Enhancing the proactive risk management function of the Rapid Response System(RRS)can effectively im-prove its critical care capabilities.Panyu Hospital of Traditional Chinese Medicine of Guangzhou(Panyu Hospital Guangzhou University of Chinese Medicine)has established the ABCDE-RRS system for critical care,which stands for Able(enhanced post capacity),Broad(diverse quality control),Comprehensive(full participation),Deep(continuous talent cultivation and system optimization),and Early(proactive risk management).This system ensures that the number of critically ill hospitalized patients continues to grow(an increase of 8.99％in the first three quarters of 2024 compared to the same period last year,P＜0.05)while effectively reducing the all-cause mortality rate of hospitalized patients(a decrease of 0.05％in the first three quarters of 2024 compared to the same period last year,P＜0.05).It also enhances the safety culture awareness among all staff(an in-crease of 39.05％after implementation,P＜0.05)and improves patient satisfaction with the hospital(the comprehensive patient evaluation ranking of public hospitals in Guangzhou improved from 17th to 12th place).By establishing an effective operational model for ABCDE-RRS and a tiered talent training model for RRS,this provides a replicable template for the construction of RRS in primary hospitals.

Enhancing the proactive risk management function of the Rapid Response System(RRS)can effectively im-prove its critical care capabilities.Panyu Hospital of Traditional Chinese Medicine of Guangzhou(Panyu Hospital Guangzhou University of Chinese Medicine)has established the ABCDE-RRS system for critical care,which stands for Able(enhanced post capacity),Broad(diverse quality control),Comprehensive(full participation),Deep(continuous talent cultivation and system optimization),and Early(proactive risk management).This system ensures that the number of critically ill hospitalized patients continues to grow(an increase of 8.99％in the first three quarters of 2024 compared to the same period last year,P＜0.05)while effectively reducing the all-cause mortality rate of hospitalized patients(a decrease of 0.05％in the first three quarters of 2024 compared to the same period last year,P＜0.05).It also enhances the safety culture awareness among all staff(an in-crease of 39.05％after implementation,P＜0.05)and improves patient satisfaction with the hospital(the comprehensive patient evaluation ranking of public hospitals in Guangzhou improved from 17th to 12th place).By establishing an effective operational model for ABCDE-RRS and a tiered talent training model for RRS,this provides a replicable template for the construction of RRS in primary hospitals.

Objective To observe erythropoietin (EPO) and its mRNA expression changes in rats cortical neurons when suffering hypoxia and investigate the endogenous EPO protective effect of hypoxia neuronal cells.Methods Cultured cortical neurons were prepared from hypoxia rats and divided into control,hypoxia 12,24,48,72 h group.Using immunohistochemistry,reverse transcriptase polymerase chain reaction (RT-PCR) and Western blot,we observed EPO and its mRNA expression in cells,and also observe the culture medium containing lactate dehydrogenase to evaluate the activity of neurons in the whole process.Results Immunohistochemistry,RT-PCR and Western blot analysis showed:the EPO (20.79 ± 2.98) and its mRNA (0.78 ± 0.05) at 12 h had a basic expression in hypoxia group,compared with the control group (EPO:17.12 ± 1.99; mRNA:0.39 ± 0.05),and the difference was statistically significant (t =2.51,P ＜ 0.05 ; t =13.51,P ＜ 0.01) ; the strongest expression was observed at 48 h (EPO:28.88 ± 3.41,mRNA:1.45 ± 0.07),the difference was statistically significant (t =7.29,P ＜ 0.01 ; t =33.24,P ＜ 0.01) ; and neuronal activity was strongest.Lactate dehydrogenase activity was significantly decreased after hypoxia 72 h,and also a statistically significant difference was found between the groups at each point.Conclusions The EPO and EPO mRNA expression are increased after hypoxia in neuron cells,and may enhance the activity of neurons.Our study suggests that EPO might be involved in the development process of neuronal hypoxia and play an important role in neuronal hypoxia process.

Objective To investigate the expression of hypoxia-inducible factor 1α (HIF-1α)gene in rat cerebral cortex neurons under hypoxia conditions,and provide more experimental basis for clinical treatment of hypoxic brain disease.Methods The rat cerebral cortical neurons in primary culture and hypoxia model were prepared and identified by immunocytochemistry analysis.The expression of HIF-1α in normal and hypoxic neurons was detected at 4 time points (12 h,24 h,48 h and 72 h) by immunohistochemical analysis.Results The expression of HIF-1α positive cells in normal control (NC) group was less at each time point,and it showed no statistically significant within groups.The weak expression of HIF-1α was found at 12 h in hypoxic group,and the expression augmented along with the time extended,it increased obviously at 24 h,reached the peak at 48 h(IOD＝0.27±0.02,F＝35.703,t＝11.795,P＜0.01),and declined until 72 h,it showed statistical significance between two groups at each time point.Conclusions The expression of HIF-1α is increased after hypoxia.HIF-1α has a protective effect on neurons after hypoxia.

The frequency domain of single channel currents is analyzed by the power distribution function (PDF) constructed by the discrete wavelet transform (DWT) and power spectral density (PSD). The result shows that the power distribution function based on DWT is an effective frequency-domain analysis method for single channel currents.
Fourier Analysis ; Ganglia, Spinal ; physiology ; Humans ; Ion Channels ; physiology ; Mathematics ; Membrane Potentials ; Models, Biological ; Signal Processing, Computer-Assisted

OBJECTIVE To estimate the distribution of pathogens of nosocomial infection in intensive care units,the sites where infections often taken place and the related antibiotic-resistance of bacteria.METHODS Various samples of bacteria from intensive care units have been examined by applying half-automatic analyzer ATB Expression,with bacteria inspecting cards and drug-sensitivity cards,all from Bio-Merieux,France.RESULTS Totally 153 strains of nosocomial pathogens have been found,where Acinebacter baumannii,fungi and Pseudomonas aeruginosa ranked top three,with rates of 32.0%,16.3%,and 15.0%,respectively.About 45.8% of pathogens were found in samples from the lower respiratory tract.Approximately 4.3% of A.baumannii showed drug-resistance to imipenem.However,more than 50.0% of A.baumannii presented its strong drug-resistance to rest of eight regular antibiotics.CONCLUSIONS A.baumannii is a dominating pathogen to cause nosocomial infection in intensive care units,and has shown strong multi-drug-resistance,which should incur high clinical attention.It comes to be crucial to highlight sanitization and sterilization operation,applying proper antibiotics in order to reduce appearance of bacteria with drug-resistance.