Peripheral neuropathies are commonly diagnosed in different clinical department of the hospital. The diagnosis is generated by a set of reasonable process based on the manifestations of patients. According to the age of onset, the speed of disease development and the symptoms of peripheral nerve lesions, the peripheral neuropathy is divided into a definite clinical subtype for a particular patient. On this basis, utility of the nerve conduction studies and electromyography is conducted to confirm the anatomical locations of peripheral neuropathy. The etiologic diagnosis is based on anatomical diagnosis of peripheral nerve with a reasonable choice of auxiliary tests, including serological testing, peripheral nerve imaging and biopsy. Genetic tests are chosen for patients with clinical suspective diagnosis of hereditary disease. Finally, therapy evaluation on the basis of etiologic diagnosis is important for forming a treatment plan.

Chondroitin sulfate sodium is a sulphated glycosaminoglycan composed of repeating disaccharide units of D-glucuronic acid and N-acetyl-D-galactosamine,prepared from the cartilage tissue of land or marine animal by a specific extraction and purification process.Chondroitin sulfate sodium is considered to have anti-osteoarthritis effect and many other potential physiological activities.It has broad application prospects and development space in the fields of health food,cosmetics,and drugs.This paper reviews the preparation process of chondroitin sulfate sodium,development and problems of microbial synthesis technology and the research status of anti-osteoarthritis activity based on cells models,animal models and clinical randomized controlled trials(RCT).The limitations of current research are analyzed and corresponding strategies are proposed to provide reference for further standardization and development of chondroitin sulfate sodium.

Objective : To study smile exposure in 20- to 30-year-olds with convex facial profiles and to explore the correlation between smile exposure and other aesthetic indicators, as well as psychological factors. Methods : This study obtained ethical approval from the hospital. After they gave informed consent and portrait authorization, 80 young subjects aged 20-30 with convex facial profiles had their dynamic postural smile and spontaneous laughter recorded. The videos were imported into Photoshop CC software, key frames were selected, and the smile exposure was measured. The three-dimensional information of the subject's face in a resting state was obtained, the relevant aesthetic indicators were measured, and the satisfaction degree of their smile and laughter were evaluated. Correlation analysis was conducted for smile exposure and the relevant aesthetic indicators and subjective psychological evaluation. Results : There were statistically significant differences in smile exposure, smile patterns, relevant aesthetic indicators and subjective psychological evaluation between males and females aged 20 to 30 (P<0.05). There was a large gap between males and females in the average opening degree when laughing, males having 5 times that of females, while the average gingival exposure height of the maxillary central incisor in males was approximately 1/2 of that in females. The average nasolabial angle of males (99.80° ± 7.96°) was larger than that of females (96.26° ± 7.31°) (P<0.05), while the average ratio of upper lip length to the length of the lower 1/3 of the face of males (33.73% ± 2.35%) was less than that of females (38.57% ± 2.76%) (P<0.05). The average psychological score of males (57.75±13.46) was higher than that of females (53.69±17.95) (P<0.05). The ratios of maxillary teeth and gingival exposure to oral fissure in the postural smile were positively correlated with the nasolabial angle. The oral fissure width in spontaneous laughter was negatively correlated with psychological score. Conclusion Convex-faced males and females aged 20 to 30 have different smile exposures and smile patterns. Males tend to smile more openly with a larger opening and less gingival exposure, while females tend to grin with a small opening and more gingival exposure. Smile exposure is positively correlated with nasolabial angle and negatively correlated with smile satisfaction.

Objective:To compare the differences in lung function between sanitation workers and the general population undergoing routine physical examinations, and to analyze the risk factors for restricted airflow and severity of the condition in sanitation workers.Methods:This study is a large cross-sectional study called "Shanxin Respiratory Health Screening for Ten Thousand People". A total of 1 036 sanitation workers (sanitation group) and 6 701 individuals from the general population undergoing routine physical examinations (control group) were selected as the original study subjects from June 2021 to April 2022 (before matching). Both groups underwent pre-bronchodilator lung function tests, and the differences in lung function characteristics between the two groups were compared. The sanitation group also completed a questionnaire survey. Multivariate and ordinal multinomial logistic regression analysis were used to analyze the risk factors for airflow limitation and its severity.Results:A total of 1 027 individuals from the sanitation group and 999 individuals from the control group were included in the study. There were no significant differences in age, gender, height, weight, and body mass index (BMI) between the two groups (all P>0.05). The rate of airflow restriction was significantly higher in the sanitation group compared to the control group (22.88% vs 8.81%, P<0.001). In the sanitation group, there was no statistically significant difference in a self-assessment test for chronic obstructive pulmonary disease (CAT) scores between individuals with airflow restriction (235 cases) and those without airflow restriction (792 cases) [(1.50±2.50) vs (1.15±2.03) points, P=0.084]. There were no statistically significant differences in forced vital capacity (FVC) as a percentage of predicted value (FVC%pred) between the two groups. However, the sanitation group had significantly lower %pred for forced expiratory volume in one second (FEV 1%pred), FVC/FEV 1 ratio (FEV 1/FVC%pred), forced expiratory flow at 50% of FVC (FEF 50%%pred), forced expiratory flow at 75% of FVC (FEF 75%%pred), and maximal mid-expiratory flow (MMEF%pred) compared to the control group (all P<0.05). The rates of abnormal FEF 50%%pred, FEF 75%%pred, and MMEF%pred were significantly higher in the sanitation group compared to the control group (17.62% vs 10.31%, 17.04% vs 10.01%, 27.26% vs 18.41%, all P<0.001). Small airway parameters and the rate of airflow restriction were significantly higher in past and current smokers of the sanitation group compared to never smokers (all P<0.05). Multifactorial analysis showed that high BMI ( OR=0.929, 95% CI: 0.885-0.974) was a protective factor for airflow restriction, while high smoking index was a risk factor ( OR=1.020, 95% CI: 1.011-1.030). Ordered multinomial logistic regression analysis showed that high BMI ( OR=0.925, 95% CI: 0.882-0.971) was a protective factor for the severity of airflow restriction, while high smoking index ( OR=1.020, 95% CI: 1.011-1.029) was a risk factor for the severity of airflow restriction. Conclusions:The incidences of airflow limitation and small airway abnormalities in sanitation workers are higher than that in general physical examination population. High smoking index and low BMI are independent risk factors for airflow limitation and its severity.

Group 2 innate lymphoid cells (ILC2s) are novel innate immune cells. Similar to T cells, ILC2s can secrete Th2 cytokines and initiate and maintain type 2 immune responses, which are also named as cross-border immune cells between innate immunity and adaptive immunity. In recent years, infectious diseases caused by pathogenic viruses have become a global healthcare challenge with the outbreaks of Middle East respiratory syndrome (MERS), severe acute respiratory syndrome (SARS) and novel coronavirus disease 2019 (COVID-19). As a member of the innate lymphocyte family, ILC2s are involved in the development of group 2 inflammatory diseases and can activate immune activity, playing an essential role in the repair of tissue damage and in the body’s defense, which also can participate in the immune response and play an important role in viral immunity when a virus attacks on the body. This study reviews the defensive role of ILC2s in viral infections, providing a theoretical basis for elaborating the role of ILC2s in viral infectious diseases.

Objective To understand the prevalence and risk factors of chronic diseases in Xilinguole League, Inner Mongolia, and its risk factors in 2017, so as to provide evidence for the prevention and treatment of chronic non-communicable diseases. Methods The stratified cluster random sampling method was used to conduct a questionnaire survey on the residents in Xianghuangqi area of Xilin Gol League, Inner Mongolia. Epidate 3.1 was used for data entry, and SPSS 25.0 was used for statistical analysis. Results The average age of the subjects was 46.2 ± 11.2 years old, and the prevalence of chronic diseases was 25.26%. The prevalence of hypertension ranks first at 18.56%; the prevalence of women is slightly higher than that of men (χ²=0.968, p<0.05); the difference in prevalence among different ethnic groups is statistically significant (χ²=1447.730, p<0.01); The prevalence of smokers is higher than that of non-smokers (χ²=7.790, p＜0.05); the prevalence of age increases (χ²_trend=137.214, p＜0.01); the prevalence of higher education shows a downward trend (χ²_trend=84.258, p＜0.01); the prevalence of BMI increases showed an upward trend (χ²_trend=31.956, p＜0.01). Smoking is a risk factor for chronic diseases (OR: 1.33, 95% CI: 1.01~1.76); age is a risk factor for chronic diseases, and the prevalence is significantly higher in the age group >69 (OR: 9.11, 95% CI: 2.78~29.78); Education level is a protective factor for chronic diseases. The prevalence of college degree and above is lower than that of illiteracy (OR: 0.16, 95%CI: 0.07~0.36); residents with BMI> 28 have the highest risk of disease (OR: 2.94, 95%CI: 1.36) ~6.32). Conclusion The prevalence of chronic diseases of residents in Xianghuang Banner of Xilingol League of Inner Mongolia should not be ignored. The prevalence of chronic diseases of Mongolian residents is higher than that of Han people. Therefore, it is necessary to change their living and eating habits and improve their awareness of prevention so as to reduce the prevalence of chronic diseases.

Objective:To investigate the inhibitory effect and molecular mechanism of microRNA-30d (miR-30d) in the process of proliferation, migration and invasion of malignant mesothelioma cell line MSTO-211H.Methods:In April 2017, the human MSTO-211H cells was used to establish miR-30d overexpressed MSTO-211H cell model by transfection of miR-30d mimics. The qRT-PCR was performed to detect the expression level of miR-30d in the cells transfected miR-30d mimics. The effects of miR-30d on the proliferation, apoptosis, migration and invasion of MSTO-211H cells were analyzed by CCK-8 experiment, flow cytometry, cell scratch experiment and Transwell method.Results:After transfection of miR-30d, the expression level of miR-30d in the MSTO-211H+miR-30d cells group was significantly higher than MSTO-211H+miR NC cells group ( P<0.01) . The cell activity of MSTO-211H+miR-30d group (105.13%±2.35%) was significantly lower than MSTO-211H+miR NC cells group (115.40%±1.35%) , and the level of apoptosis (3.97%±0.36%) was significantly higher than MSTO-211H+miR NC cells group (1.47%±0.10%) ( P<0.01) . The relative migration areas at 12 and 24 h of MSTO-211H+miR-30d cells group (9.35±3.16 μm 2 and 58.19±1.82 μm 2) were significantly lower than MSTO-211H+miR NC cells group (54.42 ±5.26 μm 2 and 88.32 ±1.96 μm 2) ( P<0.01) . Compared with the MSTO-211H+miR NC cells group, the numbers of cell migration and cell invasion were reduced in the MSTO-211H+miR-30d cells group ( P<0.01) . Conclusion:miR-30d can regulate the progression of malignant pleural mesothelioma by inhibiting the proliferation, apoptosis, migration and invasion of MSTO-211H cells.

Objective:To investigate the inhibitory effect and molecular mechanism of microRNA-30d (miR-30d) in the process of proliferation, migration and invasion of malignant mesothelioma cell line MSTO-211H.Methods:In April 2017, the human MSTO-211H cells was used to establish miR-30d overexpressed MSTO-211H cell model by transfection of miR-30d mimics. The qRT-PCR was performed to detect the expression level of miR-30d in the cells transfected miR-30d mimics. The effects of miR-30d on the proliferation, apoptosis, migration and invasion of MSTO-211H cells were analyzed by CCK-8 experiment, flow cytometry, cell scratch experiment and Transwell method.Results:After transfection of miR-30d, the expression level of miR-30d in the MSTO-211H+miR-30d cells group was significantly higher than MSTO-211H+miR NC cells group ( P<0.01) . The cell activity of MSTO-211H+miR-30d group (105.13%±2.35%) was significantly lower than MSTO-211H+miR NC cells group (115.40%±1.35%) , and the level of apoptosis (3.97%±0.36%) was significantly higher than MSTO-211H+miR NC cells group (1.47%±0.10%) ( P<0.01) . The relative migration areas at 12 and 24 h of MSTO-211H+miR-30d cells group (9.35±3.16 μm 2 and 58.19±1.82 μm 2) were significantly lower than MSTO-211H+miR NC cells group (54.42 ±5.26 μm 2 and 88.32 ±1.96 μm 2) ( P<0.01) . Compared with the MSTO-211H+miR NC cells group, the numbers of cell migration and cell invasion were reduced in the MSTO-211H+miR-30d cells group ( P<0.01) . Conclusion:miR-30d can regulate the progression of malignant pleural mesothelioma by inhibiting the proliferation, apoptosis, migration and invasion of MSTO-211H cells.

Objective:To synthesize amide functionalized graphene oxide nanoribbons (GONRs-AM) and investigate their adsorption performance for uranium.Methods:Characterization of the synthesized GONRs-AM was made by using scanning electron microscopy (SEM) and Fourier infrared spectroscopy (FT-IR). The effects of solution pH, adsorbent dosage, adsorption time, initial uranium concentration, temperature and ionic strength on the uranium adsorption on GONRs-AM were investigated by using batch adsorption experiments.Results:The maximum adsorption capacity of GONRs-AM for uranium was 294.5 mg/g, and the adsorption process was a spontaneous exothermic process influenced by pH, which was consistent with the quasi-second-order kinetic model and the Langmuir model.Conclusions:As an adsorbent, GONRs-AM can effectively adsorb uranium from aqueous solution and can be used to separate uranium from radioactive wastewater.

Objective:To study the characteristics of thorium adsorption onto graphene oxide aerogel (GO-A).Method:GO-A was prepared using hydrothermal reduction assembly method and characterized using scanning electron microscopy (SEM) and Fourier infrared spectroscopy (FT-IR) to investigate its adsorption performance, including the effects of solution pH, adsorption time, initial thorium concentration, and temperature.Results:The maximum adsorption capacity of thorium onto GO-A was 85.8 mg/g under the experimental conditions of pH 3.0, GO-A 20 mg, solution volume 25 ml, and oscillation for 4 h at 25℃.Conclusions:The preparation method of GO-A has a high thorium adsorption capacity owing to being simple, environmental benign, convenient for solid-liquid separation. This result will provide new method and technologies for the treatment of thorium-containing radioactive wastewater and the pre-treatment of samples for environmental monitoring.