Establishing a culture system throughout the whole cycle of medical education is the key task in cultivating medical talents in the new era.This study adopts a case study approach to examine the"Four-Level,Four-Dimension"cultural education model implemented at Xiangya Medical College.By analyzing the four-level collaborative pathway of"school-hospital-department-individual"and combining with the four dimensions of"culture guidance,practice integration,specialty collaboration and individual development".This paper discusses the role and effectiveness of this model in cultivating medical humanistic liter-acy and inheriting medical spirit and provides"Xiangya experience"for the construction of medical education culture system in the new era.

Objective To investigate the current status of adherence to home ultrafiltration volume measurement in peri-toneal dialysis(PD)patients and explore its influencing factors,providing a basis for optimizing volume management strategies.Methods A cross-sectional study design was adopted.In March 2024,a questionnaire survey was conducted among 324 regular-ly followed-up patients at the Peritoneal Dialysis Center of the First Affiliated Hospital of Xi'an Jiaotong University.The question-naire covered demographic characteristics,ultrafiltration volume recording behaviors,measurement method awareness,and instru-ment preferences.Possible factors influencing adherence to ultrafiltration volume measurement were analyzed.Results The o-verall compliance rate for recording patients' ultrafiltration volume was 56.2％(182/324)％.The completion rate of measure-ment was better in the measuring cup group compared to the weighing method(57.3％vs 47.4％,P=0.034).However,Meas-urement compliance decreased with prolonged dialysis duration(P=0.021).78.39％of patients could identify emergency situa-tions of volume expansion(e.g.,sudden decrease in ultrafiltration volume accompanied by elevated blood pressure).However,patients with lower education levels(below high school)had poorer awareness of volume changes compared to those with higher education(81.4％vs 18.5％,x2=9.946,P=0.041).The recognition rate for volume depletion was significantly lower than that for volume expansion(x2=7.163,P=0.008).Multivariate logistic regression analysis showed that higher education level(OR=0.404,95％CI 0.228-0.717,P=0.002),family assistance in measurement(OR=0.361,95％CI 0.183-0.713,P=0.003),and strong belief that measurement helps doctors adjust prescriptions(OR=0.371,95％CI 0.187-0.736,P=0.005)were protective factors for compliance with ultrafiltration volume measurement in peritoneal dialysis patients.Longer dialysis dura-tion(OR=4.732,95％CI 2.315-9.674,P＜0.001)and perception of measurement as troublesome(OR=4.802,95％CI 2.787-8.272,P＜0.001)were independent risk factors for poor compliance with ultrafiltration measurement.Compared with pa-tients with low educational level,patients with high educational level had higher requirements for the ease of operation(38.6％vs 61.4％,x2=15.033,P＜0.001),cost performance(41.1％vs 58.9％,x2=13.335,P＜0.001),and durability(38.2％vs 61.8％,x2=4.558,P=0.033)of the new ultrafiltration measurement equipment.Conclusion ① Long dialysis duration,per-ceived measurement inconvenience,low education level,self-measurement,and insufficient trust in physicians are independent risk factors for poor adherence to home ultrafiltration volume measurement among PD patients.②The vast majority of patients can recognize emergency situations involving sudden volume overload,but their awareness and recognition of volume depletion require further improvement.③New measuring instruments,such as intelligent measuring cups,with easy-to-use operations can be pro-moted.When combined with remote monitoring technology,they help patients identify capacity insufficiency promptly and im-prove the rate of early intervention.

Establishing a culture system throughout the whole cycle of medical education is the key task in cultivating medical talents in the new era.This study adopts a case study approach to examine the"Four-Level,Four-Dimension"cultural education model implemented at Xiangya Medical College.By analyzing the four-level collaborative pathway of"school-hospital-department-individual"and combining with the four dimensions of"culture guidance,practice integration,specialty collaboration and individual development".This paper discusses the role and effectiveness of this model in cultivating medical humanistic liter-acy and inheriting medical spirit and provides"Xiangya experience"for the construction of medical education culture system in the new era.

Objective To investigate the current status of adherence to home ultrafiltration volume measurement in peri-toneal dialysis(PD)patients and explore its influencing factors,providing a basis for optimizing volume management strategies.Methods A cross-sectional study design was adopted.In March 2024,a questionnaire survey was conducted among 324 regular-ly followed-up patients at the Peritoneal Dialysis Center of the First Affiliated Hospital of Xi'an Jiaotong University.The question-naire covered demographic characteristics,ultrafiltration volume recording behaviors,measurement method awareness,and instru-ment preferences.Possible factors influencing adherence to ultrafiltration volume measurement were analyzed.Results The o-verall compliance rate for recording patients' ultrafiltration volume was 56.2％(182/324)％.The completion rate of measure-ment was better in the measuring cup group compared to the weighing method(57.3％vs 47.4％,P=0.034).However,Meas-urement compliance decreased with prolonged dialysis duration(P=0.021).78.39％of patients could identify emergency situa-tions of volume expansion(e.g.,sudden decrease in ultrafiltration volume accompanied by elevated blood pressure).However,patients with lower education levels(below high school)had poorer awareness of volume changes compared to those with higher education(81.4％vs 18.5％,x2=9.946,P=0.041).The recognition rate for volume depletion was significantly lower than that for volume expansion(x2=7.163,P=0.008).Multivariate logistic regression analysis showed that higher education level(OR=0.404,95％CI 0.228-0.717,P=0.002),family assistance in measurement(OR=0.361,95％CI 0.183-0.713,P=0.003),and strong belief that measurement helps doctors adjust prescriptions(OR=0.371,95％CI 0.187-0.736,P=0.005)were protective factors for compliance with ultrafiltration volume measurement in peritoneal dialysis patients.Longer dialysis dura-tion(OR=4.732,95％CI 2.315-9.674,P＜0.001)and perception of measurement as troublesome(OR=4.802,95％CI 2.787-8.272,P＜0.001)were independent risk factors for poor compliance with ultrafiltration measurement.Compared with pa-tients with low educational level,patients with high educational level had higher requirements for the ease of operation(38.6％vs 61.4％,x2=15.033,P＜0.001),cost performance(41.1％vs 58.9％,x2=13.335,P＜0.001),and durability(38.2％vs 61.8％,x2=4.558,P=0.033)of the new ultrafiltration measurement equipment.Conclusion ① Long dialysis duration,per-ceived measurement inconvenience,low education level,self-measurement,and insufficient trust in physicians are independent risk factors for poor adherence to home ultrafiltration volume measurement among PD patients.②The vast majority of patients can recognize emergency situations involving sudden volume overload,but their awareness and recognition of volume depletion require further improvement.③New measuring instruments,such as intelligent measuring cups,with easy-to-use operations can be pro-moted.When combined with remote monitoring technology,they help patients identify capacity insufficiency promptly and im-prove the rate of early intervention.

Objective To construct an injectable hydrogel(IH)entrapment system of bone marrow mesenchymal stem cells(BM-MSCs)and to explore its effect of promoting articular cartilage repair and underlying mechanism.Methods The primary BM-MSCs of mice were cultured by whole bone marrow adherent method.The differentiation potential was identified by alizarin red(ARS),oil red O(ORO)and Alcian blue(AB)staining,and the expres-sion of CD44,CD90,CD105,CD34 and CD45 on the surface was detected by flow cytometry.BM-MSCs loaded with IH(BM-MSC-IH)was prepared,and the rheological properties of BM-MSC-IH were tested by rheological test.The model of full-thickness injury of knee cartilage in mice was established,and the mice were randomly divided into normal saline group,BM-MSC group and BM-MSC-IH group with 8 in each.The mice were injected with BM-MSC-IH 0.2 mL,cell suspension 0.2 mL and 0.9％NaCl solidion,independently.After 6 and 12 weeks of in-jection,the repair of knee cartilage in mice was observed by safranin-fast green staining,and the expression of typeⅡ collagen in knee joint was examined by immunohistochemical staining microscopy.Results The primary cultured cells showed positive expression of CD44,CD90 and CD105,but negative expression of CD34 and CD45,and had the potential of osteogenic,adipogenic and chondrogenic differentiation.The constructed BM-MSC-IH sys-tem was solid colloid,and the rheological properties were consistent with the basic characteristics of hydrogel.BM-MSC-IH effectively promoted the regeneration of mouse knee cartilage and increase the local expression of typeⅡ collagen.Conclusions Bone marrow-mesenchymal stem cells embedded with injectable hydrogel effectively pro-mote the repair of injured cartilage and the underlying mechanism is potentially promoting the differentiation of chondrocytes and the expression of type Ⅱ collagen.Its effect is better than application of BM-MSCs alone.

Infertility is a global health issue,and more and more people are hoping to have babies by means of assisted reproductive technology.However,there are still many challenges in fertilization and pregnancy outcomes.Sperm quality is a key factor affecting the success rate of assisted reproduction.Therefore,sperm quality screening is crucial for achieving breakthroughs in assisted reproduction technology.At present,with its capabilities in the field of image recognition,artificial intelligence(AI)is providing new ideas and methods for sperm screening.Various attempts have been made with AI-based models to evaluate indicators such as sperm morphology,DNA quality,and motility level,and some results have been achieved.Herein,we reviewed the application of AI in sperm quality analysis and selection,providing support for the future development of AI and the improvement in the fertilization rate and outcomes of assisted reproductive technology.

【Objective】 To explore the recruitment and retention strategy of blood donors by investigating the age composition of blood donors in some areas of China, so as to promote blood donation and enhance clinical blood supply. 【Methods】 Through the working platform of Practice Comparison Working Group of China’s Mainland Blood Collection and Supply Institutions, the average age and age composition of blood donors from 22 blood centers were collected, and statistical analysis was conducted after eliminating invalid data. 【Results】 The median average age of blood donors during the survey year was 30.02.The median age in 2.89% of the blood centers was lower than 25. The average age of different genders was statistically significant only in 2018(P<0.05). Fot first-time blood donors, the median constituent ratio of donors <25 and ≥25 years old was 54.53% and 44.28%, with median retention rate at 10.30% and 9.61%, respectively. The median overall participation rate of blood donors was 2.7%, with median participation rate of blood donors <25 years old at 5.1%. 【Conclusion】 The recruitment and retention of blood donor is crucial to enhance clinical blood supply. Blood donors <25 years old, with a longer period for future donation, should be the main target of blood donation recruitment. Meanwhile, the revision of upper age limit for blood donation is another important initiative to grow the blood donor pool.

【Objective】 To provide reference for fine management of blood donors by classifying and analyzing different types of blood donors from domestic blood stations. 【Methods】 The resident population of 15 regions in China from 2016 to 2019 were taken as the research object, among which the blood donors were divided into three categories: age-eligible citizens, registered donors and donated donors. The average value and proportion of the three categories were calculated and statistically analyzed. 【Results】 The resident population of the 15 regions varied greatly. The mean 95% CI of the proportion of age-eligible citizens to resident population from 2016 to 2019 was (60.16%, 67.84%); registered donors to age-eligible citizens and resident population was (2.21%, 2.86%) and (1.41%, 1.79%), respectively; donated donors to registered donors, age-eligible citizens and resident population was (84.63%, 91.68%), (1.93%, 2.55%) and(1.23%, 1.59%), respectively. 【Conclusion】 There were differences in the number and proportion of different types of blood donors in different regions. The fine management of blood donors can help blood stations carry out more effective recruitment and retention strategies.

The oral cavity of each person is home to hundreds of bacterial species.While taxa for oral diseases have been studied using culture-based characterization as well as amplicon sequencing,metagenomic and genomic information remains scarce compared to the fecal microbiome.Here,using metagenomic shotgun data for 3346 oral metagenomic samples together with 808 published samples,we obtain 56,213 metagenome-assembled genomes(MAGs),and more than 64％of the 3589 species-level genome bins(SGBs)contain no publicly available genomes.The resulting genome collection is representative of samples around the world and contains many genomes from candi-date phyla radiation(CPR)that lack monoculture.Also,it enables the discovery of new taxa such as a genus Candidatus Bgiplasma within the family Acholeplasmataceae.Large-scale metagenomic data from massive samples also allow the assembly of strains from important oral taxa such as Por-phyromonas and Neisseria.The oral microbes encode genes that could potentially metabolize drugs.Apart from these findings,a strongly male-enriched Campylobacter species was identified.Oral sam-ples would be more user-friendly collected than fecal samples and have the potential for disease diagnosis.Thus,these data lay down a genomic framework for future inquiries of the human oral microbiome.

Objective:To investigate the correlations between perfused lung volumes, visual scores (using perfusion SPECT/CT) and right-heart catheter (RHC) hemodynamic parameters in patients with chronic thromboembolic pulmonary hypertension (CTEPH).Methods:A total of 51 consecutive CTEPH patients (17 males, 34 females, age (59±12) years) in the First Affiliated Hospital of Guangzhou Medical University between March 2015 and July 2019 were retrospectively analyzed. All patients underwent lung perfusion SPECT/CT imaging and RHC examinations. Perfused lung volumes were determined using threshold-based (15%-85%) segmentation. Visual semiquantitative scoring in each lung segment was performed using Begic method. RHC hemodynamic parameters including pulmonary artery systolic pressure (PASP), pulmonary arterial diastolic pressure (PADP), mean pulmonary artery pressure (mPAP), pulmonary arteriolar wedge pressure (PAWP), pulmonary vessel resistance (PVR), cardiac output (CO), cardiac index (CI) were recorded. Spearman correlation analysis was used to evaluate the correlations between perfused lung volumes, visual scores and hemodynamic parameters.Results:There were significant correlations between perfused lung volumes (30%-70% threshold) and mPAP ( rs values: from -0.414 to -0.302, all P<0.05). Among them, perfused lung volumes under the threshold of 40% and 45% were moderately correlated with mPAP ( rs values: -0.414, -0.412, both P<0.05). Perfused lung volume (40% threshold) was moderately negatively correlated with PASP, PADP ( rs values: -0.402, -0.440, both P<0.05), and slightly negatively correlated with PVR ( rs=-0.352, P<0.05). Visual scores were slightly positively correlated with the PADP ( rs=0.311, P<0.05), while there was no correlation between visual scores and other RHC hemodynamic parameters ( rs values: from -0.201 to 0.275, all P>0.05). Conclusion:Perfused lung volumes based on threshold-based segmentation in lung perfusion SPECT/CT imaging can accurately reflect hemodynamic status and may provide useful information for severity assessment of CTEPH.