BACKGROUND: Immunotherapies such as adoptive immune cell infusion and immune-modulating agents are widely used for cancer treatment, and the concomitant symptoms, including cytokine release syndrome (CRS) or immune-related adverse events (irAEs), are frequently reported. However, clinical manifestations induced by mismatched donor granulocyte colony-stimulating factor mobilized peripheral blood mononuclear cell (GPBMC) infusion in patients receiving microtransplant (MST) have not yet been well depicted. METHODS: We analyzed 88 cycles of mismatched GPBMC infusion in patients with acute myeloid leukemia receiving MST and 54 cycles of chemotherapy without GPBMC infusion as a comparison. Clinical symptoms and their correlation with clinical features, laboratory findings, and clinical response were explored. RESULTS: Fever (58.0% [51/88]) and chills (43.2% [38/88]) were the significant early-onset symptoms after GPBMC infusion. Patients possessing less human leukocyte antigen-matching loci with the donor or those with unrelated donors experienced more chills (3 [2-5] loci vs. 5 [3-5] loci, P  = 0.043 and 66.7% [12/18] vs. 37.1% [26/70], P  = 0.024). On the other hand, those with decreased CD4 + /CD8 + T-cell ratio developed more fever (0.8 [0.7-1.2] vs. 1.4 [1.1-2.2], P  = 0.007). Multivariable analysis demonstrated that younger patients experienced more fever (odds ratio [OR] = 0.963, 95% confidence interval [CI]: 0.932-0.995, P  = 0.022), while patients with younger donors experienced more chills (OR = 0.915, 95% CI: 0.859-0.975, P  = 0.006). Elevated ultra-sensitive C-reactive protein levels in the absence of cytokine storm were observed following GPBMC infusion, which indicated mild and transient inflammatory response. Although no predictive value of infusion-related syndrome to leukemia burden change was found, the proportion of host pre-treatment activated T cells was positively correlated with leukemia control. CONCLUSIONS Mismatched GPBMC infusion in MST induced unique infusion-related symptoms and laboratory changes, which were associated with donor- or recipient-derived risk factors, with less safety and tolerance concerns than reported CRS or irAEs.
Humans ; Leukocytes, Mononuclear ; Hematopoietic Stem Cell Transplantation/adverse effects* ; Leukemia, Myeloid, Acute/therapy* ; Unrelated Donors ; Granulocyte Colony-Stimulating Factor ; Graft vs Host Disease

Objective:To construct the concept and its conceptual framework of hospital health literacy(HHL) for exploring the HHL promotion mechanism in the country.Methods:Based on the proceduralised grounded theory, twelve middle or senior managers of hospitals were selected for in-depth interviews and three hospitals were selected for field research from July 2021 to February 2022. Open coding, axial coding, and selective coding were used in data analysis, establishing the concept of HHL and its conceptual framework in China.Results:The conceptual framework of HHL was composed of an internal driver mechanism(hospitals improve their health literacy promotion management system, staff-led health literacy promotion, health literacy promoting physical environment construction), and an external driver mechanism(cooperate with external organizations and institutions to conduct health literacy promotion). The concept of HHL in China was derived as follows: the combination of supportive environments and human resources that health care organizations have in place can improve access and understandability of health information and simplify healthcare services to help patients of different health literacy levels more easily obtain, process, and understand health information as well as to make the most of medical services.Conclusions:Hospital health literacy promotion mechanism in China is a synergy between internal and external driver mechanisms.

Objective:To describe the current situation of health promotion and education in medical and health institutions in China, propose targeted improvement strategies and provide inspiration for medical and health institutions to implement the relevant content of the Health China Action.Methods:We searched China National Knowledge Infrastructure (CNKI) , WanFang Data, VIP China Science and Technology Journal Database, PubMed, Embase, Cochrane Library and so on. The retrieval time limit was from January 1, 2000 to December 30, 2021. The search content was the research on the theme of "health promotion and education in medical and health institutions".Results:A total of 20 articles were included. In the research on health promotion and education of medical and health institutions, the studies involved in "organization management" and "health education" were the most ( n=20) . There were 7 studies (35%) related to "smoke-free hospitals", 4 studies (20%) related to "work effect evaluation", and the studies on "healthy environment" was the least (15%, 3/20) . Conclusions:The work experience of attaching importance to organization management and health education, vigorously promoting the construction of smoke-free hospitals and institutions' health environment, and strengthening the effectiveness evaluation of health promotion and education can provide reference for medical and health institutions to implement the relevant content of health promotion in the Health China Action (2019-2030) .

Complying with overall requirements of the government and regulations on public health emergences, as well as the clinical features of diagnosis and treatment of dental illness, this paper refers to previous guidelines and studies on the infection prevention and control in dental diagnosis and treatment in China and foreign countries. Nanjing Stomatological Hospital has implemented the emergency management practices for the prevention and control of novel coronavirus pneumonia (NCP), mainly focusing on the implementation of prevention and control training programs for medical staffs and the infection control projects on the hospital environment. This study could provide reference for rapid response and emergency management for the prevention and control of NCP in the departments of stomatology.

Cell-Free Nucleic Acids ; blood ; Female ; Humans ; Mosaicism ; Placenta ; Pregnancy

Objective? To systematicaLLy review the respiratory training on swaLLowing function and respiratory function among patients with dysphagia so as to provide a reference for rehabiLitation nursing of patients with dysphagia. Methods? From estabLishing the database to 30th ApriL 2018, we retrieved the randomized controLLed triaLs (RCTs) on respiratory training of patients with dysphagia in PubMed, EMBASE, Web of Science, Cochrane CentraL Register of ControLLed TriaLs, CINAHL, Physiotherapy Evidence Database, Chinese BioMedicaL Literature Database (CBM), China NationaL KnowLedge Infrastructure (CNKI) and WanFang Data by computer. The Literatures were screened and quaLity of the incLuded Literatures were evaLuated according to the system evaLuation method of the Cochrane coLLaboration. The RevMan5.3 was used to statisticaL anaLysis. ResuLts? A totaL of 9 Literatures were incLuded, and three of them were with the LeveL A of Literature quaLity and the rest were with the LeveL B. Meta-anaLysis showed that compared with routine dysphagia nursing, respiratory training couLd improve the swaLLowing function evaLuated by water swaLLow test [RR=1.33, 95%CI (1.17, 1.53), P＜ 0.01], forced vitaL capacity (FVC) [MD =0.47, 95%CI(0.11, 0.82), P＜ 0.01], forced expiratory voLume in one second (FEV1) [MD=0.47, 95%CI(0.20,0.74), P＜0.01], peak expiration fLow (PEF) [MD=1.00, 95%CI(0.91, 1.10), P＜0.01]and reduce the score of penetration-aspiration scaLe (PAS) [MD=-1.05, 95%CI (-1.70, -0.41), P＜ 0.01]of patients with dysphagia. ConcLusions? Compared with routine dysphagia nursing, respiratory training couLd improve the swaLLowing function evaLuated by water swaLLow test, FVC, FEV1, PEF and reduce the score of PAS of patients with dysphagia. However, the findings stiLL need be vaLidated by Large sampLe, muLticenter and high quaLity RCTs.

OBJECTIVE: : To investigate the effect of polysaccharides (LBPs) on TLR/NF-κB independent pathway and serum tumor necrosis factor (TNF-α) level in diabetic MyD88-knockout mice. METHODS: : Diabetes was induced by feeding high-fat/high-sugar diet and injection of low-dose streptozotocin in MyD88-knockout mice. The diabetic mice were randomly divided into model group, positive control group and LBPs group. The expressions of TRAM, TRIF, TRAF6, RIP1 and TNF-α mRNA and proteins in mouse peritoneal macrophages were detected by real-time RT-PCR and Western blotting after LBPs treatment for 3 month. Serum TNF-α was determined with ELISA kit. RESULTS: : Real time RT-PCR showed that compared with model group, the relative expressions of and mRNA in macrophages of LBPs group were significantly decreased and expression of was significantly increased (all <0.05). Expression of TRAM, TRIF, TRAF6, RIP1 and TNF-α proteins as well as serum TNF-α level had no significant difference between LBPs group and model group (all >0.05). CONCLUSIONS : LBPs may not inhibit serum TNF-α level through TLR/NF-κB independent pathway.
Animals ; Diabetes Mellitus, Experimental ; Drugs, Chinese Herbal ; pharmacology ; Gene Expression Regulation ; drug effects ; Macrophages, Peritoneal ; drug effects ; Mice ; Mice, Knockout ; Myeloid Differentiation Factor 88 ; genetics ; NF-kappa B ; genetics ; Random Allocation ; Signal Transduction ; drug effects ; Tumor Necrosis Factor-alpha ; blood ; metabolism

Objective To establish an efficient baculovirus-insect cell expression system for the production of human immunodeficiency virus-1 ( HIV-1 ) envelope glycoprotein gp120 and to evaluate the physiochemical properties, antigenicity and immunogenicity of the recombinant protein. Methods The gene encoding HIV-1 NL4-3 gp120 was cloned into the downstream of pH promoter of the baculovirus transfer vec-tor pAcgp67B to construct the recombinant transfer vector pAc-gp120. Expression of the protein of interest was induced in baculovirus-infected High FiveTM insect cells. ELISA, analytical ultracentrifugation and size-exclusion chromatography were carried out to characterize physicochemical properties of the expressed gp120 protein. Immunogenicity of the recombinant gp120 protein was analyzed by HIV neutralization assay after im-munizing BALB/c mice with it. Results The recombinant HIV-1 gp120 protein was successfully obtained from the established insect cell expression system with a purity of more than 90％ and a mean yield of 13 mg/L in four batches. That recombinant HIV-1 gp120 protein was characterized by homogeneity in solution and possessed a good immunoreactivity to neutralizing antibodies and antisera against HIV. Immunogenicity analysis in BALB/c mice demonstrated that the recombinant gp120 protein could induce effective immune re-sponses against HIV-1 NL4-3. Conclusion A simple and scalable approach to obtain homogeneous and im-munogenic HIV-1 gp120 antigen is successfully established, which will promote further investigation of HIV vaccine candidates.

Objective] To sum up Pro. Cheng Zhiqing’s clinical experience in treating hypertension with insomnia in menopause. [Method] Select 3 classical cases of the disease above treated by Pro. Cheng Zhiqing for analysis and discussion, and summarize her differentiation thought and treatment features. [Result] In her view, such disease is mostly concerned with liver, as well as related to heart, kidney, etc., the pathological course concerns mother-organ disorder involving its child-organ, so the therapy shall center on stretching liver, clearing liver, smoothing liver and tonifying liver, also purging child-organ in excess, nourishing mother-organ in deficiency, treating multi-organ in the same time, therefore to get better clinical effect. [Conclusion] For the hypertension with insomnia in menopause, Pro. Cheng Zhiqing is good at grasping disease mechanism, revising famous and recipe decoctions in generations, with close compatibility of medicines, definite cure effect, worth reference.

Objective To construct the recombinant baculovirus co-expressing M1 and]NA genes of influenza H1N1 virus.Methods The M1 and NA genes of influenza virus (A/PR8/8/34) were amplified by PCR and then inserted into the pFastBacdual (pFBD)donor plasmid to construct the recombinant pFBDM1-NA.pFBD-M1-NA was transformed into DH10Bac E.coli competent cells containing bacmid vector to construct the recombinant shuttle vector rBacmid-M1-NA.Sf9 cells were transfected with rBacmid-M1-NA and then the recombinant baculovirus rBac-M1-NA was obtained.The virus titer was tested by plaque formation,M1 and NA genes in genome were identified by PCR,and the expression of M1 and NA gene by IFA.Results PCR result proved that pFBD-M1-NA and rBacmid-M1-NA were constructed correctly.The recombinant baculovirus rBac-M1-NA titer was 1 × 107pfu/ml.IFA analysis showed that M1 and NA genes were expressed in sf9 cells infected with rBac-M1-NA.Conclusion The recombinant baculovirus coexpressing M1 gene and NA gene of infuenza H1 N1 virus was successfully established.