ObjectiveTo observe the effect of Shenshu Fujian decoction on platelet-derived growth factor （PDGF）/naked cuticle homolog 2 （NKD2） /Wnt signaling pathway in rats with chronic renal failure （CRF）. MethodsSixty male SD rats were randomly divided into normal group， model group， Niaoduqing group （5 g·kg^-1）， low-dose Shenshu Fujian decoction group （5.5 g·kg^-1）， medium-dose Shenshu Fujian decoction group （11 g·kg^-1）， and high-dose Shenshu Fujian decoction group （22 g·kg^-1）， with 10 rats in each group. A CRF rat model was established by feeding a 0.5% adenine diet for 21 days. After successful modeling， intragastric administration was given once daily for 28 consecutive days. After treatment， the renal morphology of rats was observed. Serum creatinine （SCr） and blood urea nitrogen （BUN） levels were detected. Hematoxylin-eosin （HE） staining and Masson staining were used to detect renal histopathological changes， and collagen volume fraction （CVF） was calculated. Serum levels of inflammatory markers interleukin （IL）-1β and IL-6 were measured using enzyme-linked immunosorbent assay （ELISA）. The expressions of fibronectin 1 （FN1）， type Ⅰ collagen （ColⅠ）， α-smooth muscle actin （α-SMA）， platelet-derived growth factor receptor-β （PDGFR-β）， NKD2， dishevelled protein 2 （DVL2） and β-catenin in renal tissue were detected by immunohistochemistry and Western blot. ResultsCompared with the normal group， the model group showed significant renal pathological changes， a markedly increased kidney weight/body weight ratio （P<0.01）， significantly elevated CVF （P<0.01）， and notably increased serum levels of SCr， BUN， IL-1β， and IL-6 （P<0.01）. Expression levels of FN1， ColⅠ， α-SMA， PDGFR-β， NKD2， DVL2， and β-catenin in renal tissue were also significantly increased （P<0.01）. Compared with the model group， all treatment groups showed significantly decreased kidney weight/body weight ratios and CVF （P<0.01）， as well as markedly decreased serum SCr， BUN， IL-1β， and IL-6 levels. Protein expression levels of FN1， ColⅠ， α-SMA， PDGFR-β， NKD2， DVL2， and β-catenin in renal tissue were decreased， with more pronounced effects observed in the Niaoduqing， medium-dose， and high-dose Shenshu Fujian decoction groups （P<0.05， P<0.01）. ConclusionShenshu Fujian decoction improves renal function， reduces inflammation， and reverses renal fibrosis in CRF rats， possibly by downregulating the expression of PDGF/NKD2/Wnt signaling pathway-related proteins.

Objective To understand the status and influencing factors of kinesiophobia in patients with diabetic peripheral neuropathic pain(DPNP).Methods From February to June 2023,192 patients with DPNP in a tertiary general hospital in Nanchang City,Jiangxi Province were selected by convenience sampling method.The patients were investigated by the demographic information questionnaire,Tampa Scale for Kinesiophobia,Numerical Rating Scale,Medical Coping Modes Questionnaire,Diabetes Distress Scale,and Summary of Diabetes Self-care Activities.Multiple linear regression were used to analyze the influencing factors of kinesiophobia in patients with DPNP.Results The score of kinesiophobia in patients with DPNP was(36.68±3.88),and the incidence of kinesiophobia was 38.5％.Multiple linear regression analysis showed that marital status,pain level,coping style,diabetic distress and self-management behavior were influential factors for kinesiophobia in patients with DPNP(all P＜0.05).Conclusion Patients with DPNP have a moderate level of kinesiophobia.Nursing staff should pay attention to the early assessment of patients'kinesiophobia,promptly identify and take effective measures to reduce the level of patients'kinesiophobia,increase their motivation to exercise,and improve their prognosis and quality of life.

Objective To evaluate the application value of production-oriented approach (POA) teaching combined with individualized teaching in the training of scientific research liaison officers in surgical nursing. Methods A total of 58 scientific research liaison officers from 21 departments in the department of surgery at Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences),Southern Medical University were selected as the study subjects from January 2022 to December 2023. They received training based on POA teaching combined with individualized teaching. Specific methods included:① Establishing a surgical nursing research team consisting of 1 lead supervisor,2 secretaries,and department head nurses as mentors. Nurses with a master's degree in surgery and relevant experience were appointed as assistant mentors. One scientific research liaison officer was selected to participate in literature sharing and assist with departmental research tasks under the guidance of the district head nurse.② Developing a teaching plan:First,a variety of methods were used to assess the current research capabilities of the research liaison officers. The liaison team then designed a "semi-structured interview outline" to survey the training needs and suggestions of the liaison officers. Finally,the mentor dynamically adjusts the training objectives,training content,training time,training methods,etc. based on the survey results,training objectives,and learners' learning progress. ③ Collaborative teaching:the POA teaching method,consisting of 3 stages,motivation,facilitation,and evaluation,was combined with individualized teaching to develop a new model for training the research capabilities of nursing staff. A survey designed by hospital research mentors was used to assess the satisfaction,research capabilities,and research project application rates of the research liaison officers before and after the training. Results Compared to the training before the intervention,the post-training satisfaction with the course content,methods,progress,time arrangement,feedback and adjustment,as well as the scores in research theory,research practice,mentor evaluation,self-evaluation,peer evaluation,and total research capacity were significantly improved[satisfaction score:course content was 87.28±6.81 vs. 78.46±6.32,methods was 91.45±6.60 vs. 77.12±5.74,progress was 89.46±5.31 vs. 75.47±5.83,time arrangement was 86.08±6.52 vs. 72.56±7.43,feedback and adjustment was 83.49±7.03 vs. 73.72±6.21;research capacity scores:research theory was 17.87±1.62 vs. 13.45±3.68,research practice was 16.43±2.35 vs. 11.57±2.14,mentor evaluation was 16.78±2.19 vs. 12.35±2.45,self-evaluation was 17.84±1.35 vs. 11.21±2.30,peer evaluation was 17.02±2.26 vs. 12.59±3.45,and total score was 85.94±6.37 vs. 61.17±5.28,all P<0.05]. Additionally,the paper output rate,in-hospital project application rate,fund application rate,and patent application rate all significantly increased[paper output rate:53.45％ (31/58) vs. 27.59％ (16/58);in-hospital project application rate:28.57％ (6/21) vs. 4.76％ (1/21);fund application rate:33.33％ (7/21) vs. 4.76％ (1/21);patent application rate:38.10％ (8/21) vs. 9.52％ (2/21),all P<0.05]. Conclusion The application of POA teaching combined with individualized teaching in the training of scientific research liaison officers in surgical nursing can enhance the research capabilities of nursing staff and increase research output.

Objective To evaluate the application value of production-oriented approach (POA) teaching combined with individualized teaching in the training of scientific research liaison officers in surgical nursing. Methods A total of 58 scientific research liaison officers from 21 departments in the department of surgery at Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences),Southern Medical University were selected as the study subjects from January 2022 to December 2023. They received training based on POA teaching combined with individualized teaching. Specific methods included:① Establishing a surgical nursing research team consisting of 1 lead supervisor,2 secretaries,and department head nurses as mentors. Nurses with a master's degree in surgery and relevant experience were appointed as assistant mentors. One scientific research liaison officer was selected to participate in literature sharing and assist with departmental research tasks under the guidance of the district head nurse.② Developing a teaching plan:First,a variety of methods were used to assess the current research capabilities of the research liaison officers. The liaison team then designed a "semi-structured interview outline" to survey the training needs and suggestions of the liaison officers. Finally,the mentor dynamically adjusts the training objectives,training content,training time,training methods,etc. based on the survey results,training objectives,and learners' learning progress. ③ Collaborative teaching:the POA teaching method,consisting of 3 stages,motivation,facilitation,and evaluation,was combined with individualized teaching to develop a new model for training the research capabilities of nursing staff. A survey designed by hospital research mentors was used to assess the satisfaction,research capabilities,and research project application rates of the research liaison officers before and after the training. Results Compared to the training before the intervention,the post-training satisfaction with the course content,methods,progress,time arrangement,feedback and adjustment,as well as the scores in research theory,research practice,mentor evaluation,self-evaluation,peer evaluation,and total research capacity were significantly improved[satisfaction score:course content was 87.28±6.81 vs. 78.46±6.32,methods was 91.45±6.60 vs. 77.12±5.74,progress was 89.46±5.31 vs. 75.47±5.83,time arrangement was 86.08±6.52 vs. 72.56±7.43,feedback and adjustment was 83.49±7.03 vs. 73.72±6.21;research capacity scores:research theory was 17.87±1.62 vs. 13.45±3.68,research practice was 16.43±2.35 vs. 11.57±2.14,mentor evaluation was 16.78±2.19 vs. 12.35±2.45,self-evaluation was 17.84±1.35 vs. 11.21±2.30,peer evaluation was 17.02±2.26 vs. 12.59±3.45,and total score was 85.94±6.37 vs. 61.17±5.28,all P<0.05]. Additionally,the paper output rate,in-hospital project application rate,fund application rate,and patent application rate all significantly increased[paper output rate:53.45％ (31/58) vs. 27.59％ (16/58);in-hospital project application rate:28.57％ (6/21) vs. 4.76％ (1/21);fund application rate:33.33％ (7/21) vs. 4.76％ (1/21);patent application rate:38.10％ (8/21) vs. 9.52％ (2/21),all P<0.05]. Conclusion The application of POA teaching combined with individualized teaching in the training of scientific research liaison officers in surgical nursing can enhance the research capabilities of nursing staff and increase research output.

【Objective】 To explore the feasibility of using autoregressive moving average model (ARIMA) to predict the dosage of suspended red blood cells in children, and to provide a basis for the development of clinical blood reserve plans in children's hospitals. 【Methods】 ARIMA model was constructed using the total blood consumption of clinical suspended red blood cells from March 2016 to May 2022 at the Children's Hospital of Chongqing Medical University as the data source by SPSS26.0 software. The optimal model was used to predict the clinical suspended red blood cell consumption from June to October 2022, and the predictive effect of the model was tested. 【Results】 ARIMA(0, 1, 1) (0, 1, 1)12 was the optimal model for predicting the consumption of suspended red blood cells in pediatrics. The autocorrelation function and partial autocorrelation function of the residual sequence basically fell within the 95% confidence interval. At the same time, Ljung-Box Q statistical results showed that there was no correlation between the residual (P>0.05), indicating that the residual was white noise, which met the randomicity hypothesis. The average relative error between the predicted values of the model and the actual clinical red blood cell usage from June to October 2022 was 5%, indicating high prediction accuracy. 【Conclusion】 The blood usage of children has obvious seasonal and periodic patterns, and the optimal model ARIMA (0, 1, 1) (0, 1, 1)12 can better fit the trend of changes in pediatric suspended red blood cell usage, thus providing a basis for the development of clinical blood reserve plans in children's hospitals.

Objective:To explore the clinical value of preconception expanded carrier screening (PECS) in Chinese Han population of childbearing age.Methods:The gene detection results of infertile couples with PECS in the Reproductive Medicine Center of the Second Affiliated Hospital of Zhengzhou University from September 2019 to May 2022 were analyzed retrospectively. The carrier rate of pathogenic gene, the detection rate of high-risk couples and the clinical outcome of high-risk couples were counted and analyzed.Results:A total of 1 565 patients received PECS and they were all Chinese Han. A total of 504 patients received the 108 extended monogenic diseases testing, including 420 females and 84 males, the overall carrier rate of the target genes was 30.75% (129/420), and the detection rate of high-risk couples was 1.19% (1/84), the higher carrier rates of the tested genes were MMACHC [2.58% (13/504)], ATP7B [2.38% (12/504)], SLC22A5 [2.18% (11/504)], GALC [1.79% (9/504)], PAH [1.79% (9/504)] and MLC1 [1.19% (6/504)], the rest are less than 1%. There were 555 patients accepted FMR1 gene detection, and 5 patients with FMR1 gene mutation, accounting for 0.90%. Testing for direct relatives of patients with complete mutations, her mother is a pre mutation carrier with a CGG repeat count of 105. A total of 502 patients accepted SMN1 gene testing. Totally 14 femals and 2 males were found to be SMN1 gene carriers in this study, with a carrier rate of 3.19%. Conclusion:The carryier rate of single gene recessive disorder is high in the population. Screening before pregnancy can provide birth health guidance for patients, help them to choose preimplantation genetic testing for monogenic/single gene disorders (PGT-M) and prenatal diagnosis, to avoid the birth of silk children.

Objective:To explore the clinical value of preconception expanded carrier screening (PECS) in Chinese Han population of childbearing age.Methods:The gene detection results of infertile couples with PECS in the Reproductive Medicine Center of the Second Affiliated Hospital of Zhengzhou University from September 2019 to May 2022 were analyzed retrospectively. The carrier rate of pathogenic gene, the detection rate of high-risk couples and the clinical outcome of high-risk couples were counted and analyzed.Results:A total of 1 565 patients received PECS and they were all Chinese Han. A total of 504 patients received the 108 extended monogenic diseases testing, including 420 females and 84 males, the overall carrier rate of the target genes was 30.75% (129/420), and the detection rate of high-risk couples was 1.19% (1/84), the higher carrier rates of the tested genes were MMACHC [2.58% (13/504)], ATP7B [2.38% (12/504)], SLC22A5 [2.18% (11/504)], GALC [1.79% (9/504)], PAH [1.79% (9/504)] and MLC1 [1.19% (6/504)], the rest are less than 1%. There were 555 patients accepted FMR1 gene detection, and 5 patients with FMR1 gene mutation, accounting for 0.90%. Testing for direct relatives of patients with complete mutations, her mother is a pre mutation carrier with a CGG repeat count of 105. A total of 502 patients accepted SMN1 gene testing. Totally 14 femals and 2 males were found to be SMN1 gene carriers in this study, with a carrier rate of 3.19%. Conclusion:The carryier rate of single gene recessive disorder is high in the population. Screening before pregnancy can provide birth health guidance for patients, help them to choose preimplantation genetic testing for monogenic/single gene disorders (PGT-M) and prenatal diagnosis, to avoid the birth of silk children.

Objective:To establish a method for quantitative analysis of the active ingredients including salidroside, rosarin and rosavin and content determination in Rhodiola rosea at different harvest months. Methods:HPLC was used on an X selectHSS T3 (250 mm×4.6 mm, 5 μm) column with mobile phase consisting of methol-acetonitrile-phosphoric acid (0.05%) aqueous solution for gradient elution at a flow rate of 1 ml/min. The wavelength was detected at 275 nm (salidroside) and 254 nm (rosarin, rosavin). The column temperature was set at 30 ℃ and the injection volume was 5 μl.Results:The peak areas of Salidroside, rosarin and rosavin showed good linear relationships ( r > 0.999) with the content in the ranges of 44-1 420, 10-307 and 18-573 μg, respectively. The method was precise, stable, repeatable and the sample recovery test all well satisfied the requirements of quantitative analysis. The highest accumulation of the active ingredients was observed in Rhodiola rosea in September and the content of salidroside, rosarin and rosavin were 0.66, 0.07 and 0.53 mg/g, respectively. Conclusion:This method is simple and rapid to evaluate the content of active ingredients in Rhodiola rosea.

Objective:To explore the characteristics and nursing measures of medical rescue support in space station.Methods:According to the characteristics of medical rescue support task of manned space mission, practical nursing schemes and corresponding nursing measures were formulated from the aspects of personnel selection, personnel training, material preparation, process formulation, training exercise and epidemic prevention.Results:The "Shenzhou-12" mission includes the whole launch phase, operation phase and return phase. There are many tasks that cover a long time. The contents of medical rescue support include personnel's physical and mental quality, technical ability, environmental equipment, the possible injuries of astronauts, and the normalization of COVID-19's task guarantee. In view of this content, a practical nursing scheme was formulated to enable nurses to quickly master and skillfully cooperate with the treatment, win more time for the treatment and improve the overall rescue level.Conclusions:Various process plans have been integrated and optimized for medical rescue support of space mission. The equipment carried is advanced, and can be used in various complex environments and complex working conditions. The rescue support personnel have also done their best, kept improving and solidified the state. We should constantly sum up experience, explore and study new problems, and make new achievements in medical rescue support for space missions.

To analyze how the handover were effected by the conditions of manned spaceflight medical support mission through the practice of medical equipment and drugs in Shenzhou-12 and Shenzhou-13 manned spaceflight medical rescue support missions, this article discussed the preparation, organization and implementation in the handover of medical equipment and drugs in the changing of medical rescue teams, summarized the notices in the work of handover, and provided experience for the smooth handover of different manned spaceflight medical rescue teams in the future.