Objective To evaluate the efficacy of skin traction combined with hyperbaric oxygen (HBO) in the treatment of skin defects in crus.Methods Thirty-six patients with skin defects in crus admitted into the trauma department of Yantaishan hospital from August 2010 to April 2018,were treated with external fixation device and skin tractor produced by the Beijing Orthopedic Research Institute.Skin traction was performed after the margin of skin necrosis was clearly identified,and the external fixation device for skin traction was properly placed.From the first day after surgery,the traction ring or the tractor connecting rod was moved forward 1 mm a day in the direction of skin defect,the procedures of which were divided into 6 times.The patients were randomly divided into 2 groups:the routine group (n =19) and the hyperbaric oxygen group (n =17).The following day after surgery,H BO therapy was implemented.Results Of the 17 patients who received skin traction combined with HBO in the HBO group,there was no case of infection and complications.However,of the 19 patients with routine treatment,there were 4 cases of infection,of which 1 case was seriously infected.Skin traction was momentarily suspended and sensitive antibiotics was applied,and 1 week after treatment,infection was controlled and skin traction continued.In the HBO group,there was 1 case of needle out-cut by Kirschner needle,while there were 7 cases in the routine group,of which 3 cases healed after debridement and suture and other 4 cases were treated with secondary skin traction.Conclusion Skin traction combined with HBO in the treatment of skin defects in crus could significantly reduce the loosening of nails and the rate of needle out-cut,and the rate of soft tissue infection as well.

Objective To evaluate the efficacy of skin traction combined with hyperbaric oxygen (HBO) in the treatment of skin defects in crus.Methods Thirty-six patients with skin defects in crus admitted into the trauma department of Yantaishan hospital from August 2010 to April 2018,were treated with external fixation device and skin tractor produced by the Beijing Orthopedic Research Institute.Skin traction was performed after the margin of skin necrosis was clearly identified,and the external fixation device for skin traction was properly placed.From the first day after surgery,the traction ring or the tractor connecting rod was moved forward 1 mm a day in the direction of skin defect,the procedures of which were divided into 6 times.The patients were randomly divided into 2 groups:the routine group (n =19) and the hyperbaric oxygen group (n =17).The following day after surgery,H BO therapy was implemented.Results Of the 17 patients who received skin traction combined with HBO in the HBO group,there was no case of infection and complications.However,of the 19 patients with routine treatment,there were 4 cases of infection,of which 1 case was seriously infected.Skin traction was momentarily suspended and sensitive antibiotics was applied,and 1 week after treatment,infection was controlled and skin traction continued.In the HBO group,there was 1 case of needle out-cut by Kirschner needle,while there were 7 cases in the routine group,of which 3 cases healed after debridement and suture and other 4 cases were treated with secondary skin traction.Conclusion Skin traction combined with HBO in the treatment of skin defects in crus could significantly reduce the loosening of nails and the rate of needle out-cut,and the rate of soft tissue infection as well.

Objective: To observe the expression of long-chain non-coding RNA (lncRNA) RP3-340N1.2 in breast cancer tissues and its effect on proliferation and migration of breast cancer MCF-7 cells, and to explore the possible mechanism. Methods: 13 pairs of breast cancer tissues and adjacent tissues from breast cancer patients, who underwent radical surgery at the Cancer Center of theAffiliated People’s Hospital of Hubei University of Medicine from Jan. 2017 to Sep. 2017, were collected for this study. qRT-PCR was used to detect the differential expression of RP3-340N1.2 in collected tissue samples and breast cancer cell lines and normal breast epithelial cell line. RP3-340N1.2 plasmid (experimental group) and the negative control plasmid (control group) were transfected into breast cancer MCF-7 cells using Lipofectamine 3000. Cell counting (CCK-8) and Transwell migration assay were used to examine the effect of RP3-340N1.2 over-expression on proliferation and migration of MCF7 cells, the effect of RP3-340N1.2 over-expression on the mRNA expression of miR-134-5p and OPCML was detected by qRT-PCR, and Western blotting was used to detect the expression of OPCML protein. Results: The expression of RP3-340N1.2 in breast cancer tissues was significantly lower than that in adjacent tissues ( P <0.01), and the expression of RP3-340N1.2 in breast cancer cell lines was significantly lower than that in normal breast epithelial cells ( P < 0.01). Up-regulation of RP3-340N1.2 decreased the proliferation and migration of MCF7 cells (all P <0.05). After over-expression of RP3-340N1.2 in MCF7 cells, the expression of miR-134-5p obviously decreased ( P <0.01); moreover, the mRNA and protein expressions of OPCML significantly increased ( P <0.01) while the expressions of cell cycle regulatory proteins (CDK4, Cyclin D2) and cell migration regulatory proteins (Vimentin and N-cadherin) decreased significantly (all P <0.01). Conclusion: RP3-340N1.2 is low expressed in breast cancer tissues and cell lines. Up-regulation of RP3-340N1.2 expression can lead to decreased expression of miR-1345p and increased expression of OPCML gene, thereby inhibiting the proliferation and migration of breast cancer cells.

BACKGROUND: Reducing perioperative blood loss in total hip arthroplasty is a hot topic for joint surgeons. Both intravenous infusion and intra-articular injection of tranexamic acid significantly reduce perioperative blood loss, blood transfusion volume, and need for blood transfusion in patients undergoing total hip arthroplasty. However, differences between the intravenous and intra-articular methods are not clear.OBJECTIVE: To evaluate the effects of these two tranexamic acid administration methods on perioperative blood loss in patients undergoing total hip arthroplasty.METHODS: We are conducting a prospective, single-center, open-label, randomized, controlled clinical trial at the Tengzhou Central People's Hospital, China. Ninety patients undergoing unilateral total hip arthroplasty have been randomized into three groups. In the intravenous infusion group (n=30), 15 mg/kg tranexamic acid diluted in 100 mL physiological saline was infused intravenously at the beginning of surgery and 20 mL of physiological saline was injected intra-articularly after deep fascia suturing. In the intra-articular injection group (n=30), 100 mL of physiological saline was infused intravenously at the beginning of surgery and a mixture of 1.5 g tranexamic acid and 20 mL physiological saline was injected intra-articularly after deep fascia suturing. In the control group (n=30), 100 mL of physiological saline was infused intravenously at the beginning of surgery and 20 mL of physiological saline was injected intra-articularly after deep fascia suturing. The primary outcome is hidden blood loss at 1 and 3 days postoperatively. The secondary outcomes are visible blood loss, need for blood transfusion, and mean blood transfusion volume intraoperatively and on days 1 and 3 postoperatively. Other outcomes are the incidence of adverse reactions and complications within 3 months of surgery. The study protocol has been approved by the Ethics Committee of Tengzhou Central People's Hospital of China, approval number 2015-026. All protocols will be performed in accordance with the Ethical Principles for Medical Research Involving Human Subjects in the Declaration of Helsinki. Written informed consent was provided by each patient and their family members after they indicated that they fully understood the treatment plan.DISCUSSION: This trial was designed in April 2015. Cases were collected in July 2015. Data analysis will be finished in December 2017. This study is designed to investigate the effects of intravenous infusion versus intra-articular injection of tranexamic acid on perioperative blood loss in patients undergoing total hip arthroplasty to determine the more effective mode of administration.

Objective To investigate the effect of diabetes on clinical efficacy of transcatheter arterial chemoembolization (TACE) in the treatment of non-viral hepatitis hepatocellular carcinoma (HCC). Methods Retrospectively analyzed the clinical data of 367 non-hepatitis virus HCC patients treated by TACE, included 153 diabetes mellitus cases (test group) and blood glucose of 214 patients was normal (control group). To assess the treatment effect after 1 month of TACE based on response evaluation criteria in solid tumors, include complete response (CR), partial response (PR), stable disease (SD), progressive disease (PD), and calculate the disease control rate. Through 6 to 75 months follow-up to observed long-term efficacy, record the time to progression (TTP) and overall survival (OS) time. Survival rate were analyzed using Kaplan-Meier method and Log-rank analysis by SPSS 16.0. The single-factor analysis was used to analyze variables which variables that differed were analyzed by Cox regression. Results The disease control rate of test group was 69.9%(107/153) and control group was 74.3%(159/214), the difference was no statistically significant (P=0.125). The median time to progression (mTTP) and median overall survival (mOS) of test group were 10.0 and 15.0 months;and the mTTP and mOS of control group were 14.0 and 19.0 months, the difference were statistically significant (P=0.023 and P= 0.026). Tumor diameter ≥4.5 cm, numbers of tumor ≥3, invasion of blood vessels, α-fetoprotein≥200 μg/L, Eastern Cooperative Oncology Group score and diabetes were risk factors for OS of HCC patients. Conclusion Diabetes is unfavorable factors for overall survival of non-hepatitis HCC tread by TACE.

Objective To investigate expression of slug and E-cadherin in pancreatic cancer tissues and determine the inhibitory effects of anti-Slug, an anti-sense plasmid, on the invasion of pancreatic cancer cell lines in vitro. Methods Slug and E-cadherin protein and mRNA was analyzed by IHP and RT-PCR in 36 cases of pancreatic cancer. Then anti-Slug plasmid was transfected into herin and Slug expression. The inhibitory effects of anti-sense Slug were also detected by Transwell motility assay and Matrigel invasion assay. Results The expression of Slug and mRNA in metastatic pancreatic cancer tissue was higher than that in non-metastatic tissue. E-cadherin and mRNA was lower in metastasis tissues(P＜0.05). The inverse relationships were further observed by transient transfection of anti-Slug into SW1990H4 cells. The downregulated expression of Slug and re-expression of E-cadherin were found. The Slug mRNA levels were 0.985±0.016,0.973±0.014, 0. 554±0. 011 after 0, 48 h of transfection of anti-sense Slug, and that of E-cadherin were 0.120±0.001, 0.360±0.002, 0. 727±0. 006, respectively. The diference was significant between different time points (P＜0.05). The Slug mRNA levels were 0. 206±0.017, 0.968±0.015, and that of E-cadherin were 0. 18±0.002,0.727±0.006 after stable transfection of anti-sense Slug, and control plasmid, respectively. The diference was significant (P＜0.05). The motility activity(393±28, 352±24, 96 ±13 )and the invasion activity (223 ± 69, 202 ± 64, 65 ±19) of1 antisense Slug transfectant cells were significantly decreased as compared with those of control cells (P＜0.05). Conclusions Higher expression of slug and lower expression of E-cadherin is related to the invasion and metastasis in pancreatic cancer. A reverse corelation of E-cadherin and Slug expression exists in pancreatic cancer. Slug is possibly a potential target for cancer gene therapy blocking invasion and metastasis in human pancreatic cancer.

OBJECTIVETo investigate the long-term changes of expanded skin after transplantation.

METHODSThe expanded skin in dogs at 0 week, 3 weeks, 6 weeks, 3 months and 6 months after transplantation was observed through areas, histomorphological, electron microscopic, collagen content, biomechanical, and immunohistochemical methods.

RESULTSThe expanded skin was close to the control groups in all observations.

CONCLUSIONThe biological changes after expansion remain in 3 months. The expanded skin will gradually recover to normal in 3-6 months. It is similar to a wound repair process.

Animals ; Collagen ; analysis ; Dogs ; Female ; Male ; Skin ; chemistry ; ultrastructure ; Skin Transplantation ; Tissue Expansion

Objective To investigate the effect of antigen retrieval by microwave and the expression of CD 44 s and CD 44v6 in human intracranial metastastic tumor and human glioblastomas, and the relationship between the expression of CD 44 adhesion molecules and the invasion or metastasis of these tumors.Methods After antigen retrieval by microwave-0.1%Triton X-100(MW-T) and buffer solution of microwave-citrate salts(MW-CA), CD 44 s and CD 44v6 were used to detect the expression of CD 44 adhesion molecules in 20 normal human brain tissues, 35 human glioblastomas, and 30 intracranial metastastic tumor by microwave-LSAB immunohistochemical staining technique.Results Integral optical density(IOD) value of CD 44 s and CD 44v6 positive cells restored by MW-T and MW-CA was higher significantly than that of without antigen retrieval (P