Objective To analyze the research progress of acupuncture and moxibustion in the treatment of simple obesity,thus to provide reference for further research in this field.Methods The relevant literature in China National Knowledge Infrastructure(CNKI)and Wanfang Data Knowledge Service Platform in the past 24 years were retrieved,and analysis of authors,institutions,and keywords were carried out by CiteSpace 6.2.R6 software,and visualization maps were drawn.Results A total of 889 valid articles were included,and the number of annual publications tended to be stable;the authors in this field were Zhou Zhongyu and Chen Xia as the core author team;the most involved research institution in the institutional cooperation map was the Nanjing University of Chinese Medicine;the high-frequency keywords mainly concentrated on"acupuncture therapy","acupuncture and muxibustion therapy","simple obesity","acupoint catgut embedding therapy",etc.;the keyword clusters covered a total of 13 items,namely"electroacupuncture","acupuncture","acupuncture and moxibustion","acupoint catgut embedding",etc.Conclusion There are many studies on acupuncture and moxibustion in the treatment of simple obesity,and the hot spots of the study are focused on small-sample clinical observation,literature research,and research on the mechanism of action.In the future,researchers can conduct further high-quality studies as well as research on the application and mechanism of action of specific acupuncture therapies,with the aim of developing the preferable therapeutic approaches of acupuncture for the treatment of simple obesity.

Based on the theory of"body fluid and blood sharing the same origin",this paper reveals that the core pathogenesis of atopic dermatitis(AD)lies in the abnormal distribution and transformation of body fluid and blood,which is closely related to dysfunction of the lung,spleen,and kidney.Integrating modern medical research on immune regulation and skin barrier function,we propose that in the early stage of AD,due to deficient endowment,acquired loss of nutrition,insufficiency of the spleen and kidney,and failure of the lung-defense system to protect,external pathogens stagnate in the fleshy exterior.This leads to dysfunction of the lung's governance over the skin,impaired distribution of body fluid,obstruction of qi and blood circulation,and stagnation in the skin,resulting in exudative dampness.The dampness stagnates and transforms into heat.Acupuncture should primarily focus on clearing the lung,dispersing stagnation,distributing body fluid,and unblocking collaterals.In the middle and late stages,as lung disease affects the spleen and kidney,acupuncture should emphasize supplementing and tonifying the spleen and kidney,nourishing blood,and moistening dryness,while simultaneously addressing both the root and the branch.For intractable pruritus,fire needling or pricking-collateral cupping can be employed to control itching and reduce recurrence.

Fat emulsion is a drug commonly used clinically for parenteral nutrition support in critically ill patients.With the development of the pharmaceutical industry, fat emulsion has formed a variety of different formulations, among which different types of fat emulsion have their own metabolic and body energy supply characteristics, and the application indications are also different. In addition to providing the supply of nutrients, the role of fat emulsion in anti-toxicity, immune regulation, anti-inflammatory, anti-shock, cardiopulmonary resuscitation and other aspects has gradually been discovered. This article reviews the existing evidence-based medical evidence and expounds the mechanism and therapeutic role of fat emulsion in the treatment of critically ill patients with poisoning. Its value in the treatment of critically ill patients with poisoning was discussed, and some references were provided for the application of non-nutritional functions of fat emulsion in the future.

Fat emulsion is a drug commonly used clinically for parenteral nutrition support in critically ill patients.With the development of the pharmaceutical industry, fat emulsion has formed a variety of different formulations, among which different types of fat emulsion have their own metabolic and body energy supply characteristics, and the application indications are also different. In addition to providing the supply of nutrients, the role of fat emulsion in anti-toxicity, immune regulation, anti-inflammatory, anti-shock, cardiopulmonary resuscitation and other aspects has gradually been discovered. This article reviews the existing evidence-based medical evidence and expounds the mechanism and therapeutic role of fat emulsion in the treatment of critically ill patients with poisoning. Its value in the treatment of critically ill patients with poisoning was discussed, and some references were provided for the application of non-nutritional functions of fat emulsion in the future.

Objective:To investigate whether microRNA-515-5p (miR-515-5p) can target the endogenous lectin 2 (ITLN2) to affect the proliferation, migration and invasion of endometrial stromal cells (ESCs) in endometriosis (EMT).Methods:Eutopic and ectopic endometrium tissues of 24 patients with EMT who attended Department of Reproductive Medicine, the Affiliated Hospital of Jining Medical College for treatment from December 2020 to December 2021, and normal endometrium tissues of 18 non-EMT patients who underwent laparoscopic surgery were selected. RT-qPCR was used to detect the relative expression of miR-515-5p and ITLN2 mRNA, and Western blotting was used to detect ITLN2 protein expression in endometrium tissues. ESCs from EMT patients were isolated and cultured in primary culture. ESCs were transfected with Lipofectamine 2000 reagent, and were divided into blank group, miR-515-5p inhibitor group, miR-515-5p inhibitor negative control (NC) group. Cell proliferation was detected by CCK-8 method. The ability of cell migration and invasion was evaluated by scratch healing and Transwell test. The target relationship between miR-515-5p and ITLN2 was detected by double luciferase reporter gene. RT-qPCR was used to detect the relative expression of miR-515-5p and ITLN2 mRNA in each group. Western blotting was used to detect the relative expression of ITLN2, interleukin (IL)-1β, IL-6 and tumor necrosis factor-α (TNF-α) protein. Results:The relative expression of miR-515-5p in EMT eutopic and ectopic endometrium was significantly higher than that in normal endometrium (both P<0.001). The ITLN2 mRNA level in EMT eutopic and ectopic endometrium was significantly lower than that in normal endometrium, and ITLN2 mRNA level in ectopic endometrium was significantly lower than that in eutopic endometrium (all P<0.001). The level of ITLN2 protein in EMT ectopic endometrium was significantly lower than that in EMT eutopic endometrium and normal endometrium (both P<0.001), and the protein level of ITLN2 in EMT eutopic endometrium was significantly lower than that in normal endometrium ( P<0.001). In miR-515-5p inhibitor group, the cell survival rate [(54.71±2.78)%], the scratch healing rate [(38.31±3.78)%], invasion number (286.67±25.15), the relative expressions of miR-515-5p, IL-1β, IL-6 and TNF-α were significantly lower than those of the blank group [(100.00±0.00)%, P<0.001; (84.08±5.14)%, P<0.001; 515.67±22.19, P<0.001; P<0.001; P=0.012; P=0.010; P<0.001], the relative expression levels of ITLN2 mRNA and protein were significantly higher than those in the blank group ( P<0.001; P<0.001). Through bioinformatics analysis and double luciferase reporter gene detection, ITLN2 was a potential target gene of miR-515-5p. Conclusion:Lowering the level of miR-515-5p can inhibit the proliferation, migration and invasion of ESCs in EMT patients, which may be related to the targeted increase of ITLN2 expression, thereby reducing inflammatory factors level.

Objective:To investigate whether microRNA-515-5p (miR-515-5p) can target the endogenous lectin 2 (ITLN2) to affect the proliferation, migration and invasion of endometrial stromal cells (ESCs) in endometriosis (EMT).Methods:Eutopic and ectopic endometrium tissues of 24 patients with EMT who attended Department of Reproductive Medicine, the Affiliated Hospital of Jining Medical College for treatment from December 2020 to December 2021, and normal endometrium tissues of 18 non-EMT patients who underwent laparoscopic surgery were selected. RT-qPCR was used to detect the relative expression of miR-515-5p and ITLN2 mRNA, and Western blotting was used to detect ITLN2 protein expression in endometrium tissues. ESCs from EMT patients were isolated and cultured in primary culture. ESCs were transfected with Lipofectamine 2000 reagent, and were divided into blank group, miR-515-5p inhibitor group, miR-515-5p inhibitor negative control (NC) group. Cell proliferation was detected by CCK-8 method. The ability of cell migration and invasion was evaluated by scratch healing and Transwell test. The target relationship between miR-515-5p and ITLN2 was detected by double luciferase reporter gene. RT-qPCR was used to detect the relative expression of miR-515-5p and ITLN2 mRNA in each group. Western blotting was used to detect the relative expression of ITLN2, interleukin (IL)-1β, IL-6 and tumor necrosis factor-α (TNF-α) protein. Results:The relative expression of miR-515-5p in EMT eutopic and ectopic endometrium was significantly higher than that in normal endometrium (both P<0.001). The ITLN2 mRNA level in EMT eutopic and ectopic endometrium was significantly lower than that in normal endometrium, and ITLN2 mRNA level in ectopic endometrium was significantly lower than that in eutopic endometrium (all P<0.001). The level of ITLN2 protein in EMT ectopic endometrium was significantly lower than that in EMT eutopic endometrium and normal endometrium (both P<0.001), and the protein level of ITLN2 in EMT eutopic endometrium was significantly lower than that in normal endometrium ( P<0.001). In miR-515-5p inhibitor group, the cell survival rate [(54.71±2.78)%], the scratch healing rate [(38.31±3.78)%], invasion number (286.67±25.15), the relative expressions of miR-515-5p, IL-1β, IL-6 and TNF-α were significantly lower than those of the blank group [(100.00±0.00)%, P<0.001; (84.08±5.14)%, P<0.001; 515.67±22.19, P<0.001; P<0.001; P=0.012; P=0.010; P<0.001], the relative expression levels of ITLN2 mRNA and protein were significantly higher than those in the blank group ( P<0.001; P<0.001). Through bioinformatics analysis and double luciferase reporter gene detection, ITLN2 was a potential target gene of miR-515-5p. Conclusion:Lowering the level of miR-515-5p can inhibit the proliferation, migration and invasion of ESCs in EMT patients, which may be related to the targeted increase of ITLN2 expression, thereby reducing inflammatory factors level.

Infertility is gradually becoming a major problem affecting health worldwide, and male factors also play an important role in infertility. Extracellular vesicles (EVs) are ultramicro membranous vesicles released by cells during activation or apoptosis, which play an important role in cell communication. Relevant studies have shown that extracellular vesicles contain a variety of bioactive substances and participate in infertility related pathophysiological processes by influencing the content of intercellular transmission. Therefore, we reviewed the relationship between extracellular vesicles and male infertility, and expounded the occurrence and potential treatment of male infertility from another perspective.

Objective:To investigate the effectiveness and safety of endovascular therapy for acute progressive stroke caused by large vessel occlusion (LVO).Methods:Patients with progressive stroke caused by LVO admitted to the Department of Neurology, Yueyang Central Hospital from January 2019 to February 2022 were retrospective included. Patients with an Alberta Stroke Program Early CT Score (ASPECTS) or posterior circulation ASPECTS (pc-ASPECTS) ≥6 after progression were selected for endovascular therapy, including mechanical thromboectomy, thrombus aspiration, balloon angioplasty, stenting, or a combination of the above methods. Patients in the time window (anterior circulation within 12 h and posterior circulation within 24 h) and outside the time window (anterior circulation >12 h, posterior circulation >24 h) as well as those in the anterior and posterior circulation were compared.Results:A total of 20 patients with progressive stroke caused by LVO received endovascular treatment were included. There were 17 males and 3 females, aged 59.45±12.06 years. Three patients (15%) developed asymptomatic intracranial hemorrhage, and 12 (60%) had a good outcome 3 months after procedure. There were no statistically significant differences in the rate of successful vascular recanalization, incidence of intracranial hemorrhage, and the rate of poor outcomes between patients within and outside the time window and between the patients with anterior and posterior circulation.Conclusion:Endovascular therapy may be safe and effective for patients with progressive stroke caused by LVO with ASPECTS or pc-ASPECTS scores ≥6.

Objective:To summarize the clinical characteristics and treatment effect of patients with acute oral 84 disinfectant poisoning, so as to improve the understanding, diagnosis and treatment of the disease.Methods:In January 2022, 25 hospitalized patients with acute oral 84 disinfectant poisoning admitted to our department from March 2016 to August 2021 were selected as the research objects, and their general conditions, poisoning reasons, poisoning time, dose of poisoning, clinical manifestations, blood routine and biochemical indicators, diagnosis, treatment and prognosis were selected.Results:A retrospective analysis was performed. Among the 25 patients, there were 4 males and 21 females, aged from 20 to 91 years, and M ( Q1, Q3) was 38.7 (27, 46) years; The poisoning time (from exposure to poison to treatment) was 1~72 h, and M ( Q1, Q3) was 10.5 (3, 11.5) h. The length of stay was 1~20 days, and M ( Q1, Q3) was 5.72 (2, 7) days.The dose was 40-500 ml, and the M ( Q1, Q3) was 219.6 (100, 330) ml. Chest CT showed exudative changes in both lungs in 4 patients, excessive decreased permeability in 1 case and pleural effusion in 1 case. Gastroscope showed 2 cases of erosive inflammation of gastric body and antrum, 1 case of esophageal ulcer and cardiac ulcer, 1 case of corrosive gastritis, gastric fundus ulcer and esophageal stenosis. Abdominal X-ray showed 1 case of abdominal intestinal dilatation and pneumatosis with multiple gas-liquid planes.There were 1 case of type I respiratory failure, 6 cases of gastrointestinal bleeding and 1 case of incomplete intestinal obstruction. There were 19 cases of nausea and vomiting, 9 cases of abdominal pain, 6 cases of pharyngeal pain and 6 cases of retrosternal burning pain, 1 case of cough and 2 cases of fatigue. Conclusion:Acute oral 84 disinfectant will cause varying degrees of damage to the human digestive tract and lungs. In severe cases, gastrointestinal bleeding, intestinal obstruction, hypoxemia, etc, and even life-threatening, should be paid attention to clinically. The treatment is mainly symptomatic support treatment, such as protecting gastrointestinal mucosa, controlling acute inflammatory reaction, protecting the functions of liver and kidney and other important organs.

Objective:To summarize the clinical characteristics and treatment effect of patients with acute oral 84 disinfectant poisoning, so as to improve the understanding, diagnosis and treatment of the disease.Methods:In January 2022, 25 hospitalized patients with acute oral 84 disinfectant poisoning admitted to our department from March 2016 to August 2021 were selected as the research objects, and their general conditions, poisoning reasons, poisoning time, dose of poisoning, clinical manifestations, blood routine and biochemical indicators, diagnosis, treatment and prognosis were selected.Results:A retrospective analysis was performed. Among the 25 patients, there were 4 males and 21 females, aged from 20 to 91 years, and M ( Q1, Q3) was 38.7 (27, 46) years; The poisoning time (from exposure to poison to treatment) was 1~72 h, and M ( Q1, Q3) was 10.5 (3, 11.5) h. The length of stay was 1~20 days, and M ( Q1, Q3) was 5.72 (2, 7) days.The dose was 40-500 ml, and the M ( Q1, Q3) was 219.6 (100, 330) ml. Chest CT showed exudative changes in both lungs in 4 patients, excessive decreased permeability in 1 case and pleural effusion in 1 case. Gastroscope showed 2 cases of erosive inflammation of gastric body and antrum, 1 case of esophageal ulcer and cardiac ulcer, 1 case of corrosive gastritis, gastric fundus ulcer and esophageal stenosis. Abdominal X-ray showed 1 case of abdominal intestinal dilatation and pneumatosis with multiple gas-liquid planes.There were 1 case of type I respiratory failure, 6 cases of gastrointestinal bleeding and 1 case of incomplete intestinal obstruction. There were 19 cases of nausea and vomiting, 9 cases of abdominal pain, 6 cases of pharyngeal pain and 6 cases of retrosternal burning pain, 1 case of cough and 2 cases of fatigue. Conclusion:Acute oral 84 disinfectant will cause varying degrees of damage to the human digestive tract and lungs. In severe cases, gastrointestinal bleeding, intestinal obstruction, hypoxemia, etc, and even life-threatening, should be paid attention to clinically. The treatment is mainly symptomatic support treatment, such as protecting gastrointestinal mucosa, controlling acute inflammatory reaction, protecting the functions of liver and kidney and other important organs.