ObjectiveTo reveal the effects of overexpression and knockdown of CDH18 gene on cervical cancer proliferation and migration levels through the Wnt/β-catenin signaling pathway in cervical cancer cells. MethodsA comprehensive analysis of CDH18-interacting molecules was performed via the bioinformatics STRING database （http：//cn.string-db.org）. Molecular docking between Wnt/β-catenin inhibitor XAV939 and β-catenin was performed using the bioinformatics tool AutoDock （http：//autodock.scripps.edu）. The effect of CDH18 on β-catenin subcellular distribution was detected by laser confocal microscopy. EdU assay， wound healing assay and Transwell assay were used to detect the effects of CDH18 gene overexpression， CDH18 gene knockdown and the XAV939 on cell proliferation and migration. Western blot was performed to measure the expression levels of Wnt/β-catenin pathway-related molecules， including β-catenin， transcription factor 4 （TCF4）， and cellular myelocytomatosis oncogene （c-Myc）， following CDH18 gene overexpression， CDH18 gene knockdown， or XAV939 treatment. ResultsIn SiHa and HeLa cells， CDH18 gene overexpression significantly increased the protein expression levels of β-catenin， TCF4 and c-Myc， decreased the protein expression of GSK-3β， and enhanced cell proliferation and migration， with statistically significant differences （P<0.05）. Conversely， CDH18 gene knockdown significantly reduced the protein expression of β-catenin， TCF4 and c-Myc， elevated GSK-3β expression， and suppressed cell proliferation and migration （P<0.05）.Treatment with XAV939 in SiHa and HeLa cells downregulated β-catenin， TCF4 and c-Myc， upregulated GSK-3β， and inhibited cell proliferation and migration， with significant differences （P<0.05）. ConclusionCDH18 promotes the proliferation and migration of cervical cancer cells via the Wnt/β-catenin signaling pathway.

Objective To discuss the safety and feasibility of extending the main operating port in the right lower abdomen for specimen collection while performing preventive ileostomy during laparoscopic anterior resection of rectal cancer.Methods A retrospective analysis of clinical data of 130 cases of laparoscopic anterior resection of rectal cancer combined with preventive ileostomy performed by the same surgeon from April 2021 to April 2024 was carried out.For patients with good mobility of the sigmoid colon and no significant edema of the mesentery,the main operating port in the right lower abdomen was extended to obtain specimens and perform ileostomy ( observation group,n=56 ).For those with insufficient mobility,short colon,and mesenteric edema,specimens were obtained through an auxiliary incision around the left of the umbilicus,and ileostomy was performed at the main operating port in the right lower abdomen (control group,n=74).The intraoperative indicators,postoperative indicators,and stoma-related complications of the two groups were compared.Results Both groups successfully completed the surgery.Compared to the control group,the observation group had a shorter surgery time[(133.6±7.1) min vs.(136.2±7.3) min,t=-1.972,P=0.005],lower pain scores at 24 h and 48 h postoperatively[(2.6±1.1) points vs.(2.9±1.3) points,t=-1.711,P=0.017;(1.5±0.6) points vs.(1.6±0.6) points,t=-1.437,P=0.042],and a shorter hospital stay postoperatively[(6.9±1.2) d vs.(7.2±1.3) d,t=-1.411,P=0.045].There were no significant differences in intraoperative blood loss,time to colostomy gas passage,time to start a liquid diet,and incidence of complications between the two groups (P>0.05).Conclusion For laparoscopic anterior resection combined with preventive ileostomy for low-to-mid rectal cancer,extending the main operating port in the right lower abdomen to obtain specimens and performing ileostomy is safe and feasible when there are good mobilization of the sigmoid colon and no significant edema of the mesentery,aligning with the principles of enhanced recovery after surgery.

Objective To discuss the safety and feasibility of extending the main operating port in the right lower abdomen for specimen collection while performing preventive ileostomy during laparoscopic anterior resection of rectal cancer.Methods A retrospective analysis of clinical data of 130 cases of laparoscopic anterior resection of rectal cancer combined with preventive ileostomy performed by the same surgeon from April 2021 to April 2024 was carried out.For patients with good mobility of the sigmoid colon and no significant edema of the mesentery,the main operating port in the right lower abdomen was extended to obtain specimens and perform ileostomy ( observation group,n=56 ).For those with insufficient mobility,short colon,and mesenteric edema,specimens were obtained through an auxiliary incision around the left of the umbilicus,and ileostomy was performed at the main operating port in the right lower abdomen (control group,n=74).The intraoperative indicators,postoperative indicators,and stoma-related complications of the two groups were compared.Results Both groups successfully completed the surgery.Compared to the control group,the observation group had a shorter surgery time[(133.6±7.1) min vs.(136.2±7.3) min,t=-1.972,P=0.005],lower pain scores at 24 h and 48 h postoperatively[(2.6±1.1) points vs.(2.9±1.3) points,t=-1.711,P=0.017;(1.5±0.6) points vs.(1.6±0.6) points,t=-1.437,P=0.042],and a shorter hospital stay postoperatively[(6.9±1.2) d vs.(7.2±1.3) d,t=-1.411,P=0.045].There were no significant differences in intraoperative blood loss,time to colostomy gas passage,time to start a liquid diet,and incidence of complications between the two groups (P>0.05).Conclusion For laparoscopic anterior resection combined with preventive ileostomy for low-to-mid rectal cancer,extending the main operating port in the right lower abdomen to obtain specimens and performing ileostomy is safe and feasible when there are good mobilization of the sigmoid colon and no significant edema of the mesentery,aligning with the principles of enhanced recovery after surgery.