Objective To investigate the clinical outcome differences between robotic-assisted intramedullary nailing and traditional manual surgery,and to analyze the advantages and feasibility of robotic-assisted intramedullary nail fixation in the treatment of intertrochanteric fractures in elderly patients.Methods From December 2023 to December 2024,elderly patients with intertrochanteric fractures who underwent surgery at Department of Trauma Orthopedics,Beijing Luhe Hospital,Capital Medical University were included.Patients were divided into two groups based on the surgical method.The robotic-assisted group underwent robotic-assisted intramedullary nail fixation,while the traditional group received manual intramedullary nail fixation.Baseline data and observation indicators were collected and compared between the two groups to assess any differences.Results There were no statistically significant differences in baseline data between the two groups(P>0.05).The intraoperative blood loss in the robotic-assisted group was(94.28±9.43)mL,compared to(143.00±11.11)mL in the traditional group(P<0.001).The operative time in the robotic-assisted group was(53.06±9.89)min,while in the traditional group,it was(66.74±10.18)min(P<0.001).The skin incision length for the main nail in the robotic-assisted group was(3.23±0.64)cm,whereas in the traditional group,it was(4.03±0.79)cm(P<0.01).Postoperative hemoglobin levels in the robotic-assisted group decreased by(12.63±4.27)g/L,compared to(17.29±4.32)g/L in the traditional group(P=0.018).At 6 months postoperatively,the Harris hip scores in the robotic-assisted group showed 30 cases of excellent,10 good,and 3 poor outcomes,while in the traditional group,there were 22 excellent,15 good,and 6 poor cases(P=0.198).Conclusion Robotic-assisted intramedullary nailing for intertrochanteric fractures offers advantages such as minimally invasive and precise procedures,shorter operative times,and reduced blood loss.Compared to traditional surgical methods,it demonstrates certain benefits in reducing postoperative complications in elderly patients.

Objective To investigate the clinical outcome differences between robotic-assisted intramedullary nailing and traditional manual surgery,and to analyze the advantages and feasibility of robotic-assisted intramedullary nail fixation in the treatment of intertrochanteric fractures in elderly patients.Methods From December 2023 to December 2024,elderly patients with intertrochanteric fractures who underwent surgery at Department of Trauma Orthopedics,Beijing Luhe Hospital,Capital Medical University were included.Patients were divided into two groups based on the surgical method.The robotic-assisted group underwent robotic-assisted intramedullary nail fixation,while the traditional group received manual intramedullary nail fixation.Baseline data and observation indicators were collected and compared between the two groups to assess any differences.Results There were no statistically significant differences in baseline data between the two groups(P>0.05).The intraoperative blood loss in the robotic-assisted group was(94.28±9.43)mL,compared to(143.00±11.11)mL in the traditional group(P<0.001).The operative time in the robotic-assisted group was(53.06±9.89)min,while in the traditional group,it was(66.74±10.18)min(P<0.001).The skin incision length for the main nail in the robotic-assisted group was(3.23±0.64)cm,whereas in the traditional group,it was(4.03±0.79)cm(P<0.01).Postoperative hemoglobin levels in the robotic-assisted group decreased by(12.63±4.27)g/L,compared to(17.29±4.32)g/L in the traditional group(P=0.018).At 6 months postoperatively,the Harris hip scores in the robotic-assisted group showed 30 cases of excellent,10 good,and 3 poor outcomes,while in the traditional group,there were 22 excellent,15 good,and 6 poor cases(P=0.198).Conclusion Robotic-assisted intramedullary nailing for intertrochanteric fractures offers advantages such as minimally invasive and precise procedures,shorter operative times,and reduced blood loss.Compared to traditional surgical methods,it demonstrates certain benefits in reducing postoperative complications in elderly patients.

SARS-CoV-2 infection causes complicated clinical manifestations with variable multi-organ injuries, however, the underlying mechanism, in particular immune responses in different organs, remains elusive. In this study, comprehensive transcriptomic alterations of 14 tissues from rhesus macaque infected with SARS-CoV-2 were analyzed. Compared to normal controls, SARS-CoV-2 infection resulted in dysregulation of genes involving diverse functions in various examined tissues/organs, with drastic transcriptomic changes in cerebral cortex and right ventricle. Intriguingly, cerebral cortex exhibited a hyperinflammatory state evidenced by significant upregulation of inflammation response-related genes. Meanwhile, expressions of coagulation, angiogenesis and fibrosis factors were also up-regulated in cerebral cortex. Based on our findings, neuropilin 1 (NRP1), a receptor of SARS-CoV-2, was significantly elevated in cerebral cortex post infection, accompanied by active immune response releasing inflammatory factors and signal transmission among tissues, which enhanced infection of the central nervous system (CNS) in a positive feedback way, leading to viral encephalitis. Overall, our study depicts a multi-tissue/organ transcriptomic landscapes of rhesus macaque with early infection of SARS-CoV-2, and provides important insights into the mechanistic basis for COVID-19-associated clinical complications.
Animals ; COVID-19/genetics* ; Macaca mulatta ; SARS-CoV-2/genetics* ; Transcriptome

Objective:To explore the effects of immersion hypothermia and body temperature afterdrop during rewarming on animal bodies by establishing animal body temperature afterdrop model.Methods:The mini-swine were submerged in the simulated low temperature seawater (1.0℃) from the chest down. When their body temperatures dropped to 28.0℃, they were immediately removed from the seawater and placed in the warm water of 35.0℃ for rewarming. The body temperature, electrocardiogram, and heart rate were real-time monitored. Blood samples were collected before the mini-swine entering the seawater, when being removed from the seawater, and when the ventricular fibrillation occurred as a result of body temperature afterdrop. The levels of blood glucose, platelets, blood urea nitrogen, creatinine, serum potassium, total bilirubin, lactate dehydrogenase (LDH), aspartate aminotransferase (AST), cold shock protein (CSP), and adrenaline were measured.Results:As the seawater immersion went on, the body temperatures of the mini-swine gradually decreased and their heart rates gradually increased. But the heart rates began to decrease when the body temperature dropped to an average of 35.5℃. During rewarming, the body temperature continued to drop, then began to rise from the lowest average of 25.3℃. The average body temperature afterdrop was 2.7℃. The heart rates began to rise from the lowest average of 50 beats per minute. When the body temperature rose to an average of 37.1℃, the heart rates returned to an average of 112 beats per minute. Compared with the values before seawater immersion, the blood glucose, blood urea nitrogen, creatinine, total bilirubin, serum potassium, AST, and CSP of the mini-swine were significantly increased when they were removed from the seawater, with statistically significant differences ( P<0.05). Compared with the values at the removal from seawater, when the ventricular fibrillation caused by the afterdrop occurred, the blood glucose level was significantly reduced, while the values of blood urea nitrogen, creatinine, total bilirubin, and AST were significantly increased, with statistically significant differences ( P<0.05), but the values of platelet, LDH, and adrenaline had no significant changes. Conclusion:Low temperature seawater immersion and body temperature afterdrop significantly affect heart rate, blood glucose, and liver and kidney functions, which can provide a theoretical basis for preventing and treating temperature afterdrop during rewarming in patients with hypothermia.

Objective:To explore the effects of immersion hypothermia and body temperature afterdrop during rewarming on animal bodies by establishing animal body temperature afterdrop model.Methods:The mini-swine were submerged in the simulated low temperature seawater (1.0℃) from the chest down. When their body temperatures dropped to 28.0℃, they were immediately removed from the seawater and placed in the warm water of 35.0℃ for rewarming. The body temperature, electrocardiogram, and heart rate were real-time monitored. Blood samples were collected before the mini-swine entering the seawater, when being removed from the seawater, and when the ventricular fibrillation occurred as a result of body temperature afterdrop. The levels of blood glucose, platelets, blood urea nitrogen, creatinine, serum potassium, total bilirubin, lactate dehydrogenase (LDH), aspartate aminotransferase (AST), cold shock protein (CSP), and adrenaline were measured.Results:As the seawater immersion went on, the body temperatures of the mini-swine gradually decreased and their heart rates gradually increased. But the heart rates began to decrease when the body temperature dropped to an average of 35.5℃. During rewarming, the body temperature continued to drop, then began to rise from the lowest average of 25.3℃. The average body temperature afterdrop was 2.7℃. The heart rates began to rise from the lowest average of 50 beats per minute. When the body temperature rose to an average of 37.1℃, the heart rates returned to an average of 112 beats per minute. Compared with the values before seawater immersion, the blood glucose, blood urea nitrogen, creatinine, total bilirubin, serum potassium, AST, and CSP of the mini-swine were significantly increased when they were removed from the seawater, with statistically significant differences ( P<0.05). Compared with the values at the removal from seawater, when the ventricular fibrillation caused by the afterdrop occurred, the blood glucose level was significantly reduced, while the values of blood urea nitrogen, creatinine, total bilirubin, and AST were significantly increased, with statistically significant differences ( P<0.05), but the values of platelet, LDH, and adrenaline had no significant changes. Conclusion:Low temperature seawater immersion and body temperature afterdrop significantly affect heart rate, blood glucose, and liver and kidney functions, which can provide a theoretical basis for preventing and treating temperature afterdrop during rewarming in patients with hypothermia.

Mexico has close economic cooperation with China, which boosts with rich herbal medicine resources. Its traditional medicine share great similarity with Traditional Chinese Medicine (TCM) in both theory and form. Some of its traditional medicine and Chinese herbal medicine are the same in practice. The Mexico government pays great attention to the higher education of traditional medicine, and people are easy to accept traditional medicine treatment. The development of acupuncture and moxibustion in Mexico is good. Thus, to develop the service trade of TCM in Mexico has obvious advantages. At present, the local laws and regulations on TCM are not perfect. There are some problems in the development of TCM service trade, such as the lack of cross-border delivery platform, the small scale of business, and the weak attraction of natural person flow. Based on the concept of global health governance, this paper puts forward some measures to promote the development of TCM in Mexico, such as developing industrial chain or relying on overseas traditional medicine center.

ObjectiveTo investigate the effects of over expression of miR-34a on cellular proliferation and migration in bladder cancer cell line J82 by targeting Notchl.MethodsmiR-34a was predicted as a putative gene which can target Notchl through bioinformatics analysis,qRT-PCR and Western blot were performed to measure the expression levels of Notchl and miR-34a in invasive transitional cell carcinoma of bladder (TCCB) tissues and J82 cells transfected with miR-34a.Luciferase assay was employed to determine if miR-34a could target Notchl through binding to the 3'-untranslated region (3'UTR) of Notchl mRNA.J82 cells were transfected with pcDNA3.0-miR-34a or pcDNA3.0 control plasmid.MTS colorimetry was used to evaluate the effect of miR-34a on cell proliferation.The effect of miR-34a on cell migration was assessed by transwell migration assay.ResultsThe expression level of miR-34 in invasive TCCB tissues was lower than in adjacent bladder tissues (0.016(0.018) vs 0.042 (0.059),N =16; P =0.0006).On the contrary,the average levels of Notchl mRNA and protein were higher in tumors than in adjacent bladder tissues (2.765(2.156) vs 2.312(1.365),N =16; P =0.0025 and 0.857 ±0.197 vs 0.648 ±0.171 ;P ＜0.0001 ).After the transfection of miR-34a,the expressive level of miR-34a in J82 was highly induced ( (2.408 ±0.789) × 10-4 vs(0.153 ±0.029) × 10-4; P =0.0026).However,the expressive levels of Notchl mRNA and protein were obviously decreased (3.001 ± 0.106 vs 4.998 ± 1.053 ; P =0.0308 and 0.747 ± 0.050 vs 0.988 ± 0.102 ; P =0.0215 ).The results of luciferase assay showed that firefly activity was highly dimished (0.422 ± 0.028 vs 2.392 ± 0.148 ; P ＜ 0.0001 ).Cellular proliferation was inhibited after the transfection of miR-34a in J82 (P ＜ 0.0001 ).Moreover,number of migration cells of J82 was significantly reduced after the ectopic expression of miR-34a ( 179.3 ± 21.02 vs 269.7 ± 23.71 ; P =0.0078 ).ConclusionsmiR-34a inhibits the cellular proliferation and migration of bladder cancer cell line J82 via binding to the 3UTR of Notchl mRNA.

Objective:To explore th five year survivals and some prognostic factors for bres at cancer patients in the north areas of China,and the indentification or differ e nces on these variables among breast cancer patients between in China and in Can ada.Methods:All Data were collected from the hospital records of 1 002 breast cancer patients who were initially treated at the First Hospital of Jilin Uni versity (116 cases FTH,Changchun China) and the Sain t-Sacrement Hospital (886 cases in SSH,Quebec Canada) respectively by use of Historical Cohort survey,and the survival propotions were calculated and comp ared stradly by use of Kaplan-Meier method.Results:Age at diagnosis was substantially lower (average of age about 10 years less) among breast cancer patients seen at FTH compared to those treated at SSH (P<0.0001).Patients in the two hospitals differed in respect to tumor size at pathology (P=0.036).The proportion of women with lymph node involvement was greater at FTH (61.1%) than that at HSS(37.3%)(P<0.0001).Surgical treat ment of breats cancer was varied considerably:the radical mastectomy was frequen tly performed for andy stage of breast cancer patients in Changchun,but the part ial mastectomy was mainly used for patients with stage Ⅰ or Ⅱ in Quebec.The fi ve year survival was 74.2% among breast cacer patients seen at FTH compared to 7 6.3% among women treated at HSS,and there was no singnificant differrence (P =0.302). Conclusion:Five year survival of breast cancer patients treated surgically in C hangchun,China,was similar to that of patients treated surgically in Quebec,Can ada except for differences in age at diagnosis,tumor size and lymph node involve ment