Objective To analyze the relevant risk factors for Chinese adult urinary lithiasis.Methods We searched the PubMed,Embase,Cochrane Library databases,the Chinese Biomedical Literature Database,VIP Database,China Knowledge Network (CNKI) database,Wanfang database by computer.The search was set for all case-control studies on urinary lithiasis with Chinese patients.Two authors independently screened the literature,extracted literature data,and evaluated the quality of the literature.The software Revman 5.3 was used to perform meta-analysis in this study.Results A total of 25 studies in this study were case-control studies,with a total of 8 524 cases of urinary lithiasis and 42 239 cases of non-urinary lithiasis.A total of 9 risk factors of urinary lithiasis were screened:low intake of water [OR =2.64(95％CI 2.00-3.48),P ＜0.001],overweight [OR =2.36(95％ CI 1.31-4.23),P =0.004],tobacco consumption[OR =2.10(95％ CI 1.26-3.48),P =0.004],high salt diet [OR =1.88 (95％ CI 1.21-2.91),P =0.005],alcohol consumption [OR =1.85 (95％ CI 1.57-2.17),P ＜ 0.001],low vegetable consumption [OR =1.64 (95 ％ CI 1.05-2.54),P =0.03]、high protein diet [OR =1.49(95％ CI 1.23-1.82),P ＜0.001],low fruit consumption [OR =1.39(95％ CI 1.26-1.53),P ＜0.001] and tea consumption [OR=1.23(95％CI1.10-1.37),P＜0.001].Conclusions Lowintake of water,overweight,tobacco consumption,high salt diet,alcohol consumption,low vegetable consumption,high protein diet,low fruit consumption and tea consumption could be the risk factors for chinese adult urinary lithiasis.

Objective To investigate the effect of cimetidine on the clonal expansion of TCR V? subfamily of T cells in cord blood after the stimulation by K562 cells in vitro.Methods Cimetidine(1?10-5 mol/L) or K562 cells(1?106/ml) or both of them were respectively cultured with mononuclear cells(MNC) isolated from normal human cord blood for 2 weeks.After the induction,specific cytotoxicity of the proliferated T cells were detected with K562 cells as the target cells.The selective usages and clonal expansion of TCR V? subfa-mily of T cells were analyzed by RT-PCR and genescan technique.Results After induction for 2 weeks,the 3 groups showed the increased cell proliferation,in which specific cytotoxicity of T cells induced by both cimetidine and K562 cells against K562 cells was enhanced significantly compared with the other 2 groups(P

AIM: Humanized-NOD/SCID(hu-NOD/SCID) mouse model was established and the level of immune reconstitution was assessed in this model. METHODS: Mononuclear cells (MNC) and CD34+ cells were isolated or sorted from cord blood(CB). Human CD45, CD19, CD3 markers on cells from NOD/SCID murine peripheral blood(PB), bone marrow(BM), thymus were detected by FCM from 4 to 10 weeks after hematopoietic stem cell transplantation. After 10 weeks, the gene expressions of the human ?2M and RAG2 were detected by RT-PCR in PB or bone marrow of mice model. RESULTS: Human CD45, CD19, CD3 cells populations in PB and BM were found by flow cytometry in mice model transplanted with CD34+ cells or CB MNC from 4 to 10 weeks. The highest positivity of human lymphocytes was at 8 week after transplantation. The levels of human cell engraftment in mice transplanted with CD34+ cells were higher than those in mice transplanted with CB MNC. The mRNA of human ?2M and RAG2 were found by RT-PCR in BM.CONCLUSION: The higher level of human lymphocyte engraftment is established in NOD/SCID mouse model transplanted with CD34+ compared with CB MNC. The maturation of T lymphocytes could be happened in bone marrow of mice model.