Objective To investigate the current status of construction and monitoring of healthcare-associated in-fection(HAI)system in maternal and child health care institutions in Guangxi Zhuang Autonomous Region,and provide reference for improving the quality of HAI management.Methods After discussed by members of expert group of HAI Prevention and Control Professional Committee of Guangxi Maternal and Child Health Care Associa-tion,the"Questionnaire on the construction and monitoring of HAI system in maternal and child health care institu-tions in Guangxi Zhuang Autonomous Region"was designed.The questionnaires were distributed to all maternal and child health care institutions in the region via"Questionnaire Star",and a WeChat group was established to ad-dress any questions in questionnaires.Results A total of 104 maternal and child health care institutions were in-cluded in the study,including 6 primary,74 secondary,and 24 tertiary level institutions.All maternal and child health care institutions have established HAI management departments,95.19％(n=99)of institutions held HAI management committee meeting at least once every six months.Among 201 HAI management professionals,most were female(90.55％),with nursing specialty(72.64％),with a bachelor's degree(66.17％),and aged 31-50 years old(73.13％).Only 30.77％of the maternal and child health care institutions had HAI monitoring system.Seventy institutions(67.30％)have carried out the special action of improving the pathogen detection rate before antimicrobial therapy for hospitalized patients,out of which 55(78.57％)were led by the HAI management depart-ment.Ninety-seven(92.27％)maternal and child health care institutions conducted surgery-related monitoring,with 50.96％of institutions relying on manual monitoring;only 30.77％of institutions have carried out the special action of"Strengthening perioperative infection prevention and control to ensure surgical quality and safety".Conclusion HAI management system in maternal and child health care institutions in Guangxi has been basically es-tablished and can carry out monitoring work,but personnel compostion is limited and talent reserve is insufficient.The information construction of HAI monitoring is underdeveloped,with a lagged implement of special action.Ad-ministrative support is needed to enhance infection prevention and control at the grassroots level.All levels of ma-ternal and child health care institutions should communicate and exchange work experience.Hospitals should in-crease their support for the construction of HAI information technology,improve level of HAI monitoring as well as prevention and control capabilities.

Objective To analyze the causes and intervention measure efficacy of an outbreak of neonatal Pseudo-monas aeruginosa(P.aeruginosa)infection in a medical institution.Methods The epidemiological investigation was conducted on 5 neonates who had P.aeruginosa infection in the neonatal intensive care unit(NICU)of this medical institution from May 26 to June 10,2024.Specimens were taken from potentially contaminated environ-ments to infer the cause of P.aeruginosa transmission in the NICU,infection control measures were also taken,and the efficacy of the control measures was continuously monitored.Results Among the 5 infected neonates,3 had lower respiratory tract infection and 2 had lower respiratory tract combined with bloodstream infection.Seven strains of P.aeruginosa were detected with a consistent antimicrobial resistance spectrum.Infected neonates were in the same ward and had spatial and temporal clustering.Through environmental hygiene monitoring,P.aerugi-nosa with consistent antimicrobial resistance spectrum of neonatal infection was isolated from the NICU handwashing sink and water-related environment(suction connection pipe,bedside isolation gowns).Infection was controlled af-ter improving sink disinfection methods and enhancing contact isolation measures,and no further P.aeruginosa was detected in the environment.Conclusion The outbreak of neonatal HAI in the NICU is inferred to be caused by contamination of ward handwashing sink by P.aeruginosa,then further contaminated the suction connection tube,thereby leading to the transmission of neonatal infection through suction procedures.Healthcare-associated infection surveillance and control professionals need to fully understand the characteristics of outbreak pathogens and take tar-geted measures to curb the transmission of neonatal infection.

Objective To analyze the causes and intervention measure efficacy of an outbreak of neonatal Pseudo-monas aeruginosa(P.aeruginosa)infection in a medical institution.Methods The epidemiological investigation was conducted on 5 neonates who had P.aeruginosa infection in the neonatal intensive care unit(NICU)of this medical institution from May 26 to June 10,2024.Specimens were taken from potentially contaminated environ-ments to infer the cause of P.aeruginosa transmission in the NICU,infection control measures were also taken,and the efficacy of the control measures was continuously monitored.Results Among the 5 infected neonates,3 had lower respiratory tract infection and 2 had lower respiratory tract combined with bloodstream infection.Seven strains of P.aeruginosa were detected with a consistent antimicrobial resistance spectrum.Infected neonates were in the same ward and had spatial and temporal clustering.Through environmental hygiene monitoring,P.aerugi-nosa with consistent antimicrobial resistance spectrum of neonatal infection was isolated from the NICU handwashing sink and water-related environment(suction connection pipe,bedside isolation gowns).Infection was controlled af-ter improving sink disinfection methods and enhancing contact isolation measures,and no further P.aeruginosa was detected in the environment.Conclusion The outbreak of neonatal HAI in the NICU is inferred to be caused by contamination of ward handwashing sink by P.aeruginosa,then further contaminated the suction connection tube,thereby leading to the transmission of neonatal infection through suction procedures.Healthcare-associated infection surveillance and control professionals need to fully understand the characteristics of outbreak pathogens and take tar-geted measures to curb the transmission of neonatal infection.

Objective To investigate the current status of construction and monitoring of healthcare-associated in-fection(HAI)system in maternal and child health care institutions in Guangxi Zhuang Autonomous Region,and provide reference for improving the quality of HAI management.Methods After discussed by members of expert group of HAI Prevention and Control Professional Committee of Guangxi Maternal and Child Health Care Associa-tion,the"Questionnaire on the construction and monitoring of HAI system in maternal and child health care institu-tions in Guangxi Zhuang Autonomous Region"was designed.The questionnaires were distributed to all maternal and child health care institutions in the region via"Questionnaire Star",and a WeChat group was established to ad-dress any questions in questionnaires.Results A total of 104 maternal and child health care institutions were in-cluded in the study,including 6 primary,74 secondary,and 24 tertiary level institutions.All maternal and child health care institutions have established HAI management departments,95.19％(n=99)of institutions held HAI management committee meeting at least once every six months.Among 201 HAI management professionals,most were female(90.55％),with nursing specialty(72.64％),with a bachelor's degree(66.17％),and aged 31-50 years old(73.13％).Only 30.77％of the maternal and child health care institutions had HAI monitoring system.Seventy institutions(67.30％)have carried out the special action of improving the pathogen detection rate before antimicrobial therapy for hospitalized patients,out of which 55(78.57％)were led by the HAI management depart-ment.Ninety-seven(92.27％)maternal and child health care institutions conducted surgery-related monitoring,with 50.96％of institutions relying on manual monitoring;only 30.77％of institutions have carried out the special action of"Strengthening perioperative infection prevention and control to ensure surgical quality and safety".Conclusion HAI management system in maternal and child health care institutions in Guangxi has been basically es-tablished and can carry out monitoring work,but personnel compostion is limited and talent reserve is insufficient.The information construction of HAI monitoring is underdeveloped,with a lagged implement of special action.Ad-ministrative support is needed to enhance infection prevention and control at the grassroots level.All levels of ma-ternal and child health care institutions should communicate and exchange work experience.Hospitals should in-crease their support for the construction of HAI information technology,improve level of HAI monitoring as well as prevention and control capabilities.

Objective To investigate the clinical effect of different magnetic stimulation pelvic floor modes in the treatment of perimenopausal myofascial pelvic pain syndrome(MPSS).Methods A total of 60 perimenopausal women who were clinically diagnosed with MPSS in the hospital from May 2022 to May 2023 were selected as the research objects.They were divided into groups A,B and C by random number table method,with 20 cases in each group.All patients in the three groups were treated with pelvic floor myofascial manual release.Group A was given pelvic floor magnetic stimulation(alternating 10 Hz and 50 Hz),group B was given sacral nerve root magnetic stimulation(50 Hz),and group C was given pelvic floor magnetic stimu-lation combined with sacral nerve root magnetic stimulation at the same time.The three groups were treated twice a week for eight weeks.Visual analogue scale(VAS)was used to evaluate the degree of pelvic floor myofascial tenderness before and after treatment,and Glazer pelvic floor surface electromyography was used to evaluate pelvic floor muscle function.Results Compared with before treatment,the VAS scores of subjec-tive pain and pelvic floor myofascial tenderness in the three groups were decreased after treatment,and the differences were statistically significant(P<0.05).Compared with group A and group B,the VAS score of subjective pain and the VAS score of pelvic floor myofascial tenderness in group C were significantly decreased after treatment,and the differences were statistically significant(P<0.05).Compared with before treatment,the average amplitude and coefficient of variation(CV)of pre-rest potential and post-rest potential in the three groups were decreased after treatment,and the differences were statistically significant(P<0.05).Compared with before treatment,only the maximum amplitude of rapid contraction,the average amplitude of 10 s sustained contraction and 60 s sustained contraction and CV in group C were improved,and the differ-ences were statistically significant(P<0.05).Compared with group A and group B,the average amplitude and CV of pre-resting potential and post-resting potential in group C were decreased after treatment,the maxi-mum amplitude of rapid contraction and the average amplitude and CV of 10 s continuous contraction and 60 s persistent contraction were improved,and the differences were statistically significant(P<0.05).Conclusion Dif-ferent magnetic stimulation pelvic floor modes can effectively relieve pain and improve pelvic floor muscle strength in the treatment of perimenopausal MPSS,and the effect of pelvic floor magnetic stimulation com-bined with sacral nerve root magnetic stimulation is the best.

Objective:To investigate the effect of recombinant insulin-like growth factor-1 (IGF-1) on ovarian development in MEX3C knockout mice. Methods:Mice were randomly divided into four groups (6 mice in each group): wild type group, homozygous group, homozygous+IGF-1 treatment group, and treatment control group. The weight and wet ovarian weight of mice in each group was compared before and after IGF-1 injection. The serum levels of estradiol was determined using enzyme linked immunosorbent assay (ELISA). The morphological structure of the ovary was observed by HE staining. The expression of MEX3C, IGF-1 receptor (IGF-1R), and the phosphorylation of AKT (Ser473)(p-AKT) were detected by immunohistochemistry and Western blotting.Results:Before IGF-1 injection, the body weight of homozygote group [(6.33±0.31) g] was significantly lighter than that in wild type group [(15.20±0.35) g, P<0.001]. Serum estradiol level was significantly higher in homozygous+IGF-1 treatment group [(17.245 3±0.073 1) ng/L] than in treatment control group [(8.124 8±0.847 7) ng/L, P=0.001]. HE staining results showed that compared with the treatment control group, the primitive follicles (19.83±2.94) and primary follicles (15.50±2.69) were increased in homozygous+IGF-1 treatment group, while the atretic follicles (7.17±1.42) were decreased (all P<0.05). Western blotting results further confirmed that compared with the treatment control group, IGF-1R and p-AKT protein expressions were significantly decreased in homozygous+IGF-1 treatment group ( P=0.014 1, P=0.002 5). Conclusion:IGF-1 promotes ovarian follicular development in MEX3C knockout mice. The mechanism is related to the up-regulation of IGF-1R and P-AKT protein expression. IGF-1 can increase serum estrogen and promote follicular development.

Objective:To investigate the effect of recombinant insulin-like growth factor-1 (IGF-1) on ovarian development in MEX3C knockout mice. Methods:Mice were randomly divided into four groups (6 mice in each group): wild type group, homozygous group, homozygous+IGF-1 treatment group, and treatment control group. The weight and wet ovarian weight of mice in each group was compared before and after IGF-1 injection. The serum levels of estradiol was determined using enzyme linked immunosorbent assay (ELISA). The morphological structure of the ovary was observed by HE staining. The expression of MEX3C, IGF-1 receptor (IGF-1R), and the phosphorylation of AKT (Ser473)(p-AKT) were detected by immunohistochemistry and Western blotting.Results:Before IGF-1 injection, the body weight of homozygote group [(6.33±0.31) g] was significantly lighter than that in wild type group [(15.20±0.35) g, P<0.001]. Serum estradiol level was significantly higher in homozygous+IGF-1 treatment group [(17.245 3±0.073 1) ng/L] than in treatment control group [(8.124 8±0.847 7) ng/L, P=0.001]. HE staining results showed that compared with the treatment control group, the primitive follicles (19.83±2.94) and primary follicles (15.50±2.69) were increased in homozygous+IGF-1 treatment group, while the atretic follicles (7.17±1.42) were decreased (all P<0.05). Western blotting results further confirmed that compared with the treatment control group, IGF-1R and p-AKT protein expressions were significantly decreased in homozygous+IGF-1 treatment group ( P=0.014 1, P=0.002 5). Conclusion:IGF-1 promotes ovarian follicular development in MEX3C knockout mice. The mechanism is related to the up-regulation of IGF-1R and P-AKT protein expression. IGF-1 can increase serum estrogen and promote follicular development.