This study analyzes the clinical data of a patient presenting with acute poisoning following the co-ingestion of a pesticide (approximately 100-120 ml of 41% glyphosate-isopropylammonium) and alcohol (approximately 100 g of ethanol). The patient developed multiple organ dysfunction syndrome (MODS), manifesting as acute kidney injury (AKI), metabolic acidosis, hyperkalemia, acute lung injury (ALI), acute liver injury, acute intestinal injury, and systemic inflammatory response syndrome (SIRS). Following administration of general supportive care combined with continuous renal replacement therapy (CRRT) and hemoperfusion (HP), the patient recovered fully and was discharged. Acute combined glyphosate and ethanol poisoning can induce MODS. Analysis of this patient's diagnosis and treatment process enhances clinicians' understanding of this toxicological presentation and offers valuable clinical insights to improve survival rates in similar cases.

This study analyzes the clinical data of a patient presenting with acute poisoning following the co-ingestion of a pesticide (approximately 100-120 ml of 41% glyphosate-isopropylammonium) and alcohol (approximately 100 g of ethanol). The patient developed multiple organ dysfunction syndrome (MODS), manifesting as acute kidney injury (AKI), metabolic acidosis, hyperkalemia, acute lung injury (ALI), acute liver injury, acute intestinal injury, and systemic inflammatory response syndrome (SIRS). Following administration of general supportive care combined with continuous renal replacement therapy (CRRT) and hemoperfusion (HP), the patient recovered fully and was discharged. Acute combined glyphosate and ethanol poisoning can induce MODS. Analysis of this patient's diagnosis and treatment process enhances clinicians' understanding of this toxicological presentation and offers valuable clinical insights to improve survival rates in similar cases.

Objective To explore whether remote ischemic preconditioning(RIPC)can prolong the time of ultra-intense exercise(110%VO2max)by increasing the maximum cumulative oxygen deficit(MA-OD),and the specific ways of energy supply of the anaerobic metabolic system.Methods Twenty-four racquet athletes(22.2±2.0 years;174±9 cm;67.1±12.4 kg)completed three supramaximal intensi-ty tests on a treadmill at 110%VO2max intensity to exhaustion separated with Control,Placebo and RIPC interventions.RIPC was induced on the limbs on both sides(5×5 min alternating bilateral occlu-sion 220 and 60 mmHg for Placebo and RIPC interventions,respectively).Moreover,all groups under-went a fourth test with incremental load,and a fifth test with constant load at 40%,50%,60%,70%and 80%VO2max.Results The time to exhaustion and the MAOD of the RIPC group were both greater than those in the Placebo and Control groups(P<0.05).However,no significant differences were found in the average alternative maximal accumulated oxygen deficit(MAODALT),lactic anaerobic metabolism,alactic anaerobic metabolism and parameters of excess post-exercise oxygen consump-tion dynamic curve of the three groups(P>0.05).Meanwhile,in the RIPC group,the average MAOD was significantly higher than MAODALT(P<0.05).Pearson correlation analysis showed a significant rela-tionship between the improvement of MAOD and an increase in exhaustion time after RIPC interven-tion.Conclusion RIPC can improve supramaximal exercise performance of racquet athletes by enhanc-ing their MAOD,and the enhancement of glycolysis energy supply and lactic acid elimination is a po-tential intermediary of the improvement of sports performance.

Protein-RNA interaction networks are essential to understand gene regulation control. Identifying binding sites of RNA-binding proteins (RBPs) by the UV-crosslinking and immunoprecipitation (CLIP) represents one of the most powerful methods to map protein-RNA interactions in vivo. However, the traditional CLIP protocol is technically challenging, which requires radioactive labeling and suffers from material loss during PAGE-membrane transfer procedures. Here we introduce a super-efficient CLIP method (GoldCLIP) that omits all gel purification steps. This nonisotopic method allows us to perform highly reproducible CLIP experiments with polypyrimidine tract-binding protein (PTB), a classical RBP in human cell lines. In principle, our method guarantees sequencing library constructions, providing the protein of interest can be successfully crosslinked to RNAs in living cells. GoldCLIP is readily applicable to diverse proteins to uncover their endogenous RNA targets.
Binding Sites ; Cell Line ; Heterogeneous-Nuclear Ribonucleoproteins ; isolation & purification ; metabolism ; Humans ; Immunoprecipitation ; methods ; Polypyrimidine Tract-Binding Protein ; isolation & purification ; metabolism ; RNA ; isolation & purification ; metabolism ; RNA-Binding Proteins ; isolation & purification ; metabolism

Russia's post-degree medical education first appeared in the end of the 19th century and had improved along with the progress in science, technology and education. From 1990s, new waves of technology revolution and the rise of post-occupation training further pushed the post-degree medical education to move forward. Legally established post-degree education as a form of occupational education enables Russian medical professionals to constantly update knowledge and absorb new information to keep up with the new developments in medical science and technology. In this sense, post-degree medical education has become a major way to train not only medical professionals but professionals of acupuncture and moxibustion, and thus has been of great significance for TCM and acupuncture to transmit and develop in Soviet Union and Russia. This paper introduced the general situation of post-degree medical education in Russia, its influence to the transmission and development of TCM and acupuncture in Soviet Union and Russia, as well as the enlightenment to medical education.