OBJECTIVE: This study aims to elucidate the therapeutic potential of osthole for the treatment of atopic dermatitis (AD), focusing on its ability to alleviate chronic pruritus (CP) and the underlying molecular mechanisms. METHODS: In this study, we investigated the anti-inflammatory effects of osthole in both a 2,4-dichloronitrobenzene (DNCB)-induced AD mouse model and tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ) stimulated huma immortalized epidermal (HaCaT) cells. The anti-itch effect of osthole was specifically assessed in the AD mouse model. Using methods such as hematoxylin and eosin (HE) staining, enzyme-linked immunosorbent assay (ELISA), western blot (WB), quantitative real-time PCR (qRT-PCR), and immunofluorescence staining. RESULTS: Osthole improved skin damage and clinical dermatitis scores, reduced scratching bouts, and decreased epidermal thickness AD-like mice. It also reduced the levels of interleukin (IL)-31 and IL-31 receptor A (IL-31 RA) in both skin tissues and HaCaT cells. Furthermore, Osthole suppressed the protein expression levels of phosphor-p65 (p-p65) and phosphor-inhibitor of nuclear factor kappa-Bα (p-IκBα). Meanwhile, it increased the protein expression levels of peroxisome proliferator-activated receptor α (PPARα) and PPARγ in HaCaT cells. CONCLUSION These findings indicated that osthole effectively inhibited CP in AD by activating PPARα, PPARγ, repressing the NF-κB signaling pathway, as well as the expression of IL-31 and IL-31 RA.

Objective:To investigate the current situation of kinesiophobia in patients with in vitro fertilization and embryo transfer and to analyse its influencing factors, so as to provide a reference for the intervention research on kinesiophobia in patients with embryo transfer.Methods:Patients in the first trimester of pregnancy after in vitro fertilization and embryo transfer in the Women's Hospital, School of Medicine, Zhejiang University from August to October 2024 were selected by convenience sampling method, and the general information questionnaire, Tampa Scale of Kine-siophobia (TSK), General Perceived Self-Efficacy Scale (GSES), the Brief Illness Perception Questionnaire (BIPQ) and Social Support Rating Sca1e (SSRS) were used as research tools for cross-sectional investigation, and the influencing factors were analysed by univariate and multiple linear regression.Results:A total of 318 pregnant women in the early stage were finally included, with an average age of (33.11 ± 4.55) years old. The TSK score was (40.40 ± 4.22) points, the GSES score was (25.64 ± 5.25) points, the BIPQ score was (34.30 ± 9.97) points, the SSRS score was (37.98 ± 5.65) points, among which 79.25%(252/318) cases had kinesiophobia. Multiple linear regression analysis showed that age, educational level, exercise frequency before transplantation, self-efficacy, and disease perception were important influencing factors of kinesiophobia in the early pregnancy of in vitro fertilization-embryo transfer patients ( t values were -3.39 to 3.11, all P<0.05). Conclusions:The incidence of kinesiophobia in embryo transfer patients is at a medium to high level, and nursing staff should pay attention to patients with young age, low education level and low daily exercise, and at the same time improve patients′self-efficacy and control of disease perception, reduce the occurrence of kinesitophobia in early pregnancy, improve mental state during pregnancy and promote their health.

Objective:To investigate the current situation of kinesiophobia in patients with in vitro fertilization and embryo transfer and to analyse its influencing factors, so as to provide a reference for the intervention research on kinesiophobia in patients with embryo transfer.Methods:Patients in the first trimester of pregnancy after in vitro fertilization and embryo transfer in the Women's Hospital, School of Medicine, Zhejiang University from August to October 2024 were selected by convenience sampling method, and the general information questionnaire, Tampa Scale of Kine-siophobia (TSK), General Perceived Self-Efficacy Scale (GSES), the Brief Illness Perception Questionnaire (BIPQ) and Social Support Rating Sca1e (SSRS) were used as research tools for cross-sectional investigation, and the influencing factors were analysed by univariate and multiple linear regression.Results:A total of 318 pregnant women in the early stage were finally included, with an average age of (33.11 ± 4.55) years old. The TSK score was (40.40 ± 4.22) points, the GSES score was (25.64 ± 5.25) points, the BIPQ score was (34.30 ± 9.97) points, the SSRS score was (37.98 ± 5.65) points, among which 79.25%(252/318) cases had kinesiophobia. Multiple linear regression analysis showed that age, educational level, exercise frequency before transplantation, self-efficacy, and disease perception were important influencing factors of kinesiophobia in the early pregnancy of in vitro fertilization-embryo transfer patients ( t values were -3.39 to 3.11, all P<0.05). Conclusions:The incidence of kinesiophobia in embryo transfer patients is at a medium to high level, and nursing staff should pay attention to patients with young age, low education level and low daily exercise, and at the same time improve patients′self-efficacy and control of disease perception, reduce the occurrence of kinesitophobia in early pregnancy, improve mental state during pregnancy and promote their health.

Objective To construct an evaluation index system of core competence of assisted reproductive technology specialist nurses,and to conduct preliminary validation,so as to provide reference standards for the training and evaluation of assisted reproductive technology specialist nurses.Methods Based on the core competence of specialist nurses of the International Council of Nurses and the Hamric Advanced Practice Nurse competence framework as the theoretical framework,through literature research and research group discussion,the core competence index of specialist nurses in assisted reproductive technology was preliminarily established.A total of 23 experts were consulted with 2 rounds of Delphi method to complete index and determine weight coefficients in April 2024.In August 2024,50 assisted reproductive technology specialist nurses from 18 provinces and cities across the country were selected to conduct a satisfaction survey on the evaluation index system,in order to preliminarily verify the application effect of indicators.Results The questionnaire recovery rates for 2 rounds of expert consultations were both 100％,and the expert authority coefficients were both 0.943.The coefficient of variation was 0～0.287 and 0～0.236,and the Kendall harmony coefficient was 0.241～0.337 and 0.250～0.431,respectively.The final evaluation index of core competence of assisted reproductive technology specialist nurses included 6 first-level indicators,20 second-level indicators and 57 third-level indicators.Assisted reproductive technology specialist nurses were satisfied with the evaluation index system of core competence,with a score of(18.26±2.52).Conclusion The evaluation index system of core competence of assisted reproductive technology specialist nurses is practical,with comprehensive content and outstanding professional characteristics.

Objective To construct an evaluation index system of core competence of assisted reproductive technology specialist nurses,and to conduct preliminary validation,so as to provide reference standards for the training and evaluation of assisted reproductive technology specialist nurses.Methods Based on the core competence of specialist nurses of the International Council of Nurses and the Hamric Advanced Practice Nurse competence framework as the theoretical framework,through literature research and research group discussion,the core competence index of specialist nurses in assisted reproductive technology was preliminarily established.A total of 23 experts were consulted with 2 rounds of Delphi method to complete index and determine weight coefficients in April 2024.In August 2024,50 assisted reproductive technology specialist nurses from 18 provinces and cities across the country were selected to conduct a satisfaction survey on the evaluation index system,in order to preliminarily verify the application effect of indicators.Results The questionnaire recovery rates for 2 rounds of expert consultations were both 100％,and the expert authority coefficients were both 0.943.The coefficient of variation was 0～0.287 and 0～0.236,and the Kendall harmony coefficient was 0.241～0.337 and 0.250～0.431,respectively.The final evaluation index of core competence of assisted reproductive technology specialist nurses included 6 first-level indicators,20 second-level indicators and 57 third-level indicators.Assisted reproductive technology specialist nurses were satisfied with the evaluation index system of core competence,with a score of(18.26±2.52).Conclusion The evaluation index system of core competence of assisted reproductive technology specialist nurses is practical,with comprehensive content and outstanding professional characteristics.

BACKGROUND: Currently the hematopoietic stem cells can be obtained from bone marrow, peripheral blood and cord blood, so it is expected to search a new source of stem cells in order to satisfy the clinical transplantation needs. From the 5th week of pregnancy, the blood sinusoid system develops completely in liver, and then hematopoietic stem cells can move with blood flow. OBJECTIVE: To observe the biological features of human fetal blood hematopoietic stem/progenitor cells (HS/PCs), and their transplantation into non-obese diabetic/severe combined immunodeficiency disease (NOD/ SCID) mice. DESIGN: Control trial. SETTING: Department of Hematology, First Affiliated Hospital of Guangxi Medical University. MATERIALS:①Cell resource: Twenty-one fetal blood samples were from dead fetus [gestational age of 18-29 weeks, mean (24.2±3.2) weeks] and twenty-one full-term cord blood samples were provided from the Department of Obstetrics, First Affiliated Hospital of Guangxi Medical University between October 2002 and February 2003, with the consent of their relatives.②Experimental animal: Twelve NOD/SCID female mice of 6-7 weeks old were bred in sterility and super-clean operation board. METHODS: Flow cytometer was used to assess cell surface markers of HS/PCs including CD34, CD38, HLA-DR and CD90 in 21 human fetal blood samples, and their expressions were compared with 21 human cord blood samples. Moreover, human fetal blood mononuclear cells (MNCs) were transplanted into 6 NOD/SCID mice irradiated sublethally. After 5 weeks, human leukocytic content was also detected in bone marrow of mice with flow cytometer while human Cart-1 gene in recipients' bone marrow was sensed with polymerase chain reaction (PCR).MAIN OUTCOME MEASURES: ① Expressions of HS/PCs surface markers in fetal blood and cord blood. ②Implantation of fetal blood cells into NOD/SCID mice.RESULTS: ①The percentage of CD34+ cells in fetal blood was significantly higher than that of full-term cord blood [(2.258 8±0.720 9)%,(1.572 9±0.478 3)%, P=0.000 4]. The percentages of CD34+CD38- cells and CD34+CD90+ cells in fetal blood were also higher than those of fullterm cord blood [(1.298 6±0.470 6)%, (0.871 0±0.409 5)%, P=0.001 6;(0.930 0±0.469 2)%, (0.560 0±0.365 8)%, P=0.032 4].②Four cases (4/6)of human fetal blood MNCs smoothly transplanted the hematopoiesis of sublethally irradiated NOD/SCID mice. Five weeks after the transplantation, human leukocyte and Cart-1 gene could still be detected in marrow cells of NOD/SCID mice.CONCLUSION: Human fetal blood contains more HS/PCs than cord blood. Human fetal blood MNCs can engraft bone marrow of NOD/SCID mice and reconstitute general hemopoiesis of marrow and lymph systems.Human fetal blood is a new possible source of pluripotential hematopoietic stem cell.

In this paper the method of TQC was used to analyze various factors that effect drug quality in hospital pharmacy. In order to raise clinic safety and use drugs reasonably and effectively, the corresponding tactics were taken for the related problems that influence drug quality. Thus, scientific management of drugs, improvement of preparation quality, decrease of consume, and increasing income and sparing expenses were attained.