To target the pivotal BCR/ABL oncoprotein in chronic myeloid leukemia (CML) cells, tyrosine kinase inhibitors (TKIs) are utilized as landmark achievements in CML therapy. However, TKI resistance and intolerance remain principal obstacles in the treatment of CML patients. In recent years, drug repositioning provided alternative and promising perspectives apart from the classical cancer therapies, and promoted anthelmintic mebendazole (MBZ) as an effective anti-cancer drug in various cancers. Here, we investigated the role of MBZ in CML treatment including imatinib-resistant CML cells. Our results proved that MBZ inhibited the proliferation and induced apoptosis in CML cells. We found that MBZ effectively suppressed BCR/ABL kinase activity and MEK/ERK signaling pathway by reducing p-BCR/ABL and p-ERK levels with ABL1 targeting ability. Meanwhile, MBZ directly targeted the colchicine-binding site of β-tubulin protein, hampered microtubule polymerization and induced mitosis arrest and mitotic catastrophe. In addition, MBZ increased DNA damage levels and hampered the accumulation of ataxia-telangiectasia mutated and DNA-dependent protein kinase into the nucleus. This work discovered that anthelmintic MBZ exerts remarkable anticancer effects in both imatinib-sensitive and imatinib-resistant CML cells in vitro and revealed mechanisms underlying. From the perspective of drug repositioning and multi‐target therapeutic strategy, this study provides a promising option for CML treatment, especially in TKI-resistant or intolerant individuals.

To target the pivotal BCR/ABL oncoprotein in chronic myeloid leukemia (CML) cells, tyrosine kinase inhibitors (TKIs) are utilized as landmark achievements in CML therapy. However, TKI resistance and intolerance remain principal obstacles in the treatment of CML patients. In recent years, drug repositioning provided alternative and promising perspectives apart from the classical cancer therapies, and promoted anthelmintic mebendazole (MBZ) as an effective anti-cancer drug in various cancers. Here, we investigated the role of MBZ in CML treatment including imatinib-resistant CML cells. Our results proved that MBZ inhibited the proliferation and induced apoptosis in CML cells. We found that MBZ effectively suppressed BCR/ABL kinase activity and MEK/ERK signaling pathway by reducing p-BCR/ABL and p-ERK levels with ABL1 targeting ability. Meanwhile, MBZ directly targeted the colchicine-binding site of β-tubulin protein, hampered microtubule polymerization and induced mitosis arrest and mitotic catastrophe. In addition, MBZ increased DNA damage levels and hampered the accumulation of ataxia-telangiectasia mutated and DNA-dependent protein kinase into the nucleus. This work discovered that anthelmintic MBZ exerts remarkable anticancer effects in both imatinib-sensitive and imatinib-resistant CML cells in vitro and revealed mechanisms underlying. From the perspective of drug repositioning and multi‐target therapeutic strategy, this study provides a promising option for CML treatment, especially in TKI-resistant or intolerant individuals.

To target the pivotal BCR/ABL oncoprotein in chronic myeloid leukemia (CML) cells, tyrosine kinase inhibitors (TKIs) are utilized as landmark achievements in CML therapy. However, TKI resistance and intolerance remain principal obstacles in the treatment of CML patients. In recent years, drug repositioning provided alternative and promising perspectives apart from the classical cancer therapies, and promoted anthelmintic mebendazole (MBZ) as an effective anti-cancer drug in various cancers. Here, we investigated the role of MBZ in CML treatment including imatinib-resistant CML cells. Our results proved that MBZ inhibited the proliferation and induced apoptosis in CML cells. We found that MBZ effectively suppressed BCR/ABL kinase activity and MEK/ERK signaling pathway by reducing p-BCR/ABL and p-ERK levels with ABL1 targeting ability. Meanwhile, MBZ directly targeted the colchicine-binding site of β-tubulin protein, hampered microtubule polymerization and induced mitosis arrest and mitotic catastrophe. In addition, MBZ increased DNA damage levels and hampered the accumulation of ataxia-telangiectasia mutated and DNA-dependent protein kinase into the nucleus. This work discovered that anthelmintic MBZ exerts remarkable anticancer effects in both imatinib-sensitive and imatinib-resistant CML cells in vitro and revealed mechanisms underlying. From the perspective of drug repositioning and multi‐target therapeutic strategy, this study provides a promising option for CML treatment, especially in TKI-resistant or intolerant individuals.

To target the pivotal BCR/ABL oncoprotein in chronic myeloid leukemia (CML) cells, tyrosine kinase inhibitors (TKIs) are utilized as landmark achievements in CML therapy. However, TKI resistance and intolerance remain principal obstacles in the treatment of CML patients. In recent years, drug repositioning provided alternative and promising perspectives apart from the classical cancer therapies, and promoted anthelmintic mebendazole (MBZ) as an effective anti-cancer drug in various cancers. Here, we investigated the role of MBZ in CML treatment including imatinib-resistant CML cells. Our results proved that MBZ inhibited the proliferation and induced apoptosis in CML cells. We found that MBZ effectively suppressed BCR/ABL kinase activity and MEK/ERK signaling pathway by reducing p-BCR/ABL and p-ERK levels with ABL1 targeting ability. Meanwhile, MBZ directly targeted the colchicine-binding site of β-tubulin protein, hampered microtubule polymerization and induced mitosis arrest and mitotic catastrophe. In addition, MBZ increased DNA damage levels and hampered the accumulation of ataxia-telangiectasia mutated and DNA-dependent protein kinase into the nucleus. This work discovered that anthelmintic MBZ exerts remarkable anticancer effects in both imatinib-sensitive and imatinib-resistant CML cells in vitro and revealed mechanisms underlying. From the perspective of drug repositioning and multi‐target therapeutic strategy, this study provides a promising option for CML treatment, especially in TKI-resistant or intolerant individuals.

To target the pivotal BCR/ABL oncoprotein in chronic myeloid leukemia (CML) cells, tyrosine kinase inhibitors (TKIs) are utilized as landmark achievements in CML therapy. However, TKI resistance and intolerance remain principal obstacles in the treatment of CML patients. In recent years, drug repositioning provided alternative and promising perspectives apart from the classical cancer therapies, and promoted anthelmintic mebendazole (MBZ) as an effective anti-cancer drug in various cancers. Here, we investigated the role of MBZ in CML treatment including imatinib-resistant CML cells. Our results proved that MBZ inhibited the proliferation and induced apoptosis in CML cells. We found that MBZ effectively suppressed BCR/ABL kinase activity and MEK/ERK signaling pathway by reducing p-BCR/ABL and p-ERK levels with ABL1 targeting ability. Meanwhile, MBZ directly targeted the colchicine-binding site of β-tubulin protein, hampered microtubule polymerization and induced mitosis arrest and mitotic catastrophe. In addition, MBZ increased DNA damage levels and hampered the accumulation of ataxia-telangiectasia mutated and DNA-dependent protein kinase into the nucleus. This work discovered that anthelmintic MBZ exerts remarkable anticancer effects in both imatinib-sensitive and imatinib-resistant CML cells in vitro and revealed mechanisms underlying. From the perspective of drug repositioning and multi‐target therapeutic strategy, this study provides a promising option for CML treatment, especially in TKI-resistant or intolerant individuals.

Objective:To develop a training program for emergency airway management capabilities of anesthesia nurses based on a virtual simulation platform and to validate its effectiveness, providing a reference for airway training of anesthesia nurses.Methods:A quasi-experimental study was conducted. A total of 60 anesthesia nurses in Nanjing Drum Tower Hospital from August 2022 to August 2023 were selected as the research objects by convenience sampling method. According to the random number table method, they were divided into the control group and the experimental group, with 30 nurses in each group. The two groups had the same theoretical training. As for the operational training, the control group was taught by case combined with ordinary simulator, and the experimental group was taught by case combined with virtual simulation platform. The theoretical scores, skill operation scores, and satisfaction degree with airway management emergency response training were compared between the two groups of anesthesia nurses.Results:There were 4 males and 26 females in the control group, aged (24.37 ± 1.45) years. There were 6 males and 24 females in the experimental group, aged (24.20 ± 1.22) years.The theoretical scores and skill operation scores of the experimental group were 89.20 ± 3.99 and 90.10 ± 4.45, respectively, both higher than those of the control group, which were 84.83 ± 4.64 and 85.30 ± 5.64, showing statistically significant differences ( t=-3.91, -3.66, both P<0.05). In the self-evaluation of satisfaction degree with airway management emergency response training, the experimental group scored (16.67±1.79) for theoretical knowledge mastery, 18.37 ± 1.73 for skill operation mastery, 19.07 ± 1.17 for enthusiasm in airway training, 18.43 ± 1.48 for initiative in self-learning, and 18.00 ± 1.51 for the engagement of course design, all higher than 13.67 ± 2.17, 14.37 ± 2.34, 13.37 ± 2.63, 12.30 ± 3.51, and 12.77 ±2.71 in the control group, respectively. The differences were statistically significant ( t values were -10.83 to -5.84, all P<0.05). Conclusions:The airway management emergency training curriculum for nurse anesthetists developed based on a virtual simulation platform improved their theoretical knowledge and practical skills in airway emergency management. Additionally, satisfaction across various aspects of the training was markedly enhanced, providing a valuable reference for airway management training of nurse anesthetists and demonstrating considerable potential for clinical implementation.

Objective:To construct an evaluation index system for nursing quality of post-anesthesia visit based on service quality gap (SERVQUAL) model, providing a scientific basis for objectively evaluating the quality of post-anesthesia visit nursing.Methods:From October 2023 to February 2024, the SERVQUAL model was used as the theoretical basis for the preliminary formulation of an evaluation index system for nursing quality of post-anesthesia visit through literature search and semi-structured interviews. Delphi method was used to determine the indicators for evaluating the nursing quality of post-anesthesia visit after two rounds of consultation with 20 experts. Analytic hierarchy process was used to calculate the weights of indicators at all levels.Results:For two rounds of consultation, the valid recovery rate of the questionnaires was 100.00% (20/20). The judgment coefficient for expert was 0.93, the familiarity coefficient was 0.87, and the authority coefficient was 0.90. Kendall's harmony coefficients for two rounds of consultation were 0.173 and 0.192, respectively ( P<0.001). An evaluation index system for nursing quality of post-anesthesia visit was ultimately formed, including six first-level indicators, 14 second-level indicators, and 59 third-level indicators. The weights of the first-level indicators ranged from 0.097 to 0.279, the combined weights of the second-level indicators ranged from 0.019 to 0.138, and the combined weights of the third-level indicators ranged from 0.002 to 0.065. Conclusions:The evaluation index system for nursing quality of post-anesthesia visit based on the SERVQUAL model is scientific and practical, and can provide a certain basis for carrying out nursing quality evaluation for post-anesthesia visits in China.

Objective:To develop a training program for emergency airway management capabilities of anesthesia nurses based on a virtual simulation platform and to validate its effectiveness, providing a reference for airway training of anesthesia nurses.Methods:A quasi-experimental study was conducted. A total of 60 anesthesia nurses in Nanjing Drum Tower Hospital from August 2022 to August 2023 were selected as the research objects by convenience sampling method. According to the random number table method, they were divided into the control group and the experimental group, with 30 nurses in each group. The two groups had the same theoretical training. As for the operational training, the control group was taught by case combined with ordinary simulator, and the experimental group was taught by case combined with virtual simulation platform. The theoretical scores, skill operation scores, and satisfaction degree with airway management emergency response training were compared between the two groups of anesthesia nurses.Results:There were 4 males and 26 females in the control group, aged (24.37 ± 1.45) years. There were 6 males and 24 females in the experimental group, aged (24.20 ± 1.22) years.The theoretical scores and skill operation scores of the experimental group were 89.20 ± 3.99 and 90.10 ± 4.45, respectively, both higher than those of the control group, which were 84.83 ± 4.64 and 85.30 ± 5.64, showing statistically significant differences ( t=-3.91, -3.66, both P<0.05). In the self-evaluation of satisfaction degree with airway management emergency response training, the experimental group scored (16.67±1.79) for theoretical knowledge mastery, 18.37 ± 1.73 for skill operation mastery, 19.07 ± 1.17 for enthusiasm in airway training, 18.43 ± 1.48 for initiative in self-learning, and 18.00 ± 1.51 for the engagement of course design, all higher than 13.67 ± 2.17, 14.37 ± 2.34, 13.37 ± 2.63, 12.30 ± 3.51, and 12.77 ±2.71 in the control group, respectively. The differences were statistically significant ( t values were -10.83 to -5.84, all P<0.05). Conclusions:The airway management emergency training curriculum for nurse anesthetists developed based on a virtual simulation platform improved their theoretical knowledge and practical skills in airway emergency management. Additionally, satisfaction across various aspects of the training was markedly enhanced, providing a valuable reference for airway management training of nurse anesthetists and demonstrating considerable potential for clinical implementation.

Objective:To construct an evaluation index system for nursing quality of post-anesthesia visit based on service quality gap (SERVQUAL) model, providing a scientific basis for objectively evaluating the quality of post-anesthesia visit nursing.Methods:From October 2023 to February 2024, the SERVQUAL model was used as the theoretical basis for the preliminary formulation of an evaluation index system for nursing quality of post-anesthesia visit through literature search and semi-structured interviews. Delphi method was used to determine the indicators for evaluating the nursing quality of post-anesthesia visit after two rounds of consultation with 20 experts. Analytic hierarchy process was used to calculate the weights of indicators at all levels.Results:For two rounds of consultation, the valid recovery rate of the questionnaires was 100.00% (20/20). The judgment coefficient for expert was 0.93, the familiarity coefficient was 0.87, and the authority coefficient was 0.90. Kendall's harmony coefficients for two rounds of consultation were 0.173 and 0.192, respectively ( P<0.001). An evaluation index system for nursing quality of post-anesthesia visit was ultimately formed, including six first-level indicators, 14 second-level indicators, and 59 third-level indicators. The weights of the first-level indicators ranged from 0.097 to 0.279, the combined weights of the second-level indicators ranged from 0.019 to 0.138, and the combined weights of the third-level indicators ranged from 0.002 to 0.065. Conclusions:The evaluation index system for nursing quality of post-anesthesia visit based on the SERVQUAL model is scientific and practical, and can provide a certain basis for carrying out nursing quality evaluation for post-anesthesia visits in China.

Objective:To explore the nursing work items and the technical difficulty in post anesthesia care unit, so as to provide the basis for accurate calculation of anesthesia nursing workload and reasonable matching of human resources.Methods:The primary and secondary indicators of the nursing working system in post anesthesia care unit were determined through the literature review and group discussion. Delphi method was used to revise the indicators and evaluate the importance and difficulty degree of the indicators. Finally, analytic hierarchy process and linear weighted sum method were used to calculate the weight value and difficulty coefficient of each index.Results:The positive coefficients of the experts in the two rounds were 100.00%, the authority coefficients of the experts were 0.90 and 0.96, the variation coefficients ranged from 0.000 to 0.342 and 0.042 to 0.307, and the Kendall coefficients were 0.239 and 0.273 (both P<0.01). The evaluation index system of the difficulty of nursing work in post anesthesia care unit was finally composed of 10 primary indicators and 85 secondary indicators. The weight of the primary index ranged from 0.016 4 to 0.186 4, and the weight of the secondary index ranged from 0.000 8 to 0.064 3. The standardized difficulty coefficient of the secondary index ranged from 1.02 to 1.59. Conclusions:The evaluation index system of the difficulty of nursing work items in post anesthesia care unit was comprehensive and the difficulty coefficient was in line with the actual clinical work in this study, which provides reference for the follow-up scientific calculation of nursing workload and human resources matching in post anesthesia care unit.