Objective This study aims to establish a method for setting standardized cost ratio target values for inpatient departments,providing practical cases and theoretical support for effective implementation of DRG management.Methods Based on the 2022 Guangdong Province DRG big data,the standardized cost ratios for each department in a hospital in 2023 were calculated.By comparing the discharge cost ratio,departmental standardized cost ratio,and traditional target management cost ratio,combined with hospital control objectives,the cost ratio target values for each inpatient department were determined.Re-sults Through variance homogeneity test,significant differences were found among the three sets of cost ratio data.Based on the difference between the discharge cost ratio and the departmental standardized cost ratio,the departments were classified as lag-ging,saturated,or growing types for management control.From January to March 2024,the inpatient department cost ratio was 2.58 percentage points lower than the standardized cost ratio for the province.The monitoring of admission and discharge disease structure before and after was statistically analyzed,and it showed statistical significance when divided into three categories:RW＜1,1 ≤RW≤2,RW＞2.Based on disease-specific standardized cost ratio management,the acceptance rate of 48 inpatient de-partments was 83.33％.Conclusion The research on setting inpatient department cost ratio target values based on Diagnosis-Related Groups(DRG)is a measure for hospital refined management.It is scientific and reasonable,conducive to further optimi-zing the structure of medical income,and improving operational efficiency.

Purpose To investigate the expression of bone sialoprotein(BSP)in the tissues and cells of endome-trial cancer(EC)and its effects on the proliferation and invasion of EC cells.Methods The expression of BSP was assessed by immunohistochemistry in 235 EC tissues and 88 normal endometrial tissues,and its correlation with clinico-pathological features was analyzed.Western blot was used to compare BSP levels between human endometrial carcinoma cell line(HHUA)and normal human endumetial epithelial cells(HEEC).BSP was knocked down in HHUA cells via transient transfection,and the cells were divided into blank control group and BSP-knockdown group.The effects of BSP knockdown on cell cycle,proliferation,migration,invasion,and apoptosis were evaluated using PI staining,CCK-8,scratch,Transwell,and Annexin V-FITC assays,respectively.Protein levels of TGF-β signaling pathway compo-nents were analyzed by Western blot.Results BSP expression was significantly higher in EC tissues than in normal endometrium(P＜0.001)and correlated with lymph node metastasis and advanced FIGO stage(P＜0.05).BSP pro-tein level was also significantly elevated in HHUA cells(2.455 8±0.008 9)compared to HEECs(1.571 2±0.005 4)(P＜0.01).After knockdown,compared with the control group,the proliferation index(74.4±3.33),migration rate(0.48±0.03),and invasion ability(0.36±0.11)of the cells were increased,and the apoptosis rate(25.97％)of the cells was increased(P＜0.05).Furthermore,the expression levels of TGF-β signaling pathway downstream proteins TGF-β1(0.290 4±0.002 3)、TGF-β2(0.292 9±0.001 6)、Smad2(0.469 3±0.001 1)、Smad3(0.247 0±0.001 7)、pAKT(0.382 1±0.001 9)、ATK(0.119 6±0.001 6)and MEK1(0.258 9±0.000 3)in the BSP-knockdown group of EC cells decreased(P＜0.01).Conclusion BSP is highly expressed in endometrial cancer and promotes cancer cell proliferation,invasion,and metastasis by activating the TGF-β signaling pathway.

Purpose To investigate the expression of bone sialoprotein(BSP)in the tissues and cells of endome-trial cancer(EC)and its effects on the proliferation and invasion of EC cells.Methods The expression of BSP was assessed by immunohistochemistry in 235 EC tissues and 88 normal endometrial tissues,and its correlation with clinico-pathological features was analyzed.Western blot was used to compare BSP levels between human endometrial carcinoma cell line(HHUA)and normal human endumetial epithelial cells(HEEC).BSP was knocked down in HHUA cells via transient transfection,and the cells were divided into blank control group and BSP-knockdown group.The effects of BSP knockdown on cell cycle,proliferation,migration,invasion,and apoptosis were evaluated using PI staining,CCK-8,scratch,Transwell,and Annexin V-FITC assays,respectively.Protein levels of TGF-β signaling pathway compo-nents were analyzed by Western blot.Results BSP expression was significantly higher in EC tissues than in normal endometrium(P＜0.001)and correlated with lymph node metastasis and advanced FIGO stage(P＜0.05).BSP pro-tein level was also significantly elevated in HHUA cells(2.455 8±0.008 9)compared to HEECs(1.571 2±0.005 4)(P＜0.01).After knockdown,compared with the control group,the proliferation index(74.4±3.33),migration rate(0.48±0.03),and invasion ability(0.36±0.11)of the cells were increased,and the apoptosis rate(25.97％)of the cells was increased(P＜0.05).Furthermore,the expression levels of TGF-β signaling pathway downstream proteins TGF-β1(0.290 4±0.002 3)、TGF-β2(0.292 9±0.001 6)、Smad2(0.469 3±0.001 1)、Smad3(0.247 0±0.001 7)、pAKT(0.382 1±0.001 9)、ATK(0.119 6±0.001 6)and MEK1(0.258 9±0.000 3)in the BSP-knockdown group of EC cells decreased(P＜0.01).Conclusion BSP is highly expressed in endometrial cancer and promotes cancer cell proliferation,invasion,and metastasis by activating the TGF-β signaling pathway.

Objective This study aims to establish a method for setting standardized cost ratio target values for inpatient departments,providing practical cases and theoretical support for effective implementation of DRG management.Methods Based on the 2022 Guangdong Province DRG big data,the standardized cost ratios for each department in a hospital in 2023 were calculated.By comparing the discharge cost ratio,departmental standardized cost ratio,and traditional target management cost ratio,combined with hospital control objectives,the cost ratio target values for each inpatient department were determined.Re-sults Through variance homogeneity test,significant differences were found among the three sets of cost ratio data.Based on the difference between the discharge cost ratio and the departmental standardized cost ratio,the departments were classified as lag-ging,saturated,or growing types for management control.From January to March 2024,the inpatient department cost ratio was 2.58 percentage points lower than the standardized cost ratio for the province.The monitoring of admission and discharge disease structure before and after was statistically analyzed,and it showed statistical significance when divided into three categories:RW＜1,1 ≤RW≤2,RW＞2.Based on disease-specific standardized cost ratio management,the acceptance rate of 48 inpatient de-partments was 83.33％.Conclusion The research on setting inpatient department cost ratio target values based on Diagnosis-Related Groups(DRG)is a measure for hospital refined management.It is scientific and reasonable,conducive to further optimi-zing the structure of medical income,and improving operational efficiency.

Objective To investigate the risk of atopic disease in infants with a atopic mothers. Methods The level of CCL22 and total IgE in the cord blood were measured using ELISA for 33 newborns with atopic mothers and for 44 newborns with non-atopic mothers. Correlation between the two factors was examined. Periodic follow-ups were conducted on the newborns to observe the risk of atopic diseases. Results The atopic group showed a higher level of CCL22 than that in non-atopic group, and the difference was statistically significant (Z=5.20, P=0.000). When 0.9 kU/L was taken as the threshold of an elevated IgE level in cord blood, the positive rates of the atopic group (11/33) was much higher than that of the non-atopic group (4/44) (χ2=7.07, P=0.008). Furthermore, the level of CCL22 and the level of IgE were significantly positively correlated (r=0.808, P=0.000; r=0.348, P=0.021) in the atopic group and the non-atopic group, respectively. During the 12 months of follow-up, the number of atopic diseases occurred in the infants in the atopic group (24/33) was much higher than that in the non-atopic group (10/44) (χ2=19.12, P<0.001).Significant correlation exists between levels CCL22 and total IgE in cord blood and infant atopic diseases (Z=5.36, P=0.000; Z=4.44, P=0.000). Conclusions At birth, the infants with an atopic mother are already in a sensitization state and have a tendency to develop potential atopic diseases. There is a correlation between the history of atopic diseases in the mothers and the elevated level of CCL22 in the cord blood of the newborns, and the probability of developing atopic diseases for the newborns is significantly higher when the level of CCL22 is elevated. The combined detection of CCL22 and IgE levels impact significantly on the prediction of the risk of atopic diseases clinically.