Objective To observe the clinical efficacy of acupuncture combined with Fire Dragon Cupping Therapy in the treatment of chronic non-specific low back pain(CNLBP)based on the bowstring mechanics theory.Methods A total of 80 patients diagnosed with CNLBP and admitted to the Acupuncture Department of Shenzhen Pingle Orthopedic and Traumatology Hospital(Shenzhen Pingshan District Hospital of Traditional Chinese Medicine)from January 2024 to December 2024 were selected as the study subjects.The patients were randomly divided into an observation group and a control group using a random number table,with 40 cases in each group.The control group received conventional acupuncture treatment,while the observation group received Fire Dragon Cupping Therapy in addition to the same acupuncture treatment as the control group.The treatment lasted for 2 weeks,with a follow-up conducted 3 months after treatment.After 2 weeks of treatment,the clinical efficacy of both groups was evaluated.Changes in the Visual Analogue Scale(VAS)score,modified Oswestry Disability Index(ODI)score,serum interleukin 6(IL-6)and tumor necrosis factor α(TNF-α)levels were observed before and after treatment.Additionally,changes in lumbar flexion,extension,lateral flexion,and rotation range of motion(ROM),as well as endurance time of lumbar flexor and extensor muscles,were compared between the two groups.Results(1)The total effective rate was 97.50％(39/40)in the observation group and 82.50％(33/40)in the control group.The efficacy of the observation group was superior to that of the control group,the difference being statistically significant(P＜0.05).(2)After treatment and at the 3-month follow-up,both groups showed significant improvements in VAS and ODI scores(P＜0.05),with the observation group demonstrating significantly better improvements than the control group,the differences being statistically significant(P＜0.05).(3)After treatment and at the 3-month follow-up,serum IL-6 and TNF-α levels significantly decreased in both groups(P＜0.05),with the observation group showing significantly greater reductions than the control group,the differences being statistically significant(P＜0.05).(4)After treatment and at the 3-month follow-up,lumbar flexion,extension,lateral flexion,and rotation ROM significantly improved in both groups(P＜0.05),with the observation group exhibiting significantly better improvements than the control group,the difference being statistically significant(P＜0.05).(5)After treatment and at the 3-month follow-up,lumbar flexor and extensor muscle endurance time significantly improved in both groups(P＜0.05),with the observation group demonstrating significantly better improvements than the control group,the difference being statistically significant(P＜0.05).Conclusion The combination of acupuncture and Fire Dragon Cupping Therapy based on the bowstring mechanics theory can significantly alleviate pain,restore lumbar function,and promote functional recovery in patients with CNLBP,demonstrating notable clinical efficacy.

Objective To analyze the detection rate of carbapenem-resistant Klebsiella pneumoniae(CRKP)in Guangdong Provincial Second Hospital of Traditional Chinese Medicine(the hospital)and analyze the main drug resistance genes and virulence genes of CRKP,so as to understand the molecular epidemiologi-cal mechanism of its infection strains.Methods The detection rate of CRKP infection in the hospital from 2020 to 2023 was retrospectively analyzed,and a total of 84 strains of CRKP were collected from July to De-cember 2022 in the hospital.The clinical data of the strains were collected,and the corresponding drug resist-ance genes and virulence genes were amplified by PCR.The modified carbapenem inactivation method(mCIM)was uesd to detect carbapenemase.Results The detection rates of CRKP in the Guangdong Provincial Second Hospital of Traditional Chinese Medicine from 2020 to 2023 were relatively high,and were higher than 46.00％.84 non-repeated CRKP strains were collected from July to December of 2022,and most samples were from respiratory tract,accounting for 55.95％.The patients were mainly from acupuncture and rehabilitation departments,accounting for 34.52％.Drug sensitivity tests showed that CRKP was highly resistant to various cephalosporin and extended spectrum beta lactam drugs,and only showed high sensitivity to tigecycline and polymyxin.The positive rate of mCIM test was 84.52％(71/84),and the other 15.48％results were neutral,which failed to determine whether they produced carbapenemase.A total of 73 strains were detected with car-bapenemase gene,accounting for 86.90％,involving 4 genotypes.The detection rates of blaKPC,blaNDM,blaIMP,and blaOXA-48 were 83.33％,2.38％,1.19％,and 1.19％,respectively.One of them carried both blaKPC and blaNDM genes,and multiple β-lactamases were detected.The detection rates of blaSHV,bla-TEM,blaCTX-M-9,and blaCTX-M-1 were 96.43％,78.57％,64.29％,and 2.38％,respectively.The detection rates of five virulence genes,blaiucA,blarmpA2,blairoB,blapeg-334,and blarmpA,were 42.86％,41.67％,27.38％,3.57％,and 2.38％,respectively.The proportion of strains carrying three or more virulence genes was 17.85％(15/84).Conclusion The detection rate of CRKP in the hospital is relatively high,and the drug resistance situation is serious,with Klebsiella pneumoniae carbapenemase(KPC)as the main drug resistance gene.A high proportion of Carbapenem-resistant hypervirulent Klebsiella pneumoniae(CR-hvKp)strains is found,and the infection prevention and control situation is not optimistic.It is necessary to further strengthen the hospital infection control measures and standardize the scientific and reasonable drug use.

Objective To evaluate the effect of computer-aided design of composite materials with epoxide acrylate maleic (E) and hydroxyapatite (H) in cranioplasty. Methods A total of 45 patients with cranium defects were treated with cranioplasty by using skull bone flaps made of composite materials including epoxide acrylate maleic (E) and hydroxyapatite (H) ,which was designed with computer aid according to individual requirements. The patients were followed up for 6-36 months. Results After cranioplasty with composite EH, there occurred subcutaneous fluid in one patient and mild bone collapse in one. The composite EH showed good histocompatibility, with no infection or rejection. Conclusion During cranioplasty, use of computer-aided design of composite EH takes advantages of good accuracy, short operation time, good biocompatibility and good clinical efficiency.