Transmembrane protein 33(TMEM33)is up-regulated in the majority of cancers.To change the expression of TMEM33 can significantly affect the proliferation,migration,and invasion of renal and cervical cancer cells.Its mechanisms of action may involve four aspects:1)participation in lipid metabolism;2)regu-lation of endoplasmic reticulum;3)regulation of intracellular calcium homeostasis;4)participation in angio-genesis.

Objective To investigate the impact of DNA damage-regulated autophagy factor 2(DRAM2)on the proliferation and migration of non-small cell lung cancer(NSCLC)cells through tumor protein p53(p53)and autophagy.Methods DRAM2 gene was knocked down using lentiviral technology to establish NSCLC cell lines,and autophagy markers were detected by immunofluorescence and Western blot.CCK8 and Transwell assays were used to detect cell proliferation and migration.The effects of autophagy activation and p53 knockdown on autoph-agy and functions of DRAM2?knockdown cells were examined.Results Knockdown of DRAM2 inhibited NSCLC cells,with upregulation of p62 expression(P<0.05)and decreased level of LC3?Ⅱ(P<0.05).Knockdown of DRAM2 suppressed the proliferation(P<0.001)and migration(P<0.001)of NSCLC cells.Activation of auto?phagy partially reversed the inhibitory effects of DRAM2 knockdown on cell proliferation(P<0.01)and migration(P<0.01).When DRAM2 and p53 were knocked down simultaneously,autophagy,cell proliferation(P<0.05)and migration abilities(P<0.001)were restored.Conclusions Knockdown of DRAM2 inhibits the proliferation and migration of lung cancer cell line A549,providing a potential intervention direction for the devel?opment of therapeutic strategies.

Objective To examine the level of sperm flagellar tubulin glycosylation(Gly-Tubulin)in asthenospermia patients(AP)and discuss its significance.Methods According to the principle of inclusion and exclusion criteria,108 patients treated in the Department of Andrology in Xinjiang Uygur Autonomous Region Maternal and Child Health Hospital from June 2021 to May 2022 were rolled in this study.According to the results of semen examine,they were divided into group AP(n=74),and normal control(NC)group(n=34).Colleced the clinical date of both group,such as the age,the sterility period.The levels of sex hormones were detected by chemiluminescence method.The glycosylated Tubulin levels of the sperm were detected by Western blot and immunofluorescence was used for localization analysis,and the relationship between the Gly-Tubulin of sperm flagellar and asthenospermia was analyzed by Multivariate Logistic regression analysis.Results Compared to NC group,the sterility period of group AP was prolong(3.98±0.30 vs 0.78±0.08),the percent of forward sperm motility was decreased(18.55±0.97 vs 46.24±1.89)and the expression level of Gly-Tubulin in the sperm flagellar was decreased(0.64±0.11 vs 1.00±0.00),the differences with statistical significance(t=62.038,χ2=14.394,3.148,all P＜0.05).There were no significant differences in follicle-stimulating hormone(FSH),luteinizing hormone(LH),prolactin(PRL),estradiol(E2),testosterone,semen volume,sperm concentration and total sperm between the two groups(t/χ2=0.043～1.503,all P>0.05).Multivariate Logistic regression analysis showed that the percent of forward sperm motility and the sperm flagellar Gly-Tubulin expression level were independent risk factors for male asthenospermia(Wald χ2=4.406,7.492,P<0.05).Conclusion Abnormal level of Gly Tubulin in sperm flagella may be closely related to the occurrence and development of asthenospermia.

Objective To examine the level of sperm flagellar tubulin glycosylation(Gly-Tubulin)in asthenospermia patients(AP)and discuss its significance.Methods According to the principle of inclusion and exclusion criteria,108 patients treated in the Department of Andrology in Xinjiang Uygur Autonomous Region Maternal and Child Health Hospital from June 2021 to May 2022 were rolled in this study.According to the results of semen examine,they were divided into group AP(n=74),and normal control(NC)group(n=34).Colleced the clinical date of both group,such as the age,the sterility period.The levels of sex hormones were detected by chemiluminescence method.The glycosylated Tubulin levels of the sperm were detected by Western blot and immunofluorescence was used for localization analysis,and the relationship between the Gly-Tubulin of sperm flagellar and asthenospermia was analyzed by Multivariate Logistic regression analysis.Results Compared to NC group,the sterility period of group AP was prolong(3.98±0.30 vs 0.78±0.08),the percent of forward sperm motility was decreased(18.55±0.97 vs 46.24±1.89)and the expression level of Gly-Tubulin in the sperm flagellar was decreased(0.64±0.11 vs 1.00±0.00),the differences with statistical significance(t=62.038,χ2=14.394,3.148,all P＜0.05).There were no significant differences in follicle-stimulating hormone(FSH),luteinizing hormone(LH),prolactin(PRL),estradiol(E2),testosterone,semen volume,sperm concentration and total sperm between the two groups(t/χ2=0.043～1.503,all P>0.05).Multivariate Logistic regression analysis showed that the percent of forward sperm motility and the sperm flagellar Gly-Tubulin expression level were independent risk factors for male asthenospermia(Wald χ2=4.406,7.492,P<0.05).Conclusion Abnormal level of Gly Tubulin in sperm flagella may be closely related to the occurrence and development of asthenospermia.

Objective To investigate the impact of DNA damage-regulated autophagy factor 2(DRAM2)on the proliferation and migration of non-small cell lung cancer(NSCLC)cells through tumor protein p53(p53)and autophagy.Methods DRAM2 gene was knocked down using lentiviral technology to establish NSCLC cell lines,and autophagy markers were detected by immunofluorescence and Western blot.CCK8 and Transwell assays were used to detect cell proliferation and migration.The effects of autophagy activation and p53 knockdown on autoph-agy and functions of DRAM2?knockdown cells were examined.Results Knockdown of DRAM2 inhibited NSCLC cells,with upregulation of p62 expression(P<0.05)and decreased level of LC3?Ⅱ(P<0.05).Knockdown of DRAM2 suppressed the proliferation(P<0.001)and migration(P<0.001)of NSCLC cells.Activation of auto?phagy partially reversed the inhibitory effects of DRAM2 knockdown on cell proliferation(P<0.01)and migration(P<0.01).When DRAM2 and p53 were knocked down simultaneously,autophagy,cell proliferation(P<0.05)and migration abilities(P<0.001)were restored.Conclusions Knockdown of DRAM2 inhibits the proliferation and migration of lung cancer cell line A549,providing a potential intervention direction for the devel?opment of therapeutic strategies.