Objective:To compare the effects of two embryo culture reagents on embryonic developmental time dynamics, embryonic development potential and clinical pregnancy outcomes.Methods:The data of patients undergoing in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) were retrospectively analyzed at the Center for Reproductive Medicine of the First Affiliated Hospital of Zhengzhou University from September 2016 to May 2018. According to the different embryo culture reagents, they were divided into Vitrolife group and Cook group, comparing the embryonic time dynamics parameters, embryo development potential and clinical pregnancy outcomes of 470 patients who met the criteria. Results:There were significant differences in the kinetic parameters of embryo development between the two different embryo culture reagents. Compared with Cook group, Vitrolife group had shorter time in pronuclei appeare and disappear, and time of development to 3-cell and 8-cell embryos [tPNa: (7.12±2.71) h vs. (7.40±2.61) h, tPNf: (23.83±4.33) h vs. (24.21±4.74) h, t3: (35.75±6.03) h vs. (36.64±6.16) h, t4: (38.30±6.25) h vs. (38.92±6.06) h, t5: (47.59±7.85) h vs. (49.01±7.86) h, t6: (50.77±7.17) h vs. (52.12±6.99) h, t7: (53.05±6.31) h vs. (54.33±6.37) h, t8: (55.35±6.89) h vs. (56.31±6.41) h, all P<0.05]. There was no significant difference in cell cycle s2 and had a significant difference in cc2 [9.71±4.60) h vs.(10.33±4.28) h, P<0.001], synchronized in cell cycle in the sexual comparison. Cook group had a longer cell cycle, cell development time interval t5-t4 [(10.60±5.65) h vs. (11.20±5.90) h], t8-t4 [(18.45±5.76) h vs. (19.28±5.18) h] with statistically significant differences, all P<0.05, but there was no significant difference at t2 and t4-t2. Comparing the embryo development potential of the two embryo culture reagents, embryo utilization rate (59.9% vs. 63.9%, P=0.017) and day 3 (D3) high-quality embryo rate (65.4% vs. 69.5%, P=0.011) in Cook group were higher than those in Vitrolife group. There were no significant differences in implantation rate, embryo implantation rate, blastocyst implantation rate, fertilization rate and cleavage rate ( P>0.05). The clinical pregnancy rate, the continuous pregnancy rate (OPR), the abortion rate, the delivery rate, the live birth rate, the fetal malformation rate, the male to female ratio, the embryo pregnancy rate, and the blastocyst pregnancy rate were not statistically different ( P>0.05). Conclusion:According to the actual situation of the center, each center should find out the embryo time kinetic parameters suitable for the embryo culture reagent of the center, and establish the selection standard of the best embryo culture reagent, so as to improve the clinical pregnancy rate and live birth rate of the patients in the center.

Objective:To compare the effects of two embryo culture reagents on embryonic developmental time dynamics, embryonic development potential and clinical pregnancy outcomes.Methods:The data of patients undergoing in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) were retrospectively analyzed at the Center for Reproductive Medicine of the First Affiliated Hospital of Zhengzhou University from September 2016 to May 2018. According to the different embryo culture reagents, they were divided into Vitrolife group and Cook group, comparing the embryonic time dynamics parameters, embryo development potential and clinical pregnancy outcomes of 470 patients who met the criteria. Results:There were significant differences in the kinetic parameters of embryo development between the two different embryo culture reagents. Compared with Cook group, Vitrolife group had shorter time in pronuclei appeare and disappear, and time of development to 3-cell and 8-cell embryos [tPNa: (7.12±2.71) h vs. (7.40±2.61) h, tPNf: (23.83±4.33) h vs. (24.21±4.74) h, t3: (35.75±6.03) h vs. (36.64±6.16) h, t4: (38.30±6.25) h vs. (38.92±6.06) h, t5: (47.59±7.85) h vs. (49.01±7.86) h, t6: (50.77±7.17) h vs. (52.12±6.99) h, t7: (53.05±6.31) h vs. (54.33±6.37) h, t8: (55.35±6.89) h vs. (56.31±6.41) h, all P<0.05]. There was no significant difference in cell cycle s2 and had a significant difference in cc2 [9.71±4.60) h vs.(10.33±4.28) h, P<0.001], synchronized in cell cycle in the sexual comparison. Cook group had a longer cell cycle, cell development time interval t5-t4 [(10.60±5.65) h vs. (11.20±5.90) h], t8-t4 [(18.45±5.76) h vs. (19.28±5.18) h] with statistically significant differences, all P<0.05, but there was no significant difference at t2 and t4-t2. Comparing the embryo development potential of the two embryo culture reagents, embryo utilization rate (59.9% vs. 63.9%, P=0.017) and day 3 (D3) high-quality embryo rate (65.4% vs. 69.5%, P=0.011) in Cook group were higher than those in Vitrolife group. There were no significant differences in implantation rate, embryo implantation rate, blastocyst implantation rate, fertilization rate and cleavage rate ( P>0.05). The clinical pregnancy rate, the continuous pregnancy rate (OPR), the abortion rate, the delivery rate, the live birth rate, the fetal malformation rate, the male to female ratio, the embryo pregnancy rate, and the blastocyst pregnancy rate were not statistically different ( P>0.05). Conclusion:According to the actual situation of the center, each center should find out the embryo time kinetic parameters suitable for the embryo culture reagent of the center, and establish the selection standard of the best embryo culture reagent, so as to improve the clinical pregnancy rate and live birth rate of the patients in the center.

OBJECTIVETo establish a new extrahepatic growing hepatocellular carcinoma cell line.

METHODSA specimen from extrahepatic growing hepatocellular carcinoma was cultured in vitro. Cancer cells were studied morphologically and subjected to karyotype analysis, DNA analysis, and tumor formation evaluation.

RESULTSMorphological observation and functional analysis showed that their features were similar to those of HCC. Chromosomes with a variation of 76 approximately 104 were able to secret AFP in vitro and to form bile canaliculi with microvilli.

CONCLUSIONEGHC-9901 cell line has characteristics of the extrahepatic growing hepatocellular carcinoma.

Adult ; Animals ; Carcinoma, Hepatocellular ; genetics ; pathology ; Chromosome Aberrations ; Humans ; Liver Neoplasms ; genetics ; pathology ; Male ; Mice ; Mice, Nude ; Tumor Cells, Cultured ; alpha-Fetoproteins ; analysis

To establish a new extrahepatic growing hepatocellular carclnoma cell line. Methods: A speciment that was pathologically identified extrahepatic growing hepatocellular carcinoma were cultured in vitro. The morphology,karyotype analysis, DNA analysis, the tumor formation of heterotransplantation were observed. Results: Morphological observation and functional analysis showed that it had the comnnon features of HOC.It was found to be able to secret AFP in vitro. Conclusion: EGHC-9901 has characteristic of the extrahepatic growing hepatocellular carcinoma cell line.