Objective To explore the value of transvaginal real-time three-dimensional hysterosalpingo-contrast sonography unblocked were 2.03±0.67 and 0.76±0.10,respectively,which were significantly lower than those in patients with fallopian tube partial obstruction(2.65±0.58 and 0.83±0.11)and fallopian tube obstruction(2.92±0.55 and 0.90±0.12)(all P<0.05).Logistic regression analysis showed that PSV,PI,and RI were the influencing factors for tubal patency(all P<0.05).The area under the ROC curve values of PSV,PI and RI for diagnosis of tubal patency were 0.741,0.722 and 0.747,respectively(P<0.05).The sensitivity and negative predictive value of RT-3D-HyCoSy combined with CDFI in assessing tubal patency were 97.14%and 98.15%,respectively,which were higher than RT-3D-HyCoSy alone(82.85%and 90.63%)(P>0.05).The specificity and positive predictive value of RT-3D-HyCoSy combined with CDFI in assessing tubal patency were 86.69%and 80.95%,respectively,which were lower than RT-3D-HyCoSy alone(96.08%and 90.63%)(P>0.05).Conclusion RT-3D-HyCoSy combined with CDFI has good application value in evaluating tubal patency in infertile patients and deserves further study.