Objective To investigate the surgical treatment strategy of intracranial alveolar echinococcosis(AE)and the clinical outcomes.Methods The clinical and follow-up data of 15 intracranial AE patients who underwent surgical treatment in the Departments of Neurosurgery of Sichuan Provincial People's Hospital(SPPH)and People's Hospital of Aba Tibetan and Qiang Autonomous Prefecture(a branch hospital of SPPH)between March 2017 and January 2021 were retrospectively analyzed.Full follow-up data were available for each of the 15 cases.The clinical and imaging characteristics,general surgical information,and surgical outcomes were analyzed.Results In the 15 patients,there were a total of 50 intracranial lesions,with an average of(3.3±3.1)/case.Four cases had solitary intracranial lesions,while 11 cases had multiple lesions,with the number of intracranial lesions per case ranging from 2 to 13.All patients with solitary intracranial lesions received total resection.In 6 patients with multiple intracranial lesions,only the largest lesion was surgically removed,and in 5 patients,2 to 3 adjacent lesions were surgically removed.All but one patient had extracranial lesions in their liver,lungs,kidneys,adrenal glands,and thoracic vertebrae.The patients were followed up for 12 to 58 months after surgery,with the mean follow-up time being(28.1±13.4)months.Among the 15 cases,13 showed stable intracranial condition during postoperative follow-up.Intracranial lesions recurred in 2 patients who had deep lesions accompanied by dissemination to the subarachnoid space.Two patients died during follow-up.Conclusion Microsurgical treatment of intracranial AE is effective,but total surgical resection is difficult to accomplish when patients have echinococcosis lesions located at a depth,especially when the lesions are spreading to the subarachnoid space.The prognosis of patients is closely associated with the extent of lesion invasion and the control of systemic hydatid lesions,especially those in the liver.

Objective The effect of different doses of ethylnitrosourea(ENU)and cyclophosphamide(CP)on the loss rate of CD59 on peripheral blood erythrocytes was explored to optimize the detection method of Pig-a gene mutation. Methods According to the weight and loss rate of CD59 on peripheral blood erythrocytes,rats were divided into 4 groups:the control group,CP 40 mg/kg group,ENU 10 mg/kg group and ENU 40 mg/kg group(n=6). The control group was injected i.p. with PBS,other groups were injected i.p. with corresponding solutions. The body weight of rats on days 0,7,14,21, 28, 42 and 56 were recorded. At the same time, blood samples were collected and incubated with antibodies,and the loss rate of RBCCD59-was detected by flow cytometry. Results Compared with the control group, at different time points, the body weight and weight gain of ENU 10 mg/kg group and ENU 40 mg/kg group had no statistically significant difference(P > 0.05),while those in the CP 40 mg/kg group were significantly decreased(P <0.05). The loss rate of RBCCD59-was significantly increased in the CP 40 mg/kg group at 28,42 and 56 days, ENU 10 mg/kg group at 42 and 56 days,and ENU 40 mg/kg group at 7,14,21,28,42 and 56 days,(P < 0.05). The results showed a dose-response relationship. Conclusions Under the conditions of this Pig-a mutation detection method,ENU is superior to CP on raising loss rate of RBCCD59-,ENU 40 mg/kg is better than 10 mg/kg,and 28 days is suitable as the test period.