Objective:To investigate the application value of combined detection of urine genes Twist1,Onecut2,VIM methyl-ation and folate metabolism related genes MTHFR(C677T/A1298C),MTRR(A66G)polymorphisms in the screening and diagnosis of bladder cancer.Methods:A total of 134 patients with primary bladder cancer admitted to the Department of Urology of Qingyang Peo-ple's Hospital and the Second Hospital of Lanzhou University from January 2023 to January 2024 were selected(bladder cancer group),and a total of 130 patients with common benign urinary system diseases and other malignant tumors of urinary system treated with cystoscopy were admitted during the same period(control group).Methylation-specific polymerase chain reaction was used to de-tect the methylation of Twist1,Onecut2 and VIM genes in urine shed cells.PCR fluorescence probe fusion was used to detect the poly-morphism of folate metabolism-related genes in peripheral blood of patients,and collected the clinical data and immunological indica-tors,and to all the data for statistical analysis.Results:The methylation rates of hematuria,bladder irritation,Twist1,Onecut2 and VIM genes were significantly different between two groups(P<0.05).The area under ROC curve(AUC)of Twist1,Onecut2 and VIM genes methylation and their combined detection were 0.721,0.675,0.674 and 0.772,respectively.Sensitivity and specificity were 73.20%and 71.00%,56.10%and 79.00%,48.80%and 86.00%,80.50%and 69.00%,respectively.The AUC of hematuria and blad-der irritation were 0.661 and 0.652.The sensitivity and specificity were 60.20%and 72.00%,41.50%and 89.00%,respectively.The combined AUC of all indicators were the largest(0.858),and the sensitivity and specificity were higher.The frequencies of CC,CT,TT,and T alleles of MTHFR C677T in bladder cancer group were 21.64%,41.79%,36.56%and 57.46%,respectively.Compared with the control group,the difference was statistically significant(P<0.05).The T allele frequency was significantly different between methylated and unmethylated Twist1 groups(P<0.05).Others differences were not statistically significant,and there was no signifi-cant association with gene methylation(P>0.05).Conclusion:The methylation of Twist1,Onecut2 and VIM genes are highly ex-pressed in the urine cells of patients with bladder cancer,and the combination of hematuria and bladder irritation has a high predictive value for the diagnosis of bladder cancer.The MTHFR(C677T)T allele is associated with the methylation of Twist1 gene and may be one of the risk factors for bladder cancer.

Objective To investigate the effects of Esketamine(S-KET)on the proliferation,migration,invasion,and Wnt/β-catenin signaling pathway of liver cancer cells.Methods Hep G2 liver cancer cells were separated into Control group,low,medium,and high concentration treatment groups(S-KET-L,S-KET-M,S-KET-H groups),and high concentration treatment group(S-KET-H+LiCl group);Edu was applied to detect cell proliferation;scratch experiment was applied to detect cell migration;Transwell experiment was applied to detect cell invasion.Western blot was applied to detect the expression of nuclear proliferation antigen markers(Ki-67),Cyclin D1,matrix metalloproteinase 2(MMP-2),matrix metalloproteinase 9(MMP-9),Wnt1,and β-catenin proteins;nude mouse transplanted tumor was applied to detect the effect of Esketamine on the growth of liver cancer transplanted tumors;immunohistochemistry was applied to detect the expression of Wnt1 and β-catenin proteins.Results Compared with the Control group,the Edu positive rate,Ki-67,Cyclin D1,scratch healing rate,number of cell invasions,the expression of MMP-2,MMP-9,Wnt1,and β-catenin proteins in Hep G2 cells in the S-KET-L,S-KET-M,and S-KET-H groups were obviously reduced(P＜0.05);compared with the S-KET-H group,the Edu positive rate,Ki-67,Cyclin D1,scratch healing rate,number of cell invasions,the expression of MMP-2,MMP-9,Wnt1,and β-catenin proteins in Hep G2 cells in Hep G2 cells in the S-KET-H+LiCl group were significantly increased(P＜0.05);the results of nude mouse tumor transplantation experiment showed that compared with the Control group,the S-KET group mice showed slow tumor growth,reduced tumor mass and volume,and significantly reduced expression of Wnt1 and β-catenin proteins(P＜0.05).Conclusion Esketamine can inhibit the proliferation,migration,and invasion of liver cancer cells by inhibiting the Wnt/β-catenin signaling pathway.

Objective To investigate the effects of Esketamine(S-KET)on the proliferation,migration,invasion,and Wnt/β-catenin signaling pathway of liver cancer cells.Methods Hep G2 liver cancer cells were separated into Control group,low,medium,and high concentration treatment groups(S-KET-L,S-KET-M,S-KET-H groups),and high concentration treatment group(S-KET-H+LiCl group);Edu was applied to detect cell proliferation;scratch experiment was applied to detect cell migration;Transwell experiment was applied to detect cell invasion.Western blot was applied to detect the expression of nuclear proliferation antigen markers(Ki-67),Cyclin D1,matrix metalloproteinase 2(MMP-2),matrix metalloproteinase 9(MMP-9),Wnt1,and β-catenin proteins;nude mouse transplanted tumor was applied to detect the effect of Esketamine on the growth of liver cancer transplanted tumors;immunohistochemistry was applied to detect the expression of Wnt1 and β-catenin proteins.Results Compared with the Control group,the Edu positive rate,Ki-67,Cyclin D1,scratch healing rate,number of cell invasions,the expression of MMP-2,MMP-9,Wnt1,and β-catenin proteins in Hep G2 cells in the S-KET-L,S-KET-M,and S-KET-H groups were obviously reduced(P＜0.05);compared with the S-KET-H group,the Edu positive rate,Ki-67,Cyclin D1,scratch healing rate,number of cell invasions,the expression of MMP-2,MMP-9,Wnt1,and β-catenin proteins in Hep G2 cells in Hep G2 cells in the S-KET-H+LiCl group were significantly increased(P＜0.05);the results of nude mouse tumor transplantation experiment showed that compared with the Control group,the S-KET group mice showed slow tumor growth,reduced tumor mass and volume,and significantly reduced expression of Wnt1 and β-catenin proteins(P＜0.05).Conclusion Esketamine can inhibit the proliferation,migration,and invasion of liver cancer cells by inhibiting the Wnt/β-catenin signaling pathway.

Objective:To investigate the application value of combined detection of urine genes Twist1,Onecut2,VIM methyl-ation and folate metabolism related genes MTHFR(C677T/A1298C),MTRR(A66G)polymorphisms in the screening and diagnosis of bladder cancer.Methods:A total of 134 patients with primary bladder cancer admitted to the Department of Urology of Qingyang Peo-ple's Hospital and the Second Hospital of Lanzhou University from January 2023 to January 2024 were selected(bladder cancer group),and a total of 130 patients with common benign urinary system diseases and other malignant tumors of urinary system treated with cystoscopy were admitted during the same period(control group).Methylation-specific polymerase chain reaction was used to de-tect the methylation of Twist1,Onecut2 and VIM genes in urine shed cells.PCR fluorescence probe fusion was used to detect the poly-morphism of folate metabolism-related genes in peripheral blood of patients,and collected the clinical data and immunological indica-tors,and to all the data for statistical analysis.Results:The methylation rates of hematuria,bladder irritation,Twist1,Onecut2 and VIM genes were significantly different between two groups(P<0.05).The area under ROC curve(AUC)of Twist1,Onecut2 and VIM genes methylation and their combined detection were 0.721,0.675,0.674 and 0.772,respectively.Sensitivity and specificity were 73.20%and 71.00%,56.10%and 79.00%,48.80%and 86.00%,80.50%and 69.00%,respectively.The AUC of hematuria and blad-der irritation were 0.661 and 0.652.The sensitivity and specificity were 60.20%and 72.00%,41.50%and 89.00%,respectively.The combined AUC of all indicators were the largest(0.858),and the sensitivity and specificity were higher.The frequencies of CC,CT,TT,and T alleles of MTHFR C677T in bladder cancer group were 21.64%,41.79%,36.56%and 57.46%,respectively.Compared with the control group,the difference was statistically significant(P<0.05).The T allele frequency was significantly different between methylated and unmethylated Twist1 groups(P<0.05).Others differences were not statistically significant,and there was no signifi-cant association with gene methylation(P>0.05).Conclusion:The methylation of Twist1,Onecut2 and VIM genes are highly ex-pressed in the urine cells of patients with bladder cancer,and the combination of hematuria and bladder irritation has a high predictive value for the diagnosis of bladder cancer.The MTHFR(C677T)T allele is associated with the methylation of Twist1 gene and may be one of the risk factors for bladder cancer.

Objective:To investigate the clinical effect of preemptive analgesia with pentazocine on perioperative pain management in partial splenectomy.Methods:A total of 100 patients with traumatic splenic rupture who underwent partial splenectomy at Yiwu Central Hospital between October 2019 and November 2021 were randomly assigned to either the control group or the study group, with 50 patients in each group using the random number table method. Both groups received patient-controlled analgesia postoperatively. Additionally, the study group received intravenous pentazocine administration before surgery. The amount of anesthetic used during surgery, postoperative anesthesia recovery indices, postoperative pain response, serum inflammatory factor levels, and the number of effective patient-controlled analgesia pump presses within 48 hours postoperatively were recorded and evaluated. Any adverse drug reactions were also monitored.Results:The dosages of propofol [(462.24 ± 27.13) mg] and remifentanil [(365.98 ± 26.78) μg] in the study group were significantly lower than those in the control group [(511.82 ± 26.32) mg, (406.86 ± 26.08) μg, t = 14.49, 12.63, both P < 0.001). The recovery time of spontaneous breathing [(6.86 ± 0.97) minutes], anesthesia recovery time [(13.24 ± 0.82) minutes] and extubation time [(17.14 ± 1.07) minutes] were significantly shorter than those in the control group [(7.62 ± 0.90) minutes, (14.32 ± 0.84) minutes, (18.22 ± 1.06) minutes, t = 5.80, 8.58, 6.93, all P < 0.001]. The Visual Analogue Scale (VAS) scores in the study group were significantly lower than those in the control group at 24 and 48 hours after surgery, both in resting and coughing state ( t = 7.82, 9.31, 4.95, 8.47, all P < 0.001). The serum levels of tumor necrosis factor-alpha, interleukin-1, and interleukin-6 were significantly lower in the study group than in the control group ( t = 21.53, 25.61, 18.45, 16.90, 17.33, 14.86, all P < 0.001), while the serum level of interleukin-10 was significantly higher in the study group than in the control group ( t = -20.85, -19.61, both P < 0.001). The number of effective patient-controlled pump analgesia presses within 48 hours postoperatively in the study group [(6.24 ± 1.17) times] was significantly lower than that in the control group [(10.26 ± 1.34) times, t = 12.95, P < 0.05). In addition, the overall incidence of adverse drug reactions in the study group [4.00% (2/50)] was significantly lower than that in the control group [18.00% (9/50), χ2 = 5.01, P < 0.05]. Conclusion:Preemptive analgesia with pentazocine for patients undergoing partial splenectomy can effectively reduce the dosage of anesthetics during surgery and the dosage of analgesics after surgery, enhance the recovery from postoperative anesthesia, suppress postoperative inflammatory reactions, alleviate pain responses, and minimize the risk of adverse drug reactions.

Objective To explore the corr-elation between cognitive impairment and event-related potential P300 in patients with acute ischemic stroke-related obstructive sleep apnea(OSA).Methods A total of 114 patients with acute ischemic stroke-related OSA admitted to the Department of Neurology of Cangzhou Hospital of Integrated Traditional Chinese Medicine and Western Medicine of Hebei from January 2022 to August 2023 were selected as the research subjects.Patients with cognitive impairment were included in the observation group(n=38),and patients without cognitive impairment were included in the control group(n=76).A standardized mini-mental state examination(MMSE)was used to test the cognitive function of patients in the two groups,and the MMSE scores of patients in the two groups were compared.The brain regions affected by infarct lesions of patients in the two groups were examined by using cranial magnetic resonance diffusion-weighted imaging and magnetic resonance imaging.The SC-500 non-contact,non-intrusive sleep monitoring sensor mattress was used to screen and assess stroke-related OSA in the two groups.The American Nerosscan 64-channel 10/20 electroencephalographic system was employed to detect event-related potential P300 of patients in the two groups.Univariate and multivariate logistic regression analysis were conducted to examine the correlation between cognitive impairment and event-related potential P300 in patients with acute ischemic stroke-related OSA.Results The total MMSE score and the scores in each dimension,including orientation,immediate memory,attention and calculation,recall ability,and language ability of patients in the observation group were significantly lower than those in the control group(P＜0.05).Age,proportions with a history of diabetes,hyperhomocysteinemia,and apnea-hypopnea index(AHI)of patients in the observation group were significantly higher than those in the control group,while the years of education was significantly lower than that in the control group(P＜0.05).The proportions of patients with infarct lesions located in the frontal lobe,temporal lobe,parietal lobe,occipital lobe,thalamus,basal ganglia,brainstem,and centrum semiovale in the observation group were significantly higher than those in the control group(P＜0.05).The P300 latency in the prefrontal,central,and parietal regions of patients in the observation group was significantly longer than that in the control group(P＜0.05).The P300 amplitude in the central and parietal regions of patients in the observation group was significantly lower than that in the control group(P＜0.05).The results of multivariate logistic regression analysis indicated that the years of education was a protective factor against cognitive impairment in patients with acute ischemic stroke-related OSA(P＜0.05).Thalamic infarction,high AHI,and prolonged P300 latency in the parietal region were independent risk factors for cognitive impairment in patients with acute ischemic stroke-related OSA(P＜0.05).Conclusion Patients with acute ischemic stroke-related OSA are prone to cognitive impairment in multiple domains.The P300 latency in the parietal region is closely associated with cognitive impairment in patients with acute ischemic stroke-related OSA and serves as an independent risk factor for cognitive impairment in these patients.

Organoids are three-dimensional (3D) biological structures constructed in vitro by stem cells, which partially mimic the function of real organs. Brain organoids are an extremely important branch of organoid research. This technology can differentiate pluripotent stem cells into the required cell types in a 3D culture environment, and self-assemble into structural bodies, but it is currently unable to fully replicate the structural and physiological features of the real human brain. The maturity of brain organoids may form consciousness, which poses ethical issues such as determining moral status and informed consent in brain organoids research. This paper elaborated on the research progress and future development direction of brain organoids, and proposed multidimensional governance strategies for ethical issues faced in brain organoids research from the perspectives of ethical principles, ensuring public informed consent, and legal supervision. By exploring the above issues, reference will be provided for formulating ethical principles to guide the research and application of brain organoids in the future.

This paper reviews the status quo and influencing factors of family life quality of the elderly with dementia, mainly from effect of the elderly with dementia on the family at home and abroad, the predictive factors affecting family quality of life and social support system, so as to provide theoretical basis for improving family quality of life of the elderly with dementia in the future.

Objective:To explore the effect of imaginative relaxation training in children with acute appendicitis after surgery.Methods:A total of 60 children with acute appendicitis who underwent laparoscopic surgery and received general anesthesia with laryngeal mask from January to October 2021 in the Second Department of Pediatric Surgery, Linyi People's Hospital, were retrospectively selected as the research subject. The 30 children admitted from January to May 2021 were included in the control group, and the 30 children admitted to from June to October 2021 were included in the observation group. The control group was given routine nursing, and the observation group received imaginative relaxation training on the basis of the control group. The postoperative pain, treatment compliance and parental satisfaction of the two groups of children were compared.Results:The postoperative pain score of the observation group was lower than that of the control group, and the treatment compliance and parental satisfaction were higher than those of the control group, and the differences were statistically significant ( P<0.05) . Conclusions:Imaginative relaxation training on the basis of routine nursing for children with acute appendicitis can effectively promote postoperative recovery, relieve postoperative pain, and improve children's treatment compliance and parents' satisfaction with nursing work.

Objective:To explore the effect of imaginative relaxation training in children with acute appendicitis after surgery.Methods:A total of 60 children with acute appendicitis who underwent laparoscopic surgery and received general anesthesia with laryngeal mask from January to October 2021 in the Second Department of Pediatric Surgery, Linyi People's Hospital, were retrospectively selected as the research subject. The 30 children admitted from January to May 2021 were included in the control group, and the 30 children admitted to from June to October 2021 were included in the observation group. The control group was given routine nursing, and the observation group received imaginative relaxation training on the basis of the control group. The postoperative pain, treatment compliance and parental satisfaction of the two groups of children were compared.Results:The postoperative pain score of the observation group was lower than that of the control group, and the treatment compliance and parental satisfaction were higher than those of the control group, and the differences were statistically significant ( P<0.05) . Conclusions:Imaginative relaxation training on the basis of routine nursing for children with acute appendicitis can effectively promote postoperative recovery, relieve postoperative pain, and improve children's treatment compliance and parents' satisfaction with nursing work.