The inflammatory microenvironment is closely associated with the initiation and progression of osteoarthritis （OA）， specifically manifesting as macrophage activation， dysregulation of inflammatory cytokines， and redox imbalance. Following an overview of the pathological characteristics of the OA inflammatory microenvironment， this paper reviews the research progress on the mechanism of traditional Chinese medicine （TCM） intervening in OA by modulating the inflammatory microenvironment. It has been found that TCM monomers/active ingredients （such as total alkaloids from Strychnos nux-vomica ， quercetin， triptolide， etc.）， herb pairs （e.g. Angelica pubescens - Gentiana macrophylla ， Carthami Flos-Lycopodii Herba）， and TCM formulas （such as Zhuanggu jianxi formula， Duhuo jisheng decoction and Rongjin niantong formula， etc.） can inhibit macrophage activation， reduce the release of proinflammatory cytokines and the generation of reactive oxygen species by inhibiting multiple signaling pathways， including nuclear factor-κB， Wnt/ β -catenin， and mitogen-activated protein kinase， thereby alleviating the articular inflammatory microenvironment， restoring local joint homeostasis， and slowing the progression of OA.

To explore the clinical value of a multimodal predictive model based on multiparametric magnetic resonance imaging(MRI) radiomics combined with deep learning(DL) features for the preoperative noninvasive assessment of mismatch repair-deficient(MMRd) status in endometrial cancer(EC).

Sepsis refers to a life-threatening organ dysfunction caused by a dysregulated host response to infection,with persistently high morbidity and mortality,posing a significant healthcare burden.As integral components of innate and adaptive immunity,macrophages exhibit high plasticity and can differentiate into distinct phenotypes(M1 pro-inflammatory and M2 anti-inflammatory)in response to various environmental stimuli,playing crucial roles in both the hyperinflammatory phase and late immunosuppressive phase of sepsis.The metabolic profile of M2 macrophages has gradually become a research focus,and it is regulated by a variety of enzymes and signaling pathways,including adenosine 5'-monophosphate-activated protein kinase,peroxisome proliferator-activated receptor and protein kinase RNA-like ER kinase pathways.These pivotal signaling pathways and enzymes can promote the polarization of M2 macrophages and enhance their anti-inflammatory functions by modulating the metabolism of glucose,lipid,and amino acid,thereby conferring protective effects against sepsis and providing new ideas for the targeted treatment.

Gout significantly impacts both physical health and quality of life,while current pharmacological treatments face notable limitations.TCM non-pharmacological therapies have shown promising potential in the management of gout,offering diverse approaches with favorable efficacy.This article summarized the characteristics,clinical efficacy and mechanisms of different TCM non-pharmacological therapies for treating gout.Recent studies suggest that these therapies may be applied across all clinical stages of gout.During the acute phase,they can rapidly reduce joint inflammation and relieve pain.In the intercritical phase,they help prevent recurrence,decrease the frequency of attacks,and shorten episode duration.In the chronic tophaceous phase,they alleviate persistent symptoms,improve joint function,and support minimally invasive tophi removal.TCM non-pharmacological therapies have their own characteristics and good safety,and can be combined for clinical use,providing TCM treatment strategies for gout.

Objective To observe the effects of acupuncture at"Jiaji"on angiogenesis in rats with lumbar muscle aging based on AMPK/SIRT1/PGC-1α signaling pathway;To explore its possible mechanism of improving lumbar muscle aging atrophy.Methods Totally 30 SPF male SD rats were randomly divided into model group,acupuncture group and non-acupoint group,with 10 rats in each group.Subcutaneous injection of D-galactose(300 mg/kg)into the neck and back of rats was used to prepare a subacute lumbar muscle aging rat model.Another 10 rats were selected as the blank group,and an equal amount of normal saline was subcutaneously injected into the neck and back.Intervention began in the 9th week of modeling.The acupuncture group received acupuncture at bilateral L3-L5"Jiaji",while the non-acupoint group received bilateral non-meridian and non-acupoint acupuncture,each time for 30 minutes,once a day,6 times a week,for 4 consecutive weeks.β-galactosidase staining was used to observe the morphology of rat lumbar muscle tissue;TUNEL staining was used to detect apoptosis of lumbar muscle tissue cells;ELISA was used to detect the contents of nitric oxide(NO)and endothelin-1(ET-1)in lumbar muscle tissue;immunofluorescence staining was used to detect CD31 expression and microvascular density in lumbar muscle tissue;Western blot was used to detect protein expressions of p-AMP activated protein kinase(AMPK),silencing information regulatory factor 1(SIRT1)and peroxisome proliferator activated receptor gamma co activator factor 1α(PGC-1α)in lumbar muscle tissue.Results Compared with the blank group,the positive cells of β-galactosidase staining in lumbar muscle tissue of the model group increased significantly,the apoptosis rate significantly increased(P<0.001),the NO content in lumbar muscle tissue significantly decreased(P<0.001),the ET-1 content significantly increased(P<0.001),the expression of CD31 and microvascular density significantly decreased(P<0.001),and the protein expressions of p-AMPK,SIRT1 and PGC-1α significantly decreased(P<0.01,P<0.001).Compared with the model group,the positive cells of β-galactosidase staining in lumbar muscle tissue of rats in the acupuncture group was significantly reduced,the apoptosis rate was significantly decreased(P<0.001),NO content significantly increased(P<0.001),ET-1 content was significantly reduced(P<0.01),CD31 expression and microvascular density significantly increased(P<0.01,P<0.001),and the protein expressions of p-AMPK,SIRT1,PGC-1α significantly increased(P<0.05,P<0.01).Conclusion Acupuncture at"Jiaji"can promote angiogenesis in lumbar skeletal muscle,and then improve the aging atrophy of lumbar muscle,and its mechanism may be related to the activation of AMPK/SIRT1/PGC-1α signaling pathway.

Objective:To develop and validate the stroke recovery phase patient active exercise guidance satisfaction rat-ing scale(SRPPAEGSRS)for stroke recovery patients.Method:Using the self-determination theory as the primary framework,integrated with the Brunnstrom stag-ing and social cognitive theories,an initial scale was developed through literature review,semi-structured inter-views,expert consultations,and a preliminary survey.A convenience sampling of 434 stroke patients in the re-covery phase who visited or were hospitalized in the rehabilitation department of a tertiary hospital in Guizhou province from November 2024 to February 2025 were recruited.Reliability and validity tests were conducted to finalize the scale.Result:A total of 422 valid questionnaires were collected,with an effective response rate of 97.24%.The scale consisted of 2 parts,8 dimensions,and 18 items.Three rounds of expert consultations showed a 100%positive coefficient,Kendall's harmony coefficient>0.5,authority coefficient>0.7,and coefficient of varia-tion<0.280.The critical ratio(CR)for all items in each part and the total scale was>8.The correlation coef-ficients between item scores and total scores for each part and the total scale were>0.6.The item-level con-tent validity index(I-CVI)was>0.8,and the scale-level content validity index(S-CVI)was>0.9.Exploratory factor analysis yielded a cumulative variance contribution rate of 77.895%,with no cross-loadings.Confirmatory factor analysis indicated good model fit(χ2/df=1.250,RMSEA=0.034,CFI=0.935,GFI=0.915).The Cronbach's α coefficient,split-half reliability,and test-retest reliability for each part and the total scale were all>0.8.Conclusion:The scale demonstrated good reliability and validity,enabling systematic assessment stroke patients'needs and acceptance of active exercise guidance during recovery.Additionally,it quantifies patients'satisfac-tion with active exercise guidance through the"need-acceptance"discrepancy and identifies bottlenecks in their active rehabilitation.

Objective To study the latent classification of medical service utilization behaviors among middle-aged and older chronic disease patients,providing a theoretical basis for the allocation of medical resources.Methods Using data from the 2018 China Health and Retirement Longitudinal Study(CHARLS)database,latent class analysis was employed to conduct subgroup analysis on the medical choice preferences of middle-aged and older chronic disease patients.Logistic regression was used to analyze the influence of various factors on medical preference subgroups.Results The medical choice preferences of middle-aged and older chronic disease patients were classified into three latent subgroups:public hospital treatment preference type,non-public hospital treatment preference type,and public hospital consultation preference type.Compared to the public hospital treatment preference type,patients under 60 years old(OR=0.456,P=0.034),those living in rural areas(OR=0.256,P=0.006),and those with smoking habits(OR=3.11,P＜0.001)were more inclined to choose non-public hospitals for treatment.In contrast,patients over 60 years old(OR=1.916,P=0.015),those with an education level of primary school or above(OR=2.595,P=0.004),and those with smoking habits(OR=2.591,P=0.044)tended to choose public hospitals for consultation or physical examinations.Conclusion Currently,private hospitals play an increasingly prominent role in chronic disease treatment.However,the utilization rate of traditional Chinese medicine among middle-aged and older chronic disease pa-tients is relatively low,and health awareness remains weak.It is recommended to strengthen the regulation of private hospitals,enhance the credibility and accessibility of traditional Chinese medicine,and intensify health education in communities to meet the growing health demands of middle-aged and older chronic disease patients.

Gout significantly impacts both physical health and quality of life,while current pharmacological treatments face notable limitations.TCM non-pharmacological therapies have shown promising potential in the management of gout,offering diverse approaches with favorable efficacy.This article summarized the characteristics,clinical efficacy and mechanisms of different TCM non-pharmacological therapies for treating gout.Recent studies suggest that these therapies may be applied across all clinical stages of gout.During the acute phase,they can rapidly reduce joint inflammation and relieve pain.In the intercritical phase,they help prevent recurrence,decrease the frequency of attacks,and shorten episode duration.In the chronic tophaceous phase,they alleviate persistent symptoms,improve joint function,and support minimally invasive tophi removal.TCM non-pharmacological therapies have their own characteristics and good safety,and can be combined for clinical use,providing TCM treatment strategies for gout.

Objective To observe the effects of acupuncture at"Jiaji"on angiogenesis in rats with lumbar muscle aging based on AMPK/SIRT1/PGC-1α signaling pathway;To explore its possible mechanism of improving lumbar muscle aging atrophy.Methods Totally 30 SPF male SD rats were randomly divided into model group,acupuncture group and non-acupoint group,with 10 rats in each group.Subcutaneous injection of D-galactose(300 mg/kg)into the neck and back of rats was used to prepare a subacute lumbar muscle aging rat model.Another 10 rats were selected as the blank group,and an equal amount of normal saline was subcutaneously injected into the neck and back.Intervention began in the 9th week of modeling.The acupuncture group received acupuncture at bilateral L3-L5"Jiaji",while the non-acupoint group received bilateral non-meridian and non-acupoint acupuncture,each time for 30 minutes,once a day,6 times a week,for 4 consecutive weeks.β-galactosidase staining was used to observe the morphology of rat lumbar muscle tissue;TUNEL staining was used to detect apoptosis of lumbar muscle tissue cells;ELISA was used to detect the contents of nitric oxide(NO)and endothelin-1(ET-1)in lumbar muscle tissue;immunofluorescence staining was used to detect CD31 expression and microvascular density in lumbar muscle tissue;Western blot was used to detect protein expressions of p-AMP activated protein kinase(AMPK),silencing information regulatory factor 1(SIRT1)and peroxisome proliferator activated receptor gamma co activator factor 1α(PGC-1α)in lumbar muscle tissue.Results Compared with the blank group,the positive cells of β-galactosidase staining in lumbar muscle tissue of the model group increased significantly,the apoptosis rate significantly increased(P<0.001),the NO content in lumbar muscle tissue significantly decreased(P<0.001),the ET-1 content significantly increased(P<0.001),the expression of CD31 and microvascular density significantly decreased(P<0.001),and the protein expressions of p-AMPK,SIRT1 and PGC-1α significantly decreased(P<0.01,P<0.001).Compared with the model group,the positive cells of β-galactosidase staining in lumbar muscle tissue of rats in the acupuncture group was significantly reduced,the apoptosis rate was significantly decreased(P<0.001),NO content significantly increased(P<0.001),ET-1 content was significantly reduced(P<0.01),CD31 expression and microvascular density significantly increased(P<0.01,P<0.001),and the protein expressions of p-AMPK,SIRT1,PGC-1α significantly increased(P<0.05,P<0.01).Conclusion Acupuncture at"Jiaji"can promote angiogenesis in lumbar skeletal muscle,and then improve the aging atrophy of lumbar muscle,and its mechanism may be related to the activation of AMPK/SIRT1/PGC-1α signaling pathway.

Optical coherence tomography(OCT)based on near-infrared light backscattering or reflection,offers an extremely high resolution up to 10 μm.OCT technology has been widely applied in the field of coronary artery disease,but its application in cerebrovascular disease awaits further exploration.Current studies indicate that OCT holds unique advantages in the diagnosis and treatment of ischemic cerebrovascular diseases,including precise plaque characterization,postoperative stent surveillance,and long-term follow-up management.In cases of hemorrhagic cerebrovascular diseases,OCT can assist in evaluating the morphological characteristics of intracranial aneurysms,rupture risk,treatment efficacy,and recurrence.Furthermore,preliminary studies suggest OCT holds potential application value in evaluation of cerebral venous disorders.Future high-quality clinical studies are warranted to further elucidate OCT's clinical value in cerebrovascular disease management and establish standardized application criteria.