Pulmonary fibrosis（PF） is a progressive and life-threatening disease with limited therapeutic options， highlighting the urgent need for innovative drug discovery strategies. To address this challenge， the authors propose the formula-originated rational intelligent screening&translation（FIRST）， a systematic framework for developing anti-fibrotic monomers derived from classical traditional Chinese medicine（TCM）. The strategy integrates three key dimensions， including tissue-oriented intelligent screening of active compounds， structural optimization based on drug-target spatial interactions and plant biosynthetic pathways， and cross-scale validation of drug. We further highlight its applications in discovering tissue-oriented novel drugs from clinically validated TCM， the development and mechanistic elucidation of anti-fibrotic therapeutics， as well as the clinical translation and secondary development of candidate drugs. This strategy paves the way for first-in-class， formula-derived monomeric drugs with defined structures， clarified mechanisms， and proven safety， offering a transformative avenue to meet the urgent therapeutic needs of PF and setting a new paradigm for TCM-based drug innovation.

Pulmonary fibrosis（PF） is a progressive and life-threatening disease with limited therapeutic options， highlighting the urgent need for innovative drug discovery strategies. To address this challenge， the authors propose the formula-originated rational intelligent screening&translation（FIRST）， a systematic framework for developing anti-fibrotic monomers derived from classical traditional Chinese medicine（TCM）. The strategy integrates three key dimensions， including tissue-oriented intelligent screening of active compounds， structural optimization based on drug-target spatial interactions and plant biosynthetic pathways， and cross-scale validation of drug. We further highlight its applications in discovering tissue-oriented novel drugs from clinically validated TCM， the development and mechanistic elucidation of anti-fibrotic therapeutics， as well as the clinical translation and secondary development of candidate drugs. This strategy paves the way for first-in-class， formula-derived monomeric drugs with defined structures， clarified mechanisms， and proven safety， offering a transformative avenue to meet the urgent therapeutic needs of PF and setting a new paradigm for TCM-based drug innovation.

Objective:To analyze the clinical features of elderly patients with hip fracture and the expression of serum vitamin D (VitD), 25- hydroxy vitamin D (25-OH-D) and bone mineral density,, BMD) .Methods:Collect clinical data from 90 elderly patients with hip fractures admitted to the Emergency Department of Fenyang Hospital in Shanxi Province from Oct. 2020 to Dec. 2023 for analysis. Based on whether the patients have VitD deficiency, they were divided into group A (severe deficiency of 25-OH-D, insufficient) and group B (sufficient 25-OH-D). Clinical characteristics of patients and the levels of VitD, 25-OH-D, and BMD, and the correlation between the above indicators were analyzed.Results:The age of patients in groups A and B, whether there is osteoporosis, and 25-OH-D levels are suspicious influencing factors that cause VitD deficiency ( t=9.38, 5.06, 10.25 , P<0.05). There were statistical differences in lumbar vertebra, femoral neck, total hip BMD, lumbar vertebra SUVmen, total hip, lumbar SUVmax and indicators between group A and group B ( t=3.94, 9.21, 2.53, 3.95, 2.61 , P<0.05). Logistic regression analysis results showed that osteoporosis Exp[ (61.857 (3.029-1 263.408) , P<0.05], 25-OH-D[ (0.039 (0.005-0.312) , P<0.05], total hip BMD[ (480.040 (4.384-5 257.048) , P<0.05] were the main independent influencing factors causing VitD deficiency. Conclusions:There is a close relationship between 25-OH-D level, BMD expression and VitD deficiency in elderly patients with hip fractures. Among them, osteoporosis, 25-OH-D level, and total hip BMD are the main independent influencing factors that cause VitD deficiency.

Objective:To analyze the clinical features of elderly patients with hip fracture and the expression of serum vitamin D (VitD), 25- hydroxy vitamin D (25-OH-D) and bone mineral density,, BMD) .Methods:Collect clinical data from 90 elderly patients with hip fractures admitted to the Emergency Department of Fenyang Hospital in Shanxi Province from Oct. 2020 to Dec. 2023 for analysis. Based on whether the patients have VitD deficiency, they were divided into group A (severe deficiency of 25-OH-D, insufficient) and group B (sufficient 25-OH-D). Clinical characteristics of patients and the levels of VitD, 25-OH-D, and BMD, and the correlation between the above indicators were analyzed.Results:The age of patients in groups A and B, whether there is osteoporosis, and 25-OH-D levels are suspicious influencing factors that cause VitD deficiency ( t=9.38, 5.06, 10.25 , P<0.05). There were statistical differences in lumbar vertebra, femoral neck, total hip BMD, lumbar vertebra SUVmen, total hip, lumbar SUVmax and indicators between group A and group B ( t=3.94, 9.21, 2.53, 3.95, 2.61 , P<0.05). Logistic regression analysis results showed that osteoporosis Exp[ (61.857 (3.029-1 263.408) , P<0.05], 25-OH-D[ (0.039 (0.005-0.312) , P<0.05], total hip BMD[ (480.040 (4.384-5 257.048) , P<0.05] were the main independent influencing factors causing VitD deficiency. Conclusions:There is a close relationship between 25-OH-D level, BMD expression and VitD deficiency in elderly patients with hip fractures. Among them, osteoporosis, 25-OH-D level, and total hip BMD are the main independent influencing factors that cause VitD deficiency.

Objective To explore the value of CT signs and quantitative parameters of artificial intelligence (AI) in predicting the efficacy of neoadjuvant chemotherapy for colorectal cancer. Methods A total of 349 colorectal cancer patients who received neoadjuvant chemotherapy at Cangzhou Hospital of Integrated Traditional Chinese and Western Medicine in Hebei Province from January 2022 to January 2025 were selected and and divided into the effective group (n = 267) and the ineffective group (n = 82) according to the evaluation criteria for the efficacy of solid tumors. Conduct a CT examination and extract AI quantitative parameters from the CT images based on the lesion. The data were analyzed using SPSS21.0 software, Logistic regression was used to screen the influencing factors of ineffective neoadjuvant chemotherapy in patients with colorectal cancer, and separate and combined models of CT signs and AI quantitative parameters were established. The predictive effect of the model was verified by using the ROC curve, calibration curve and decision curve. Results Compared with the effective group, the proportion of regular tumor morphology and the proportion of non-enlarged lymph nodesin the ineffective group were smaller. The tumor volume, peak value and entropy value were larger (P < 0.05). Multivariable analysis showed that irregular shape (OR= 4.216), presence of lymph node enlargement (OR = 8.998), larger tumor volume (OR = 1.109), higher average CT value (OR = 1.120), elevated peak value (OR = 2.528), and increased entropy value (OR = 1.390) were independent risk factors for ineffective neoadjuvant chemotherapy in colorectal cancer (P < 0.05). The areas under the ROC curves of the individual and combined models of CT signs and AI quantitative parameters were 0.777, 0.818, and 0.877, respectively(P < 0.05). The calibration curve showed a Brier score of 0.091. The decision curve showed that the threshold was between 0.10 and 0.85, and the combined model achieved a relatively high net clinical benefit. Conclusion CT signs combined with AI quantitative parameters has a predictive value for the efficacy of neoadjuvant chemotherapy in colorectal cancer. To provide evidence-based basis for clinical screening of the population benefiting from chemotherapy and optimization of treatment strategies.

Objective:To investigate the effect of botulinum toxin type A on children with odorihidrosis.Methods:From March 2017 to February 2021, 121 children with odorihidrosis, including 48 males and 73 females, aged 13 to 17 (15.9±1.2) years, were admitted to the Burn and Plastic Surgery Department of the 980 Hospital of PLA. There were 24 cases in mild group, 50 cases in moderate group and 47 cases in severe group. Botulinum toxin A was injected into 20-50 points on each side, and 1 U was injected into each point. The total amount of botulinum toxin A was 50-100 U on both sides.Results:Three groups of children were evaluated for efficacy, 24 cases of mild group was significantly effective in 23 cases, accounting for 95.8%. In the moderate group, 46 (92.0%) of 50 cases showed obvious effect. 49 cases (98.0%) were effective; In the severe group, 40 cases (85.1%) showed obvious effect and 45 cases (95.7%) were effective. Three groups of children with different efficacy had no statistical significance ( P>0.05). The significant efficiency in mild and moderate groups was higher than that in severe group, and the difference was statistically significant ( P<0.05). Conclusions:Botulinum toxin type A is effective in the treatment of children with mild and moderate bromhidrosis, and is worthy of clinical application.

The gluteal fat grafting technique continues to grow in popularity, along with which the safety issue should not be ignored. It drew attentions of researchers that due to the existence of large vessels, fat embolism or fat embolism syndrome occurred more frequently for gluteal fat grafting, which might lead to death. In this situation, researchers performed a series of study including retrospective case series, systematic analysis, questionnaire survey and anatomical study, and summarized a series of safety principles for gluteal fat grafting, such as scanning high-risk patients; staying subcutaneously injection, avoiding intramuscular injection; using cannula size over 4 mm; keeping cannula parallel to gluteus maximus; injecting while withdrawing; and avoiding excessive local pressure. Based on the literature review, this article analyzes and summarizes the safety of gluteal fat grafting.

The gluteal fat grafting technique continues to grow in popularity, along with which the safety issue should not be ignored. It drew attentions of researchers that due to the existence of large vessels, fat embolism or fat embolism syndrome occurred more frequently for gluteal fat grafting, which might lead to death. In this situation, researchers performed a series of study including retrospective case series, systematic analysis, questionnaire survey and anatomical study, and summarized a series of safety principles for gluteal fat grafting, such as scanning high-risk patients; staying subcutaneously injection, avoiding intramuscular injection; using cannula size over 4 mm; keeping cannula parallel to gluteus maximus; injecting while withdrawing; and avoiding excessive local pressure. Based on the literature review, this article analyzes and summarizes the safety of gluteal fat grafting.

Background: Microsporum canis is a zoonotic disease that can cause dermatophytosis in animals and humans. Objectives: In clinical practice, ketoconazole (KTZ) and other imidazole drugs are commonly used to treat M. canis infection, but its molecular mechanism is not completely understood.The antifungal mechanism of KTZ needs to be studied in detail. Methods: In this study, one strain of fungi was isolated from a canine suffering with clinical dermatosis and confirmed as M. canis by morphological observation and sequencing analysis.The clinically isolated M. canis was treated with KTZ and transcriptome sequencing was performed to identify differentially expressed genes in M. canis exposed to KTZ compared with those unexposed thereto. Results: At half-inhibitory concentration (½MIC), compared with the control group, 453 genes were significantly up-regulated and 326 genes were significantly down-regulated (p < 0.05). Quantitative reverse transcription polymerase chain reaction analysis verified the transcriptome results of RNA sequencing. Gene ontology enrichment analysis and Kyoto Encyclopedia of Genes and Genomes enrichment analysis revealed that the 3 pathways of RNA polymerase, steroid biosynthesis, and ribosome biogenesis in eukaryotes are closely related to the antifungal mechanism of KTZ. Conclusions The results indicated that KTZ may change cell membrane permeability, destroy the cell wall, and inhibit mitosis and transcriptional regulation through CYP51, SQL, ERG6, ATM, ABCB1, SC, KER33, RPA1, and RNP genes in the 3 pathways. This study provides a new theoretical basis for the effective control of M. canis infection and the effect of KTZ on fungi.

Background The ongoing new coronavirus pneumonia (Corona Virus Disease 2019,COVID-19) outbreak is spreading in China, but it has not yet reached its peak. Five million people emigrated from Wuhan before lockdown, potentially representing a source of virus infection. Determining case distribution and its correlation with population emigration from Wuhan in the early stage of the epidemic is of great importance for early warning and for the prevention of future outbreaks. Methods The official case report on the COVID-19 epidemic was collected as of January 30, 2020. Time and location information on COVID-19 cases was extracted and analyzed using ArcGIS and WinBUGS software. Data on population migration from Wuhan City and Hubei province were extracted from Baidu Qianxi, and their correlation with the number of cases was analyzed. Results The COVID-19 confirmed and death cases in Hubei province accounted for 59.91% (5806/9692) and 95.77% (204/213) of the total cases in China respectively. Hot spot provinces included Sichuan and Yunnan, which are adjacent to Hubei. The time risk of Hubei province on the following day was 1.960 times that on the previous day. The number of cases in some cities was relatively low, but the time risk appeared to be continuously rising. The correlation coefficient between the provincial number of cases and emigration from Wuhan was up to 0.943. The lockdown of 17 cities in Hubei province and the implementation of nationwide control measures efficiently prevented an exponential growth in the number of cases. Conclusion The population that emigrated from Wuhan was the main infection source in other cities and provinces. Some cities with a low number of cases showed a rapid increase in case load. Owing to the upcoming Spring Festival return wave, understanding the risk trends in different regions is crucial to ensure preparedness at both the individual and organization levels and to prevent new outbreaks.