Objective: To explore the epidemiological characteristics of injury among middle school students in Zhongshan city, Guangdong Province, and to provide evidence for appropriate student injury prevention strategies and injury monitoring programs. Methods: The cluster random sampling method was used to collect injury information among 2 212 middle school students in urban and suburban areas of Zhongshan city. SPSS 22.0 was used for statistical analysis. Results: Among all the 2 212 middle school students, the incidence of injury was 17.95%. The incidence of injuries (20.9%)and mutiple injuries(6.67%)among boys was higher than that of girls(15.42%,4.61%),and the differences were of statistical significance(χ2=11.21,4.45,P<0.05). The incidence of multiple injuries among junior high school students(6.70%) was higher than that among high school students(4.48%)(χ2=5.20，P<0.05). The injury occurred in the school (63.76%) was higher than that occurred outside of the school (36.24%). Most injures were more likely to occur in sports area in school(33.16%),and most injuries were found occured while doing physical activities(38.01%). Conclusion The prevention of injury among middle school students should focus on junior high school students and male students. School-based injury prevention and health education should focus on campus sports-related injury.

Objective To study the expression of miRNA-219 and γCaMK Ⅱ mRNA in the peripheral blood of the first-episode major depressive disorder (MDD) patients,and explore the relationship between these molecular level and MDD symptom.Methods Fourty-one first-episode major depressive disorder patients diagnosed with DSM-5 criteria and 31 heahy volunteers were included.The blood was collected in the fasting state and the expression levels of miRNA-219 and γCaMK Ⅱ mRNA was detected.The patients' severity of symptoms was assessed with HAMD17.Real-time PCR was used to detect the levels of miRNA-219 and γCaMK Ⅱ mRNA in peripheral blood leukocytes.The correlation between miRNA-219,γCaMK Ⅱ mRNA and HAMD17 was analyzed.Results The miRNA-219 levels of MDD group was significantly lower than that of control group (0.91 ±0.21 vs.1.80±0.24,t=2.753,P=0.0075) in peripheral blood,and the γCaMK Ⅱ mRNA levels of MDD group was significantly higher than that of control group (5.55±0.57 vs.2.16±0.20,t=4.970,P＜0.01).There was the positive correlation between γCaMK Ⅱ mRNA levels and the HAMD17 score of MDD (r=0.460,P=0.0025).Conclusion The levels of miRNA-219 in peripheral blood of MDD patients decreased,and the levels of γCaMK Ⅱ mRNA increased,suggesting that miRNA-219 and γCaMK Ⅱ may be related to the pathogenesis of depression.

Objective To study the expression of miRNA-219 and γCaMK Ⅱ mRNA in the peripheral blood of the first-episode major depressive disorder (MDD) patients,and explore the relationship between these molecular level and MDD symptom.Methods Fourty-one first-episode major depressive disorder patients diagnosed with DSM-5 criteria and 31 heahy volunteers were included.The blood was collected in the fasting state and the expression levels of miRNA-219 and γCaMK Ⅱ mRNA was detected.The patients' severity of symptoms was assessed with HAMD17.Real-time PCR was used to detect the levels of miRNA-219 and γCaMK Ⅱ mRNA in peripheral blood leukocytes.The correlation between miRNA-219,γCaMK Ⅱ mRNA and HAMD17 was analyzed.Results The miRNA-219 levels of MDD group was significantly lower than that of control group (0.91 ±0.21 vs.1.80±0.24,t=2.753,P=0.0075) in peripheral blood,and the γCaMK Ⅱ mRNA levels of MDD group was significantly higher than that of control group (5.55±0.57 vs.2.16±0.20,t=4.970,P＜0.01).There was the positive correlation between γCaMK Ⅱ mRNA levels and the HAMD17 score of MDD (r=0.460,P=0.0025).Conclusion The levels of miRNA-219 in peripheral blood of MDD patients decreased,and the levels of γCaMK Ⅱ mRNA increased,suggesting that miRNA-219 and γCaMK Ⅱ may be related to the pathogenesis of depression.

OBJECTIVETo compare the difference between acupuncture and estazolam on arousal state in patients of primary insomnia, and to explore its nerve electrophysiology mechanism.

METHODSSixty-four patients of primary insomnia were randomized into an acupuncture group (32 cases) and a medication group (32 cases). After 3 patients were excluded, 31 cases in the acupuncture group and 30 cases in the medication group were included. Patients in the acupuncture group were treated with acupuncture at Sishencong (EX-HN 1), Anmian (Extra), Shenmen (HT 7), Sanyinjiao (SP 6), Zhaohai (KI 6), Shenmai (BL 62) as main acupoints, combined with supporting acupoints, once a day, five times per week, continuously for 4 weeks. Patients in the medication group were treated with oral administration of estazolam, once a day, continuously for 4 weeks. The Pittsburgh sleep quality index (PSQI) and mean sleep latency (MSL) of multiple sleep latency test (MSLT) were compared before and after treatment in the two groups; the polysomnography (PSG) was applied to monitor the indices regarding sleep structure.

RESULTSCompared before treatment, PSQI score was reduced after treatment in the two groups (both<0.01), which was more significant in the acupuncture group (<0.05). Compared before treatment, sleep onset latency (SOL), number of awakenings (NWAK) and wake after sleep onset (WASO) were reduced, while total sleep time (TST) and sleep efficiency (SE) were significantly increased in the two groups after treatment (all<0.01). Compared before treatment, the percentage of non-rapid eye movement period 1/2/3 (N1, N2, N3) and the percentage of rapid eye movement period (REM) to TST were not significantly changed after treatment in the medication group (all>0.05). Compared before treatment, the percentage of N1, N2 to TST was reduced, while the percentage of N3 and REM to TST was increased after treatment in the acupuncture group (<0.01). The SOL, NWAK, WASO, TST, SE were not statistically changed after treatment in each group (all>0.05). Compared with the medication group, the percentage of N1 and N2 was reduced while that of N3 and REM was increased after treatment in the acupuncture group (all<0.01). After treatment, MSL of MSLT were obviously decreased in the two groups (both<0.01), which were more significant in the acupuncture group (<0.05).

CONCLUSIONSAcupuncture can more effectively improve sleep quality of primary insomnia than estazolam, and is more beneficial for regulation of hyperarousal state.