OBJECTIVES: To examine how the glucose transporter SLC2A1 influences the proliferation and migration of lung adenocarcinoma (LUAD) and explore the underlying molecular mechanisms. METHODS: We examined the differential expression of SLC2A1 between normal and LUAD tissues in the TCGA database and its prognostic implications. Immunohistochemistry was used to detect SLC2A1 protein levels in clinical samples of LUAD and adjacent tissues, and the association of SLC2A1 expression levels with clinicopathological features of the patients was analyzed. In PC9 cells with stable SLC2A1 overexpression or knockdown, the effects of SLC2A1 expression level on cell proliferation and migration were assessed using CCK-8 and Transwell assays, and the changes in expressions of ferroptosis- and autophagy-related proteins were measured; the occurrence of ferroptosis was confirmed using ROS and Fe²⁺ fluorescence staining. RESULTS: SLC2A1 expression was significantly higher in LUAD tumor tissues than in normal lung tissues (P<0.05) and was associated with worse pathological parameters and prognosis of the patients (P<0.05). In PC9 cells, SLC2A1 overexpression significantly promoted cell proliferation, invasion and migration, and SLC2A1 knockdown significanty increased cell death and inhibited cell invasion and proliferation. SLC2A1 knockdown caused obvious activation of cell ferroptosis, reduced GPX4 and xCT expressions, and increased intracellular levels of ROS and Fe²⁺. SLC2A1 knockdown also resulted in increased cell autophagy shown by increased LC3B expression, which could be reversed by treatement with 3-MA. CONCLUSIONS High SLC2A1 expression is correlated with poor prognosis of patients with LUAD, and inhibiting SLC2A1 can induce ferroptosis and autophagy of LUAD cells, suggesting the potential of SLC2A1 as a target for LUAD diagnosis and treatment.
Humans ; Ferroptosis/genetics* ; Cell Proliferation ; Adenocarcinoma of Lung/genetics* ; Lung Neoplasms/genetics* ; Cell Line, Tumor ; Cell Movement ; Glucose Transporter Type 1/genetics* ; Prognosis ; Autophagy ; Neoplasm Invasiveness ; Female ; Male

To investigate the value of apolipoprotein B to apolipoprotein AⅠ ratio combined with carotid intimamedia thickness in predicting acute cerebral infarction.Methods Patients with acute large artery atherosclerotic cerebral infarction in the anterior circulation who were hospitalized in the Department of Neurology，The First Affiliated Hospital of Xinjiang Medical University from July 2020 to June 2021 were selected as the case group.Patients with noncardiovascular and cerebrovascular diseases who were hospitalized during the same period were randomly selected as the control group.The clinical data and laboratory results of the two groups were compared，and the risk factors of acute cerebral infarction were analyzed.To explore the predictive value of apolipoprotein B to apolipoprotein AⅠratio combined with carotid intimamedia thickness in the incidence of cerebral infarction.The clinical data and laboratory results of the two groups were compared.The influencing factors of acute cerebral infarction were analyzed by logistic regression.The receiver operating characteristic curve was used to analyze the predictive value of the ratio of apolipoprotein B to apolipoprotein AⅠcombined with carotid intimamedia thickness in the incidence of cerebral infarction.Results The study included 143 patients with acute cerebral infarction and 122 patients with noncardiovascular and cerebrovascular diseases.Multivariate analysis showed that smoking（OR=3.448，95%CI 1.609-7.391），diabetes（OR=2.884，95%CI 1.389-5.989），carotid intimamedia thickness （OR=1.633，95%CI 1.219-2.189），Leukocytes （OR=1.258，95%CI 1.036-1.527），free fatty acids（OR=14.113，95%CI 1.942-102.558），ratio of apolipoprotein B to apolipoprotein AI（OR=10.896，95%CI 2.290-51.846），D Dimer（OR=4.690，95%CI 2.097-10.488），homocysteine（OR=9.594，95%CI 1.112-82.772） were independent risk factors for large atherosclerotic cerebral infarction.The value of the ratio of apolipoprotein B to apolipoprotein AⅠ [JP3]and carotid intimamedia thickness in predicting acute cerebral infarction was analyzed by receiver operating characteristic curve.The results showed that the area under the curve was 0.757（95%CI 0.699-0.816），the best predictive value was 0.466，the sensitivity was 81.8%，and the specificity was 58.2%.Conclusion The ratio of apolipoprotein B to apolipoprotein AⅠ and the carotid intimamedia thickness are related to the atherosclerotic cerebral infarction of the anterior circulation large arteries，and they have a certain value in jointly predicting the incidence of cerebral infarction.

Objective To investigate the relationship between coping resources and the quality of life of paraplegics.Methods Fifty-six paraplegic individuals were recruited to complete a set of self-assessment questionnaires:the Generic Quality of Life Inventory,the Life Events Questionnaire,the Fighting Spirit and Helplessness subscales of the Hopelessness in Mental Adjustment to Cancer Scale and Folkman's Ways of Coping Questionnaire.Their answers were used to assess the impact of paraplegia on the activities of daily living,the patients' understanding about paraplegia and their coping strategies.Stepwise multiple regression analysis was conducted to explore how coping resources might predict quality of life.Results Quality of life was the outcome variable in the multiple regression analysis.Only negative life events and coping resources such as uncontrollable cognitive appraisal (helplessness and hopelessness),coping strategies (wishful thinking and seeking support) and support from social forces proved significant in predicting quality of life.Of all the factors,helplessness and hopelessness played the most important roles.Conclusions Coping resources and life events have a greater impact on the quality of life of paraplegics than paraplegia itself.Uncontrollable cognitive appraisals (helplessness and hopelessness) are the most important.Interventions minimizing uncontrolled cognitive appraisals of their condition will be the most effective in improving the quality of life of paraplegic patients.

ObjectiveTo assess fine needle aspiration (FNAC) in the early diagnosis for auto immune thyroid disease (AITD) in adolescent.Methods Seventy-one patients, age ranging from 9 to 23 years with thyroid enlargement underwent FNAC. Their smears were classified into 4 types according to the degree of degeneration of adenoidal cell and lymphocyte invasion by HE combining with rapid staining.The results were compared with B ultrasound and serum thyroid auto antibody measurement.ResultsB ultrasound showed hyperthyroidism in 12 cases, inflammation in 53, nodule change in 5, normal in 5. Thyroid function examination demonstrated hyperthyroidism in 22 cases, hypothyroidism in 9 cases, and euthyroidism in 40 cases. Auto thyroid antibody (TGAb,TMAb) were all beyond normal range except in 1 case, and TPOAb elevated in 37 cases. FNAC identified Grave′s disease in 14, Hashimoto′s diseases typeⅠ( HT-Ⅰ) in 35 cases,HT(Ⅱ) in 13,HT(Ⅲ) in 3, and HT(Ⅳ) in 6 cases. ConclusionsFNAC contributes to early diagnosis of AITD in adolescent for its safety, simplicity, rapidity and accuracy.