BACKGROUND:Magnetic nanomaterials,as a hot topic in the biomedical field in recent years,are often used to enhance the targeted delivery of drugs to the affected area.OBJECTIVE:To investigate the effects of magnetic nano drug carriers on skeletal muscle injury markers and inflammatory responses in rats with sports injuries.METHODS:Magnetic nanoparticles were prepared.A total of 88 male SD rats were randomly divided into a blank group(n=8),an injury control group(n=32),a Yunnan Baiyao group(n=24),and a magnetic nano-drug carrier group(n=24)by using a random number table method.The latter three groups were modeled with exercise-induced muscle injury(treadmill slope of-16°,running speed of 16 m/min,and training time of 120 min).Immediately after exercise,after verifying the success of the model,Yunnan Baiyao patch was applied to the gastrocnemius muscle of the rats in the Yunnan Baiyao group.Yunnan Baiyao patch loaded with magnetic nanoparticles was applied to the gastrocnemius muscle of the rats in the magnetic nano-drug carrier group.At 24,48,and 120 hours after exercise,blood was drawn from the abdominal aorta of rats to detect the activities of creatine kinase and lactate dehydrogenase,as well as the levels of myoglobin,interleukin-6,and tumor necrosis factor-α.Hematoxylin-eosin staining was used to observe the infiltration of inflammatory cells in the gastrocnemius muscle.RESULTS AND CONCLUSION:(1)Compared with the blank group,the levels of myoglobin,creatine kinase,lactate dehydrogenase and tumor necrosis factorα in the injury control group at 24,48 and 120 hours after exercise were increased(P＜0.05),and the level of interleukin 6 at 24 and 120 hours after exercise was increased(P＜0.05).Compared with the injury control group,the level of myoglobin in the Yunnan Baiyao group at 24 and 48 hours after exercise was decreased(P＜0.05),the activities of creatine kinase and lactate dehydrogenase at 24,48 and 120 hours were decreased(P＜0.05),and the levels of interleukin 6 and tumor necrosis factor α at 120 hours after exercise were decreased(P＜0.05).Compared with the Yunnan Baiyao group,the level of myoglobin in the magnetic nano-drug carrier group at 24 and 48 hours after exercise was decreased(P＜0.05),the activities of creatine kinase and tumor necrosis factor α at 48 and 120 hours after exercise were decreased(P＜0.05),and the lactate dehydrogenase activity was reduced(P＜0.05).(2)Hematoxylin-eosin staining showed that a large number of inflammatory cells infiltrated in the muscle fibers of the injury control group 24 hours after exercise,and then the inflammatory cell infiltration gradually decreased,and the local damaged muscle fibers began to regenerate 120 hours after exercise.A large number of inflammatory cells infiltrated in the muscle fibers of the Yunnan Baiyao group and the magnetic nano-drug carrier group 24 hours after exercise,and then the inflammatory cell infiltration gradually decreased,and the damaged muscle fibers were regenerating 120 hours after exercise,and there was no significant difference from the blank group.(3)The results show that Yunnan Baiyao patch combined with magnetic nanoparticles can accelerate the recovery of exercise-induced muscle injury in rats,and the effect is better than that of Yunnan Baiyao alone.

BACKGROUND:Magnetic nanomaterials,as a hot topic in the biomedical field in recent years,are often used to enhance the targeted delivery of drugs to the affected area.OBJECTIVE:To investigate the effects of magnetic nano drug carriers on skeletal muscle injury markers and inflammatory responses in rats with sports injuries.METHODS:Magnetic nanoparticles were prepared.A total of 88 male SD rats were randomly divided into a blank group(n=8),an injury control group(n=32),a Yunnan Baiyao group(n=24),and a magnetic nano-drug carrier group(n=24)by using a random number table method.The latter three groups were modeled with exercise-induced muscle injury(treadmill slope of-16°,running speed of 16 m/min,and training time of 120 min).Immediately after exercise,after verifying the success of the model,Yunnan Baiyao patch was applied to the gastrocnemius muscle of the rats in the Yunnan Baiyao group.Yunnan Baiyao patch loaded with magnetic nanoparticles was applied to the gastrocnemius muscle of the rats in the magnetic nano-drug carrier group.At 24,48,and 120 hours after exercise,blood was drawn from the abdominal aorta of rats to detect the activities of creatine kinase and lactate dehydrogenase,as well as the levels of myoglobin,interleukin-6,and tumor necrosis factor-α.Hematoxylin-eosin staining was used to observe the infiltration of inflammatory cells in the gastrocnemius muscle.RESULTS AND CONCLUSION:(1)Compared with the blank group,the levels of myoglobin,creatine kinase,lactate dehydrogenase and tumor necrosis factorα in the injury control group at 24,48 and 120 hours after exercise were increased(P＜0.05),and the level of interleukin 6 at 24 and 120 hours after exercise was increased(P＜0.05).Compared with the injury control group,the level of myoglobin in the Yunnan Baiyao group at 24 and 48 hours after exercise was decreased(P＜0.05),the activities of creatine kinase and lactate dehydrogenase at 24,48 and 120 hours were decreased(P＜0.05),and the levels of interleukin 6 and tumor necrosis factor α at 120 hours after exercise were decreased(P＜0.05).Compared with the Yunnan Baiyao group,the level of myoglobin in the magnetic nano-drug carrier group at 24 and 48 hours after exercise was decreased(P＜0.05),the activities of creatine kinase and tumor necrosis factor α at 48 and 120 hours after exercise were decreased(P＜0.05),and the lactate dehydrogenase activity was reduced(P＜0.05).(2)Hematoxylin-eosin staining showed that a large number of inflammatory cells infiltrated in the muscle fibers of the injury control group 24 hours after exercise,and then the inflammatory cell infiltration gradually decreased,and the local damaged muscle fibers began to regenerate 120 hours after exercise.A large number of inflammatory cells infiltrated in the muscle fibers of the Yunnan Baiyao group and the magnetic nano-drug carrier group 24 hours after exercise,and then the inflammatory cell infiltration gradually decreased,and the damaged muscle fibers were regenerating 120 hours after exercise,and there was no significant difference from the blank group.(3)The results show that Yunnan Baiyao patch combined with magnetic nanoparticles can accelerate the recovery of exercise-induced muscle injury in rats,and the effect is better than that of Yunnan Baiyao alone.

Objective To investigate the prognostic value of optic nerve sheath diameter(ONSD)measured by critical care ultrasound combined with serum biomarkers[S100 calcium-binding protein β(S100β)and neuron-specific enolase(NSE)]in patients with severe traumatic brain injury.Methods A total of 103 adult severe traumatic brain injury patients admitted to the intensive care unit of this hospital from A-pril 1,2023,to April 1,2024 were enrolled.All patients underwent invasive intracranial pressure monitoring after admission,alongside bedside critical care ultrasound measurement of ONSD at 3 mm behind the globe and serum biomarker testing.Baseline data and Glasgow outcome scale(GOS)scores at 90 days after dis-charge were recorded.Patients were divided into the survival and the non-survival groups based on GOS scores.Receiver operating characteristic(ROC)curve analysis and area under the curve(AUC)were used to evaluate the predictive performance of ONSD and serum biomarkers for poor prognosis in severe traumatic brain injury patients.Results Ninety-six patients were ultimately included,with 52(54.1％)in the survival group and 44(45.9％)in the non-survival group.Significant differences were observed in blood glucose,Glas-gow coma scale(GCS)score,acute physiology and chronic health evaluation Ⅱ(APACHE Ⅱ)score,ONSD,NSE,and S100β levels(P＜0.05)between the two groups.Multivariate analysis identified ONSD(OR=4.962,95％CI:3.473-6.254),NSE(OR=2.704,95％CI:1.987-3.033),S100β(OR=2.983,95％CI:1.843-4.571),and APACHE Ⅱ score(OR=3.726,95％CI:2.837-4.592)as independent predictors of mortality in severe traumatic brain injury patients(P＜0.05).The combination of ONSD,NSE,and S100β yielded an AUC of 0.840 for predicting poor prognosis,with a specificity of 88.3％and sensitivity of 98.6％.Conclusion ONSD and serum brain injury biomarkers(NSE,S100β)are associated with in-hospital prognosis in severe traumatic brain injury patients.Their combined detection can effectively predict a poor outcome.

Objective To propose a method for abdominal multi-organ segmentation assisted by multi-phase CT synthesis.Methods Multi-phase CT synthesis for synthesizing high-quality CT images was used to increase the information details for image segmentation.A transformer block was introduced to help to capture long-range semantic information in cooperation with perceptual loss to minimize the differences between the real image and synthesized image.Results The model was trained using multi-phase CT dataset of 526 total cases from Nanfang Hospital.The mean maximum absolute error(MAE)of the synthesized non-contrast CT,venous phase contrast-enhanced CT(CECT),and delay phase CECT images from arterial phase CECT was 19.192±3.381,20.140±2.676 and 22.538±2.874,respectively,which were better than those of images synthesized using other methods.Validation of the multi-phase CT synthesis-assisted abdominal multi-organ segmentation method showed an average dice coefficient of 0.847 for the internal validation set and 0.823 for the external validation set.Conclusion The propose method is capable of synthesizing high-quality multi-phase CT images to effectively reduce the errors in registration between different phase CT images and improve the performance for segmentation of 13 abdominal organs.

Objective To propose a method for abdominal multi-organ segmentation assisted by multi-phase CT synthesis.Methods Multi-phase CT synthesis for synthesizing high-quality CT images was used to increase the information details for image segmentation.A transformer block was introduced to help to capture long-range semantic information in cooperation with perceptual loss to minimize the differences between the real image and synthesized image.Results The model was trained using multi-phase CT dataset of 526 total cases from Nanfang Hospital.The mean maximum absolute error(MAE)of the synthesized non-contrast CT,venous phase contrast-enhanced CT(CECT),and delay phase CECT images from arterial phase CECT was 19.192±3.381,20.140±2.676 and 22.538±2.874,respectively,which were better than those of images synthesized using other methods.Validation of the multi-phase CT synthesis-assisted abdominal multi-organ segmentation method showed an average dice coefficient of 0.847 for the internal validation set and 0.823 for the external validation set.Conclusion The propose method is capable of synthesizing high-quality multi-phase CT images to effectively reduce the errors in registration between different phase CT images and improve the performance for segmentation of 13 abdominal organs.

The condition of patients with severe traumatic brain injury (sTBI) complicated by corona virus 2019 disease (COVID-19) is complex. sTBI can significantly increase the probability of COVID-19 developing into severe or critical stage, while COVID-19 can also increase the surgical risk of sTBI and the severity of postoperative lung lesions. There are many contradictions in the treatment process, which brings difficulties to the clinical treatment of such patients. Up to now, there are few clinical studies and therapeutic norms relevant to sTBI complicated by COVID-19. In order to standardize the clinical treatment of such patients, Critical Care Medicine Branch of China International Exchange and Promotive Association for Medical and Healthcare and Editorial Board of Chinese Journal of Trauma organized relevant experts to formulate the Chinese expert consensus on clinical treatment of adult patients with severe traumatic brain injury complicated by corona virus infection 2019 ( version 2023) based on the joint prevention and control mechanism scheme of the State Council and domestic and foreign literatures on sTBI and COVID-19 in the past 3 years of the international epidemic. Fifteen recommendations focused on emergency treatment, emergency surgery and comprehensive management were put forward to provide a guidance for the diagnosis and treatment of sTBI complicated by COVID-19.

Objective:To explore the relationship between the polymorphisms of SIRT1 and follicle-stimulating hormone receptor (FSHR) gene and ovarian function in women who underwent assisted reproductive technology (ART) and the differences between Mongolian and Han nationalities, so as to provide basis for the individualized treatment of ART assisted pregnancy. Methods:A retrospective cohort study was performed. The clinical data of 204 ART patients who visited the Reproductive Center of the Affiliated Hospital of Inner Mongolia Medical University from September 2019 to September 2021 were collected. There were 53 cases of Mongolian nationality and 151 cases of Han nationality. MassARRAY SNP genotyping technology was used to detect three sites, which were rs2236319 of SIRT1 and rs6165 and rs6166 of FSHR. SIRT1 was divided into three groups: AA group (wild type), AG group (heterozygous type) and GG group (mutant type); FSHR rs6165 was divided into TT group (wild type), AT group (heterozygous type), AA group (mutant type); FSHR rs6166 was divided into NN group (wild type), NS group (heterozygous type) and SS group (mutant type). The differences of genotype frequency and clinical and laboratory data were compared. Results:1) The allele frequencies and genotype frequencies of SIRT1 rs2236319, FSHR rs6165, and rs6166 loci were not statistically different in composition between Mongolian and Han ART female patient groups. There were no significant differences between Mongolian and Han ART women in terms of general clinical data and basal sex hormones and ovarian reserve function (all P>0.05). 2) There was a statistically significant difference in the number of eggs captured at the rs2236319 locus of the SIRT1 gene among the mutant GG group [18.5 (13.0, 32.8)], the AA group [13.0 (7.0, 20.0)] and the AG group [10.0 (5.0, 21.0), P=0.019]. 3) There was a statistically significant difference in the basal FSH levels at the rs6165 locus of the FSHR gene among the AA group [(7.45±2.73) U/L], the TT group [(5.97±2.23) U/L] and the TA group [(6.70±2.04) U/L, P=0.005]. The proportion of ovarian hyporesponsiveness among the AA group [29.6% (8/27)], the TT group [8.5% (8/94)] and the TA group [13.3% (11/83)] was statistically significant ( P=0.017). 4) The difference in basal FSH levels at the rs6166 locus of the FSHR gene among the SS group [(7.48±2.78) U/L], the NN group [(6.08±2.28) U/L] and the NS group [(6.61±1.99) U/L] was statistically significant ( P=0.016). The proportion of ovarian hyporesponsiveness among the SS group [37.0% (10/26)], the NN group [8.5% (8/99)] and the NS group [10.8% (9/79)] was statistically significant ( P=0.001). Conclusion:There were no significant differences between Mongolian and Han ART women in terms of general clinical data, basal sex hormones and ovarian reserve function. The gene polymorphism at rs2236319 of SIRT1 gene is associated with ovarian hyperresponsiveness. FSHR rs6165 and rs6166 gene polymorphisms are associated with ovarian hyporesponsiveness. There was no significant correlation between the three polymorphisms and the clinical pregnancy rate of ART.

Reproductive health is a basic element of human health and an important pillar of social sustainable development. Based on the 2030 sustainable development goal, a global perspective should be given to the broader relationship between human beings and the earth and the natural ecological environment. The purpose of reproductive health is not only to reduce the death rate and improve the quality and the rate of the birth, but also to promote the sustainable development of human beings, resources, and environment. This paper systematically discussed the development of reproductive health theory and the current situation of global reproductive health, in order to clarify the important status and role of reproductive health in the national health and to promote the high-quality development of maternal and child health.

Objective:To explore the effects of mouth opening breathing for different reasons on children's maxillofacial development. Methods:One hundred and fifty-one children were selected as the research objects of this experiment. They were divided into 49 cases of adenoid hypertrophy group（group A）, 52 cases of tonsillar hypertrophy group（group B） and 50 cases of adenoid with tonsillar hypertrophy group（Group C）. Healthy children in the same period were selected as the control group, a total of 45 cases. The reflex nasopharyngeal measurement parameters, facial development indexes and cephalometric parameters of group A, group B, group C and control group were analyzed, and the incidence of Angle ClassⅡand Angle Class Ⅲ in group A, group B and group C were studied. Results:Compared with the control group, the reflex nasopharyngeal measurement parameters in group A, group B and group C was significantly different（P<0.05）, and the cephalometric parameters changed with variation in groups（P<0.05）. The incidence of Angle Class Ⅱ facial pattern in group A and group C was higher, but the incidence of Angle Class Ⅲ facial pattern in group B and group C was higher（P<0.05）. Conclusion:Adenoid hypertrophy leads to mandibular retraction; tonsil hypertrophy leads to anterior mandibular arch; adenoid hypertrophy and tonsil hypertrophy are easy to lead to clockwise rotation of the mandible. In clinical practice, to avoid children's uncoordinated maxillofacial development, we should correct the maxillofacial situation of children as soon as possible.
Child ; Humans ; Maxillofacial Development ; Malocclusion, Angle Class III/complications* ; Nasopharynx ; Adenoids ; Palatine Tonsil ; Mouth Breathing/etiology* ; Hypertrophy/complications* ; Mouth

Objective:To explore the relationship between the polymorphisms of SIRT1 and follicle-stimulating hormone receptor (FSHR) gene and ovarian function in women who underwent assisted reproductive technology (ART) and the differences between Mongolian and Han nationalities, so as to provide basis for the individualized treatment of ART assisted pregnancy. Methods:A retrospective cohort study was performed. The clinical data of 204 ART patients who visited the Reproductive Center of the Affiliated Hospital of Inner Mongolia Medical University from September 2019 to September 2021 were collected. There were 53 cases of Mongolian nationality and 151 cases of Han nationality. MassARRAY SNP genotyping technology was used to detect three sites, which were rs2236319 of SIRT1 and rs6165 and rs6166 of FSHR. SIRT1 was divided into three groups: AA group (wild type), AG group (heterozygous type) and GG group (mutant type); FSHR rs6165 was divided into TT group (wild type), AT group (heterozygous type), AA group (mutant type); FSHR rs6166 was divided into NN group (wild type), NS group (heterozygous type) and SS group (mutant type). The differences of genotype frequency and clinical and laboratory data were compared. Results:1) The allele frequencies and genotype frequencies of SIRT1 rs2236319, FSHR rs6165, and rs6166 loci were not statistically different in composition between Mongolian and Han ART female patient groups. There were no significant differences between Mongolian and Han ART women in terms of general clinical data and basal sex hormones and ovarian reserve function (all P>0.05). 2) There was a statistically significant difference in the number of eggs captured at the rs2236319 locus of the SIRT1 gene among the mutant GG group [18.5 (13.0, 32.8)], the AA group [13.0 (7.0, 20.0)] and the AG group [10.0 (5.0, 21.0), P=0.019]. 3) There was a statistically significant difference in the basal FSH levels at the rs6165 locus of the FSHR gene among the AA group [(7.45±2.73) U/L], the TT group [(5.97±2.23) U/L] and the TA group [(6.70±2.04) U/L, P=0.005]. The proportion of ovarian hyporesponsiveness among the AA group [29.6% (8/27)], the TT group [8.5% (8/94)] and the TA group [13.3% (11/83)] was statistically significant ( P=0.017). 4) The difference in basal FSH levels at the rs6166 locus of the FSHR gene among the SS group [(7.48±2.78) U/L], the NN group [(6.08±2.28) U/L] and the NS group [(6.61±1.99) U/L] was statistically significant ( P=0.016). The proportion of ovarian hyporesponsiveness among the SS group [37.0% (10/26)], the NN group [8.5% (8/99)] and the NS group [10.8% (9/79)] was statistically significant ( P=0.001). Conclusion:There were no significant differences between Mongolian and Han ART women in terms of general clinical data, basal sex hormones and ovarian reserve function. The gene polymorphism at rs2236319 of SIRT1 gene is associated with ovarian hyperresponsiveness. FSHR rs6165 and rs6166 gene polymorphisms are associated with ovarian hyporesponsiveness. There was no significant correlation between the three polymorphisms and the clinical pregnancy rate of ART.