Objective:To reduce associated risks in the field of laboratory information through risk management software.Method:A risk control team was established to conduct an application research on risk management. Based on ISO 15189 Clause 7.6 control of data and information management, the risk control team used the software to conduct risk assessments on the First Affiliated Hospital, Zhejiang University School of Medicine in 2024 through methods such as brainstorming, Failure Mode and Effects Analysis (FMEA). Risk control, risk monitoring, and risk management review were implemented.Result:42 risk points were identified in the fields of data control and information management. After evaluation by the risk control team,it was determined that one item is high-risk with a risk number of 51.56 and three items are medium risk with risk numbers of 35.45, 31.27, and 30.75 The risk priority numbers of the 4 risk points were reduced to acceptable levels below 25 after taking appropriate risk control measures, then the 4 risk points were monitored.Conclusion:The application of risk management software is helpful for the implementation of risk management in the laboratory information field.

Objective:To reduce associated risks in the field of laboratory information through risk management software.Method:A risk control team was established to conduct an application research on risk management. Based on ISO 15189 Clause 7.6 control of data and information management, the risk control team used the software to conduct risk assessments on the First Affiliated Hospital, Zhejiang University School of Medicine in 2024 through methods such as brainstorming, Failure Mode and Effects Analysis (FMEA). Risk control, risk monitoring, and risk management review were implemented.Result:42 risk points were identified in the fields of data control and information management. After evaluation by the risk control team,it was determined that one item is high-risk with a risk number of 51.56 and three items are medium risk with risk numbers of 35.45, 31.27, and 30.75 The risk priority numbers of the 4 risk points were reduced to acceptable levels below 25 after taking appropriate risk control measures, then the 4 risk points were monitored.Conclusion:The application of risk management software is helpful for the implementation of risk management in the laboratory information field.

OBJECTIVETo observe the changes of surface ECG and cell couplings between sinoatrial node cells and myocardial cells following transplantion of pedicled autologous sinoatrial node tissue graft into the right ventricle of a canine model of complete atrioventricular block.

METHODSTen healthy dogs were randomized into transplantation group and control group. Pedicled autologous sinoatrial node tissue grafts were transplanted into the right ventricle in the transplantation group, while the sinoatrial nodes were only excised in the control group after placement of temporary myocardial pacing wires. The changes of surface ECG were observed at 1, 2, 3 and 4 weeks postoperatively. At 4 weeks, complete atrioventricular block was induced in the dogs by radiofrequency ablation of the His bundle. The heart rate of the dogs in both groups were recorded after the injection of isoproternol (ISO) from the femoral vein, and the transplanted tissue graft was observed under optical and transmission electron microscopes.

RESULTSNo significant changes occurred in the surface ECG. All the dogs showed ECG waveforms specific of complete heart block after the ablation, and the ventricular heart rates were similar between the two groups (P>0.05). The ventricular heart rate did not undergo obvious changes after ISO injection (P>0.05). The transplanted pedicled autologous sinoatrial node survived in the dogs and the sinoatrial node cells established desmosome junctions with the myocardial cells, but the number of junctions was not sufficient to support heart pacing.

CONCLUSIONDesmosome junction can occur between ventricular myocardial cells and sinoatrial node cells at the edge of transplanted pedicled autologous sinoatrial node tissue.

Animals ; Atrioventricular Block ; physiopathology ; surgery ; Cardiotonic Agents ; pharmacology ; Dogs ; Electrocardiography ; Female ; Heart Rate ; drug effects ; Heart Ventricles ; surgery ; Intercellular Junctions ; Isoproterenol ; pharmacology ; Male ; Myocardium ; cytology ; Sinoatrial Node ; cytology ; transplantation ; Tissue Transplantation ; Transplantation, Autologous

Objective To observe the changes of surface ECG and cell couplings between sinoatrial node cells and myocardial cells following transplantion of pedicled autologous sinoatrial node tissue graft into the right ventricle of a canine model of complete atrioventricular block. Methods Ten healthy dogs were randomized into transplantation group and control group. Pedicled autologous sinoatrial node tissue grafts were transplanted into the right ventricle in the transplantation group, while the sinoatrial nodes were only excised in the control group after placement of temporary myocardial pacing wires. The changes of surface ECG were observed at 1, 2, 3 and 4 weeks postoperatively. At 4 weeks, complete atrioventricular block was induced in the dogs by radiofrequency ablation of the His bundle. The heart rate of the dogs in both groups were recorded after the injection of isoproternol (ISO) from the femoral vein, and the transplanted tissue graft was observed under optical and transmission electron microscopes. Results No significant changes occurred in the surface ECG. All the dogs showed ECG waveforms specific of complete heart block after the ablation, and the ventricular heart rates were similar between the two groups (P>0.05). The ventricular heart rate did not undergo obvious changes after ISO injection (P>0.05). The transplanted pedicled autologous sinoatrial node survived in the dogs and the sinoatrial node cells established desmosome junctions with the myocardial cells, but the number of junctions was not sufficient to support heart pacing. Conclusions Desmosome junction can occur between ventricular myocardial cells and sinoatrial node cells at the edge of transplanted pedicled autologous sinoatrial node tissue.

Objective To observe the changes of surface ECG and cell couplings between sinoatrial node cells and myocardial cells following transplantion of pedicled autologous sinoatrial node tissue graft into the right ventricle of a canine model of complete atrioventricular block. Methods Ten healthy dogs were randomized into transplantation group and control group. Pedicled autologous sinoatrial node tissue grafts were transplanted into the right ventricle in the transplantation group, while the sinoatrial nodes were only excised in the control group after placement of temporary myocardial pacing wires. The changes of surface ECG were observed at 1, 2, 3 and 4 weeks postoperatively. At 4 weeks, complete atrioventricular block was induced in the dogs by radiofrequency ablation of the His bundle. The heart rate of the dogs in both groups were recorded after the injection of isoproternol (ISO) from the femoral vein, and the transplanted tissue graft was observed under optical and transmission electron microscopes. Results No significant changes occurred in the surface ECG. All the dogs showed ECG waveforms specific of complete heart block after the ablation, and the ventricular heart rates were similar between the two groups (P>0.05). The ventricular heart rate did not undergo obvious changes after ISO injection (P>0.05). The transplanted pedicled autologous sinoatrial node survived in the dogs and the sinoatrial node cells established desmosome junctions with the myocardial cells, but the number of junctions was not sufficient to support heart pacing. Conclusions Desmosome junction can occur between ventricular myocardial cells and sinoatrial node cells at the edge of transplanted pedicled autologous sinoatrial node tissue.