Objective To explore the medication rules of oral Chinese medicine compound prescriptions in national patents for treating cervical spondylosis through data mining,so as to provide new references for the treatment of cervical spondylosis with traditional Chinese medicine.Methods The data related to patents of oral Chinese medicine compound prescriptions for treating cervical spondylosis were retrieved and screened from the China National Intellectual Property Administration public service website,and a database of Chinese medicine compound prescriptions was established.Relevant software was used to statistically analyze the frequency of drug use,nature,flavor,meridian tropism,and association rules,and cluster analysis was performed on the Chinese medicines with high association intensity.Results A total of 394 compound prescriptions and 221 Chinese medicines were included.Among them,31 Chinese medicines such as Chuanxiong,Danggui,Gegen,Gancao and Weilingxian were high-frequency drugs for treating cervical spondylosis.The nature of medicine was mainly warm,cold and neutral,and the flavor was mainly bitter,pungent and sweet.The meridian tropism was mainly liver,spleen and lung meridians.Cluster analysis of high-frequency drugs yielded 4 clustered prescriptions.Conclusion The treatment of cervical spondylosis with Chinese medicines mainly focuses on promoting blood circulation for removing obstruction in collaterals,activating qi and relieving pain,dispelling pathogenic wind and eliminating dampness,and tonifying and replenishing liver and kidney,with the method of promoting blood circulation for removing blood stasis,and relieving pain running through the whole process.

Objective To explore the medication rules of oral Chinese medicine compound prescriptions in national patents for treating cervical spondylosis through data mining,so as to provide new references for the treatment of cervical spondylosis with traditional Chinese medicine.Methods The data related to patents of oral Chinese medicine compound prescriptions for treating cervical spondylosis were retrieved and screened from the China National Intellectual Property Administration public service website,and a database of Chinese medicine compound prescriptions was established.Relevant software was used to statistically analyze the frequency of drug use,nature,flavor,meridian tropism,and association rules,and cluster analysis was performed on the Chinese medicines with high association intensity.Results A total of 394 compound prescriptions and 221 Chinese medicines were included.Among them,31 Chinese medicines such as Chuanxiong,Danggui,Gegen,Gancao and Weilingxian were high-frequency drugs for treating cervical spondylosis.The nature of medicine was mainly warm,cold and neutral,and the flavor was mainly bitter,pungent and sweet.The meridian tropism was mainly liver,spleen and lung meridians.Cluster analysis of high-frequency drugs yielded 4 clustered prescriptions.Conclusion The treatment of cervical spondylosis with Chinese medicines mainly focuses on promoting blood circulation for removing obstruction in collaterals,activating qi and relieving pain,dispelling pathogenic wind and eliminating dampness,and tonifying and replenishing liver and kidney,with the method of promoting blood circulation for removing blood stasis,and relieving pain running through the whole process.

Objective To analyze the current research status and hotspots in the field of operating room efficiency both domestically and internationally,and provide theoretical and practical guidance for the improvement and evaluation of operating room efficiency.Methods Using bibliometric methods to search for domestic and foreign literature on operating room efficiency in CNKI and Web of Science core collection databases,Citespace software was used for visual analysis of publication volume,core authors,publication institutions,keyword co-occurrence,clustering,and emergence.Results A total of 387 foreign litera-ture on operating room efficiency were retrieved,with the United States ranking first in terms of publication volume.Research in-stitutions mostly collaborate closely,with research hotspots mainly focusing on time management,anesthesia management,cost management,and quality management.Research frontiers include artificial intelligence,quality improvement,etc;A total of 235 domestic literature have been searched,but a close cooperation network has not yet formed.The research hotspots mainly include lean management,process management,nursing management,etc.The research frontiers include information systems,quality and safety,etc.Conclusion China should integrate research capabilities,strengthen institutional cooperation,pay attention to research hotspots and frontiers,and increase the importance of research on operating room efficiency.

Objective To study the relationship between erythromycin sensitivity and drug-resistance gene in Mycoplasma pneu-moniae(Mp).Methods In 46 erythromycin-resistant MP clinical isolates,domain Ⅴ of 23S rRNA was amplified by polymerase chain reaction(PCR),followed by direct automatic sequencing method.The DNA sequences were compared to find molecular mecha-nisms of drug resistance.Results Among the 46 erythromycin-resistant Mp clinical isolates,44(95.65%)harbored an A-to-G tran-sition mutation at position 2063 in the 23S rRNA gene and 2(4.35%)harbored an A-to-G transition mutation at position 2064,but no A-to-C transition mutation at position 2063 and C-to-G/A transition mutation at position 2617 were detected.Conclusion E-rythromycin-resistant of Mp clinical isolates were closely related to A-to-G transition mutation at position 2063/2064 in domain Ⅴof 23S rRNA genes and the most important was the A2063G transition mutation.Rapid and accurate identification of the genetic mutations in domain Ⅴ of 23S rRNA may be help to diagnose the infection of Mycoplasma pneumoniae,provide the drug-resistant information,and instruct the application of antibiotics reasonably and effectively.

Cancer stem cells (CSCs) markers are specific molecules to identify CSCs.Recent findings demonstrate that CSCs markers associated with colorectal cancer mainly include CD133,CD29,CD166,CD44,Nanog,etc.These markers can take park in the initiation and progression of cancers by various molecular mechanisms,which have the potential to be used as therapeutic targets as well as prognostic indicators.

As a member of T-box family,Tbx3 (T-box3) is widely expressed in multiple organs and involves in the development of breast,limb,heart,eye,lung and pancreas during embryonic development stage.Moreover,Tbx3 plays an important role in maintaining the embryonic stem cells.Recent studies show that Tbx3 can work as a transcription factor to participate in the initiation and progression of tumor by epithelialmesenchymal transition,induction tumor stemness and methylation.A better understanding of Tbx3 will provide new insights into genetic diagnosis and targeted treatment of tumor.

Objective To detect the expressions of Y-box-binding protein-1(YB-1)and epithelial-mesenchymal transition(EMT)markers(E-cadherin and N-cadherin)in colorectal cancer(CRC),to analyze the relationship between the expression of YB-1 and clinicopathological parameters,to evaluate the correlations among YB-1,E-cadherin and N-cadherin. Methods The expressions of YB-1,E-cadherin and N-cadherin in 120 primary CRC tumors and corresponding normal tissues were detected by western blot and immunohistochem-istry and the results were analyzed. Results The expressions of YB-1,E-cadherin and N-cadherin in tumors were significantly different from those in corresponding normal tissues(χ2 = 47. 373,P ﹤ 0. 05;χ2 = 83. 145, P ﹤ 0. 05;χ2 = 41. 832,P ﹤ 0. 05). The expression of YB-1 in tumors was associated significantly with tumor differentiation,tumor invasion,lymph node metastasis and distance metastasis(χ2 = 8. 077,P = 0. 008;χ2 =8. 178,P = 0. 006;χ2 = 15. 152,P ﹤ 0. 001;χ2 = 7. 368,P = 0. 011). It was negatively correlated with E-cadherin expression(r = - 0. 238,P = 0. 009),but positively correlated with N-cadherin expression(r =0. 361,P ﹤ 0. 001). Conclusion YB-1 may promote the occurrence and development of CRC by participating in EMT program.

The present paper was aimed to explore the effect of Shuxuetong on the membrane viscoelasticity of erythrocyte taken from the acute phase patients suffering from chronic pulmonary heart disease. The membrane viscoelasticity of erythrocyte was taken from the acute phase patients suffering from chronic pulmonary heart disease. The changes of membrane viscoelasticity of erythrocyte after treated with shuxuetong were detected by micropipette aspiration technique. The results showed that the Shuxuetong of certain concentration could cause the decrease of membrane elastic modulus and viscous coefficients in acute phase patients suffering from chronic pulmonary heart disease. The study offers experimental evidences that the comprehensive treatment of pulmonary heart disease should involve the drug or measure to improve the erythrocyte deformability.
Aged ; Blood Viscosity ; drug effects ; Chronic Disease ; Drugs, Chinese Herbal ; therapeutic use ; Elasticity ; drug effects ; Erythrocyte Deformability ; physiology ; Erythrocyte Membrane ; physiology ; Erythrocytes ; physiology ; Female ; Humans ; Male ; Middle Aged ; Phytotherapy ; Pulmonary Heart Disease ; blood ; drug therapy

The membrane viscoelasticity of erythrocyte taken from both normal subjects and patients with cor pulmonale during acute exacerbation was investigated using a micropipette aspiration technique. Experimental results were analysed with vogit viscoelaticity model based on pioneering theory of Chein et al. The results showed that the erythrocyte membrane elastic moduli ((6.970 +/- 1.050) x 10(-3) dyn/cm) and viscous coefficients ((0.936 +/- 0.242) x 10(-4) dyn x s/cm) of the cor pulmonale patients was significantly higher than those of the normal subjects ((5.203 +/- 1.051) X 10(-3) dyn/cm, (0.620 +/- 0.053) x 10(-4) dyn x s/cm). The membrane elastic moduli, viscous coefficients, rigidity of erythrocyte, and viscosity were all increased. It may be the important subcellular mechanism to cause the decrease of erythrocyte deformability and hyperviscosity of blood in these patients.
Blood Viscosity ; Elasticity ; Erythrocyte Deformability ; physiology ; Erythrocyte Membrane ; physiology ; Erythrocytes ; physiology ; Humans ; Models, Biological ; Pulmonary Heart Disease ; blood