Objective:To explore the effect of CO2 fractional laser combined with glucocorticoid(GC)on T follicular helper cell(Tfh)and B cell subsets in patients with advanced vitiligo(VL).Methods:Patients with advanced VL(128 cases)who were treated in Affiliated Hospital of Hebei University of Engineering from August 2019 to March 2022 were selected as research objects,and divided into group A(64 cases,CO2 fractional laser combined with GC)and group B(64 cases,CO2 fractional laser)according to different treatment methods,clinical efficacy and safety of the two groups were compared.Another healthy subjects(64 cases)were selected as control group.Collected clinical data of the three groups of subjects for comparative analysis.Randomized walking model was used to evaluate the effect of CO2 fractional laser combined with GC on immune function and inflammatory response in patients with advanced VL.Results:Compared with group B,group A had a higher clinical total effective rate(P<0.05)and a lower probability of adverse reactions(P<0.05).After treatment,compared with control group,proportion of Tfh2 and Tfh17 in group A had no significant difference(P>0.05),while proportion of Tfh2 and Tfh17 in group B still had significant difference(P<0.05);compared with group B,proportion of Tfh2 in group A after treatment was higher(P<0.05),while proportion of Tfh17 type was lower(P<0.05).After treat-ment,compared with control group,proportion of memory transformed B cell in group B was still significantly different(P<0.05).After treatment,compared with control group,levels of IgA,IgE and IgM in group B were still significantly different(P<0.05);compared with group B,level of IgM in group A was higher after treatment(P<0.05).After treatment,compared with control group,levels of IL-21 and IL-1β in group B were still significantly different(P<0.05);compared with group B,level of IL-10 in group A after treatment was higher(P<0.05),while levels of IL-21 and IL-1β were lower(P<0.05).Evaluation results of random walking model showed that the improvement of immune function and inflammatory reaction in group A was better than that in group B.Conclusion:CO2 fractional laser combined with GC can improve the immune function and inflammatory reaction of patients with advanced VL better after treatment,and the probability of adverse reactions is lower.

Objective:To explore the effect of CO2 fractional laser combined with glucocorticoid(GC)on T follicular helper cell(Tfh)and B cell subsets in patients with advanced vitiligo(VL).Methods:Patients with advanced VL(128 cases)who were treated in Affiliated Hospital of Hebei University of Engineering from August 2019 to March 2022 were selected as research objects,and divided into group A(64 cases,CO2 fractional laser combined with GC)and group B(64 cases,CO2 fractional laser)according to different treatment methods,clinical efficacy and safety of the two groups were compared.Another healthy subjects(64 cases)were selected as control group.Collected clinical data of the three groups of subjects for comparative analysis.Randomized walking model was used to evaluate the effect of CO2 fractional laser combined with GC on immune function and inflammatory response in patients with advanced VL.Results:Compared with group B,group A had a higher clinical total effective rate(P<0.05)and a lower probability of adverse reactions(P<0.05).After treatment,compared with control group,proportion of Tfh2 and Tfh17 in group A had no significant difference(P>0.05),while proportion of Tfh2 and Tfh17 in group B still had significant difference(P<0.05);compared with group B,proportion of Tfh2 in group A after treatment was higher(P<0.05),while proportion of Tfh17 type was lower(P<0.05).After treat-ment,compared with control group,proportion of memory transformed B cell in group B was still significantly different(P<0.05).After treatment,compared with control group,levels of IgA,IgE and IgM in group B were still significantly different(P<0.05);compared with group B,level of IgM in group A was higher after treatment(P<0.05).After treatment,compared with control group,levels of IL-21 and IL-1β in group B were still significantly different(P<0.05);compared with group B,level of IL-10 in group A after treatment was higher(P<0.05),while levels of IL-21 and IL-1β were lower(P<0.05).Evaluation results of random walking model showed that the improvement of immune function and inflammatory reaction in group A was better than that in group B.Conclusion:CO2 fractional laser combined with GC can improve the immune function and inflammatory reaction of patients with advanced VL better after treatment,and the probability of adverse reactions is lower.

Objective:To understand the influence of parenting style and life events on children and adolescents′ emotional behavior problems.Methods:The Achenbach′s Child Behavior Checklist (CBCL), Egma Minnen av Bardndosnauppforstran (EMBU), and Adolescent Self-Rating Life Events Check List (ASLEC) were used to investigate the children′s behavior problems, parenting style, and stressful life events among 420 children and adolescents (aged 6-16 years) in Beijing. Logistic regression analysis was conducted to examine the correlation among parenting style, stressful life events, and children′s behavior problems.Results:Among the 420 participants, boys mainly reported problems of social problems (9/44,20.5%), schizoid disorders (7/44,15.9%) and obsessive-compulsive problems (51/124,41.1%); while girls mainly reported depression (63/98,64.3%) and schizoid disorders (86/154,55.8%). Compared to those with normal CBCL scores, mothers of participants with abnormal scores were more likely to have refusal deny (13.1±5.2 vs. 11.8±3.7) and severe punishment (11.4±4.2 vs. 10.5±3.1) ( t=-3.00, -2.38 both P<0.05). Severe punishment ( OR=1.11 95% CI 1.02-1.22), overprotection by father ( OR=3.41,95% CI 1.34-15.96), and mother preference ( OR=1.74,95% CI 1.08-3.11) were the risk factors for boys′ behavior problems, including somatic complaints, immaturity, and social withdrawal behaviors (all P<0.05), while mother preference ( OR=1.31,95% CI 1.04-1.74), refusal deny ( OR=1.23,95% CI 1.06-1.47) and emotional warmth ( OR=1.04,95% CI 1.01-1.08) were the risk factors for girls′ behavior problems, including somatic complaints, cruelty, and immaturity (all P<0.05). Participants with abnormal CBCL total score tended to report greater problems in total stress, interpersonal relationship, learning pressure, loss, and health adaptation than those with normal CBCL total score, and the difference was statistically significant ( t=3.48, 2.87, 2.90, 3.29, 3.16, respectively, all P<0.05). Learning pressure and loss were risk factors of emotional behavior ( OR=1.10,1.21, respectively, both P<0.05). Conclusion:Negative parenting style and life events may affect the children′s emotional and behavior problems.

Objective:To understand the influence of parenting style and life events on children and adolescents′ emotional behavior problems.Methods:The Achenbach′s Child Behavior Checklist (CBCL), Egma Minnen av Bardndosnauppforstran (EMBU), and Adolescent Self-Rating Life Events Check List (ASLEC) were used to investigate the children′s behavior problems, parenting style, and stressful life events among 420 children and adolescents (aged 6-16 years) in Beijing. Logistic regression analysis was conducted to examine the correlation among parenting style, stressful life events, and children′s behavior problems.Results:Among the 420 participants, boys mainly reported problems of social problems (9/44,20.5%), schizoid disorders (7/44,15.9%) and obsessive-compulsive problems (51/124,41.1%); while girls mainly reported depression (63/98,64.3%) and schizoid disorders (86/154,55.8%). Compared to those with normal CBCL scores, mothers of participants with abnormal scores were more likely to have refusal deny (13.1±5.2 vs. 11.8±3.7) and severe punishment (11.4±4.2 vs. 10.5±3.1) ( t=-3.00, -2.38 both P<0.05). Severe punishment ( OR=1.11 95% CI 1.02-1.22), overprotection by father ( OR=3.41,95% CI 1.34-15.96), and mother preference ( OR=1.74,95% CI 1.08-3.11) were the risk factors for boys′ behavior problems, including somatic complaints, immaturity, and social withdrawal behaviors (all P<0.05), while mother preference ( OR=1.31,95% CI 1.04-1.74), refusal deny ( OR=1.23,95% CI 1.06-1.47) and emotional warmth ( OR=1.04,95% CI 1.01-1.08) were the risk factors for girls′ behavior problems, including somatic complaints, cruelty, and immaturity (all P<0.05). Participants with abnormal CBCL total score tended to report greater problems in total stress, interpersonal relationship, learning pressure, loss, and health adaptation than those with normal CBCL total score, and the difference was statistically significant ( t=3.48, 2.87, 2.90, 3.29, 3.16, respectively, all P<0.05). Learning pressure and loss were risk factors of emotional behavior ( OR=1.10,1.21, respectively, both P<0.05). Conclusion:Negative parenting style and life events may affect the children′s emotional and behavior problems.

Objective: To compare the clinical effects of two different skin preparation methods for infant craniocerebral surgery. Methods: Totally 120 infants who were going to receive craniocerebral surgery were divided into two groups by random number table, 60 cases in the observation group and 60 cases in the control group. The scalp of both groups was cleaned with moisturizing oil every day from 3 days before operation. On 1 day before operation, the observation group used electric shaver to shave off all hair on the head, and then rinsed with warm water. The control group was treated with skin preparation knife to shave all the hair under soap water lubrication and rinse with warm water. The skin injury rate, incision infection rate and pain score of the two groups were evaluated. Results: The incidence of skin injury and incision infection were 0 and 1.7% (1/60) in the observation group, 18.3% (11/60) and 13.3% (8/60) in the control group, respectively. There were significant differences between the two groups (χ²= 12.110, 5.886, all P < 0.01 or 0.05). The median score of pain in the observation group was 0 (Q₁:0, Q₃:0), while 1.5 (Q₁:1, Q₃:2) in the control group, and there were significant differences between the two groups (Z= 3.286, P < 0.01). Conclusion Electric shaver is superior to skin preparation knife in shaving hair of infants. It not only reduces the incidence of head skin injury, incision infection and pain in the process of skin preparation, but also reduces the incidence of incision infection after craniocerebral surgery in infants. It is worth popularizing.

Objective To compare the function recovery of multiple injuries combined with floating knee joint injury and simple knee joint injury,and to analyze the risk factors.Methods A retrospective case control study was conducted to analyze the clinical data of 41 patients with multiple injuries combined with Blake and McBryde Ⅱ A floating knee injury admitted to Xijing Hospital of Air Force Medical University from June 2011 to June 2017.There were 26 males and 15 females,aged 18-76 years,with an average of 34.5 years.There were 25 patients with simple knee joint injury and and 16 patients with multi-joint combined injury involving knee joint and ipsilateral hip joint or ankle joint injury.Surgical fixation was performed in different parts by external fixation,intramedullary nail and plate screw fixation.According to the Kalstr(o)m and Olerud functional evaluation criteria,the excellent and good rate of postoperative functional recovery was compared between the two groups.The surgical fixation methods of the two groups were compared.Logistic regression analysis was performed on the influencing factors of functional recovery.Results The patients were followed up for 1-7 years,with an average of 3 years.The excellent and good rate of overall functional recovery in the two groups was 68％,and the rate was 84％ in simple knee injury group and 44％ in multi-joint combined injury group (P ＜ 0.01).There was no significant difference in the ratio of intramedullary nail and plate screw fixation between the two groups (P ＞ 0.05),while the proportion of the external fixation in the multi-joint combined injury group [31％ (5/16)] was significantly higher than that in the simple knee joint injury group [16％ (4/25)] (P ＜ 0.05).Logistic regression analysis showed that the rate of external fixation was an independent factor affecting the postoperative function (OR =0.15,P ＜ 0.01).Conclusions The postoperative function in multi-joint injury patients is poorer than in the single joint injury patients.The higher rate of using external fixation in multi-joint injury patients is a risk factor.For Blake and McBryde Ⅱ A floating knee injury combined with multi-joint injury,less external fixation should be used,so as to improve the postoperative function.

Nisin is an antimicrobial peptide widely used in food industry. In this study, Nisin A production in Lactococcus lactis ATCC 11454 was improved by overexpression of Nisin A structural gene nisA through introducing a shuttle expression vector pMG36ek-nisA and an integrated vector pDG780-nisA into the host strain. The differences of growth profiles and Nisin A production level between the two obtained genetic engineering strains FMM1/FMM2 and the parent strain were investigated. Our results show that while the growth profile (the growth rate, biomass and pH) of FMM1 was similar to the parent strain, its Nisin A production increased 31%. In contrast, the biomass of FMM2 was notably lower than the parent strain, while its yield of Nisin A enhanced slightly. The transcription level of genes involved in Nisin A biosynthesis in both engineering strains was further detected by RT-PCR. We found that all the 11 Nisin A biosynthetic genes in FMM1 and FMM2 had a higher transcription level than those in the parent strain, and these genes exhibited more significant increasing degree of transcription level in FMM1 which hosted the autonomous replicating nisA gene. These data suggest that expression of nisA may act as a rate-limit factor in Nisin A biosynthesis. In conclusion, this work provides a new method to improve Nisin A production by increasing the transcription level of nisA, paving the way to further large-scale industrial production of Nisin A.
Bacterial Proteins ; genetics ; Genes, Bacterial ; Genetic Engineering ; Genetic Vectors ; Lactococcus lactis ; genetics ; metabolism ; Nisin ; biosynthesis ; genetics ; Transcription, Genetic

Objective To explore the relationship among genetic polymorphism of angiotension Ⅱ type 1 re-ceptor(AT1 R) A1166-C, angiotensin converting enzyme (ACE) insertion/deletion (I/D), aldosterone synthase (CYP11B2)-344T/C and hypertensive disorder complicating pregnancy.Methods Polymerase chain reaction-re-striction fragment length polymorphism (PCR-RFLP) assay was used to detect the genotypes of AT1 R A1166-C ,ACE (I/O) ,CYP11B2 -344T/C in 86 cases of hypertensive disorder complicating pregnancy and 175 cases of normal control.Results There was 18 combined types in hypertensive disorder complicating pregnancy cases and normal control cases.Compared to AT1R-AA + ACE-Ⅱ + CYP11B2-TT, Odds ratios (OR) of AT1R-AA + ACE-DO +CYP11B2-TC,AT1 R-AC + ACE-ID+CYP11B2-TC and AT1R-AC+ACE-DD+CYP11B2-TC are 7.289,5.315 and 5.694 respectively.There was no statistical significance among the others.Conclusion In all 18 kinds of combined types, AT1 R-AA + ACE-DO + CYP11B2-TC,AT1R-AC+ACE-ID+CYP11B2-TC and AT1 R-AC + ACE-DD +CYP11B2-TC might increase the susceptibility of hypertensive disorder complicating pregnancy.It is possible that multigenes are interacted in the etiology of hypertensive disorder complicating pregnancy.