Objective To investigate the influence of a disintegrin and metalloproteinase 12(ADAM12),S100 calcium binding protein A8(S100A8),and serum tumor markers on chemotherapy outcomes and prognosis in patients with triple-negative breast cancer.Methods A totla of 300 patients with breast cancer admitted between January 2020 and January 2021 were included.Based on pathological immunohistochemistry findings,the patients were divided into a triple-negative group(n=98,triple-negative breast cancer)and a non-triple-negative group(n=202,non-triple-negative breast cancer).Serum tumor markers(carcinoembryonic antigen[CEA]and carbohydrate antigen 125[CA125]),the levels of ADAM12 and S100A8,and tissue protein expression levels of ADAM12 and S100A8 were compared between the two groups.The relationship between the protein levels of ADAM12 and S100A8 in the cancer tissues and the clinicopathological characteristics of the triple-negative group was analyzed.Differences in the protein levels of ADAM12 and S100A8 between patients with different chemotherapy outcomes(remission vs.non-remission)and different follow-up outcomes(survival vs.death)were analyzed.Kaplan-Meier survival analysis was used to examine the relationship between the protein levels of ADAM12 and S100A8 in cancer tissues and the prognosis.Results The protein expression levels of ADAM12 and S100A8 in cancer tissues from the triple-negative group were higher than those in the non-triple-negative group(P＜0.05).The protein expression levels of ADAM12 and S100A8 in cancer tissues were correlated with tumor diameter,histological grading,axillary lymph node metastasis,TNM staging,and differentiation degree in the triple-negative group(P＜0.05).The levels of CEA,CA125,ADAM12,and S100A8,as well as the protein expression levels of ADAM12 and S100A8 in cancer tissues in the non-remission group,were higher than those in the remission group(P＜0.05).Similarly,these markers were also significantly elevated in the death group compared to those in the survival group(P＜0.05).After 3 years of follow-up,the overall survival(OS)of patients with low ADAM12 expression was 36.0(8.0,36.0)months,while that of patients with high ADAM12 expression was 32.5(4.0,36.0)months,showing a statistically significant difference(log rank x2=12.913,P＜0.001).The OS of patients with low S100A8 expression was 36.0(7.0,36.0)months,while that of the high-expression group was 31.0(4.9,36.0)months,also showing a statistically significant difference(log rank x2=24.151,P＜0.001).Conclusion ADAM12 and S100A8 protein expression levels in triple-negative breast cancer tissues are higher than those in non-triple-negative breast cancer tissues.Serum tumor markers and ADAM12 and S100A8 protein expression levels(in both serum and tumor tissues)affect the chemotherapy outcomes and prognosis of triple-negative breast cancer patients,among which the expression levels of ADAM12 and S100A8 proteins in tumor tissues can serve as predictors of patient prognosis.

AIM:To explore the mechanism by which activation of α7 nicotinic acetylcholine receptor(nAChR)in combination with cold stimulation promotes the beiging of white adipose tissues in obese mice.METHODS:Male C57BL/6J mice,aged 8 weeks,were acclimated for 1 week before fed with high-fat diet(HFD)for 12 weeks to estab-lish an obese model.Fifty mice were randomly divided into 5 groups:control group(low-fat diet),HFD group,model group(HFD+cold stimulation),agonist group(HFD+cold stimulation+α7 nAChR agonist GTS-21),and inhibitor group(HFD+cold stimulation+α7 nAChR inhibitor α-bungarotoxin).Interventions lasted for 4 weeks.Serum levels of total cho-lesterol(TG),triglyceride(TC)and free fatty acid(FFA),and the levels of tumor necrosis factor-α(TNF-α),interleu-kin-1β(IL-1β),IL-10,Toll-like receptor 4(TLR4),nucleotide-binding oligomerization domain-like receptor protein 3(NLRP3),transforming growth factor-β(TGF-β),cyclic adenosine monophosphate(cAMP)and norepinephrine(NE)in while adipose tissues were measured using ELISA.Hematoxylin-eosin(HE)staining was performed to observe morpho-logical changes in white adipose tissues.The mRNA levels of nitric oxide synthase 2(NOS2),arginase 1(Arg1),uncou-pling protein 1(UCP1),peroxisome proliferator-activated receptor γ coactivator-1α(PGC-1α)and PR domain-containing 16(PRDM16)in white adipose tissues were determine by RT-qPCR.Immunohistochemistry was employed to examine the expression levels of CD86,CD206,C/EBP homologous protein(CHOP),immunoglobulin heavy-chain binding protein(BiP)and UCP1.Western blot analysis was conducted to detect the protein levels of CHOP,BiP,UCP1,α7 nAChR,nu-clear factor-κB(NF-κB)p65,phosphorylated Janus kinase 2(p-JAK2)and phosphorylated signal transducer and activa-tor of transcription 3(p-STAT3)in white adipose tissues.RESULTS:Activation of α7 nAChR with GTS-21,combined with cold-induced thermogenesis,led to decreased levels of TC,TG and FFA in peripheral blood and reduced lipid droplet area in white adipose tissues.Additionally,α7 nAChR activation resulted in decreased expression of TLR4 and NLRP3.In white adipose tissue,there was an increase in Arg1 mRNA level and CD206 expression,while NOS2 mRNA level and CD86 expression decreased.Phosphorylation of the JAK2/STAT3 pathway was up-regulated,and NF-κB p65 level de-creased.The levels of pro-inflammatory cytokines TNF-α and IL-1β were reduced,whereas the levels of anti-inflammatory factors TGF-β and IL-10 increased.Expression of CHOP and BiP also declined.Furthermore,α7 nAChR activation in-creased the mRNA levels of PRDM16 and PGC-1α,as well as UCP1 expression in white adipose tissues.CONCLUSION:Activation of α7 nAChR combined with cold stimulation not only promotes the beiging of white adipose tissue in obese mice,but also alliviates metabolic disorders,thereby enhancing the thermogenic efficiency of white adipose tissue beiging.

AIM:To explore the mechanism by which activation of α7 nicotinic acetylcholine receptor(nAChR)in combination with cold stimulation promotes the beiging of white adipose tissues in obese mice.METHODS:Male C57BL/6J mice,aged 8 weeks,were acclimated for 1 week before fed with high-fat diet(HFD)for 12 weeks to estab-lish an obese model.Fifty mice were randomly divided into 5 groups:control group(low-fat diet),HFD group,model group(HFD+cold stimulation),agonist group(HFD+cold stimulation+α7 nAChR agonist GTS-21),and inhibitor group(HFD+cold stimulation+α7 nAChR inhibitor α-bungarotoxin).Interventions lasted for 4 weeks.Serum levels of total cho-lesterol(TG),triglyceride(TC)and free fatty acid(FFA),and the levels of tumor necrosis factor-α(TNF-α),interleu-kin-1β(IL-1β),IL-10,Toll-like receptor 4(TLR4),nucleotide-binding oligomerization domain-like receptor protein 3(NLRP3),transforming growth factor-β(TGF-β),cyclic adenosine monophosphate(cAMP)and norepinephrine(NE)in while adipose tissues were measured using ELISA.Hematoxylin-eosin(HE)staining was performed to observe morpho-logical changes in white adipose tissues.The mRNA levels of nitric oxide synthase 2(NOS2),arginase 1(Arg1),uncou-pling protein 1(UCP1),peroxisome proliferator-activated receptor γ coactivator-1α(PGC-1α)and PR domain-containing 16(PRDM16)in white adipose tissues were determine by RT-qPCR.Immunohistochemistry was employed to examine the expression levels of CD86,CD206,C/EBP homologous protein(CHOP),immunoglobulin heavy-chain binding protein(BiP)and UCP1.Western blot analysis was conducted to detect the protein levels of CHOP,BiP,UCP1,α7 nAChR,nu-clear factor-κB(NF-κB)p65,phosphorylated Janus kinase 2(p-JAK2)and phosphorylated signal transducer and activa-tor of transcription 3(p-STAT3)in white adipose tissues.RESULTS:Activation of α7 nAChR with GTS-21,combined with cold-induced thermogenesis,led to decreased levels of TC,TG and FFA in peripheral blood and reduced lipid droplet area in white adipose tissues.Additionally,α7 nAChR activation resulted in decreased expression of TLR4 and NLRP3.In white adipose tissue,there was an increase in Arg1 mRNA level and CD206 expression,while NOS2 mRNA level and CD86 expression decreased.Phosphorylation of the JAK2/STAT3 pathway was up-regulated,and NF-κB p65 level de-creased.The levels of pro-inflammatory cytokines TNF-α and IL-1β were reduced,whereas the levels of anti-inflammatory factors TGF-β and IL-10 increased.Expression of CHOP and BiP also declined.Furthermore,α7 nAChR activation in-creased the mRNA levels of PRDM16 and PGC-1α,as well as UCP1 expression in white adipose tissues.CONCLUSION:Activation of α7 nAChR combined with cold stimulation not only promotes the beiging of white adipose tissue in obese mice,but also alliviates metabolic disorders,thereby enhancing the thermogenic efficiency of white adipose tissue beiging.

Objective To systematically evaluate the measurement properties and methodological quality of the palliative quality assessment tools based on the cancer patient experiences,and to provide an evidence-based basis for the selection of a high-quality assessment tool for healthcare professionals.Methods Systematic search of PubMed,Embase,Web of Science,CINAHL,CNKI,Wanfang database,VIP database,and China Biomedical Literature database for studies related to the evaluation of the measurement properties of the self-report palliative care quality assessment tools for cancer patients.The search period from the date of creation to December 10,2023.There are 2 researchers who independently conducted literature screening and data extraction according to the COSMIN selection criteria,and used the COSMIN Risk of Bias Inventory and Quality Criteria Scale to measure the academic characteristics and form the final recommendations.Results A total of 19 studies involving 8 assessment tools for cancer patients were included.The final 6 assessment tools were recommended at level B.2 assessment tools are recommended at level A.Conclusion The SF-QCQ-PC and the Self-Report Palliative Care Quality Assessment Tool for Terminal Cancer Patients can be provisionally recommended.However,further validation of its measurement properties is still needed.

Objective:To explore the real experience of fertility concerns in cervical cancer patients with preserved fertility function, so as to provide a basis for developing targeted nursing measures.Methods:This study was a qualitative study of descriptive phenomenology. From June 2018 to June 2021, purposive sampling was used to select 17 young cervical cancer patients who underwent radical abdominal trachelectomy or minimally invasive laparoscopic radical trachelectomy at Zhejiang Cancer Hospital as the study subjects. Face to face semi-structured interview was conducted with patients. Colaizzi 7-step analysis method was used to analyze data and extract themes.Results:Four themes were extracted, namely the multiple manifestations caused by fertility concerns, the sense of guilt caused by diseases and fertility issues, the need for health support, and strategies to actively address psychological issues caused by fertility concerns.Conclusions:Cervical cancer patients who preserve their fertility function generally have fertility concerns and an urgent need for health support, requiring support from medical and nursing staff, families, and society. Medical and nursing staff should pay attention to the fertility concerns of such patients, provide personalized psychological intervention as soon as possible to alleviate their fertility concerns.

Stigma is common in patients with head and neck cancer, which greatly affects their physical and mental health and the quality of life. This article summarizes the research progress of stigma in patients with head and neck cancer from the aspects of research status, measurement tools, influencing factors and intervention strategies, in order to provide a reference for carrying out the research on stigma in patients with head and neck cancer in China.

Objective:To explore the application effect of non-vomiting information management system in chemotherapy-induced nausea and vomiting (CINV) of cancer patients.Methods:Using the convenient sampling method, from September 2015 to December 2018, a total of 1 980 tumor patients who received chemotherapy (including intravenous chemotherapy and intravenous plus oral chemotherapy) in Department of Abdominal and Breast Oncology in Zhejiang Cancer Hospital were selected as the pre-improvement group (before the application of non-vomiting information management system) . The 332 patients who received chemotherapy from October to December 2019 were selected as the post-improvement group (after the application of non-vomiting information management system) . From May 2019, the non-vomiting information management system was constructed to form the clinical pathways, including the risk assessment module of CINV, the medical order issuance module for anti-vomiting and the follow-up system module, and the system began trial operation in October 2019. The CINV detection status, nurse satisfaction, patient satisfaction and other indexes were compared between the two groups before and after the improvement.Results:After the application of non-vomiting information management system, the detection rate of CINV of patients, nurse satisfaction and patient satisfaction increased, and the differences were statistically significant ( P<0.05) . Conclusions:The application of non-vomiting information management system is helpful to dynamically understand the occurrence of CINV in patients anytime and anywhere, give timely guidance and treatment and improve the satisfaction of nurses and patients.

Objective: To investigate the current status and influencing factors of basis of the replacement of short peripheral intravenous catheters for clinical nurses and explore the obstruction of the latest guide promoted in practice. Methods: The qualitative research method was adopted, and semistructured interviews were used to interview 11 clinical nurses. Data were analyzed by the method of category analysis. Results: Although the clinical nurses mainly used the national standards to do practical operation, they preferred to carry out catheter replacement according to clinical indications. The difference between the latest guide and the national standards was the main obstruction in the practical promotion of the latest guide. Conclusions The removal of short peripheral intravenous catheters according to clinical indications is more suitable for clinical practice. Further improving the standards of intravenous therapy in China has become a fundamental measure to solve the contradictions of work of clinical nurses, improve patients′ medical experience and save medical resources.

Objective: To explore the statue quo of psychological resilience of breast cancer patients during chemotherapy period and its influencing factors. Methods: From December 12 to May 2018, Totally 165 breast cancer patients during chemotherapy period in Department of Mammary Chemotherapy of Zhejiang Cancer Hospital were investigated by self-designed demographic questionnaire, the Connor-Davidson Resilience Scale and the Medical Coping Modes Questionnaire. Results: The total score of psychological resilience for breast cancer patients during chemotherapy period was (60.26±16.17)points.Multiple regression analysis showed that marital status, transfer, confront and resignation coping style were the influencing factors for psychological resilience(t=-3.048, 3.216, 3.070, -3.044, all P<0.05). Conclusion The level of psychological resilience of breast cancer patients is poor and multivariate analysis appears that marital status, transfer, confront and resignation coping style are important influencing factors.

Objective To summarize the experience of nursing six patients who showed pitch-off syndrome when the port was implanted and to analyze the causes of pitch-off syndrome so as to guide the nursing and observation of patients who received port implantation.Methods By observing the patients' symptoms and physical signs based on X-ray reports, pitch-off syndrome was diagnosed. Then correct treatment methods were chosen according to the severity of pitch-off syndrome.Results The six patients with pitch-off syndrome were treated based on their severity. The port-cath was removed in two patients since it cracked. The port-cath was removed in two patients who showed level one pitch-off syndrome after two cycles of chemotherapy. And the port-cath of the remaining two patients was also removed to prevent it from cracking since their level one pitch-off worsened to level three.Conclusions Pitch-off syndrome is the most dangerous complication caused by port implantation. Nursing staff should promptly assess and identify the risks of pitch-off syndrome. The port-cath should be used with great caution when the patients suffer from pitch-off syndrome but badly need venous access. In the event of in vivo cath cracks, nursing staff should work together with doctors to remove the port immediately. Pitch-off syndrome prevention is an important measure to reduce cath cracks.