Objective To systematically understand the current situation of personnel disputes and put forward tar-geted opinions and suggestions,providing theoretical basis and data support for future research on personnel dis-putes in public hospitals.Methods Based on grounded theory and using NVivo 12 software,it makes a qualitative study on 374 second-instance judgments of personnel disputes in public hospitals retrieved from China Judgment Document Network.Results Through three-level coding of the judgments,found that personnel disputes in public hospitals had characteristic rules in three core nodes:the focus of the dispute(437 reference points),the applica-ble laws and regulations(1 005 reference points),and the winning party(374 reference points),among which the focus of the dispute was mostly the performance and termination of the employment contract.The applicable laws and regulations were mostly general laws and regulations,while the exclusive laws and regulations on personnel were lacking,and the winning parties were mostly hospitals.Conclusion Public hospitals can prevent and reduce the occurrence of personnel disputes from four aspects:strengthening the management of employment contracts,ful-ly fulfilling the obligation of informing establishing an in-hospital mediation mechanism and actively responding to dis-putes.

OBJECTIVE To explore the medication rule and potential mechanism of anti-aging traditional Chinese medicine health care prescription by using data mining,network pharmacology and molecular docking technology.METHODS By searching the special food information query platform of the State Administration for Market Regulation and Yaozhi.com,the health care prescrip-tions with traditional Chinese medicine as the main component and anti-aging effect were searched.The data mining technology was used to analyze the prescription rules,and the core anti-aging traditional Chinese medicine was selected according to the analysis re-sults.The chemical constituents and corresponding targets of core anti-aging traditional Chinese medicines were obtained by searching TCMSP and other databases;potential targets related to aging from GeneCards and OMIM databases were screened;the intersection tar-gets of core Chinese medicine and aging were obtained.Subsequently,protein-protein interaction(PPI)network analysis,gene ontolo-gy(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis were performed using STRING database and Cytoscape 3.7.2 software.Finally,the affinity of core targets and key components was verified by molecular docking technology.RESULTS A total of 244 kinds of anti-aging traditional Chinese medicine health care prescriptions were screened and included.The prescription drugs were mainly tonic drugs,the drug properties were mostly warm and mild,the drug taste was mainly sweet,the drugs were mainly distributed in the five zang organs,with the kidney meridian being the first target.According to the re-sults of comprehensive data analysis,five core Chinese medicines(Wolfberry,ginseng,American ginseng,Astragalus,Poria)were screened out.A total of 105 active ingredients(corresponding to 219 target genes),1 568 aging-related potential targets,and 137 in-tersection targets were obtained.According to the degree value,12 core targets were screened out,mainly involving cancer pathways,lipids and atherosclerosis,AGE-RAGE and other signaling pathways.The results of molecular docking showed that the key anti-aging components could spontaneously and stably bind to the core targets.CONCLUSION Through data mining,network pharmacology and molecular docking,the prescription rules of anti-aging Chinese medicine health care prescriptions are analyzed,and the anti-ag-ing potential targets and mechanism pathways of five core traditional Chinese medicines are preliminarily revealed,so as to provide ref-erence and theoretical basis for delaying aging and preventing and treating related diseases in the elderly.

OBJECTIVE To explore the medication rule and potential mechanism of anti-aging traditional Chinese medicine health care prescription by using data mining,network pharmacology and molecular docking technology.METHODS By searching the special food information query platform of the State Administration for Market Regulation and Yaozhi.com,the health care prescrip-tions with traditional Chinese medicine as the main component and anti-aging effect were searched.The data mining technology was used to analyze the prescription rules,and the core anti-aging traditional Chinese medicine was selected according to the analysis re-sults.The chemical constituents and corresponding targets of core anti-aging traditional Chinese medicines were obtained by searching TCMSP and other databases;potential targets related to aging from GeneCards and OMIM databases were screened;the intersection tar-gets of core Chinese medicine and aging were obtained.Subsequently,protein-protein interaction(PPI)network analysis,gene ontolo-gy(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis were performed using STRING database and Cytoscape 3.7.2 software.Finally,the affinity of core targets and key components was verified by molecular docking technology.RESULTS A total of 244 kinds of anti-aging traditional Chinese medicine health care prescriptions were screened and included.The prescription drugs were mainly tonic drugs,the drug properties were mostly warm and mild,the drug taste was mainly sweet,the drugs were mainly distributed in the five zang organs,with the kidney meridian being the first target.According to the re-sults of comprehensive data analysis,five core Chinese medicines(Wolfberry,ginseng,American ginseng,Astragalus,Poria)were screened out.A total of 105 active ingredients(corresponding to 219 target genes),1 568 aging-related potential targets,and 137 in-tersection targets were obtained.According to the degree value,12 core targets were screened out,mainly involving cancer pathways,lipids and atherosclerosis,AGE-RAGE and other signaling pathways.The results of molecular docking showed that the key anti-aging components could spontaneously and stably bind to the core targets.CONCLUSION Through data mining,network pharmacology and molecular docking,the prescription rules of anti-aging Chinese medicine health care prescriptions are analyzed,and the anti-ag-ing potential targets and mechanism pathways of five core traditional Chinese medicines are preliminarily revealed,so as to provide ref-erence and theoretical basis for delaying aging and preventing and treating related diseases in the elderly.

Objective To systematically understand the current situation of personnel disputes and put forward tar-geted opinions and suggestions,providing theoretical basis and data support for future research on personnel dis-putes in public hospitals.Methods Based on grounded theory and using NVivo 12 software,it makes a qualitative study on 374 second-instance judgments of personnel disputes in public hospitals retrieved from China Judgment Document Network.Results Through three-level coding of the judgments,found that personnel disputes in public hospitals had characteristic rules in three core nodes:the focus of the dispute(437 reference points),the applica-ble laws and regulations(1 005 reference points),and the winning party(374 reference points),among which the focus of the dispute was mostly the performance and termination of the employment contract.The applicable laws and regulations were mostly general laws and regulations,while the exclusive laws and regulations on personnel were lacking,and the winning parties were mostly hospitals.Conclusion Public hospitals can prevent and reduce the occurrence of personnel disputes from four aspects:strengthening the management of employment contracts,ful-ly fulfilling the obligation of informing establishing an in-hospital mediation mechanism and actively responding to dis-putes.

Balancing the risks and benefits of organophosphate pesticides(OPs)on human and environmental health relies partly on their accurate measurement.A highly sensitive fluorescence anti-quenching multi-residue bio-barcode immunoassay was developed to detect OPs(triazophos,parathion,and chlorpyrifos)in apples,turnips,cabbages,and rice.Gold nanoparticles were functionalized with monoclonal antibodies against the tested OPs.DNA oligonucleotides were complementarily hybridized with an RNA fluorescent label for signal amplification.The detection signals were generated by DNA-RNA hybridization and ribonuclease H dissociation of the fluorophore.The resulting fluorescence signal en-ables multiplexed quantification of triazophos,parathion,and chlorpyrifos residues over the concen-tration range of 0.01-25,0.01-50,and 0.1-50 ng/mL with limits of detection of 0.014,0.011,and 0.126 ng/mL,respectively.The mean recovery ranged between 80.3％and 110.8％with relative standard deviations of 7.3％-17.6％,which correlate well with results obtained by liquid chromatography-tandem mass spectrometry(LC-MS/MS).The proposed bio-barcode immunoassay is stable,reproducible and reliable,and is able to detect low residual levels of multi-residue OPs in agricultural products.

Objective To explore the histological changes of the wrist interosseous ligaments after radiofrequency electrothermal shrinkage. Methods Six frozen fresh male adult cadaver wrist ligaments were exploited for the research. The ligaments of the right wrists were treated with radiofrequency electrothermal shrinkage with Arthrocare system, while the ligaments of the left wrists were kept as the normal control. The bone-ligament-hone samples of all the scapholunate (SL) and lunotriquetral (LT) ligaments were prepared, sectioned and then stained with the regular HE staining, toluidine blue staining, Sirius-red staining and immunohistochemistry staining of collagen Ⅲ. The image analysis software was used to compare the staining results. Results The histological structures of SL dorsal ligaments (SL-d) and LT volar ligaments (LT-v) were very similar, and the structures of SL volar ligaments (SL-v) and LT dorsal ligaments (LT-d) were also very similar. The membrane parts of both SL and LT ligaments showed the fibrous cartilage structure. The histological structures of SL-d and LT-v were much less destroyed by the radiofrequency than those of SL-v and LT-d. After radiofrequency electrothermal shrinkage, only the distribution areas of collagen Ⅰ and collagen Ⅲ were significantly changed in the membrane parts of SL and LT ligaments. Conclusion Radiofrequency electrothermal shrinkage treatment can cause minor structural damage to the collagen-dominant ligaments such as SL-d and LT-v, while it can lead to quite severe structural damage to the ligaments containing collagen and lots of loose connective tissue, such as SL-v and LT-d.

To explore the clinical classification of hamate hook fracture and the treatment strategy for different type of fractures, 12 patients who suffered from hamate hook fractures were followed up retrospectively. According to the fracture sites and the prognosis, we classified the hamate hook fractures into 3 types. Type I referred to an avulsion fracture at the tip of hamate hook, type II was a fracture in the middle part of hamate hook, and type III represented a fracture at the base of hamate hook. By the classification, in our series, only 1 fell into type I, 7 type II, and 4 type III. The results were evaluated with respect to the functional recovery, recovery time and the association among the clinical classification, pre-operative complications and treatment results. The average follow-up time of this group was 8.4±3.9 months. Two cases were found to have fracture non-union and both of them were type II fractures. Six patients had complications before operation. Five cases were type II fractures and 1 case type III fracture. All the patients were satisfied with the results at the time of the last follow-up. Their pain scale and grip strength improved significantly after treatment. All the pre-operative complications were relieved. The recovery time of hamate hook excision was significantly shorter than that of the other two treatments. The incidences of both pre-operative complications and non-union in type II fractures were higher than those in type I and type III fractures. It was concluded that, generally, the treatment effects with hamate hook fracture are quite good. The complication incidence and prognosis of the fracture are closely related to the clinical classification. Early intervention is critical for type II fractures.
Adolescent ; Adult ; Follow-Up Studies ; Fractures, Bone ; classification ; surgery ; therapy ; Hamate Bone ; injuries ; surgery ; Humans ; Male ; Retrospective Studies ; Young Adult

To explore the clinical classification of hamate hook fracture and the treatment strategy for different type of fractures, 12 patients who suffered from hamate hook fractures were followed up retrospectively. According to the fracture sites and the prognosis, we classified the hamate hook fractures into 3 types. Type I referred to an avulsion fracture at the tip of hamate hook, type II was a fracture in the middle part of hamate hook, and type III represented a fracture at the base of hamate hook. By the classification, in our series, only 1 fell into type I, 7 type II, and 4 type III. The results were evaluated with respect to the functional recovery, recovery time and the association among the clinical classification, pre-operative complications and treatment results. The average follow-up time of this group was 8.4±3.9 months. Two cases were found to have fracture non-union and both of them were type II fractures. Six patients had complications before operation. Five cases were type II fractures and 1 case type III fracture. All the patients were satisfied with the results at the time of the last follow-up. Their pain scale and grip strength improved significantly after treatment. All the pre-operative complications were relieved. The recovery time of hamate hook excision was significantly shorter than that of the other two treatments. The incidences of both pre-operative complications and non-union in type II fractures were higher than those in type I and type III fractures. It was concluded that, generally, the treatment effects with hamate hook fracture are quite good. The complication incidence and prognosis of the fracture are closely related to the clinical classification. Early intervention is critical for type II fractures.