Objective To explore the effect of hypoxia on the proliferation of human umbilical vein endothelia cell (HUVEC) and its underlying mechanism.Methods HUVEC were incubated at different oxygen concentrations (1％,5％,21％) and at 5％ CO2 at a temperature of 37 ℃ for 24 hours.Proliferation rate was detected by WST-1.The target gene of miR-99a was predicted by bioinformatics and verified by double luciferase reporter assay.MiR-99a mimics,miR-99a siRNA,mTOR siRNA,or mTOR cDNA were transfected in HUVEC by lipofectamine 2000,and then incubated in hypoxia.Mir-99a and mTOR were respectively detected by qRT-PCR and RT-PCR or Western blot.Results The proliferation of HUVEC was obviously decreased under hypoxia (1％ O2) (33.3％ ± 6.5％),as compared with normoxia(21％ 02) (P =0.004).Hypoxia could up-regulate the expression level of miR-99a (1.94 ± 0.24,P =0.001),which further inhibited the proliferation of HUVEC.Bioinformatics and double luciferase reporter assay indicated that mTOR was the target gene of miR-99a.Under hypoxia,the gene and protein level of mTOR were down-regulated by miR-99a.Further research demonstrated that the proliferation of HUVEC was inhibited under hypoxia by miR-99a-mTOR pathway.Conclusions Hypobaric treatment could significantly inhibit the proliferation of HUVEC,which might be associated with miR-99a-mTOR pathway.

Objective To explore the effect of hypoxia on the proliferation of human umbilical vein endothelia cell (HUVEC) and its underlying mechanism.Methods HUVEC were incubated at different oxygen concentrations (1％,5％,21％) and at 5％ CO2 at a temperature of 37 ℃ for 24 hours.Proliferation rate was detected by WST-1.The target gene of miR-99a was predicted by bioinformatics and verified by double luciferase reporter assay.MiR-99a mimics,miR-99a siRNA,mTOR siRNA,or mTOR cDNA were transfected in HUVEC by lipofectamine 2000,and then incubated in hypoxia.Mir-99a and mTOR were respectively detected by qRT-PCR and RT-PCR or Western blot.Results The proliferation of HUVEC was obviously decreased under hypoxia (1％ O2) (33.3％ ± 6.5％),as compared with normoxia(21％ 02) (P =0.004).Hypoxia could up-regulate the expression level of miR-99a (1.94 ± 0.24,P =0.001),which further inhibited the proliferation of HUVEC.Bioinformatics and double luciferase reporter assay indicated that mTOR was the target gene of miR-99a.Under hypoxia,the gene and protein level of mTOR were down-regulated by miR-99a.Further research demonstrated that the proliferation of HUVEC was inhibited under hypoxia by miR-99a-mTOR pathway.Conclusions Hypobaric treatment could significantly inhibit the proliferation of HUVEC,which might be associated with miR-99a-mTOR pathway.

Objective To analyze the prevention and control condition of infectious diseases of national health cities of prefecture and county level and non-national health cities in Shandong province according to the requirement to create national health cities.Methods To select 15 cities in Shandong province as the research object,including the 5 prefecture-level national health cities,5 county-level national health cities and 5 non-national health city closer to the national health situation.The survey status of infectious diseases,infectious morbidity and children′s vaccination status were analyzed,respectively.Results From 2005 to 2008,registration reporting rate,timeliness rate,accuracy rate and integrity rate of legal infectious diseases increased to 99％ or more.Direct reporting network coverage rate of 1infectious diseases and public health emergencies reached 100％.No type A and B infectious diseases outbreak occurred.The incidences of hepatitis B,dysentery and tuberculosis of national health city were all lower than the rates of non-state health cities and the national average level.The care rate of mobile children living more than 3 months and the immunization rate reached 100％ at county-level cities.Immunization rates of child 4 vaccines and hepatitis B vaccination were more than 99％ annually in the year 2007 and 2008.Conclusions The status of prevention and treatment of infectious diseases is effective.The national health city had a more powerful emergency response ability in Shandong province.