Objective:To investigate the abnormal physiological phenotype of hair follicles in mice with the Klhl24 gene initiation codon mutation, and to provide a basis for elucidating the regulatory mechanism of hair follicle development by the gene. Methods:A Klhl24 c.3G/T male mouse carrying a heterozygous mutation Klhl24c.3G>T in the initiation codon of the Klhl24 gene was produced by using clustered regularly interspaced short palindromic repeats (CRISPR) /Cas9 technology, and mated with 2 wild-type female mice. Then, the littermate mice were genotyped. The Klhl24 c.3G/T male mice and wild-type male mice served as the experimental group and control group respectively, and there were more than 3 mice in each group. On days 21 (the first telogen phase of hair cycle) and 45 (the second telogen phase of hair cycle) after birth, the tape sticking experiment was carried out. Immunohistochemical study of Ki67 expression was performed in skin tissues from the back of the mice, scanning electron microscopy and transmission electron microscopy were conducted to observe the hair root at the bottom of the hair shaft and the structure of mitochondria in skin tissues respectively, and a terminal deoxyribonucleotide transferase-mediated dUTP nick end labeling (TUNEL) kit was used to detect apoptosis in hair follicles. The Dunnett- t test was used for comparisons between the experimental group and control group. Results:The number of shed hair shafts per 0.25 cm 2 of the tape at the first and second telogen phases was significantly higher in the Klhl24 c.3G/T mice (1 224 ± 51.08, 1 514 ± 72.15 respectively) than in the wild-type mice (320 ± 55.68, 125 ± 2.86, t = 11.96, 19.24, respectively, both P < 0.001) . Scanning electron microscopy showed clubbed hair roots at the bottom of the shed hair shaft at the first telogen phase. On day 59 after birth, there was no new hair shaft growing on the back of the wild-type mice and the hair follicles were still at the telogen phase, while new hair shafts had grown on the back of the Klhl24 c.3G/T mice and the hair follicles had entered the next anagen phase. At the first telogen phase (i.e., on day 21) , transmission electron microscopy showed disordered structures of mitochondrial cristae in cells of the hair follicles in the skin tissues from the back of the Klhl24 c.3G/T mice, and the number of apoptotic cells in hair follicles (12 ± 1.15) was significantly higher in the Klhl24 c.3G/T mice than in the wild-type mice (3 ± 0.63, n = 8, t = 6.874, P < 0.001) . Conclusion:The abnormal hair phenotype of the Klhl24 c.3G/T mice mainly manifested as decreased anchoring ability of hair shafts at the telogen phase, precocious entry into the anagen phase, abnormal structure of mitochondria in hair follicle cells, and increased number of apoptotic cells.

Objective To investigate the short-term efficacy and safety through analyzing the results of 11 cases of video assisted minimally invasive cardiac surgery. Methods Eleven patients who had underwent video assisted minimally invasive cardiac surgery in the second hospital of Anhui medical university from November 2017 to August 2018 were retrospectively analyzed. One patient underwent simple atrial septal defect repair, 6 patients underwent atrial septal defect repair+tricuspid valve repair, 3 patients underwent mitral valve replacement, and 1 patient underwent mitral valve repair + tricuspid valve repair. Results All patients underwent video cardiac surgery without death, with no major bleeding (need secondary thoracotomy to stop bleeding) and no neurological complications. One patient of incision fat liquefaction healed after dressing change. One patient developed lower limb artery stenosis, which was treated conservatively and discharged after hospitalization. Conclusions Video assisted minimally invasive cardiac surgery can be safely applied to simple congenital heart disease and valve surgery as long as patients are strictly screened. Due to the advantages of small trauma, less bleeding, and quick recovery, it is worth promoting.

Objective To observe the recent clinical effect of application of individual open fenestrated stent graft in type A aortic dissection without developing the greater curve of the arch. Method From December 2014 to November 2016,15 patients of type A aortic dissection without developing the greater curve of the arch un-derwent endovascular total arch replacement using individual open fenestrated stent graft in the Anhui Province Hospital.Among them,8 cases were only operated with open fenestrated stent graft in aortic arch, 7 cases with open fenestrated stent graft in aortic arch added 1 or 2 small stent graft.Result There was 1 postoperative death caused by severe low cardiac output. The rest of the patients were successfully discharged from the hospital, without ner-vous system and related complications. Follow-up computerized tomographic angiography showed all implanted stents were wide expansion and in a good position. No endoleaks and thrombus obliterated of the corresponding false lumen was found. Conclusion Individual open fenestrated stent graft is suitable for type A aortic dissection without developed the greater curve of the arch.Its significantly simplify the total arch replacement operation steps, reduce anastomotic and shorten the lower body arrest time. Consequently, reduce the risk of operation difficulty, postoperative blood loss and other viscera damage probability significantly. The early and middle term clinical re-sults is satisfactory.

Objective To investigate the clinical biomarkers of acute lung injury(ALI) after the Stanford A aortic dissection.Methods Thirty patients underwent Stanford A aoatic dissection were selected as subjects,who hospitalized from January 2006 to March 2013.Of which,21 patients underwent total arch replacement with stented elephant trunk procedure and 9 patients underwent triple-branched stent graft placement.The general information of patients,preoperation echocardiogram data,and arterial partial pressure of oxygen (PaO2),partial pressure of carbon dioxide (PaCO2) and fraction of inspired oxygen(FiO2) were recorded before,after the operation and entering ICU.Alveolar-arterial oxygen difference (A-aDO2),oxygenation index (OI) were calculated.Results A-aDO2 and OI at preoperation,postoperative and entering ICU point were (112.47 ±41.06) mmHg,(136.13 ± 29.51) mmHg and (141.37 ± 25.94) mmHg; (535.23 ± 70.15) mmHg; (491.50 ± 73.12) mmHg and (387.33 ± 91.32) mmHg respectively,and the differences were significant (F=35.926,323.742;P =0.000).The levels of A-aDO2 and OI at entering ICU were significant different from that of pre-operation and post-operation (P ＜ 0.01,P ＜ 0.05).Conclusion Early postoperative oxygenation and switching functions of patients with Stanford A aortic dissection are subject to damage to some degree.The A-aDO2 and OI might be sensitive biomarkers of the diagnosis for early acute lung injury of aortic dissection patients.