Dihydromyricetin is a flavonoid mainly found in Ampelopsis grossedentata,which has attracted extensive attention due to its diverse pharmacological activities in recent years.However,its low oral bioavailability limits its clinical application.In this article,the pharmacological effects of dihydromyricetin on antibacterial,antiviral,antitumor,inhibition of neuroinflammation,antioxidant,and hepatorenal protection were reviewed.By summarizing,it can be seen that the pharmacological effects of dihydromyricetin are related to the traditional efficacy of Ampelopsis grossedentata.Scholars have used new preparation technologie to design dosage forms and new drug delivery systems such as liposomes,nanoparticles,microemulsions and nanoemulsions,microcapsules and nanocapsules,which can improve the bioavailability and efficacy of dihydromyricetin.Which can provide reference for the development of new drugs.

Objective To understand and evaluate the overall status of nucleic acid detection efficacy for influenza A virus in the na-tionwide clinical laboratories of China,and discover and identify the potential issues to further improve the detection quality.Methods During 2024,the National Center for Clinical Laboratories distributed five samples to nationwide 1 367 participating laboratories.The detection efficacy of each participating laboratory was evaluated by calculating the overall percent agreement(OPA)of the test results using different detection reagents.Results The results of OPA,positive percent agreement(PPA)and negative percent agreement(NPA)of the five samples were 99.87％(6 826/6 835),99.89％(5 462/5 468),and 99.78％(1 364/1 367),respectively.No statistical difference of PPAs was observed between the H3N2 samples with different concentrations,between H1N1(2009)and H3N2 samples with equivalent concentration(1.0×104 copies/mL),and between seasonal H1N1 and H3N2 samples with equivalent concen-tration(1.0×105 copies/mL)(P＞0.05).Conclusion The results indicated the clinical laboratories in China exhibited robust efficacy in the molecular detection for two prevalent influenza A virus subtypes,i.e.,H1N1(2009)and H3N2.However,false-negative and false-positive results were encountered in a few laboratories.

Chronic hepatitis B virus (HBV) infection remains a major global public health concern. The persistent presence of intrahepatic covalently closed circular DNA (cccDNA) poses a major challenge to the complete cure of chronic HBV infection. Therefore, identifying reliable and effective serological surrogate markers for cccDNA holds great clinical significance in evaluating antiviral efficacy, predicting prognosis, and guiding the clinical management of chronic HBV-infected patients. In recent years, serum HBV RNA has emerged as a promising non-invasive alternative marker for cccDNA, offering the potential for monitoring disease progression and predicting prognosis in chronic HBV-infected patients. In this review, we summarize recent studies on HBV RNA, highlighting its ability to assess the immune and histological status of patients, and discussing its value in guiding the timing of antiviral therapy. Furthermore, we systematically summarize the clinical significance of HBV RNA in multiple domains: monitoring viral replication, evaluating antiviral treatment efficacy, predicting relapse after treatment cessation, and guiding new antiviral strategies. This review aims to provide clinicians with valuable insights for better utilizing this marker in the clinical management of chronic HBV infection.

Hepatic encephalopathy (HE) is a kind of neuropsychiatric syndrome caused by acute or chronic liver failure or portosystemic venous shunt (hereinafter referred to as portosystemic shunt), which can lead to the occurrence of functional impairment, personality and behavioral abnormalities, coma, and even death. Most patients with cirrhosis combined with HE have spontaneous portosystemic shunt (SPSS), especially those with recurrent or persistent HE. Internal medicine's current standard of treatment for HE associated with SPSS in cirrhotic patients is unsatisfactory, and even after treatment, recurrent HE episodes may still occur. Although interventional therapy has shown significant results and has been applied in clinical practice for many years for SPSS-associated HE, the number of treatment-related cases is relatively small, and there is a lack of large samples and well-designed research. Currently, interventional therapy for SPSS-associated HE in patients with cirrhosis is still under continuous exploration.

Objective To understand and evaluate the overall status of nucleic acid detection efficacy for influenza A virus in the na-tionwide clinical laboratories of China,and discover and identify the potential issues to further improve the detection quality.Methods During 2024,the National Center for Clinical Laboratories distributed five samples to nationwide 1 367 participating laboratories.The detection efficacy of each participating laboratory was evaluated by calculating the overall percent agreement(OPA)of the test results using different detection reagents.Results The results of OPA,positive percent agreement(PPA)and negative percent agreement(NPA)of the five samples were 99.87％(6 826/6 835),99.89％(5 462/5 468),and 99.78％(1 364/1 367),respectively.No statistical difference of PPAs was observed between the H3N2 samples with different concentrations,between H1N1(2009)and H3N2 samples with equivalent concentration(1.0×104 copies/mL),and between seasonal H1N1 and H3N2 samples with equivalent concen-tration(1.0×105 copies/mL)(P＞0.05).Conclusion The results indicated the clinical laboratories in China exhibited robust efficacy in the molecular detection for two prevalent influenza A virus subtypes,i.e.,H1N1(2009)and H3N2.However,false-negative and false-positive results were encountered in a few laboratories.

Dihydromyricetin is a flavonoid mainly found in Ampelopsis grossedentata,which has attracted extensive attention due to its diverse pharmacological activities in recent years.However,its low oral bioavailability limits its clinical application.In this article,the pharmacological effects of dihydromyricetin on antibacterial,antiviral,antitumor,inhibition of neuroinflammation,antioxidant,and hepatorenal protection were reviewed.By summarizing,it can be seen that the pharmacological effects of dihydromyricetin are related to the traditional efficacy of Ampelopsis grossedentata.Scholars have used new preparation technologie to design dosage forms and new drug delivery systems such as liposomes,nanoparticles,microemulsions and nanoemulsions,microcapsules and nanocapsules,which can improve the bioavailability and efficacy of dihydromyricetin.Which can provide reference for the development of new drugs.

Hepatic encephalopathy (HE) is a kind of neuropsychiatric syndrome caused by acute or chronic liver failure or portosystemic venous shunt (hereinafter referred to as portosystemic shunt), which can lead to the occurrence of functional impairment, personality and behavioral abnormalities, coma, and even death. Most patients with cirrhosis combined with HE have spontaneous portosystemic shunt (SPSS), especially those with recurrent or persistent HE. Internal medicine's current standard of treatment for HE associated with SPSS in cirrhotic patients is unsatisfactory, and even after treatment, recurrent HE episodes may still occur. Although interventional therapy has shown significant results and has been applied in clinical practice for many years for SPSS-associated HE, the number of treatment-related cases is relatively small, and there is a lack of large samples and well-designed research. Currently, interventional therapy for SPSS-associated HE in patients with cirrhosis is still under continuous exploration.

Objective To evaluate the significant clinical value of an intelligent dispatching platform based on the 120 command system and provide the basis for improving emergency service efficiency. Methods Convenience sampling was used to choose 356 patients who received pre-hospital care in the Jiaxing Emergency Center from August 2023 to May 2024 as research objects. The 178 patients who made phone calls to the 120 center from August to December 2023 (namely before the establishment of intelligent dispatching platform) served as a control group,while another 178 cases who made phone calls to the 120 center from January to May 2024 (namely after the establishment of intelligent dispatching platform) served as an observation group. The optimal ambulance was chosen and sent to receive the patients by the dispatcher himself in the control group,while the intelligent dispatching platform designed by the research team and computer engineers was used in the observation group. The platform was made up of multimodal receipt of alarms,accurate location of phone call places,matching and dispatching of ambulance resources,and collaborative management functions within the region. At the same time,geographic information,hospital information,and emergency vehicle information were completed to achieve united dispatching and whole-process quality control all over the region. The emergency response time,2 minutes ambulance dispatching rate,coincidence rate of medical treatment principles,and success rate of cardiopulmonary resuscitation in cities and rural areas were compared between the control group (before the establishment of intelligent dispatching platform) and the observation group (after the establishment of intelligent dispatching platform). Results The emergency response time in the observation group after the establishment of intelligent dispatching platform was shorter than that in the control group in cities (minutes:8.64±0.99 vs. 10.57±1.00,P<0.05),while there was no statistical significance between the observation group and the control group in rural areas (minutes:11.66±1.49 vs. 11.94±1.70,P>0.05). The 2 minutes ambulance dispatching rate higher than those in the control group[99.44％ (177/178) vs. 98.31％ (175/178)],there was no statistical significance (P>0.05),the coincidence rate of medical treatment principles in the observation group were significantly higher than those in the control group[98.88％ (176/178) vs. 89.89％ (160/178),P<0.05],For the observation group,the success rate of cardiopulmonary resuscitation in the observation group was slightly higher than that in the control group[18.92％ (7/37) vs. 13.51％ (5/37)],but there was no statistical significance (P>0.05). Conclusion The construction of an intelligent dispatching platform with the function of united dispatching,collaborative management of resources,and whole-process quality control all over the region can improve emergency service efficiency and effectiveness,thus promoting the development of pre-hospital care course.

Graves'ophthalmopathy(GO),also known as thyroid-associated ophthalmopathy,is an organ-specific au-toimmune disease that ranks first in the incidence of adult orbital diseases.GO has complex clinical manifestations,and dry eyes are a common cause of eye discomfort in patients with this disease.The pathogenesis of concurrent dry eyes is still unclear.It is currently believed that ocular surface damage in patients with GO is a direct factor leading to the occurrence and development of dry eyes.In addition,immunity and inflammation,and intestinal flora imbalance also play a role.Mul-tiple factors interact to form a vicious cycle,which further aggravates dry eyes.This article reviews the research results re-lated to the pathogenesis of GO complicated by dry eyes,aiming to provide ideas for clinical treatment and further research in the field.

Objective To evaluate the significant clinical value of an intelligent dispatching platform based on the 120 command system and provide the basis for improving emergency service efficiency. Methods Convenience sampling was used to choose 356 patients who received pre-hospital care in the Jiaxing Emergency Center from August 2023 to May 2024 as research objects. The 178 patients who made phone calls to the 120 center from August to December 2023 (namely before the establishment of intelligent dispatching platform) served as a control group,while another 178 cases who made phone calls to the 120 center from January to May 2024 (namely after the establishment of intelligent dispatching platform) served as an observation group. The optimal ambulance was chosen and sent to receive the patients by the dispatcher himself in the control group,while the intelligent dispatching platform designed by the research team and computer engineers was used in the observation group. The platform was made up of multimodal receipt of alarms,accurate location of phone call places,matching and dispatching of ambulance resources,and collaborative management functions within the region. At the same time,geographic information,hospital information,and emergency vehicle information were completed to achieve united dispatching and whole-process quality control all over the region. The emergency response time,2 minutes ambulance dispatching rate,coincidence rate of medical treatment principles,and success rate of cardiopulmonary resuscitation in cities and rural areas were compared between the control group (before the establishment of intelligent dispatching platform) and the observation group (after the establishment of intelligent dispatching platform). Results The emergency response time in the observation group after the establishment of intelligent dispatching platform was shorter than that in the control group in cities (minutes:8.64±0.99 vs. 10.57±1.00,P<0.05),while there was no statistical significance between the observation group and the control group in rural areas (minutes:11.66±1.49 vs. 11.94±1.70,P>0.05). The 2 minutes ambulance dispatching rate higher than those in the control group[99.44％ (177/178) vs. 98.31％ (175/178)],there was no statistical significance (P>0.05),the coincidence rate of medical treatment principles in the observation group were significantly higher than those in the control group[98.88％ (176/178) vs. 89.89％ (160/178),P<0.05],For the observation group,the success rate of cardiopulmonary resuscitation in the observation group was slightly higher than that in the control group[18.92％ (7/37) vs. 13.51％ (5/37)],but there was no statistical significance (P>0.05). Conclusion The construction of an intelligent dispatching platform with the function of united dispatching,collaborative management of resources,and whole-process quality control all over the region can improve emergency service efficiency and effectiveness,thus promoting the development of pre-hospital care course.