BACKGROUND:Obesity and its relevant chronic inflammation are important risk factors for inducing type 2 diabetes.This inflammatory response will further involve skeletal muscle,leading to an increase in catabolic and autophagic fluxes in skeletal muscle.Aerobic exercise is the mainstream mode of exercise in the prevention and treatment of type 2 diabetes,and may also has a certain protective effect on skeletal muscle. OBJECTIVE:To explore the effects and regulatory mechanisms of aerobic exercise on glucolipid metabolism,skeletal muscle inflammation and autophagy in type 2 diabetic rats. METHODS:Animal models of type 2 diabetes were established in rats by 8-week high-fat feeding combined with streptozotocin injection,and the experimental rats were then divided into normal control group,normal exercise group,diabetic control group and diabetic exercise group.The exercise group performed 4 weeks of aerobic exercise(16 m/min,60 min/d,5 d/wk).The levels of blood glucose,high-density lipoprotein,low-density lipoprotein and triglyceride in serum were measured by an automated biochemical analyzer.Serum insulin level was determined using enzyme-linked immunosorbent assay and the insulin resistance index and area under the glucose metabolism curve were calculated.The levels of interleukin 6 and tumor necrosis factor α in skeletal muscle were measured by enzyme-linked immunosorbent assay after 4 weeks of aerobic exercise,and the expression levels of forkhead box protein O3(FoxO3),LC3 and p62 in skeletal muscle were measured by western blot assay. RESULTS AND CONCLUSION:The area under the glucose tolerance curve and insulin resistance index both increased significantly in type 2 diabetic rats(P<0.001,P=0.025),and aerobic exercise significantly reduced the area under the glucose tolerance curve and insulin resistance index in the normal exercise group(P<0.001,P=0.038)and diabetic exercise group(P<0.001,P=0.004).Serum high-density lipoprotein significantly decreased(P=0.030),and low-density lipoprotein and triglyceride(P=0.027,P=0.014)levels significantly increased in the diabetic control group compared with the normal control group.Aerobic exercise significantly reduced triglyceride and low-density lipoprotein levels in the normal exercise group(P=0.019,P=0.008)as well as triglyceride levels in the diabetic exercise group(P=0.022).Both interleukin-6 and tumor necrosis factor α levels were significantly increased in the skeletal muscle of type 2 diabetic rats compared with the normal control group(P<0.001,P=0.007),and aerobic exercise significantly reduced tumor necrosis factor α levels in the diabetic exercise group(P=0.017).The LC3-Ⅱ/LC3-I was significantly increased in the skeletal muscle of type 2 diabetic rats compared with the normal control group.Aerobic exercise significantly increased the LC3-Ⅱ/LC3-I in the normal exercise group(P<0.001)and decreased the LC3-Ⅱ/LC3-I,FoxO3 and p62 protein expression levels in the diabetic exercise group(P=0.026,P=0.050,P=0.048).To conclusion,type 2 diabetes model established by high-fat feeding combined with streptozotocin injection has obvious glycolipid metabolism disorder,and leads to inflammatory response and excessive activation of autophagy in skeletal muscle.Aerobic exercise can improve glycolipid metabolism,reduce local inflammation in skeletal muscle and inhibit autophagy,and finally play a protective role in skeletal muscle.

BACKGROUND:Epidemiological research data on sarcopenia in China are concentrated in some provincial capitals and developed areas,while research on sarcopenia in the elderly in the Yunnan-Guizhou Plateau is at the initial stage,and there is still a lack of understanding of the development mechanism,influencing factors and prevention methods of sarcopenia. OBJECTIVE:Based on the 2019 Asian Sarcopenia Working Group Standard(AWGS2019),to assess sarcopenia in the elderly in the Yunnan-Guizhou Plateau,while analyzing the prevalence and risk factors of sarcopenia in the elderly. METHODS:A total of 1 327 elderly study subjects(650 males and 677 females)were recruited and their socio-demographic characteristics,clinical data and physical activity levels were collected.The prevalence of sarcopenia was screened using the AWGS2019 criteria.The possible influencing factors for sarcopenia were screened by univariate analysis,including χ2 test for comparing count data and t-test for comparing measurement data,and the indicators through univariate screening were included in the binary logistic regression model to assess the risk factors for sarcopenia,and then the odds ratio(OR)and 95%confidence interval(CI)were calculated. RESULTS AND CONCLUSION:The prevalence of sarcopenia in the elderly in the Yunnan-Guizhou Plateau was 14.62%(12.73%male and 16.49%female).Aging(OR=1.158,95%CI:1.133-1.185),female(OR=2.416,95%CI:1.629-3.586),fasting blood glucose≥7.0 mmol/L(OR=1.653,95%CI:1.071-2.551),smoking(OR=1.595,95%CI:1.043-2.438)and a low physical activity level(OR=1.778,95%CI:1.154-2.737)were all independent risk factors for sarcopenia,while body mass index was a protective factor for sarcopenia(OR=0.708,95%CI:0.583-0.859).These findings indicate that aging,female,fasting glucose≥7.0 mmol/L,smoking and low physical activity levels all increase the risk of sarcopenia,while increased body mass index can decrease the risk of sarcopenia.To conclude,the elderly(especially women)should maintain a healthy lifestyle and slightly higher body mass index levels during the aging process,thus reducing the risk of sarcopenia.

On the basis of the qualitative preparation quality markers of Zhibao Sanbian Wan (ZBSBW), we screened out the quantitative markers and evaluated the content consistency of ZBSBW. A method capable of simultaneously determining 34 compounds in ZBSBW was established based on HPLC-MS/MS, and 16 batches of ZBSBW were simultaneously analyzed by this method. Furthermore, we explored a general strategy for analyzing the component migration in preparation, plasma, brain tissue and cerebrospinal fluid. The methodological investigation was confirmed by linear range, recovery (85.10%-105.07%), precision (RSD: 1.37%-4.58%), stability, and repeatability (3.00%-12.45%), the established method was suitable for the detection and quantification of the compounds in ZBSBW. The contents of compounds in ZBSBW were all lower than 1 mg·g^-1, and the contents and daily dose of nystose were the highest, followed by echinacoside, paeoniflorin, osthole and paeonol. The results of systematic clustering showed that the contents were consistent for ordinary preparations of ZBSBW. The principal component analysis showed that the components of berberine, ginsenoside Re, ginsenoside Rg1, pinoresinol diglucoside and tenuifolin had large variation, which contributed significantly to the grouping. The contents of echinacoside, verbascoside, polygalaxanthone Ⅲ, β-ecdysterone, osthole, alisol B 23-acetate, liquiritin and glycyrrhizic acid were stable from batch to batch. The animal experiment results showed that osthole, paeonol and liquiritin in ZBSBW could be absorbed into the blood and enter the brain tissue by passing through the blood-brain barrier. All animal studies were reviewed and approved by the Institutional Animal Care and Use Committee at Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences (No. 2020B071). The above compounds contributed the quantitative preparation quality markers of ZBSBW. In conclusion, the HPLC-MS/MS method established in this study was sensitive, accurate and rapid, and could be used for simultaneous quantification of 34 compounds and content consistency evaluation of multiple batches of preparations in ZBSBW. The result provided a methodological basis for the screening of quantitative preparation quality markers and material basis research of ZBSBW.

Gallbladder abdominal wall fistula is usually due to the acute cholecystitis with-out timely treatment, which leads the formation of abscess around the gallbladder, the gallbladder adhering to the abdominal wall and the abscess infiltrating into the skin to form a spontaneous abdominal wall fistula. Patient with gallbladder abdominal wall fistula may have the symptoms of cholecystolithiasis and acute cholecystitis. Ultrasound examination can detect the situation of gallbladder conveniently, including the internal echo after formation of abscess, the connection between the gallbladder and abdominal cavity, and the blood flow signal, to clarify the diagnosis for the subsequent treatment. The authors share the diagnosis and treatment experiences of an elderly patient with gallbladder abdominal wall fistula.

BACKGROUND: Maximal oxygen uptake (VO2 max) is the most direct, effective and objective index for determining cardiorespiratory endurance and exercise capacity, but there are few studies on the varying data of VO2max in rats for a long time. In addition, the effect of high-intensity interval training on the cardiorespiratory endurance in rats during age increasing has not yet been reported. OBJECTIVE: To investigate the improvement of 16-week high-intensity interval training on the cardiorespiratory endurance in rats, and to continuously measure the VO2 max for 16 weeks in 29-week-old rats, so as to provide data reference for the precise control of training intensity. METHODS: Twenty-eight male Wistar rats aged 29 weeks old were randomly assigned into a quiet control group (normal feeding, no training) and a high-intensity interval training group. The latter group underwent the interval exercise of high-intensity (90% VO2 max) and low-intensity (50% VO2 max), 5 times per week, for 16 weeks. The VO2 max and corresponding maximal running velocity were tested and compared every 2 weeks. The study was approved by the Ethics Committee of Sports Science and Physical Health Education Department, Beijing Sport University, with the approval No. 2015025. RESULTS AND CONCLUSION: (1) With the increasing age from 29 to 45 weeks, the VO2max fluctuated down-up-down. After 16 weeks of exercise, the VO2max was decreased by 31.6% in the high-intensity interval training group and 47.9% in the quiet control group. The VO2 max in the high-intensity interval training group was significantly higher than that in the quiet control group (P < 0.01). (2) The decrease of VO2 max in the high-intensity interval training group was significantly lower than that in the quiet control group at 6,8, and 16 weeks (P < 0.05 or P < 0.01). (3) The increase of maximal running velocity in the high-intensity interval training group was significantly higher than that in the quiet control group at 4 and 8 weeks (P < 0.05 or P < 0.01). (4) The maximal running velocity was positively correlated with VO2 max in rats. (5) These results suggest that age increasing is an irreversible factor, and high-intensity interval training can alleviate the decrease of cardiorespiratory endurance. Six-week high-intensity interval training can significantly improve the decrease of cardiorespiratory endurance in rats due to age increasing. Eight-week high-intensity interval training can significantly delay the decrease of cardiorespiratory endurance in rats due to age increasing. Four-week high-intensity interval training can promote the value of maximal running velocity and improve sports ability.

Objective:To investigate the abnormal physiological phenotype of hair follicles in mice with the Klhl24 gene initiation codon mutation, and to provide a basis for elucidating the regulatory mechanism of hair follicle development by the gene. Methods:A Klhl24 c.3G/T male mouse carrying a heterozygous mutation Klhl24c.3G>T in the initiation codon of the Klhl24 gene was produced by using clustered regularly interspaced short palindromic repeats (CRISPR) /Cas9 technology, and mated with 2 wild-type female mice. Then, the littermate mice were genotyped. The Klhl24 c.3G/T male mice and wild-type male mice served as the experimental group and control group respectively, and there were more than 3 mice in each group. On days 21 (the first telogen phase of hair cycle) and 45 (the second telogen phase of hair cycle) after birth, the tape sticking experiment was carried out. Immunohistochemical study of Ki67 expression was performed in skin tissues from the back of the mice, scanning electron microscopy and transmission electron microscopy were conducted to observe the hair root at the bottom of the hair shaft and the structure of mitochondria in skin tissues respectively, and a terminal deoxyribonucleotide transferase-mediated dUTP nick end labeling (TUNEL) kit was used to detect apoptosis in hair follicles. The Dunnett- t test was used for comparisons between the experimental group and control group. Results:The number of shed hair shafts per 0.25 cm 2 of the tape at the first and second telogen phases was significantly higher in the Klhl24 c.3G/T mice (1 224 ± 51.08, 1 514 ± 72.15 respectively) than in the wild-type mice (320 ± 55.68, 125 ± 2.86, t = 11.96, 19.24, respectively, both P < 0.001) . Scanning electron microscopy showed clubbed hair roots at the bottom of the shed hair shaft at the first telogen phase. On day 59 after birth, there was no new hair shaft growing on the back of the wild-type mice and the hair follicles were still at the telogen phase, while new hair shafts had grown on the back of the Klhl24 c.3G/T mice and the hair follicles had entered the next anagen phase. At the first telogen phase (i.e., on day 21) , transmission electron microscopy showed disordered structures of mitochondrial cristae in cells of the hair follicles in the skin tissues from the back of the Klhl24 c.3G/T mice, and the number of apoptotic cells in hair follicles (12 ± 1.15) was significantly higher in the Klhl24 c.3G/T mice than in the wild-type mice (3 ± 0.63, n = 8, t = 6.874, P < 0.001) . Conclusion:The abnormal hair phenotype of the Klhl24 c.3G/T mice mainly manifested as decreased anchoring ability of hair shafts at the telogen phase, precocious entry into the anagen phase, abnormal structure of mitochondria in hair follicle cells, and increased number of apoptotic cells.

To explore the feasibility of rapid proto-typing ( RP) technology in the treatment of Standford type A aortic dissection. 5 patients with Standford type A aortic dissection received computed tomography angiography (CTA) of their aortas. The CTA images were then processed by 3D reconstruction with MIMICS16. 0 in order to create aortic dissection models through RP technology based on real patient aorta size with a ratio of 1 ∶ 1 . Accord-ing to these models, the surgeons were able to fully understand the aortic diseases of patients, thus establish indi-vidualized treatment strategies for each patient. Besides, the surgeons simulated operations on the models, which helped them achieve better results in real surgery. These patients experienced no post-operative complications and were discharged from hospital with recovery. In conclusion, RP technology can provide adequate preoperotive prep-arations for patients with Standford type A aortic dissection, improve operation efficiency and accuracy in aortic dis-section and guide precise proximal anchoring of stents during intracavitary therapy.