This paper analyzes the characteristics of highly cited papers published in Chinese Clinical Pharmacology and Therapeutics in the past ten years,in order to explore the influence and re-search value of these papers.Highly cited papers are an important index to evaluate the scientific re-search level of scholars and research institutions,so this study focuses on the characteristics of their number,citation frequency,number of co-authors and regional distribution.The paper uses bibliomet-rics to analyze the publication trend and citations of highly cited papers.The results show that during the observation period,the number of highly cited papers decreases year by year,and there is a signifi-cant correlation between cited frequency and publi-cation time.In addition,the number of co-authors presents a normal distribution,among which the number of co-authors is the largest,indicating that moderate cooperation has a positive effect on the influence of papers.From the perspective of geo-graphical distribution,the author group of the mag-azine is relatively dispersed,covering many regions of the country,among which Anhui,Hunan and Zhejiang have become the main sources of high-quality manuscripts.The finding reflects the maga-zine's wide reach across the country.The results of this study provide empirical support for under-standing the characteristics of highly cited papers and emphasize the importance of research collabo-ration and geographical factors in research publica-tion.Through the analysis of highly cited papers,this paper provides references for subsequent re-searchers in selecting research topics and coopera-tion methods,and also provides beneficial sugges-tions for improving the research level and influence of clinical pharmacology in China.

This paper analyzes the characteristics of highly cited papers published in Chinese Clinical Pharmacology and Therapeutics in the past ten years,in order to explore the influence and re-search value of these papers.Highly cited papers are an important index to evaluate the scientific re-search level of scholars and research institutions,so this study focuses on the characteristics of their number,citation frequency,number of co-authors and regional distribution.The paper uses bibliomet-rics to analyze the publication trend and citations of highly cited papers.The results show that during the observation period,the number of highly cited papers decreases year by year,and there is a signifi-cant correlation between cited frequency and publi-cation time.In addition,the number of co-authors presents a normal distribution,among which the number of co-authors is the largest,indicating that moderate cooperation has a positive effect on the influence of papers.From the perspective of geo-graphical distribution,the author group of the mag-azine is relatively dispersed,covering many regions of the country,among which Anhui,Hunan and Zhejiang have become the main sources of high-quality manuscripts.The finding reflects the maga-zine's wide reach across the country.The results of this study provide empirical support for under-standing the characteristics of highly cited papers and emphasize the importance of research collabo-ration and geographical factors in research publica-tion.Through the analysis of highly cited papers,this paper provides references for subsequent re-searchers in selecting research topics and coopera-tion methods,and also provides beneficial sugges-tions for improving the research level and influence of clinical pharmacology in China.

Objective To study on the expression of the eukaryotic recombinant vector carrying HlyX gene of Leptospira serovar Lai in mammalian cell and explore the humoral immune response in BALB/c mice immunized with the recombinant plasmid. Methods The HlyX gene was amplified from Leptospira serovar Lai genomic DNA by PCR and inserted into pcDNA3.1 vector. After transformed into E. coli DH5α,the recombinant plasmid was assayed for identification by PCR analysis,restriction nuclease enzyme digestion and sequencing. The recombinant plasmid was transfected into COS-7 cells,then RT-PCR and Western blot were performed to test the expression of the target gene. The recombinant plasmid was injected intramuscularly into BALB/c mice for three times at intervals of two weeks,and the antibody titer was measured by ELISA. Results PCR showed the full length HlyX gene was about 1100 bp. PCR analysis,restriction nuclease enzyme digestion and sequencing indicated the recombinant vector was constructed successfully. After the plasmid Was transfected into COS-7 cells,a fragment about 1100 bp was found by RT-PCR and a specific band relative molecular mass(Mr)about 40×103,which was consistent with the expected size of the target proteins was showed by Western blot. ELISA showed the antibody titer in BALB/c mice immunized by the HlyX gene of Leptospira serovar Lai can elicit high-titer antibody in BALB/c mice,which has laid the foundation for the application of the DNA vaccine.