ObjectiveTo explore the possible mechanism of Jianpi Huazhuo Tiaozhi Granule （健脾化浊调脂颗粒， JHTG） in treating atherosclerosis （AS） based on the regulation of perivascular adipose tissue （PVAT） browning via the phosphoinositide 3-kinase/protein kinase B （PI3K/AKT） signaling pathway. MethodsFifteen SPF male C57BL/6J mice served as control group， while 76 ApoE^-/- mice were first fed a high-fat diet for 16 weeks to establish AS model. After successful modeling， they were randomly divided into model group （n=16）， as well as the browning group， the low-， medium- and high-dose JHTG group， with 15 mice in each group. The browning group was intraperitoneally injected with β3-adrenergic receptor agonist CL 316243 at 1 mg/（kg·d） once daily. The low-， medium- and high-dose JHTG groups were treated with 4.3 g/（kg·d）， 8.6 g/（kg·d）， and 17.2 g/（kg·d） of JHTG by gavage， respectively， while the control group and the model group were given normal saline at 10 ml/（kg·d） by intragastric administration， once daily. All groups received continuous intervention for 4 weeks. The aorta was collected to assess the plaque ratio by gross oil red O staining. HE staining was used to measure the plaque area in cross-sections. Masson staining was employed to detect the proportion of collagen fibers in plaque. Transmission electron microscopy was used to observe the quantity and morphological changes of mitochondria and lipid droplets in adipocytes. The levels of serum triglycerides （TG）， total cholesterol （TC）， high-density lipoprotein cholesterol （HDL-C）， and low-density lipoprotein cholesterol （LDL-C） were detected， as well as tumor necrosis factor α （TNF-α） and interleukin 6 （IL-6） levels in the PVAT and aorta. The protein levels of adiponectin and leptin in PVAT， and protein expression levels of phosphoinositide 3-kinase （PI3K）， phosphorylated phosphoinositide 3-kinase （p-PI3K）， protein kinase B （AKT）， phosphorylated protein kinase B （p-AKT）， uncoupling protein 1 （UCP1）， peroxisome proliferator-activated receptor γ coactivator 1α （PGC-1α）， and PR domain containing 16 （PRDM16） were measured. The mRNA expression levels of UCP1， PGC-1α and PRDM16 in PVAT were detected. ResultsCompared to the control group， the model group showed increased serum TG， TC， and LDL-C levels and decreased HDL-C level， elevated TNF-α and IL-6 levels in PVAT and aorta， decreased average fluorescence intensity of adiponectin， increased average fluorescence intensity of leptin， reduced p-PI3K/PI3K and p-AKT/AKT values as well as protein levels and mRNA expression levels of UCP1， PGC-1α and PRDM16 （P＜0.01）. The pathological results showed that no significant plaque formation was observed in the aortas of mice in the control group. In the model group， multiple plaques were observed in the aortas， with large numbers of foam cells， cholesterol crystals， and inflammatory cell aggregation in the plaques. Compared to the model group， the browning group and the high-dose JHTG group significantly improved the above indicators and aortic pathological changes （P＜0.05 or P＜0.01）. Three JHTG groups showed a dose-dependent effect in reducing LDL-C level and plaque ratio by gross oil red O staining， elevating the average fluorescence intensity of adiponectin， the p-AKT/AKT value， the protein level of PGC-1α， and the mRNA expression levels of UCP1 and PRDM16 （P＜0.05）. The high-dose JHTG group and the browning group showed similar efficacy in improving the pathology of the aorta. ConclusionJHTG may promote PVAT browning in AS model mice through the PI3K/AKT pathway， improve the endocrine function of PVAT， inhibit aortic inflammation， and thereby ameliorate the formation of AS plaques.

Neurofibromatosis type 1 (NF1) presents with a diverse range of symptoms that can affect the skin, bones, eyes, central nervous system, and other organs. This article reports the diagnosis and treatment process of a patient with NF1 complicated by bilateral severe-to-profound sensorineural hearing loss. Genetic testing revealed a heterozygous variant of NF1 NM_ 000267.3: c.4054_ 4058del(p.Ser1352LeufsTer20, supporting the diagnosis of NF1. After thorough evaluation, a right cochlear implantation was performed, yielding satisfactory postoperative results. This case suggests that NF1 patients may exhibit phenotypic heterogeneity and atypicality, providing a reference for clinicians in the diagnosis and treatment of such patients.

Objective:To compare the significantly differentially expressed proteins in peripheral blood mononuclear cells between patients with different types of depressive disorders and normal participants and explore objective biomarkers with diagnostic significance.Methods:From March to May 2024, peripheral blood samples were collected from bipolar depression (BP), simple depression (DEP), persistent depressive disorder (PDD), and normal participants (NP) at the outpatient department of the Mental Health Center of Ningxia Medical University General Hospital. Using data-independent acquisition (DIA) quantitative proteomics technology, differentially expressed proteins were identified in the peripheral blood of patients with different types of depressive disorders and normal participants, conducting bioinformatics analysis to obtain candidate objective biomarkers, and finally identifying common and unique objective biomarkers for different types of depressive disorders. Data analysis was conducted using R software (version 4.0) and SPSS 27.0 software.Results:A total of 167 differentially expressed proteins were screened between BP and NP, 30 differentially expressed proteins were screened between DEP and NP, and 14 differentially expressed proteins were screened between PDD and NP.The differential proteins unique to the BP group were ARPC4 SPARC、MTPN、SNCA、YWHAH、MIF、COTL1, the differentially expressed proteins unique to the DEP group were RBBP9 APOF、S100A6, and the unique differential proteins in the PDD group were APOA5 CA1、IGLV3-25、APOC2.Conclusion:The objective biomarkers and signaling pathways obtained using DIA quantitative proteomics technology are of great significance for revealing the biological basis and diagnosing accurately of different types of depressive disorders.

miR-101-3p is an evolutionarily conserved non-coding RNA that has shown aberrant expression and regulatory functios in various cardiovascular diseases(CVDs)in recent years.It participates in key pathological processes including post-infarction myocardial remodeling,myocardial fibrosis in heart failure and the development of atherosclerosis by modulating pathways such as apoptosis,autophagy and metabolism.In addition,it exhibits immunoregulatory potential in myocardium injury related to systemic diseases.This review summarizes the recent advances in understanding the role of miR-101-3p in CVDs,focusing on its expression dynamics and regulatory mechanisms in different disease models.It further elaborates on the biological effects of miR-101-3p in cardiovascular pathology and explores its potential as a diagnostic biomarker and therapeutic target.miR-101-3p is expected to provide novel insights for early detection and targeted intervention of cardiovascular diseases.

miR-101-3p is an evolutionarily conserved non-coding RNA that has shown aberrant expression and regulatory functios in various cardiovascular diseases(CVDs)in recent years.It participates in key pathological processes including post-infarction myocardial remodeling,myocardial fibrosis in heart failure and the development of atherosclerosis by modulating pathways such as apoptosis,autophagy and metabolism.In addition,it exhibits immunoregulatory potential in myocardium injury related to systemic diseases.This review summarizes the recent advances in understanding the role of miR-101-3p in CVDs,focusing on its expression dynamics and regulatory mechanisms in different disease models.It further elaborates on the biological effects of miR-101-3p in cardiovascular pathology and explores its potential as a diagnostic biomarker and therapeutic target.miR-101-3p is expected to provide novel insights for early detection and targeted intervention of cardiovascular diseases.

Objective:To compare the significantly differentially expressed proteins in peripheral blood mononuclear cells between patients with different types of depressive disorders and normal participants and explore objective biomarkers with diagnostic significance.Methods:From March to May 2024, peripheral blood samples were collected from bipolar depression (BP), simple depression (DEP), persistent depressive disorder (PDD), and normal participants (NP) at the outpatient department of the Mental Health Center of Ningxia Medical University General Hospital. Using data-independent acquisition (DIA) quantitative proteomics technology, differentially expressed proteins were identified in the peripheral blood of patients with different types of depressive disorders and normal participants, conducting bioinformatics analysis to obtain candidate objective biomarkers, and finally identifying common and unique objective biomarkers for different types of depressive disorders. Data analysis was conducted using R software (version 4.0) and SPSS 27.0 software.Results:A total of 167 differentially expressed proteins were screened between BP and NP, 30 differentially expressed proteins were screened between DEP and NP, and 14 differentially expressed proteins were screened between PDD and NP.The differential proteins unique to the BP group were ARPC4 SPARC、MTPN、SNCA、YWHAH、MIF、COTL1, the differentially expressed proteins unique to the DEP group were RBBP9 APOF、S100A6, and the unique differential proteins in the PDD group were APOA5 CA1、IGLV3-25、APOC2.Conclusion:The objective biomarkers and signaling pathways obtained using DIA quantitative proteomics technology are of great significance for revealing the biological basis and diagnosing accurately of different types of depressive disorders.

Objective To explore the effects of the regulation of JAK/STAT signaling pathway by the therapy of astringent ulcer and muscle inducing enema on colon injury and mucosal barrier in ulcerative colitis(UC)model rats.Methods A rat model of ulcerative colitis was established using 2,4,6-trinitrobenzenesulfonic acid/ethanol method.The model was randomly divided into a normal control group(without modeling),a model group,a positive drug group(mesalazine sustained-release granules 0.42 g·kg-1),a high dose of Lianchuang Shengji Formula(enema 10.5 g·kg-1),a medium dose(enema 5.25 g·kg-1),and a low dose group(enema 2.62 g·kg-1).Compare the fecal occult blood,degree of colonic mucosal damage,and pathological changes of rats in each group,and detect the expression of JAK2,STAT3 proteins,and their phosphorylated proteins in colon tissue using Western blot method.Results The results showed that the mesalazine group,the medium and high-dose groups of Lianchuang Shengji Formula could increase the body mass of rats,reduce fecal occult blood score,improve colon damage,and achieve pathological results;The mesalazine group and the low,medium,and high dose groups of Lianchuang Shengji can downregulate the expression of p-JAK2 and p-STAT3 proteins(compared with the model group,P＜0.05),with the high-dose group being the most significant(P＜0.01).The apoptosis rate of colon tissue significantly decreased(P＜0.05).Conclusion The enema therapy of astringent ulcer and promoting muscle growth can significantly improve the pathological scores of bloody stools and colon injury in rats with ulcerative colitis.The mechanism may be related to the inhibition of JAK2 and STAT3 phosphorylation protein expression,and further inhibition of the JAK/STAT signaling pathway.

Objective To explore the effects of the regulation of JAK/STAT signaling pathway by the therapy of astringent ulcer and muscle inducing enema on colon injury and mucosal barrier in ulcerative colitis(UC)model rats.Methods A rat model of ulcerative colitis was established using 2,4,6-trinitrobenzenesulfonic acid/ethanol method.The model was randomly divided into a normal control group(without modeling),a model group,a positive drug group(mesalazine sustained-release granules 0.42 g·kg-1),a high dose of Lianchuang Shengji Formula(enema 10.5 g·kg-1),a medium dose(enema 5.25 g·kg-1),and a low dose group(enema 2.62 g·kg-1).Compare the fecal occult blood,degree of colonic mucosal damage,and pathological changes of rats in each group,and detect the expression of JAK2,STAT3 proteins,and their phosphorylated proteins in colon tissue using Western blot method.Results The results showed that the mesalazine group,the medium and high-dose groups of Lianchuang Shengji Formula could increase the body mass of rats,reduce fecal occult blood score,improve colon damage,and achieve pathological results;The mesalazine group and the low,medium,and high dose groups of Lianchuang Shengji can downregulate the expression of p-JAK2 and p-STAT3 proteins(compared with the model group,P＜0.05),with the high-dose group being the most significant(P＜0.01).The apoptosis rate of colon tissue significantly decreased(P＜0.05).Conclusion The enema therapy of astringent ulcer and promoting muscle growth can significantly improve the pathological scores of bloody stools and colon injury in rats with ulcerative colitis.The mechanism may be related to the inhibition of JAK2 and STAT3 phosphorylation protein expression,and further inhibition of the JAK/STAT signaling pathway.

Objective:To study the surveillance results of soil-transmitted nematode (STN) disease at the national surveillance site in Nanchang City, so as to scientifically formulate prevention and control strategies for parasitic diseases in Nanchang City.Methods:From 2016 to 2020, in Nanchang County, the national surveillance site of STN disease in Nanchang City, no less than 1 000 fecal samples and 25 soil samples of permanent residents over 3 years of age were investigated annually. The improved Kato-Katz thick smear method was used to detect the eggs of STN in the population, species identification of hookworm was carried out by test-tube filter paper incubation, the Enterobius vermicularis eggs of 3 - 9 years old children were detected by cellophane anal swab method, and the soil samples were used to identify hookworm larvae and of Ascaris lumbricoides eggs. Results:The total infection rate of STN was 0.93% (47/5 054) from 2016 to 2020 in the national surveillance site of Nanchang City. The annual infection rates were 0.10% (1/1 000), 2.94% (30/1 021), 0.79% (8/1 008), 0.50% (5/1 002) and 0.29% (3/1 023), respectively; the infection rates of hookworm, Ascaris lumbricoides and Trichuris trichiura were 0.42% (21/5 054), 0.02% (1/5 054) and 0.49% (25/5 054), respectively. Totally 97.87% (46/47) of the infected persons were mild infection. The population distribution characteristics showed that there was significant difference in STN infection rate among different education levels (χ 2 = 18.06, P = 0.001), but there was no significant difference in infection rate among different sex, age and occupation (χ 2 = 0.92, 2.01, 13.60, P > 0.05). Among them, the infection rate of junior high school cultural was the highest (1.84%, 24/1 301). The culture of hookworm larvae showed that 90.48% (19/21) were Necator americanus; the infection rate of Enterobius vermicularis in 3 - 9 years old children, the contamination rate of soil hookworm larvae and Ascaris lumbricoides eggs, were all 0. Conclusion:STN disease shows a low prevalence trend at the national monitoring site in Nanchang City, Trichuris trichiura and hookworm are the key insect species for STN disease control in Nanchang City in the future.

Objective:To explore the relationship between parental rearing style and mental health of community correction offenders, as well as the mediating role of personality and coping styles in it.Methods:A questionnaire survey was conducted on 385 community correction offenders by the Egma Minnen av Bardndosna uppforstran(EMBU), symptom checklist-90 (SCL-90), NEO five-factor inventory(NEO-FFI) and simplified coping style questionnaire(SCSQ) from February to July 2022.SPSS 27.0 software was used for correlation analysis and regression analysis.AMOS 26.0 was used for the construction of structural equation, and Bootstrap was used for mediating effect analysis.Results:In parental rearing styles, the dimension of parents' severe punishment(19.09±5.32, 12.93±4.77), father's preference for subjects(9.29±3.30), father's excessive protection(10.40±2.19), mother's excessive interference and protection(33.81±6.06)and parents' refusal and denial (9.08±3.03, 12.17±4.25) were significantly positively correlated with the total score of SCL-90 (140.63±44.28)( r=0.114, 0.168, 0.121, 0.144, 0.224, 0.187, 0.220 respectively, all P<0.05). Parents' emotional warmth and understanding, parents' severe punishment and parents' refusal and denial were significantly correlated with coping styles ( r=0.420, 0.420, -0.189, -0.190, -0.174, -0.163 respectively, P<0.05). Neuroticism, extraversion, conformity, rigor in personality were significantly correlated with the total score of SCL-90 ( r=0.542, -0.442, -0.204, -0.202 respectively, P<0.05). Coping style was significantly negatively correlated with the total score of SCL-90 ( r=-0.352, P<0.05). The father's refusal and denial, the mother's emotional warmth and understanding, the mother's excessive interference and protection in the parental rearing styles could predict the mental health of community correction offenders ( β=0.191, -0.163, 0.233 respectively, P<0.05). Coping styles had a negative predictive effect on mental health ( β=-0.352, P<0.05). Neuroticism, preciseness and extra-version in personality could predict mental health ( β=0.461, 0.183, -0.281 respectively, P<0.05). The pathway coefficient of parental rearing styles → mental health was 0.261 ( P<0.001), and the direct effect was significant. The confidence intervals of parental rearing styles → personality traits → mental health path, parental rearing styles → coping tendency → mental health path did not include 0, indicating that partial mediating effect of personality traits and coping tendency were significant. Conclusion:Personality traits and coping styles play a mediating role in the influence of parental rearing style on mental health of community correction offenders.