TGF-β and downstream Smad protein in the process of wound healing in rats by constructing a rat model of back trauma.Methods:A rat model of back trauma was constructed,and 100 SD male rats were separated into healthy group,back trauma group,Ziyu Ointment treatment group,petrolatum treatment group,Ziyu Ointment+TGF-β1 activation group,with 20 per group.Wound formation surgery was performed,and wound healing of rats in each group was recorded at 5th,10th,and 15th day after operation;the levels of IL-6,IL-1β and TNF-α in the serum of rats in each group were detected;the granulation tissue formed by wound healing at 15th day was selected for HE and Masson staining,the proportion of blood vessel area and collagen area of granulation tissue of rats in each group were analyzed and calculated;the mRNA expression levels of TGF-β1 and Smad3 in granulation tissues of rats in each group were detected by fluorescence quantitative PCR;the expression levels of TGF-β1,Smad3 and p-Smad3 in granulation tissues of rats in each group were detected by Western blot.Results:The healthy group rats had complete epidermal tissue;the levels of serum IL-6,IL-1β,TNF-α,the expression of TGF-β1 mRNA and protein in the wound granulation tissue,and the phosphorylation of Smad3 in the back trauma group were obviously higher than the healthy group(P<0.05);compared with back trauma group,the wound healing rate,proportion of collagen area,and propor-tion of blood vessel area in Ziyu Ointment treatment group and petrolatum treatment group were obviously increased,the serum IL-6,IL-1β,TNF-α contents,TGF-β1 mRNA and protein expression,and Smad3 phosphorylation were obviously decreased(P<0.05);compared with Ziyu Ointment treatment group,the petrolatum treatment group,Ziyu Ointment+TGF-β1 activation group had obviously lower wound healing rate,collagen area percentage,and blood vessel area percentage,and obviously lower serum IL-6,IL-1β,TNF-α contents,TGF-β1 mRNA and protein expression and Smad3 phosphorylation(P<0.05).Conclusion:Ziyu Ointment can inhibit the expression of related genes and proteins by regulating the TGF-β/Smad signal pathway,thereby alleviating inflammation and promoting wound healing.

Radiomics is a non-invasive method to extract valuable features from computed tomography(CT)images to characterize the correlation between tumor phenotype and clinical treatment outcomes,which is of great significance in the evaluation of the efficacy of non-small cell lung cancer(NSCLC).This paper reviews the research methods of CT Radiomics in the evaluation of curative effect of NSCLC.Firstly,the research content of CT radiomics in NSCLC is summarized.Then,from the perspective of different treatment methods,such as namely radiotherapy and chemotherapy,targeted therapy and immunotherapy,the research methods of CT radiomics in the evaluation of NSCLC efficacy were summarized,and the CT radiomics was compared with other commonly used efficacy evaluation systems.Finally,the development trend and improvement of the application of CT radiomics in the evaluation of NSCLC curative effect were summarized and prospected.

Objective: To study the influence of community mental rehabilitation based on Deming Cycle(PDCA) management model on the quality of life of patients with chronic schizophrenia. Methods: From January 2016 to June 2017, 100 patients with chronic schizophrenia who agreed to community management in Wuxi were randomly divided into study group(n=50) and control group(n=50). Both two groups received traditional community mental health services, meanwhile, the study group was provided with community mental rehabilitation based on PDCA model.All the patients were followed up for 1 year.The quality of life scales(SQLS), social disability screening schedule(SDSS) and family care index(APGAR) were assessed before and after one-year intervention. Results: At the end of one year after intervention, the scores of total quality of life, psychosocial dimension, motive and energy dimension, symptom and side effects in the observation group were (41.27±10.54)points, (38.37±10.84)points, (35.43±7.46)points, (20.71±7.34)points, respectively, which were lower than those in the control group[(51.98±7.43)points, (48.73±8.72)points, (47.67±6.98)points, (29.79±8.64)points] and before intervention[(54.37±5.87)points, (49.78±4.73)points, (48.57±6.87)points, (33.17±10.42)points](t=5.87, 5.27, 8.47, 5.66, 7.68, 6.82, 9.16, 6.91, all P<0.05). At the end of one year after intervention, the social function score[(6.78±2.84)points] of the observation group was significantly lower than that of the control group[(8.83±2.73)points]and before intervention[(8.97±2.18)points](t=3.68, 4.33, all P<0.05), and the family social care index score[(8.75±2.04)points]was significantly higher than that of the control group[(7.04±2.36)points] and before intervention[(6.76±2.14)points](t=-3.88, -4.76, all P<0.05). There was a positive correlation between SDSS score and SQLS score(r=0.41, P<0.05). There was a negative correlation between SQLS score and SDSS score(r=-0.35, P<0.05). Conclusion The community mental rehabilitation strategy based on PDCA model can improve the social function and family social care, effectively improve the quality of life of schizophrenic patients, while positively promote the rehabilitation of patients.