Objective To assess the clinical efficacy of sodium hyaluronate （0.3%） eye drops combined with herbal self-heating steam eye mask in the treatment of dry eye disease. Methods A prospective randomized controlled clinical trial was performed on 60 patients diagnosed with dry eye at the ophthalmic clinic of a Grade A, Class Ⅲ hospital in Shanghai from June 2023 to September 2024. Specifically, patients were randomly divided into control group and study group. Patients in the control group were treated with sodium hyaluronate （0.3%） eye drops for six weeks; while in the study group, patients received the eye drops combined with the herbal self-heating steam eye mask mainly containing powders of Flos Buddlejae. Subsequently, comparisons and analysis were performed before and after treatment between the two groups in the clinical symptom questionnaire score traditional Chinese medicine （TCM syndrome score），the Chinese dry eye questionnaire score and determination of tear film fluorscein breakup time （FBUT）, and curative effect. Results The quality control standard of the herbal powder in the self-heating steam eye mask was established through TLC and HPLC, and good heating behavior of the herbal self-heating steam eye mask was ascertained heating temperature （43±5）℃; heating duration （≥20 min）, meeting requirements of the product quality control. After treatment for 6 weeks, FBUT was increased, while TCM syndrome score and the Chinese dry eye questionnaire score were both decreased in the study group （P<0.001）. Besides, compared with the control group, TCM syndrome score and the Chinese dry eye questionnaire score were much lower, while the FBUT were higher in the study group （P<0.001）. Moreover, the overall response rate in the study group （81.7%） was much better than that in the control group （25.9%）. Conclusion The combination of sodium hyaluronate （0.3%） eye drops with herbal self-heating steam eye mask could be applied to the clinical treatment of dry eye disease due to its good clinical effects on relieving dry eye symptoms.

Objective To establish a HPLC method for determination of berberine hydrochloride in Dongbai Tonglin Heji.Methods The separation column of Kromasil C18(150 mm×4.6 mm,5 μm) was used.The mobile phase was acetonitrile and 0.05 mol/L NaH2PO4 solution(pH was adjusted to about 3 with phosphoric acid).The flow rate was 1.0 ml/min with the column at room temperature and the detection wavelengths at 345 nm for berberine hydrochloride.Results The linear range of berberine hydrochloride was 1.00~50.00 μg/ml(r=0.999 6).The average recoveries of berberine hydrochloride were 104.70%(RSD 1.60%, n=5).Conclusion This method is accurate, sensitive, selective and reproducible.It provides an alternative method to improve the quality control of Dongbai Tonglin Heji.

Objective To investigate the off-label use of antibacterials in out-patient department and emergency depart-mentand give reference for management .Methods About 10% of antibacterials prescriptions in out-patient department and emergency department were randomly extracted .Off-label use was analyzed by assessing off-label use percentage ,reviewing off-label types and evaluating the rationality .Results 23 600 antibacterial prescriptions ,including 43 antibacterials in 9 catego-rieswere analyzed ,and the off-label use percentage of 22 .00% .Cephalosporins contributed most in the amount of off-label use prescriptions ,while nitroimidazoleseries got the highest off-label percentage of 73 .45% .Improper frequency and dosing were the most obvious phenomena ,with the ratio of 55.64% and 52 .60% ,respectively .For oral drugs ,most frequent off-label use type of these drugs was improper dosing (72 .46% ) .For injections ,frequent off-label use type of these drugs were manifested improper frequency (82 .18% ) and improper solvent (38 .80% ) .Conclusion Off-label use of antibacterials in hospital was in a critical situation and most of the prescriptions were lack of rational evidence .Standardized administration should be introduced .

In this paper, absorption and pharmacokinetic study of Radix Rehmanniae was studied by liquid chromatography coupled with mass spectrometry method after oral administration to rats. By comparing the chromatograms of ultraviolet, full scan, extracted ion and selective reaction monitoring (SRM) of standard solution, Radix Rehmanniae, blank plasma and rat plasma post drug administration, catalpol and ajugol were found to be the main compounds absorbed from Radix Rehmanniae. Plasma concentrations of aucubin, dihydrocatalpol, rehmannioside A (or rehmannioside B/ melittoside) and rehmannioside D were very low. Quantitative method for catalpol and aucubin and semi-quantitative method for other compounds in rat plasma were established. The pharmacokinetic study of those absorbed components was conducted after oral administration of 6 g x kg(-1) Radix Rehmanniae water extract to rats. Cmax, t(1/2) and AUC(0-infinity) of catalpol and ajugol were (2349.05 +/- 1438.34) and (104.25 +/- 82.05) ng x mL(-1), (0.86 +/- 0.32) and (0.96 +/- 0.37) h, (4407.58 +/- 2734.89) and (226.66 +/- 188.38) ng x h x mL(-1), respectively. tmax was at 1.00 h for catalpol and ajugol. Both catalpol and ajugol were absorbed and excreted rapidly.