Tensile stimulation plays a crucial role in regulating tissue structure and function.Due to the limitations of in vivo studies,engineered tension tissues(ETTs)based on biomaterials and tissue engineering technologies have gradually become a research hotspot.Specifically,microscale-engineered tension tissues(μETTs)based on flexible microstructures overcome many limitations of traditional ETTs,due to their controllable mechanical constraints and precise mechanical characterization and stimulation capabilities.This has led to their widespread applications in disease research,drug screening,and toxicologic studies.This review summarizes the materials and fabrication method of flexible microstructures,analyzes their biomechanical roles in μETTs,provides an overview of research progress in myocardial,lung,and skeletal muscle μETTs,discusses mechanical modeling and analysis methods during the remodeling of μETTs,and looks ahead to the future development of this field.

Tensile stimulation plays a crucial role in regulating tissue structure and function.Due to the limitations of in vivo studies,engineered tension tissues(ETTs)based on biomaterials and tissue engineering technologies have gradually become a research hotspot.Specifically,microscale-engineered tension tissues(μETTs)based on flexible microstructures overcome many limitations of traditional ETTs,due to their controllable mechanical constraints and precise mechanical characterization and stimulation capabilities.This has led to their widespread applications in disease research,drug screening,and toxicologic studies.This review summarizes the materials and fabrication method of flexible microstructures,analyzes their biomechanical roles in μETTs,provides an overview of research progress in myocardial,lung,and skeletal muscle μETTs,discusses mechanical modeling and analysis methods during the remodeling of μETTs,and looks ahead to the future development of this field.

ObjectiveTo analyze the expression of Lactate dehydrogenase A（LDHA） in both renal cell carcinoma （RCC） tissue and RCC cell lines， and to investigate the impact of LDHA expression on the progression of RCC. MethodsFrom June 2018 to June 2022， totally 52 cases of RCC tissue samples and 49 cases of para-cancerous tissue samples were collected through surgical procedures from our hospital. LDHA expression was detected using immunohistochemistry （IHC）. The expression levels of LDHA in vitro were also detected in the normal human proximal tubule epithelial cell line HK-2 and renal cell carcinoma cell lines A498， Caki-2， ACHN， and 786-O by using qRT-PCR and Western blot. A recombinant plasmid carrying LDHA-shRNA was constructed and then transfected into 786-O cells to down-regulate the expression of LDHA. Tumor proliferative capacity was monitored using CCK-8 assay， clonal formation assay and EdU assessments. Additionally， cell glycolytic activity was assessed through glucose uptake assay， lactate secretion assay， and ECAR analysis. ResultsIHC analysis revealed significantly higher expression of LDHA in RCC tissue compared to adjacent tissues（P<0.05）. Furthermore， RCC tissues with higher TNM stage exhibited greater expression of LDHA than those with lower TNM stage （P<0.05）. The results of qRT-PCR and Western blot demonstrated that the expression of LDHA in each RCC cell line was significantly higher than that in HK-2（P<0.05）. After blocking the expression of LDHA in 786-O， there was a significant down-regulation of cell proliferation and glycolysis capacity （P<0.05）. ConclusionsThe expression of LDHA in RCC tissue and RCC cell lines is significantly overexpressed compared with normal one， particularly in those with higher TNM stage. Knockdown of the expression of LDHA significantly suppresses cell proliferation and aerobic glycolysis capacity in 786-O.

We tried to find the effects of the application of the antibacterial solution containing silver ions on the surface of the denture soft lining material. We selected the right concentration of the silver-containing solution and coated a soft lining material with the solution so that the soft lining material could be antibacterial. The antibacterial solution containing silver ions was prepared by sol-gel method. MIC of C. a and S. a were tested by broth dilution test. The surface property and thickness were tested after coated. The in vitro antibacterial ratio against C. a and S. a were demonstrated by the method of plate-counting. A film was formed after coating, while the adequacy was not changed. Antibacterial ratio of 0.64 mg/ml group against C. a was 90.82%, and that against S. a was 94.96% in 24 hours, respectively. It was found that the antibacterial property of the soft lining material can be acquired by coating this antibacterial solution with silver ion, without changing the adequacy.
Anti-Bacterial Agents ; chemistry ; pharmacology ; Candida albicans ; drug effects ; Denture Cleansers ; pharmacology ; Denture Liners ; microbiology ; Membranes, Artificial ; Microbial Sensitivity Tests ; Silver ; pharmacology ; Staphylococcus aureus ; drug effects