The welfare and ethics of laboratory animals are the ethical principles and behavioral norms that need to be followed in conducting animal-based scientific research, breeding and managing laboratory animals, and supervising and regulating such activities. The level of protection of laboratory animal welfare and ethics is closely related to the development of science and technology, which has become a widely recognized international consensus. At present, Guangdong Province is accelerating the construction of a high-level science and technology innovation province and the Guangdong-Hong Kong-Macao Greater Bay Area International Science and Technology Innovation Center. Guangdong Province should rely on its advanced governance capacity in the field of laboratory animal science and technology ethics to promote the high-quality development of its laboratory animal science and technology sector. Based on the management laws, regulations, and institutional mechanisms of laboratory animals in China, this paper explores the optimization of the laboratory animal science and technology ethics governance system, which includes the institutional guarantees, responsibility systems, ethical review and supervision mechanisms, and education and outreach. Through methods such as literature research, questionnaire surveys, and interview investigations, an empirical study of the laboratory animal science and technology ethics governance system in Guangdong Province has been conducted. Analysis of literature and research results shows that Guangdong Province has basically established a laboratory animal management system, collaboration mechanism, supervision mechanism, and education and training system that meet the current requirements of the laboratory animal science and technology ethics governance system in China. However, there are still problems such as an incomplete laboratory animal science and technology ethics supervision mechanism, an underdeveloped operation mechanism of review institutions, insufficient attention paid by laboratory animal units to the ethical review of animal experiments, inconsistent ethical review standards, and a lack of professional ethical education and training for ethics review personnel. Therefore, optimization measures such as improving the laboratory animal science and technology ethics review system, strengthening supervision and inspection, further strengthening the accountability of responsible entities, formulating review norms, and enhancing hierarchical and classified education and training are proposed, to provide a theoretical basis for promoting the normalized and long-term governance of laboratory animal science and technology ethics in Guangdong Province.

Fu's subcutaneous needling (FSN) represents a non-pharmacological therapy that employs disposable needles for subcutaneous application, primarily targeting the relaxation of tightened muscles, invigoration of blood circulation, and alleviation of local tissue ischemia. Originating from traditional acupuncture and modern techniques, FSN effectively treats muscle-related conditions, including soft tissue injuries, neck-shoulder-back pain, visceral pain, and non-painful diseases. Its unique features encompass shallow needling, the swaying movement technique, and an emphasis on governing the spirit. FSN is characterized by rapid therapeutic responses, high repeatability, and minimal side effects, adhering to the keep it simple, stupid (KISS) principle in research by emphasizing simplicity and efficacy. By mechanically releasing chronic muscle tension, FSN improves arterial blood flow, halts pathological leakage of intracellular adenosine triphosphate (ATP), and consequently resolves pain. This therapy offers a promising and safe approach for pain management and muscular health, deserving vigorous promotion and further scientific investigation.

OBJECTIVE: To investigate the current status of long-term quality of life in patients with severe acute pancreatitis (SAP) who have been cured and discharged, and to analyze the influencing factors affecting long-term quality of life in SAP cured patients after discharge. METHODS: A retrospective collection was conducted. Patients who were received standardized treatment before being cured and discharged from the hospital admitted to the first department of critical care medcine of the Second Affiliated Hospital of Anhui Medical University from January 2017 to December 2023 were enrolled. According to the 36-item short form health survey scale (SF-36) score, patients were divided into high score group (high quality of life, the top 50% of patients with total SF-36 score) and low score group (low quality of life, the bottom 50% of patients with total SF-36 score). The gender, age, history of hypertension and diabetes, etiology of pancreatitis, acute physiology and chronic health evaluation II (APACHE II), sequential organ failure assessment (SOFA), CT severity index (CTSI), laboratory indicators such as C-reactive protein (CRP), procalcitonin (PCT), blood glucose, and triglycerides upon admission, use of vasoactive drugs, non-invasive/high-flow ventilation, invasive ventilation, retroperitoneal puncture and drainage, open pancreatic surgery treatment and secondary infection during hospitalization were collected, as well as the retention of abdominal drainage tubes at discharge from hospital. Distribute follow-up questionnaires or telephone follow-up surveys through WeChat and Question Star programs to investigate the pancreatic secretion function, chronic abdominal pain, and recurrence of pancreatitis of patients after discharge. Multivariable Logistic regression was used to analyze the relevant factors affecting the long-term quality of life of cured patients with SAP. RESULTS: A total of 86 patients were ultimately enrolled. There were 43 patients in both the high and low score groups. Among 86 patients, 20 experienced acute pancreatitis recurrence, with a recurrence rate of 23.26%. Twenty-two (25.58%) experienced chronic abdominal pain after discharge, and 5 patients (5.81%) needed medication to relieve pain. Thirty-three patients (38.37%) had pancreatic exocrine dysfunction after discharge, characterized by abdominal distension, constipation or diarrhea. Twenty-two patients (25.58%) suffered from pancreatic endocrine dysfunction, and were diagnosed with diabetes. Univariate analysis showed that compared with the high score group, the low score group had more patients with hypertension, initial renal dysfunction, initial severe metabolic acidosis, initial serum calcium < 2.0 mmol/L, blood glucose > 11.1 mmol/L and cultured Gram positive bacteria (from blood/body fluid/pancreatic necrotic tissue) during treatment (48.84% vs. 16.28%, 60.47% vs. 32.56%, 18.60% vs. 4.65%, 88.37% vs. 62.79%, 55.81% vs. 30.23%, 34.88% vs. 13.95%), had higher CTSI score (6.60±1.61 vs. 5.77±1.32), lower hemoglobin level at discharge (g/L: 102.30±18.78 vs. 110.72±16.68), and a lower proportion of etiological interventions after discharge (34.88% vs. 67.44%), the differences were statistically significant (all P < 0.05). Multivariate Logistic regression analysis showed that hypertension [odds ratio (OR) = 4.814, 95% confidence interval (95%CI) was 1.196-19.378], initial serum calcium < 2.0 mmol/L (OR = 6.688, 95%CI was 1.321-33.873) and initial blood glucose > 11.1 mmol/L (OR = 6.473, 95%CI was 1.399-29.950) were risk factors for long-term quality of life in cured SAP patients (all P < 0.05), while post discharge prophylactic intervention was a protective factor for long-term quality of life (OR = 0.092, 95%CI was 0.020-0.425, P < 0.01). CONCLUSIONS Cured SAP patients have varying degrees of impaired secretion function and the possibility of recurrence of acute pancreatitis. Hypertension, initial serum calcium < 2.0 mmol/L and blood glucose > 11.1 mmol/L are independent influencing factors for low long-term quality of life in cured SAP patients. Prevention and intervention targeting the etiology of pancreatitis after discharge can improve the long-term quality of life of cured SAP patients.
Humans ; Quality of Life ; Retrospective Studies ; Pancreatitis/therapy* ; Patient Discharge ; Male ; Female ; Middle Aged ; APACHE ; Adult ; Acute Disease ; Aged

Objective To evaluate the sensitivity,repeatability,anti-degradation ability,in vivo temporal stability,and tissue specificity of the DNA methylation sequencing panel constructed in our previous study for height inference,so as to provide a reference for forensic application.Methods Sensitivity:different initial template quantities(50 ng,40 ng,30 ng,20 ng)were set for sequencing.Repeatability:DNA from the same sample was sequenced three times.Anti-degradation ability:whole blood was used to make blood stains,and DNA was extracted and sequenced at 0,3,6 and 9 months,respectively.In vivo temporal stability:the blood was collected at 0,3,6,and 9 months for sequencing.Tissue specificities:published data and findings were used to analyze the tissue specificities of CpGs in the panel.Results The sensitivity test showed that the initial template quantities of 20 ng detected all the CpG sites and still obtained accurate prediction results.The results of the three repeated predictions of the same sample are stable,and the differences are mainly due to the randomness of the DNN model,indicating good detection repeatability.A complete methylation profile was obtained for the blood stains left at room temperature for 9 months,and the predicted results showed a small range of fluctuations.The three samples were predicted to fluctuate within a range of 1.5 cm or less over nine months.Tissue-specific analyses showed a high correlation between blood and saliva,but can not apply to other tissues.Conclusion The DNA methylation detection system we developed in our previous study has good sensitivity,repeatability,anti degradation ability,in vivo time stability,as well as strong tissue specificity,making it suitable for height inference of blood samples.This supports the feasibility of using targeted DNA methylation analysis on whole blood samples to infer height in the field of forensic science.

Infectious disease animal models serve as indispensable tools for understanding the transmission patterns,pathogenesis,and anti-infective medicine.During the preparation and application of infectious animal disease models,situations inevitably arise that violate animal welfare and ethics,such as animal pain,suffering,and distress.Considering the biosafety factors,animal mortality is still used as the experimental endpoint in most experiments on infectious animals,which poses extremely high requirements for animal welfare and ethics.It is imperative to establish guiding principles or norms for the welfare and ethics of infectious animal experiments.Based on the fundamental principles of the welfare and ethics of experimental animals,this paper explores the special welfare and ethical requirements in infectious animal experiments.It emphasizes that infectious animal experiments should fully consider the balance among the scientific objectives of the research plan,animal welfare and ethics,and occupational health and safety of personnel.Based on literature research and comparative analysis of the welfare and ethical requirements of conventional animal experiments,special welfare and ethics requirements for infectious animal experiments are proposed,including personnel requirements,experimental animal selection standards,living environment management and equipment,special care and veterinary care,and humane endpoints.Personnel are required to undergo effective biosafety training,and sufficient authority should be granted to the Institutional Animal Care and Use Committee(IACUC),veterinarians,and veterinary technicians to ensure the implementation of animal welfare and ethics practices.The selection of laboratory animals should fully consider the requirements of research objectives,welfare,ethics,and biosafety,with the susceptibility and body size of laboratory animals being the key concerns in high-level biosafety laboratories.It is also clarified that the humane endpoint is an indispensable element of welfare and ethics in infectious animal experiments.Environmental enrichment and special care are necessary guarantees for achieving animal welfare and ethics.Therefore,this study can serve as a reference for relevant work.

Infectious disease animal models serve as indispensable tools for understanding the transmission patterns,pathogenesis,and anti-infective medicine.During the preparation and application of infectious animal disease models,situations inevitably arise that violate animal welfare and ethics,such as animal pain,suffering,and distress.Considering the biosafety factors,animal mortality is still used as the experimental endpoint in most experiments on infectious animals,which poses extremely high requirements for animal welfare and ethics.It is imperative to establish guiding principles or norms for the welfare and ethics of infectious animal experiments.Based on the fundamental principles of the welfare and ethics of experimental animals,this paper explores the special welfare and ethical requirements in infectious animal experiments.It emphasizes that infectious animal experiments should fully consider the balance among the scientific objectives of the research plan,animal welfare and ethics,and occupational health and safety of personnel.Based on literature research and comparative analysis of the welfare and ethical requirements of conventional animal experiments,special welfare and ethics requirements for infectious animal experiments are proposed,including personnel requirements,experimental animal selection standards,living environment management and equipment,special care and veterinary care,and humane endpoints.Personnel are required to undergo effective biosafety training,and sufficient authority should be granted to the Institutional Animal Care and Use Committee(IACUC),veterinarians,and veterinary technicians to ensure the implementation of animal welfare and ethics practices.The selection of laboratory animals should fully consider the requirements of research objectives,welfare,ethics,and biosafety,with the susceptibility and body size of laboratory animals being the key concerns in high-level biosafety laboratories.It is also clarified that the humane endpoint is an indispensable element of welfare and ethics in infectious animal experiments.Environmental enrichment and special care are necessary guarantees for achieving animal welfare and ethics.Therefore,this study can serve as a reference for relevant work.

Objective To evaluate the sensitivity,repeatability,anti-degradation ability,in vivo temporal stability,and tissue specificity of the DNA methylation sequencing panel constructed in our previous study for height inference,so as to provide a reference for forensic application.Methods Sensitivity:different initial template quantities(50 ng,40 ng,30 ng,20 ng)were set for sequencing.Repeatability:DNA from the same sample was sequenced three times.Anti-degradation ability:whole blood was used to make blood stains,and DNA was extracted and sequenced at 0,3,6 and 9 months,respectively.In vivo temporal stability:the blood was collected at 0,3,6,and 9 months for sequencing.Tissue specificities:published data and findings were used to analyze the tissue specificities of CpGs in the panel.Results The sensitivity test showed that the initial template quantities of 20 ng detected all the CpG sites and still obtained accurate prediction results.The results of the three repeated predictions of the same sample are stable,and the differences are mainly due to the randomness of the DNN model,indicating good detection repeatability.A complete methylation profile was obtained for the blood stains left at room temperature for 9 months,and the predicted results showed a small range of fluctuations.The three samples were predicted to fluctuate within a range of 1.5 cm or less over nine months.Tissue-specific analyses showed a high correlation between blood and saliva,but can not apply to other tissues.Conclusion The DNA methylation detection system we developed in our previous study has good sensitivity,repeatability,anti degradation ability,in vivo time stability,as well as strong tissue specificity,making it suitable for height inference of blood samples.This supports the feasibility of using targeted DNA methylation analysis on whole blood samples to infer height in the field of forensic science.

OBJECTIVE To provide a reference for accurately identifying and treating adverse reactions of osteonecrosis induced by bevacizumab. METHODS Clinical pharmacists participated in the treatment process of an adult patient with glioma who developed femoral head necrosis after the use of bevacizumab. By searching the FDA Adverse Event Reporting System （FAERS） and consulting relevant literature and drug instructions， the association between femoral head necrosis and bevacizumab was assessed. Medication recommendations were provided to the physician， and medication educations were provided to the patient. RESULTS The association between the patient’s femoral head necrosis and bevacizumab was assessed as “possibly related”. The physicians accepted the clinical pharmacists’ advice to discontinue bevacizumab and switched to anlotinib to continue antitumor treatment. The patient also followed the clinical pharmacists’ advice to avoid prolonged standing. Upon re-examination， the patient’s pain symptoms were under control， and the femoral head necrosis had not progressed. CONCLUSIONS Patients who receive long-term bevacizumab should attach great importance to bone pain and osteonecrosis， undergo regular imaging examinations， and detect and treat symptoms early to prevent the occurrence of severe osteonecrosis.

In the field of biomedicine,two-dimensional(2D)cell lines and animal models have played an im-portant role in the study of cell pathways and drug targets.However,due to species differences between humans and other animals,and the lack of hierarchy,cellular diversity,and cell-cell or cell-matrix interactions,2D cell lines could not ful-ly reflect what cells actually look like in the human body.Organoids are three-dimensional(3D)in vitro culture models de-rived from autologous tissue stem cells,which make up for the defects of 2D culture and can simulate the structure and function of real human organs to a certain extent,providing new ideas for disease diagnosis and treatment.Among them,lung organoids(LO)are a typical case studying the development process of human lung and the generation principle of lung diseases.Relevant studies have provided help for the treatment of pulmonary fibrosis,lung cancer,lung injury and other diseases.This paper aims to summarize and analyze the research progress of lung organoids in recent years,and fur-ther summarize the application of LO in the diagnosis and treatment of lung diseases.

Panapoptosis is a new focus of current research, which is essentially the inflammatory programmed cell death, with the common characteristics of pyrodeath, apoptosis and necrotic apoptosis. Panapoptosis is closely related to many diseases, such as infectious diseases, tumors, neurodegenerative diseases and so on. As a pathway of recognition and activation by the immune system, panapoptosis plays an important role in controlling the spread of inflammation and disease progression, although the specific regulatory mechanism is not yet clear. This review aims to explore the role of panapoptosis in the pathogenesis and development of cellular inflammation and diseases, and seek potential intervention targets to provide new strategies for clinical disease treatment.