Objective:To establish a mouse model of spinal cord injury(SCI),and to investigate the effect of electroacupuncture(EA)intervention on cell apoptosis after acute SCI and its mechanism.Methods:Female C57BL/6 mice were used to establish a model of SCI,and after successful modeling,the mice were randomly divided into SCI group,EA group,and Rosiglitazone group(R group);a sham-operation group(Sham group)was also established.After successful modeling,the mice in the EA group were given EA at bilat-eral Jiaji points and Zusanli once a day for 14 days,those in the R ture,and the number of surviving cells.The EA group and the R group had a significant reduction in the expression of caspase-3 and significant increases in the expression of PPARγ and CD36,and the EA group had significant reductions in the expression of Hba-a1 and Hbb-bt.In addition,RNA-Seq and TMT/iTRAQ techniques,significant analysis,Venn analysis,and dual-omics analysis identi-fied Hba-a1 and Hbb-bt as the target genes of EA.The KEGG pathway enrichment analysis showed that EA had a significant effect on the PPAR signaling pathway.Conclusion:By regulating the PPARγ-CD36 signaling pathway,EA can promote the clearance of Hba-a1 and Hbb-bt after SCI,reduce the expression level of caspase-3,alleviate cell apoptosis,and facilitate the recovery of spinal cord nerve function.

OBJECTIVE: Positively related to functional connectivity using resting state fMRI functional connectivity method to observe the right of sudden deafness of auditory cortex in patients with brain. METHOD: We selected the right side of the 12 cases of patients with sudden deafness resting state fMRI data acquisition, positive correlation function for the observation about the right of sudden deafness patients using the method of functional connectivity brain auditory cortex and the brain regions to connect brain map, and matched the normal hearing group the difference. RESULT: The right side of sudden deafness in patients with valid data for the seed point of A I bilateral. The brain was activated network included bilateral transverse gyrus, superior temporal gyrus, insula, cingulate gyrus and supplementary motor area. Brain networks were activated like a normal person, but there were differences between the two. CONCLUSION The right side of the deafness of A I seed point the functional connectivity of the auditory system is still mainly confined to the auditory system, but the local auditory cortex functional reorganization occurs.
Adult ; Auditory Cortex ; physiopathology ; Case-Control Studies ; Female ; Hearing Loss, Sudden ; pathology ; physiopathology ; Humans ; Magnetic Resonance Imaging ; methods ; Male ; Middle Aged