Dental implant surgery,an established practice,has been widely used in patients with dentition defect.Dental implanta-tion-induced osteomyelitis of the jaw is a rare complication.Osteomyelitis of the jaw is an inflammatory change of the jaw caused by bac-terial infection or other physical and chemical factors involving the whole bone tissue,such as periosteum,blood vessels and nerves in the medullary cavity,etc.Due to protracted course and complicated treatment of the disease,the consequences will be serious if not swiftly managed.Here,an unusual case of severe mandibular osteomyelitis following mandibular implant surgery is reported.Also,re-lated literature is reviewed to elucidate the clinical characteristics and management as to highlight the understanding of this complication and provide reference for clinical prevention,diagnosis and treatment.

Dental implant surgery,an established practice,has been widely used in patients with dentition defect.Dental implanta-tion-induced osteomyelitis of the jaw is a rare complication.Osteomyelitis of the jaw is an inflammatory change of the jaw caused by bac-terial infection or other physical and chemical factors involving the whole bone tissue,such as periosteum,blood vessels and nerves in the medullary cavity,etc.Due to protracted course and complicated treatment of the disease,the consequences will be serious if not swiftly managed.Here,an unusual case of severe mandibular osteomyelitis following mandibular implant surgery is reported.Also,re-lated literature is reviewed to elucidate the clinical characteristics and management as to highlight the understanding of this complication and provide reference for clinical prevention,diagnosis and treatment.

OBJECTIVETo investigate the effect of cyclic stretch on p38 mitogen-activated protein kinase (p38MAPK) signaling pathway during stretch-induced differentiation process of C2C12 myoblasts.

METHODSC2C12 cells were seeded on Bio Flex 6-well plates, and cells were subsequently subjected to cyclic stretch at an optimal magnitude (10%) and frequency (0.5 Hz). The effects of cyclic stretch were examined at 2, 6, 12, 24 h. Antibodies specific to p38MAPK phosphorylated forms and the total protein levels of the p38MAPK were examined using Western blot analysis.

RESULTSThese results indicated that p38MAPK was activated during stretch-induced C2C12 cell differentiation. The level of phosphorylated protein was higher in the p38MAPK signaling pathway. The expression of total protein was maintained at baseline level. There were no significant differences between groups. Treatment of cells with specific p38MAPK inhibitor SB203580 could decrease the expression of myogenin, but not completely abolish the myogenin expression after stretch.

CONCLUSIONp38MAPK signaling pathway plays an important role during stretch-induced differentiation process of C2C12 myoblasts, but is not activated exclusively in this process.

Cell Differentiation ; Humans ; Imidazoles ; Myoblasts ; Phosphorylation ; Pyridines ; Signal Transduction ; p38 Mitogen-Activated Protein Kinases