1.Preliminary Study on the Relationship between Cancer Testis Antigen ACRBP and Immune Infiltration of Liver Cancer
Zhaoyue ZENG ; Zhenkai ZHAO ; Jianan JIANG
Journal of Medical Research 2025;54(1):79-86
Objective To explore the expression of cancer-testis antigen ACRBP in liver cancer and its correlation with immune infiltration.Methods Firstly,UALCAN and HPA databases were used to analyze the expression of ACRBP mRNA and protein both in liver cancer and normal liver tissues.The correlation between the expression of ACRBP in liver cancer and immune cell infiltration,and immune regulation genes were analyzed based on TCGA data,TIMER database,CIBERSOFT tool and TISIDB database.Then,human peripheral blood mononuclear cells(PBMC)were isolated and co-cultured with liver cancer cells with down-regulated ACRBP expres-sion.The secretion of immune molecules was measured by ELISA,and the apoptosis of PBMC was detected by flow cytometry.Results Bioinformatics analysis revealed that the expression of ACRBP mRNA and protein in liver cancer tissues was significantly higher than those in normal liver tissues.The expression of ACRBP mRNA was positively correlated with Treg cell infiltration,immunosuppressive mole-cules,chemokines and other immunoregulatory genes.Compared with normal liver tissues,liver cancer tissues with high expression of ACRBP was significantly reduced in the infiltration of Plasma cells,γδT cells,NK cells and M2 macrophages.In vitro,by down-regu-lateing the expression of ACRBP in liver cancer cells,the secretion of TNF-α,IFN-γ and IL-2 was increased,and the apoptosis of PBMC was also increased.Conclusion The expression of ACRBP in live cancer is associated with the infiltration of immune cells.ACRBP participates in the regulation of immune cells and influences the tumor microenvironment,laying the foundation for the future de-velopment of immunotherapy targeting ACRBP.
2.Preliminary Study on the Relationship between Cancer Testis Antigen ACRBP and Immune Infiltration of Liver Cancer
Zhaoyue ZENG ; Zhenkai ZHAO ; Jianan JIANG
Journal of Medical Research 2025;54(1):79-86
Objective To explore the expression of cancer-testis antigen ACRBP in liver cancer and its correlation with immune infiltration.Methods Firstly,UALCAN and HPA databases were used to analyze the expression of ACRBP mRNA and protein both in liver cancer and normal liver tissues.The correlation between the expression of ACRBP in liver cancer and immune cell infiltration,and immune regulation genes were analyzed based on TCGA data,TIMER database,CIBERSOFT tool and TISIDB database.Then,human peripheral blood mononuclear cells(PBMC)were isolated and co-cultured with liver cancer cells with down-regulated ACRBP expres-sion.The secretion of immune molecules was measured by ELISA,and the apoptosis of PBMC was detected by flow cytometry.Results Bioinformatics analysis revealed that the expression of ACRBP mRNA and protein in liver cancer tissues was significantly higher than those in normal liver tissues.The expression of ACRBP mRNA was positively correlated with Treg cell infiltration,immunosuppressive mole-cules,chemokines and other immunoregulatory genes.Compared with normal liver tissues,liver cancer tissues with high expression of ACRBP was significantly reduced in the infiltration of Plasma cells,γδT cells,NK cells and M2 macrophages.In vitro,by down-regu-lateing the expression of ACRBP in liver cancer cells,the secretion of TNF-α,IFN-γ and IL-2 was increased,and the apoptosis of PBMC was also increased.Conclusion The expression of ACRBP in live cancer is associated with the infiltration of immune cells.ACRBP participates in the regulation of immune cells and influences the tumor microenvironment,laying the foundation for the future de-velopment of immunotherapy targeting ACRBP.
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