The 5th edition of the World Health Organization central nervous system(5th WHO CNS)incorporated molecular alterations as essential diagnostic criteria,placing increased demands on pathologists.Understanding the e-volving relationship between molecular changes and traditional histological features is challenging,and integrating both to establish a definitive pathological diagnosis requires substantial experience and expertise.This article aimed to pro-vide a practical reference for domestic pathologists by interpreting the standardized dataset for central nervous system tumors released by the International Collaboration on Cancer Reporting(ICCR)in 2024,covering histological,molecu-lar,and integrated diagnostic components.

Purpose To investigate the correlation between two autophagy-related proteins,JMY and WHAMM,and autophagic dysfunction and prognosis in gliomas.Methods A total of 113 cases of diffuse gliomas and 14 control brain tissues were collected.The expression of JMY,WHAMM,and autophagy-related markers were evaluated using the EnVision two-step method of immunohistochemistry.Correlations between the expression of JMY and WHAMM and clinicopathological features,prognosis,and autophagy markers were analyzed.Results The high expression rates of JMY and WHAMM in control brain tissue were significantly higher than those in diffuse gliomas.The expressions of JMY and WHAMM were associated with pathological grade and age.Compared with low-grade gliomas(LGG),high-grade gliomas(HGG)showed significantly lower high-expression rates of JMY and WHAMM.Patients with low expres-sion of JMY and WHAMM had shorter overall survival than those with high expression.Cox regression analysis indica-ted that the high pathological grade and low expression of JMY were independent prognostic risk factors.The expression profiles of autophagy markers suggested reduced autophagic activity in HGGs,and JMY expression was positively corre-lated with autophagy markers.Conclusion The high expression rates of JMY and WHAMM are significantly lower in HGGs compared to LGGs.JMY and WHAMM may serve as potential molecular markers for the prognostic evaluation of diffuse gliomas.

The 5th edition of the World Health Organization central nervous system(5th WHO CNS)incorporated molecular alterations as essential diagnostic criteria,placing increased demands on pathologists.Understanding the e-volving relationship between molecular changes and traditional histological features is challenging,and integrating both to establish a definitive pathological diagnosis requires substantial experience and expertise.This article aimed to pro-vide a practical reference for domestic pathologists by interpreting the standardized dataset for central nervous system tumors released by the International Collaboration on Cancer Reporting(ICCR)in 2024,covering histological,molecu-lar,and integrated diagnostic components.

Purpose To investigate the correlation between two autophagy-related proteins,JMY and WHAMM,and autophagic dysfunction and prognosis in gliomas.Methods A total of 113 cases of diffuse gliomas and 14 control brain tissues were collected.The expression of JMY,WHAMM,and autophagy-related markers were evaluated using the EnVision two-step method of immunohistochemistry.Correlations between the expression of JMY and WHAMM and clinicopathological features,prognosis,and autophagy markers were analyzed.Results The high expression rates of JMY and WHAMM in control brain tissue were significantly higher than those in diffuse gliomas.The expressions of JMY and WHAMM were associated with pathological grade and age.Compared with low-grade gliomas(LGG),high-grade gliomas(HGG)showed significantly lower high-expression rates of JMY and WHAMM.Patients with low expres-sion of JMY and WHAMM had shorter overall survival than those with high expression.Cox regression analysis indica-ted that the high pathological grade and low expression of JMY were independent prognostic risk factors.The expression profiles of autophagy markers suggested reduced autophagic activity in HGGs,and JMY expression was positively corre-lated with autophagy markers.Conclusion The high expression rates of JMY and WHAMM are significantly lower in HGGs compared to LGGs.JMY and WHAMM may serve as potential molecular markers for the prognostic evaluation of diffuse gliomas.