Objective To explore association between serum uric acid(SUA)in early pregnancy and the subsequent risk of preeclampsia during pregnancy.Methods In this cohort study,2875 pregnant women who went to obstetrics department of Renmin Hospital of Wuhan University from January 2017 to December 2021 were retrospectively included.Univariate and mult-ivariate penalized smoothing splines cox regression was applied to model the association of serum UA with risk of preeclamp-sia.Results Non-linear associations between serum UA and preeclampsia risk were observed,and there was a U-shaped associ-ation of uric acid with the risk of preeclampsia in pregnant women.pregnant women had the lowest risk of PE when SUA=282μmol/L.The cox regression result showed that the risk of preeclampsia in pregnant women with SUA levels＜220 μmol/L or≥325 μmol/L in the first trimester was 2.72 times(95％CI:1.83-4.05)or 2.82 times(95％CI:1.88-4.22)than those with uric acid levels of 275-299 μmol/L.Conclusion There is a U-shaped association between serum UA levels in early pregnancy and preeclampsia.Either too high or too low SUA will increase the risk of PE.

Objective:To investigate the correlation between the age of men and the quality of semen, as well as the DNA fragmentation index (DFI) in assisted reproduction couples.Methods:A cross-sectional study was conducted, collecting clinical data from 3 361 men who underwent assisted reproductive technology for fertility treatment at the Reproductive Medicine Center of Wuhan University People's Hospital from January 2020 to August 2023 due to non-male factors. Computer-assisted semen analysis system (CASA) was used to analyze semen quality parameters, and DFI was measured by Sperm Chromatin Structure Assay (SCSA). Patients were categorized into three groups based on age: 20-29 years (group A), 30-39 years (group B), and 40-59 years (group C). Multiple linear regression and restricted cubic spline (RCS) were used to analyze the correlation between age and semen quality parameters as well as DFI.Results:The study included 3 361 participants with an average age of (34.86±5.34) years. In groups B and C, sperm progressive motility [(40.17±18.16)%, (37.83±16.96)%] and total motility [(55.27±21.37)%, (53.09±21.14)%] were significantly lower than those in group A [(43.78±18.16)%, (58.29±20.24)%], sperm concentration [(78.96±63.04)×10 6/mL, (91.93±72.28)×10 6/mL] and DFI [(19.18±12.18)%, (21.73±12.52)%] were significantly higher than those in group A [(71.75±57.44)×10 6/mL, (16.31±10.04)%], semen volume was significantly lower in group C [(2.94±1.42) mL] than in groups B and A [(3.28±1.43) mL, (3.15±1.58) mL], with statistically significant differences (all P<0.05). After adjusting for education level, body mass index, smoking, alcohol consumption, and abstinence days, the results of multiple linear regression showed that compared with group A, the age of group B was negatively associated with sperm progressive motility ( β=-3.055, 95% CI: -4.879--1.231) and total motility ( β=-2.366, 95% CI:-4.516--0.216), while positively associated with sperm concentration ( β=7.752, 95% CI: 1.398-4.106) and DFI ( β=2.744, 95% CI: 1.526-3.961). Compared with group A, the age of group C was negatively correlated with semen volume ( β=-0.379, 95% CI: -0.565--0.192), sperm progressive motility ( β=-5.507, 95% CI: -7.714--3.301), and total motility ( β=-4.932, 95% CI: -7.532--2.331), while positively correlated with sperm concentration ( β=17.288, 95% CI: 9.604-24.973) and DFI ( β=5.226, 95% CI: 3.753-6.699). The results of RCS analysis indicated a significant linear dose-response relationship between age and semen volume ( Pnon-linearity=0.424), showing a decline in semen volume with increasing age ( P<0.001). Non-linear dose-response relationships were observed between age and sperm density ( Pnon-linearity=0.003), sperm progressive motility ( Pnon-linearity<0.001), total motility ( Pnon-linearity<0.001), and DFI ( Pnon-linearity=0.004). Conclusion:In men undergoing assisted reproductive technology for fertility treatment due to non-male factors, age greater than 30 years is significantly associated with a decrease in semen volume, sperm progressive motility, and total motility, as well as an increase in sperm density and DFI.

Objective:To investigate the correlation between the age of men and the quality of semen, as well as the DNA fragmentation index (DFI) in assisted reproduction couples.Methods:A cross-sectional study was conducted, collecting clinical data from 3 361 men who underwent assisted reproductive technology for fertility treatment at the Reproductive Medicine Center of Wuhan University People's Hospital from January 2020 to August 2023 due to non-male factors. Computer-assisted semen analysis system (CASA) was used to analyze semen quality parameters, and DFI was measured by Sperm Chromatin Structure Assay (SCSA). Patients were categorized into three groups based on age: 20-29 years (group A), 30-39 years (group B), and 40-59 years (group C). Multiple linear regression and restricted cubic spline (RCS) were used to analyze the correlation between age and semen quality parameters as well as DFI.Results:The study included 3 361 participants with an average age of (34.86±5.34) years. In groups B and C, sperm progressive motility [(40.17±18.16)%, (37.83±16.96)%] and total motility [(55.27±21.37)%, (53.09±21.14)%] were significantly lower than those in group A [(43.78±18.16)%, (58.29±20.24)%], sperm concentration [(78.96±63.04)×10 6/mL, (91.93±72.28)×10 6/mL] and DFI [(19.18±12.18)%, (21.73±12.52)%] were significantly higher than those in group A [(71.75±57.44)×10 6/mL, (16.31±10.04)%], semen volume was significantly lower in group C [(2.94±1.42) mL] than in groups B and A [(3.28±1.43) mL, (3.15±1.58) mL], with statistically significant differences (all P<0.05). After adjusting for education level, body mass index, smoking, alcohol consumption, and abstinence days, the results of multiple linear regression showed that compared with group A, the age of group B was negatively associated with sperm progressive motility ( β=-3.055, 95% CI: -4.879--1.231) and total motility ( β=-2.366, 95% CI:-4.516--0.216), while positively associated with sperm concentration ( β=7.752, 95% CI: 1.398-4.106) and DFI ( β=2.744, 95% CI: 1.526-3.961). Compared with group A, the age of group C was negatively correlated with semen volume ( β=-0.379, 95% CI: -0.565--0.192), sperm progressive motility ( β=-5.507, 95% CI: -7.714--3.301), and total motility ( β=-4.932, 95% CI: -7.532--2.331), while positively correlated with sperm concentration ( β=17.288, 95% CI: 9.604-24.973) and DFI ( β=5.226, 95% CI: 3.753-6.699). The results of RCS analysis indicated a significant linear dose-response relationship between age and semen volume ( Pnon-linearity=0.424), showing a decline in semen volume with increasing age ( P<0.001). Non-linear dose-response relationships were observed between age and sperm density ( Pnon-linearity=0.003), sperm progressive motility ( Pnon-linearity<0.001), total motility ( Pnon-linearity<0.001), and DFI ( Pnon-linearity=0.004). Conclusion:In men undergoing assisted reproductive technology for fertility treatment due to non-male factors, age greater than 30 years is significantly associated with a decrease in semen volume, sperm progressive motility, and total motility, as well as an increase in sperm density and DFI.