Objective To investigate the clinical significance of dynamic changes in the expression levels of tumor associated autoantibody(TAAB),including tumor protein 53(p53),protein gene product 9.5(PGP9.5),SRY-box containing gene 2(SOX2),G antigen 7 ATP-dependent RNA helicase(GAGE7),ATP-dependent RNA helicase(GBU4-5),melanoma antigen A1(MAGE A1),cancer/testis antigen(CAGE)in non-small cell lung cancer(NSCLC)patients before and after surgery.Methods Forty-one patients with NSCLC who underwent surgical treatment at the Department of Thoracic Surgery of Li Huili Hospital,Ningbo Medical Center,were selected from January 2019 to August 2022.Enzyme linked immunosorbent assay(ELISA)was used to detect the expression levels of seven TAAB markers in the serum of 41 NSCLC patients undergoing surgical treatment.Results The expression levels of PGP9.5 were found to be significantly higher in the squamous cancer group than in the adenocarcinoma group(P<0.05),while the expression levels of GAGE7 were found to be significantly higher in the adenocarcinoma group than in the squamous cancer group(P<0.05).The expression level of GBU4-5 was found to be significantly higher in the squamous carcinoma group than in the adenocarcinoma group at one week after surgery(Z=-2.095,P=0.036).Within the adenocarcinoma group,the expression levels of GBU4-5(P=0.027)and CAGE(P=0.042)were found to be significantly lower at one week after surgery compared with those before surgery.The expression levels of PGP9.5(P=0.004),GAGE7(P<0.001),and GBU4-5(P=0.014)were found to have decreased significantly at six months postoperatively in comparison to their preoperative levels.In the squamous cancer group,no statistically significant differences were observed in the expression levels at one week after surgery.The expression level of SOX2(P=0.011)exhibited a statistically significant decrease at six months postoperatively in comparison to the preoperative levels.Similarly,the expression level of GBU4-5 was found to be significantly decreased at one week postoperatively in comparison to the preoperative levels(P=0.009).The expression levels of PGP9.5(P=0.003),GAGE7(P=0.001),and GBU4-5(P=0.013)were found to have decreased significantly at six months postoperatively.Conclusion Surgical intervention can reduce the expression level of 7 TAABs in NSCLC patients.Monitoring 7 TAABs before and after surgical treatment is helpful to predict the surgical efficacy and postoperative disease progression of NSCLC patients.

Objective To summarize the experience of multi-disciplinary team(MDT)in the pediatric department of Qujing Maternal and Child Health Hospital,and to evaluate the effectiveness of MDT on neonatal brain injury.Methods The clinical data of children with brain injury and treated in the pediatrics department of Qujing Maternal and Child Health Hospital from November 2019 to April 2023 were collected.The children with brain injury and treated from October 2019 to June 2020 were regarded as the non-MDT group,and the children with brain injury and treated from July 2020 to April 2023 were regarded as the MDT group for comparative analysis.Chi-square test/t-test was used to compare and analyze the clinical data of the two groups.Results Among the 890 cases of pediatric brain injury,there were 519 males and 371 females.The median and quartiles of the age distribution for the two groups were as follows:MDT group 2.00(0.82,5.00)years and non-MDT group 1.00(1.00,4.00)years.Craniocerebral injury was the main type of brain injury in both groups,in addition,among children with craniocerebral injury and intracranial hemorrhage,the cure rate of MDT group was higher than that of non-MDT group,and the difference was statistically significant(P<0.05).Among the 405 children in MDT group,154(38.0%)underwent the surgery,while among the 485 children in non-MDT group,121(24.9%)underwent the surgery.The difference was statistically significant(P<0.05).23.2% of children in MDT group were in critical condition during the hospitalization,which was significantly lower than that in non-MDT group(30.5%),and the difference was statistically significant(P<0.05).The unhealed rate of MDT group(2.0%)was also significantly lower than that of non-MDT group(5.6%),the cure rate of MDT group(40.5%)was significantly higher than that of non-MDT group(34.4%),and there was a statistically significant difference(P<0.05).The expense of treatment,medicine and sanitary materials in MDT group were lower than those in non-MDT group,and the differences were statistically significant(P<0.05).The multivariate Logistic regression model analysis of the cure rate of children with brain injury showed that the MDT model could effectively improve the cure rate of children with brain injury(RR = 1.513,95% CI = 1.134-2.020).The results of multiple linear regression model analysis showed that there was no statistical difference in the effect of MDT on the actual hospitalization days of children(P>0.05).Conclusion Using MDT model to diagnose and treat children with brain injury is helpful to improve the cure rate,reduce the risk of children's disease aggravation,and achieve the significant therapeutic effects in children with brain injury.MDT model is worth popularizing and applying in children with brain injury.

OBJECTIVE:There is no consensus on which sit-up strategy to adopt in knee osteoarthritis patients of different ages.Therefore,this study evaluated the biomechanical characteristics of sit-ups in knee osteoarthritis patients of different ages compared with healthy individuals by meta-analysis system and analyzed the sit-up movement patterns of patients of different ages to provide a reference for improving the sit-up function of patients. METHODS:By March 2023,observational studies of biomechanical characteristics of sitting up in patients with knee osteoarthritis and healthy population were retrieved on PubMed,Web of Science and CNKI.Subjects were required to be patients over 50 years of age with knee osteoarthritis who had Kellgren-Lawrence severity grading≥Ⅰ on knee imaging and who had regular knee pain.Subjects were analyzed by age(50-60 years vs.over 60 years)and severity(mild to moderate patients vs.severe patients)subgroups according to inclusion and exclusion criteria.Quality assessment was performed using the modified Down and black scale.Stata 16.0 software was used to perform subgroup analysis to determine the biomechanical characteristics of sitting up in patients with knee osteoarthritis of different ages and severities. RESULTS:A total of 14 randomized controlled trials(824 subjects)were included in the meta-analysis.The mean quality score of all included literature was 76.2,with a range of 66.7 to 86.7,all of which were of medium to high quality and representative.The included studies were of moderate to high quality and representative.Meta-analysis results found that(1)compared to healthy individuals,patients with knee osteoarthritis had longer total sitting up time(SMD=0.92,95%CI:0.76-1.09),P<0.001)and longer extension phase time(SMD=0.46,95%CI:0.18-0.74,P=0.001).Compared to mild to moderate patients,the total duration increased more significantly in severe patients(P<0.001)and the duration of the extension phase increased more significantly in patients over 60 years of age than in patients 50-60 years of age(P=0.001).(2)Compared to healthy individuals,patients with knee osteoarthritis had greater sitting-up trunk flexion motion range(SMD=0.64,95%CI:0.37-0.91,P<0.001);knee flexion motion range(SMD=-0.47,95%CI:-0.70 to-0.24,P<0.001)and ankle dorsiflexion motion range(SMD=-0.32,95%CI:-0.56 to-0.08,P=0.01)were smaller.And knee flexion motion range decreased more significantly in patients over 60 years of age than in patients 50-60 years of age(P<0.001).(3)The peak hip flexion moment(SMD=-0.57,95%CI:-0.83 to-0.31,P<0.001)and peak knee extension moment(SMD=-0.83,95%CI:-1.08 to-0.59,P<0.001)were smaller in patients with knee osteoarthritis. CONCLUSION:(1)Patients with knee osteoarthritis over the age of 60 years have a longer sit-up cushion and extension phase than patients aged 50 to 60 years.The increase in total sit-up duration was also more pronounced in patients with higher severity grades.The increased length of sitting up in patients with advanced age and knee osteoarthritis severity may increase the duration of cartilage loading,exacerbate knee pain symptoms,and increase the difficulty of sitting up in this population.(2)Patients with knee osteoarthritis exhibit limited knee and ankle flexion motion range.Knee mobility is more limited in patients over 60 years of age.(3)Patients with knee osteoarthritis have reduced peak hip flexion and knee extension moments,which may be a compensatory strategy for pain relief.

Objective:To evaluate a novel distraction reductor in the surgical treatment of unstable distal radius fractures with metaphyseal volar comminution.Methods:From January 2019 to December 2020, 27 patients with unstable distal radius fracture complicated with metaphyseal volar comminution were treated at Department of Orthopaedics, Zhoupu Hospital Affiliated to Shanghai University of Medicine & Health Sciences. They were 6 males and 21 females, with an age of (69.4±9.4) years. All fractures were unilateral and closed, involving the right side in 17 cases and the left side in 10 cases. All patients were treated by internal fixation with an anatomical locking plate through the volar approach and the novel distraction reductor was used to reduce the fracture ends. Regular imaging examinations were performed to evaluate the reduction, maintenance and union of fractures after surgery. One year after operation, the curative efficacy was assessed by evaluation of the range of wrist motion, Disabilities of the Arm, Shoulder and Hand (DASH) score, Gartland-Werley score and Bartra radiology score.Results:All the operations went on successfully with a duration of (92.3±8.9) min. All the incisions healed primarily. The follow-up time was (15.9±2.9) months. The radial height, palmar tilt, ulnar inclination and articular surface step-off immediately after operation [(11.23±1.51) mm, 12.10°±3.44°, 20.54°±3.44°, and (0.95±0.42) mm] were not significantly lost compared with those one year after operation [(11.22±1.55) mm, 12.07°±3.44°, 20.51°±3.33°, and (0.93±0.40) mm] (all P>0.05). One year after operation, the range of wrist motion was good with dorsiflexion of 59.7°±5.5°, palm flexion of 63.0°±9.1°, pronation of 66.5°±5.5°, supination of 61.2°±5.6°, radial deviation of 22.7°±4.8°, and ulnar deviation of 30.3°±6.1°; DASH score was 13.5±5.5; Bartra radiology score was 88.6±6.5, giving an excellent and good rate of 88.9% (24/27);Gartland-Werley score was 2.7±2.1, giving an excellent and good rate of 92.6% (25/27). Follow-ups observed no poor fracture healing, internal fixation failure, tendon or nerve injury or traumatic arthritis. Conclusion:In the surgical treatment of unstable distal radius fractures with metaphyseal volar comminution, the novel distraction reductor can lead to ideal reduction of displaced fractures and effectively correct the shortening caused by volar cortex comminution to achieve satisfactory functional effects in clinic.

OBJECTIVE To explore the detection capability of nanoparticle tracking analysis(NTA) and nano-flow cytometry(nanoFCM) in detecting standard microspheres and adipose mesenchymal stem cells(AdMSCs) derived extracellular vesicles(EVs), provide experimental basis for the identification and quality control of EVs. METHODS Firstly, the detection capabilities of NTA and nanoFCM were verified by detecting standard microspheres. For the AdMSCs-EVs produced via PEG6000-based ultracentrifugation method, the general characterizations were performed with Western blotting and transmission electron microscope. The particle size distributions as well as the post-PKH67 labeling positive EV ratios were analyzed by NTA and nanoFCM respectively. RESULTS NTA and nanoFCM had similar capacity regarding to particle concentration detection, while nanoFCM had higher accuracy and better particle size differentiation ability. The EVs size distribution results showed that, samples analyzed by NTA presented a wider distribution while nanoFCM presented a narrower distribution. For detection of PKH67-positive EVs, the positive ratios detected by NTA were significantly lower than by nanoFCM. CONCLUSION NanoFCM has higher accuracy along with some application limitations. NTA is relatively less capable at particle size distinction, but has a wider particle size detection range. Therefore, for detection of EVs, NTA can satisfy the needs of size distribution characterization, while nanoFCM with high sensitivity for fluorescence detection is more suitable for analyzing the proportion of surface markers in EVs.

Objective:To systematically evaluate the influencing factors on unplanned shutdown of continuous blood purification, and to provide reference basis for the prevention of unplanned shutdown.Methods:The literatures related to the influencing factors of unplanned shutdown of continuous blood purification in CNKI, Wanfang Database, Chinese Biomedical Literature Database, Chinese Science and Technology Periodical Full-text Database, PubMed and Web of Science were searched.The retrieval time of Chinese database was from the establishment of the database to March 2021.English databases were searched from March 2016 to March 2021.Literature selection, quality evaluation and data extraction were independently conducted by two researchers, and Meta-analysis was performed by Stata 14.0 software.Results:A total of 11 studies were included, including 3 031 cases of continuous blood purification treatment and 1 412 cases of unplanned discontinuation.The combined OR value and 95% CI of all influencing factors were as follows: treatment mode 2.22 (1.06-4.62), blood flow velocity 0.91 (0.776-1.09), agitation 4.54 (2.33-8.86), ventilator 2.67 (1.63-4.38), transfusing blood products and fat milk 1.07 (0.34-3.36), one-time catheter success 0.26 (0.05-1.42), catheterization site (femoral vein vs.jugular vein) 2.24 (0.83-6.02). Conclusion:Unplanned deplaning is influenced by many factors.Treatment mode, agitation and ventilator use are the risk factors for unplanned deplaning.There is no correlation between blood flow velocity, transfusing blood products and fat milk, one-time catheterization success, catheterization site and unplanned deplaning.

OBJECTIVE: To investigate the clinical outcomes of patients with aneurysmal subarachnoid hemorrhage (aSAH) after surgeries in Yunnan Province. METHODS: We retrospectively analyzed the demographic features, vascular risk factors, severity at admission, and aneurysm locations in 85 patients with aSAH receiving surgical interventions in Yunnan Province. All the patients were treated by aneurysm clipping or coiling and followed up for clinical outcomes and recovery of daily activities evaluated by modified Rankin Scale (mRS) and Activities of Daily Living (ADL) scale, respectively. RESULTS: Thirty-four of the patients (40.0%) underwent aneurysm clipping and 51 (60.0%) underwent aneurysm coiling. During a median follow- up period of 66.23 months (IOR, 12.03 months), 84.7% of the patients had low mRS scores, and 78.8% lived independently. The WFNS grade at admission was significantly correlated with the follow-up mRS scores (95%: 1.48-19.09, =0.011) and ADL (95%: 2.55-28.77, < 0.001). Multivariate analysis showed that age (95%: 1.02-1.23, =0.017; 95%: 1.00-1.15, =0.038) and a high WFNS grade at admission (95%: 2.19-141.48, =0.007; 95%: 2.84-82.61, =0.002) were independent predictors of both mRS and ADL scores at follow-up. There was no significant difference in clinical outcomes or the length of hospital stay between the two treatment strategies ( > 0.05), but the cost of hospitalization was significantly higher in coiling group than in the clipping group ( < 0.001). CONCLUSIONS Both aging and a high WFNS grade at admission are associated with a poor prognosis of aSAH, for which aneurysm clipping and coiling have similar long- term outcomes, but for patients with a high WFNS score, aneurysm clipping is favored over coiling in terms of health economics.

Objective: To explore the mediating effect of self-efficacy between mindfulness and self-esteem. Methods: In this study, 400 junior school students from a middle school in Jiangxi Province was recruited and complete mindful self scale, general self-efficacy scale and self-esteem scale. Results: The scores of mindfulness, self-efficacy, self-esteem were (63.89±9.12), (22.82±4.83) and (26.14±4.43), respectively. Mindfulness and its four dimensions (self-insight, self-acceptance, self-compassion and non-persistence) were significantly positively correlated with self-esteem (r=0.18-0.53, all P<0.01). Mindfulness and its three dimensions (self-insight, self-compassion and non-persistence) were significantly positively correlated with self-efficacy (r=0.22-0.39, all P<0.01). Self-efficacy and self-esteem were significantly positively correlated (r=0.82, P<0.01). Mindfulness positively predicted self-efficacy (β=0.38, P<0.01), self-efficacy positively predicted self-esteem (β=0.74, P<0.01), and mindfulness positively predicted self-esteem (β=0.25, P<0.01). That was, self-efficacy partially mediated the relationship between mindfulness and self-esteem. The mediating effect value was 0.28, accounting for 52.83% of the total effect. Conclusion Mindfulness not only directly affects adolescents' self-esteem, but also influences adolescents' self-esteem through self-efficacy.

The Asian Society of Gynecologic Oncology International Workshop 2014 on gynecologic oncology was held in Asan Medical Center, Seoul, Korea on the 23rd to 24th August 2014. A total of 179 participants from 17 countries participated in the workshop, and the up-to-date findings on the management of gynecologic cancers were presented and discussed. This meeting focused on the new trends in the management of cervical cancer, fertility-sparing management of gynecologic cancers, surgical management of gynecologic cancers, and recent advances in translational research on gynecologic cancers.
Female ; Fertility Preservation/methods ; Genital Neoplasms, Female/*therapy ; Humans ; Ovarian Neoplasms/therapy ; Translational Medical Research/methods ; Uterine Cervical Neoplasms/therapy

Objective To study the neuroprotective effect of apigenin on microglia which was exposed to oxygen glucose deprivation and reperfusion (OGD/R), which was characterized by its influence on IL-1? and TNF-? expression. Methods Primary microglial cultures were prepared from newborn rat brain. The purity of isolated cells were identified by GSA-IB4. The cells were randomized into 5 groups:normal group, DMSO group and apigenin-treated groups (10, 25, 50 ?mol/L). The cells of DMSO group and apigenin-treated group were exposed to 8 h of OGD and 24 h of reperfusion in the presence or absence of apigenin at a range of concentrations. Culture supernatants were collected and IL-1? and TNF-? were detected by ELISA assay. Results The expression of IL-1? and TNF-? were significantly higher in DMSO group (P