Objective:To explore the effectiveness of the "three-stage, four-point, and three-whole" curriculum ideological and political teaching design in the curriculum ideological and political education of surgical nursing.Methods:Using the whole group sampling method, 148 students from three classes of high vocational nursing in the grade of 2021 in Guangdong Food and Drug Vocational College were selected as control group, and the traditional curriculum ideological and political teaching was applied. The 175 students in four classes of high vocational nursing in the grade of 2022 were selected as experimental group, and the "three-stage, four-point, and three-whole" curriculum ideological and political teaching design program was applied. At the end of the course, the final theoretical assessment scores of the two groups of students and their evaluation of the overall curriculum ideological and political teaching were compared. A self-designed questionnaire was used to investigate the satisfaction of the students in experimental group with the penetration of the 16 ideological and political elements.Results:The final theoretical assessment scores of the students in experimental and control groups were (81.79±8.61) and (79.45±10.20) , respectively, and the difference was statistically significant ( P<0.05) . The evaluation scores of the students in experimental and control groups on the overall curriculum ideological and political teaching were (21.83±1.32) and (21.00±1.48) , respectively, and the differences were statistically significant ( P<0.05) . Students in experimental group rated > 4 points on their satisfaction with the penetration of the 15 ideological and political elements. Conclusions:Surgical nursing "three-stage, four-point, three-whole" ideological and political teaching design improves students' academic performance and satisfaction with curriculum ideological and political teaching in vocational colleges.

Objective:To explore the effectiveness of the "three-stage, four-point, and three-whole" curriculum ideological and political teaching design in the curriculum ideological and political education of surgical nursing.Methods:Using the whole group sampling method, 148 students from three classes of high vocational nursing in the grade of 2021 in Guangdong Food and Drug Vocational College were selected as control group, and the traditional curriculum ideological and political teaching was applied. The 175 students in four classes of high vocational nursing in the grade of 2022 were selected as experimental group, and the "three-stage, four-point, and three-whole" curriculum ideological and political teaching design program was applied. At the end of the course, the final theoretical assessment scores of the two groups of students and their evaluation of the overall curriculum ideological and political teaching were compared. A self-designed questionnaire was used to investigate the satisfaction of the students in experimental group with the penetration of the 16 ideological and political elements.Results:The final theoretical assessment scores of the students in experimental and control groups were (81.79±8.61) and (79.45±10.20) , respectively, and the difference was statistically significant ( P<0.05) . The evaluation scores of the students in experimental and control groups on the overall curriculum ideological and political teaching were (21.83±1.32) and (21.00±1.48) , respectively, and the differences were statistically significant ( P<0.05) . Students in experimental group rated > 4 points on their satisfaction with the penetration of the 15 ideological and political elements. Conclusions:Surgical nursing "three-stage, four-point, three-whole" ideological and political teaching design improves students' academic performance and satisfaction with curriculum ideological and political teaching in vocational colleges.

Objective:To investigate the mechanism by which curcumin activates the PINK1/Parkin pathway to enhance mitophagy and ameliorate endothelial cell damage induced by oxygen-glucose deprivation/reperfusion(OGD/R).Methods:An OGD/R injury model was established in mouse brain microvascular endothelial cells(BMECs).En-dothelial cells were treated with different concentrations of curcumin.The effects of curcumin on mitophagy in BMECs were assessed using immunofluorescence and transmission electron microscopy.Cell proliferation,injury,and barrier integrity were evaluated using a Cell Counting Kit-8(CCK-8),lactate dehydrogenase(LDH)assay,and transendothe-lial electrical resistance(TEER)measurement,respectively.Western Blot was used to detect the effects of curcumin on the expression of PINK1/Parkin pathway proteins(LC-3 Ⅱ/Ⅰ,PINK1,Parkin and p62).Additionally,cells were co-treated with Mdivi-1 to further confirm the role of mitophagy in curcumin's protective effects against OGD/R injury.Results:Curcumin treatment significantly promoted mitophagy,increased the expression of LC-3 Ⅱ/Ⅰ,PINK1,and Par-kin proteins(P＜0.001),and decreased the expression of p62 protein(P＜0.001).In the OGD/R model,curcumin significantly enhanced cell proliferation rates(P＜0.001),reduced LDH release(P＜0.001),and improved TEER values(P＜0.001).However,these protective effects of curcumin were reversed by the mitophagy inhibitor Mdivi-1.Conclusion:Curcumin effectively alleviates cell damage in BMECs under OGD/R conditions by enhancing mitophagy through the activation of the PINK1/Parkin pathway.

Objective:To investigate the mechanism by which curcumin activates the PINK1/Parkin pathway to enhance mitophagy and ameliorate endothelial cell damage induced by oxygen-glucose deprivation/reperfusion(OGD/R).Methods:An OGD/R injury model was established in mouse brain microvascular endothelial cells(BMECs).En-dothelial cells were treated with different concentrations of curcumin.The effects of curcumin on mitophagy in BMECs were assessed using immunofluorescence and transmission electron microscopy.Cell proliferation,injury,and barrier integrity were evaluated using a Cell Counting Kit-8(CCK-8),lactate dehydrogenase(LDH)assay,and transendothe-lial electrical resistance(TEER)measurement,respectively.Western Blot was used to detect the effects of curcumin on the expression of PINK1/Parkin pathway proteins(LC-3 Ⅱ/Ⅰ,PINK1,Parkin and p62).Additionally,cells were co-treated with Mdivi-1 to further confirm the role of mitophagy in curcumin's protective effects against OGD/R injury.Results:Curcumin treatment significantly promoted mitophagy,increased the expression of LC-3 Ⅱ/Ⅰ,PINK1,and Par-kin proteins(P＜0.001),and decreased the expression of p62 protein(P＜0.001).In the OGD/R model,curcumin significantly enhanced cell proliferation rates(P＜0.001),reduced LDH release(P＜0.001),and improved TEER values(P＜0.001).However,these protective effects of curcumin were reversed by the mitophagy inhibitor Mdivi-1.Conclusion:Curcumin effectively alleviates cell damage in BMECs under OGD/R conditions by enhancing mitophagy through the activation of the PINK1/Parkin pathway.

Objective To investigate the effects of bone marrow mesenchymal stromal cells (BMSCs) transplantation after mannitol pretreatment on behavioral performance and synaptophysin expression in the CA3 region in hippocampus of vascular dementia (VD) rats.Methods The BMSCs of rats were isolated and purified by the whole bone marrow adherence method.The rats were subjected for permanent ligation of bilateral common carotid arteries at an interval of 3 days for each carotid artery.At the same time,Sham group was set in parallel.Four weeks after modeling,the VD rats were divided randomly into five groups:(1) VD control group; (2) culture media group; (3) mannitol group; (4) BMSCs group;(5) mannitol with BMSCs group.Morris water maze performance and synaptophysin expression in the CA3 region in hippocampus were observed at 4 weeks after transplantation.Results The morris water maze performance significantly improved in mannitol with BMSCs group when compared with BMSCs group,VD control group,culture media group,mannitol group.Moreover,the escape latency of fifth day decreased significantly ((9.3 ±2.9),(14.1 ±3.5),(23.5 ±4.4),(22.8 ±4.4),(23.2 ±2.8) s,F =43.900,P =0.000)),and the platform quadrant residence time increased significantly ((40.8 ± 6.3),(34.9 ±5.8),(26.4±4.8),(27.4 ±7.0),(28.5 ±6.2) s,F=13.000,P=0.000)).The synaptophysin expressions of the hippocampal CA3 region were significantly increased in the mannitol with BMSCs group (39 624 ± 7798) when compared with BMSCs group,VD control group,culture media group,mannitol group (27060 ±4668,18 294 ±6446,19 956 ±4244,18 946 ±4953,F =39.206,P =0.000).Conclusions Intravenous BMSCs transplantation after mannitol pretreatment improves the behavioral performance of VD rats and facilitates the synaptophysin expression of hippocampal CA3 region in VD rats than BMSCs transplantation alone.Mannitol pretreatment can amplify the therapeutic effect of intravenous BMSCs transplantation in VD rats.